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The map label for this gene is ubiE [H]

Identifier: 87198619

GI number: 87198619

Start: 640532

End: 641269

Strand: Reverse

Name: ubiE [H]

Synonym: Saro_0595

Alternate gene names: 87198619

Gene position: 641269-640532 (Counterclockwise)

Preceding gene: 87198621

Following gene: 87198618

Centisome position: 18.01

GC content: 65.18

Gene sequence:

>738_bases
ATGACCAGCCCCGAAACCGCATCGTTTGGCTACGAAGACATTCCCGCAGAGGAGAAGGAAGCCCGCGTCGGCGCGATCTT
CTCCAATGTGGCGCGCAAGTACGACGTGATGAACGACGCGATGTCCGTGGGCATGCACCGCCTGTGGAAGGACAAGTTCG
TGCGCCGCGTGCGTCCCCGCGCGGGCGAGGCGATCCTCGACATGGCCGGCGGCACCGGCGACATCGCCTTCCGCATGGCG
CCTTCGGGCGCCGCGATCACCGTGTCCGACATCAACCAGGACATGCTGAATGTCGGCATGGAACGCGCCGAGAAGCGCGG
CCTGACCGGCCTCGAATGGTCACGCCAGAACGCCGAGGAACTGACCTTCCCGGACCGTGCGTTCGACGCCTACACCATCG
CTTTCGGCATCCGCAACGTGACCCACGTCGACCGCGCGCTTGCCGAGGCGTACCGCGTCCTCAAGTTCGGCGGCCGCTTC
TTCTGCCTCGAGTTCTCGACGACCGAATGGCCCGGCTTTGCCGAAGTCTACGATGCCTACTCGCACAAGCTGGTCCCCAG
GATCGGCCAGATGATCGCGGGCGATGCCGATTCCTACCGCTACCTGATCGAATCGATCCGCCGCTTTCCGCCCATGCCCG
AATTCGAGCGCATGATCCGCGCCGCGGGCTTCACCCGGACGAAGGTCGAACCCATCCTCGGCGGCCTTGTCGCGATCCAC
TCGGGGTGGAAGGCCTGA

Upstream 100 bases:

>100_bases
GGCCACGGACGGTCGTTTCTACTTCGGGAAGTTCAGGCATGACCCGCCAACCCCTAACAGCCTTTGCCGTCCGGGCAATT
CCCCACTAAAGGCACTCGAC

Downstream 100 bases:

>100_bases
TCGCTTGACCCTCCCCGCCACCCACATCCGCCGGCTGCTCGGCTGGGGCCGCACGCTCGCGCGCCATGGCGCCCTGCGCG
GGATCGAGAACGATCCGAAC

Product: demethylmenaquinone methyltransferase / 2-octaprenyl-6-methoxy-1,4-benzoquinone methylase

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MTSPETASFGYEDIPAEEKEARVGAIFSNVARKYDVMNDAMSVGMHRLWKDKFVRRVRPRAGEAILDMAGGTGDIAFRMA
PSGAAITVSDINQDMLNVGMERAEKRGLTGLEWSRQNAEELTFPDRAFDAYTIAFGIRNVTHVDRALAEAYRVLKFGGRF
FCLEFSTTEWPGFAEVYDAYSHKLVPRIGQMIAGDADSYRYLIESIRRFPPMPEFERMIRAAGFTRTKVEPILGGLVAIH
SGWKA

Sequences:

>Translated_245_residues
MTSPETASFGYEDIPAEEKEARVGAIFSNVARKYDVMNDAMSVGMHRLWKDKFVRRVRPRAGEAILDMAGGTGDIAFRMA
PSGAAITVSDINQDMLNVGMERAEKRGLTGLEWSRQNAEELTFPDRAFDAYTIAFGIRNVTHVDRALAEAYRVLKFGGRF
FCLEFSTTEWPGFAEVYDAYSHKLVPRIGQMIAGDADSYRYLIESIRRFPPMPEFERMIRAAGFTRTKVEPILGGLVAIH
SGWKA
>Mature_244_residues
TSPETASFGYEDIPAEEKEARVGAIFSNVARKYDVMNDAMSVGMHRLWKDKFVRRVRPRAGEAILDMAGGTGDIAFRMAP
SGAAITVSDINQDMLNVGMERAEKRGLTGLEWSRQNAEELTFPDRAFDAYTIAFGIRNVTHVDRALAEAYRVLKFGGRFF
CLEFSTTEWPGFAEVYDAYSHKLVPRIGQMIAGDADSYRYLIESIRRFPPMPEFERMIRAAGFTRTKVEPILGGLVAIHS
GWKA

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) [H]

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

Organism=Homo sapiens, GI116063536, Length=273, Percent_Identity=47.2527472527472, Blast_Score=253, Evalue=9e-68,
Organism=Escherichia coli, GI48994983, Length=246, Percent_Identity=45.5284552845528, Blast_Score=220, Evalue=5e-59,
Organism=Caenorhabditis elegans, GI17557039, Length=242, Percent_Identity=48.3471074380165, Blast_Score=232, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6323526, Length=256, Percent_Identity=41.796875, Blast_Score=189, Evalue=3e-49,
Organism=Drosophila melanogaster, GI24641702, Length=257, Percent_Identity=50.9727626459144, Blast_Score=266, Evalue=1e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27492; Mature: 27360

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS01183 UBIE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPETASFGYEDIPAEEKEARVGAIFSNVARKYDVMNDAMSVGMHRLWKDKFVRRVRPR
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
AGEAILDMAGGTGDIAFRMAPSGAAITVSDINQDMLNVGMERAEKRGLTGLEWSRQNAEE
CCHHHHHCCCCCCCEEEEECCCCCEEEHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCHHH
LTFPDRAFDAYTIAFGIRNVTHVDRALAEAYRVLKFGGRFFCLEFSTTEWPGFAEVYDAY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHH
SHKLVPRIGQMIAGDADSYRYLIESIRRFPPMPEFERMIRAAGFTRTKVEPILGGLVAIH
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHH
SGWKA
CCCCC
>Mature Secondary Structure 
TSPETASFGYEDIPAEEKEARVGAIFSNVARKYDVMNDAMSVGMHRLWKDKFVRRVRPR
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
AGEAILDMAGGTGDIAFRMAPSGAAITVSDINQDMLNVGMERAEKRGLTGLEWSRQNAEE
CCHHHHHCCCCCCCEEEEECCCCCEEEHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCHHH
LTFPDRAFDAYTIAFGIRNVTHVDRALAEAYRVLKFGGRFFCLEFSTTEWPGFAEVYDAY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHH
SHKLVPRIGQMIAGDADSYRYLIESIRRFPPMPEFERMIRAAGFTRTKVEPILGGLVAIH
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHH
SGWKA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA