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The map label for this gene is caiD [H]

Identifier: 87198535

GI number: 87198535

Start: 552466

End: 553236

Strand: Reverse

Name: caiD [H]

Synonym: Saro_0511

Alternate gene names: 87198535

Gene position: 553236-552466 (Counterclockwise)

Preceding gene: 87198536

Following gene: 87198534

Centisome position: 15.53

GC content: 66.15

Gene sequence:

>771_bases
ATGGCTTCGGTCGAAACGGTCGCCTCCGGGCGGGTGGGATACGTCACCCTGAACCGGCCCGAATCCCTGAACGCCATCGA
CGACGAGATGGACCGGCTGCTCGGCGATGCCTGGGCGCGGCTTGAAGCCGACAGCGACGTCTGGGTCATCGTCCTGCGCG
CAAAGGGCGAACGCGCCTTCTGCGCGGGCGGCGACATGAACGCACCGCCGACGGGCTACGAGGGGCTGTCGTTCGGAGGA
GGACTTACTGGGATCGGCGGACAACTGCGTCCGGTAAGCAAGCCGCTGATCTGCGCCGTCCACGGCCACGTTCTCGGCCT
GGGGTTCGAACTGGCGATGTGCGCGGACATCATCGTCGCGGCAAAGGGCACAAGGTTCGCGCTGCCCGAAGCGCGCGCCG
GCGTGATCGAACATTGCGGCGTCGTCCACCGCGCAATCCGGCAGCTGCCTCACCATGTTGCAATGGCAATGATCGTCGCA
GGCGAGCCACTGGACCATGAGCAGGCGTACCAGTTCGGGCTCGTCAACGAGATCGTCGAGTTCACGCAGATCGACGATGC
GGTCGAGCGTTGGACGGCGAAAGTCCTCGCCTGCTCCCCACTCGTGACACGGGCAGCCAAGGAAGCGGCCCTGACCGGAC
TGCAAGCCCCCCTGCAGGCGGCCGTTTCCCTCAGCTACCCGAGAATCGAACGCTACGCCCACAGCAGGGATCGGCGCGAA
GCCAGTGAGGCATGGGCGCAACGCCGTCAGCCGAACTGGACCGGCGAATAG

Upstream 100 bases:

>100_bases
GAACCTCGTGCTCTTTCTGGCCTCGGACGAGTCCTCCTACATCACCGGCTCAGAGCATCTGGTCGATGCCGGCATGCTGG
CCCACTGAGGGCGCCGAACG

Downstream 100 bases:

>100_bases
GCACAAACCTAACCTCCATTTTCGCAAAGTCCGCATAAAACGCCATGTGCGACGCTGAGAAACCCGGCGTAATTTCAAGC
ATTTTCGCCATATAGTTAGG

Product: enoyl-CoA hydratase/isomerase

Products: L-Carnitine.; L-carnitinyl-CoA [C]

Alternate protein names: Crotonobetainyl-CoA hydratase [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MASVETVASGRVGYVTLNRPESLNAIDDEMDRLLGDAWARLEADSDVWVIVLRAKGERAFCAGGDMNAPPTGYEGLSFGG
GLTGIGGQLRPVSKPLICAVHGHVLGLGFELAMCADIIVAAKGTRFALPEARAGVIEHCGVVHRAIRQLPHHVAMAMIVA
GEPLDHEQAYQFGLVNEIVEFTQIDDAVERWTAKVLACSPLVTRAAKEAALTGLQAPLQAAVSLSYPRIERYAHSRDRRE
ASEAWAQRRQPNWTGE

Sequences:

>Translated_256_residues
MASVETVASGRVGYVTLNRPESLNAIDDEMDRLLGDAWARLEADSDVWVIVLRAKGERAFCAGGDMNAPPTGYEGLSFGG
GLTGIGGQLRPVSKPLICAVHGHVLGLGFELAMCADIIVAAKGTRFALPEARAGVIEHCGVVHRAIRQLPHHVAMAMIVA
GEPLDHEQAYQFGLVNEIVEFTQIDDAVERWTAKVLACSPLVTRAAKEAALTGLQAPLQAAVSLSYPRIERYAHSRDRRE
ASEAWAQRRQPNWTGE
>Mature_255_residues
ASVETVASGRVGYVTLNRPESLNAIDDEMDRLLGDAWARLEADSDVWVIVLRAKGERAFCAGGDMNAPPTGYEGLSFGGG
LTGIGGQLRPVSKPLICAVHGHVLGLGFELAMCADIIVAAKGTRFALPEARAGVIEHCGVVHRAIRQLPHHVAMAMIVAG
EPLDHEQAYQFGLVNEIVEFTQIDDAVERWTAKVLACSPLVTRAAKEAALTGLQAPLQAAVSLSYPRIERYAHSRDRREA
SEAWAQRRQPNWTGE

Specific function: Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=252, Percent_Identity=30.952380952381, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI4502327, Length=246, Percent_Identity=27.2357723577236, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI31542718, Length=247, Percent_Identity=25.1012145748988, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI37594469, Length=137, Percent_Identity=28.4671532846715, Blast_Score=64, Evalue=9e-11,
Organism=Homo sapiens, GI37594471, Length=137, Percent_Identity=28.4671532846715, Blast_Score=64, Evalue=1e-10,
Organism=Escherichia coli, GI221142681, Length=262, Percent_Identity=32.4427480916031, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1787659, Length=245, Percent_Identity=31.0204081632653, Blast_Score=121, Evalue=4e-29,
Organism=Escherichia coli, GI1787660, Length=256, Percent_Identity=31.640625, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87082183, Length=258, Percent_Identity=27.5193798449612, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788597, Length=254, Percent_Identity=26.3779527559055, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1790281, Length=177, Percent_Identity=28.2485875706215, Blast_Score=64, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17554946, Length=254, Percent_Identity=31.8897637795276, Blast_Score=124, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI25145438, Length=249, Percent_Identity=30.5220883534137, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17540714, Length=228, Percent_Identity=27.1929824561404, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25144157, Length=191, Percent_Identity=29.3193717277487, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI25144160, Length=191, Percent_Identity=29.3193717277487, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17560910, Length=201, Percent_Identity=29.3532338308458, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17534483, Length=204, Percent_Identity=25.4901960784314, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17549921, Length=201, Percent_Identity=25.8706467661692, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI20129971, Length=246, Percent_Identity=30.0813008130081, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24653477, Length=246, Percent_Identity=30.0813008130081, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24653139, Length=251, Percent_Identity=29.8804780876494, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI19922422, Length=244, Percent_Identity=26.2295081967213, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21357171, Length=183, Percent_Identity=30.6010928961749, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI28571729, Length=189, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28571730, Length=189, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022852
- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.- [C]

Molecular weight: Translated: 27646; Mature: 27515

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASVETVASGRVGYVTLNRPESLNAIDDEMDRLLGDAWARLEADSDVWVIVLRAKGERAF
CCCCHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCEEE
CAGGDMNAPPTGYEGLSFGGGLTGIGGQLRPVSKPLICAVHGHVLGLGFELAMCADIIVA
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHH
AKGTRFALPEARAGVIEHCGVVHRAIRQLPHHVAMAMIVAGEPLDHEQAYQFGLVNEIVE
CCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
FTQIDDAVERWTAKVLACSPLVTRAAKEAALTGLQAPLQAAVSLSYPRIERYAHSRDRRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
ASEAWAQRRQPNWTGE
HHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ASVETVASGRVGYVTLNRPESLNAIDDEMDRLLGDAWARLEADSDVWVIVLRAKGERAF
CCCHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCEEE
CAGGDMNAPPTGYEGLSFGGGLTGIGGQLRPVSKPLICAVHGHVLGLGFELAMCADIIVA
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHH
AKGTRFALPEARAGVIEHCGVVHRAIRQLPHHVAMAMIVAGEPLDHEQAYQFGLVNEIVE
CCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
FTQIDDAVERWTAKVLACSPLVTRAAKEAALTGLQAPLQAAVSLSYPRIERYAHSRDRRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
ASEAWAQRRQPNWTGE
HHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-Carnitine; D-carnitinyl-CoA [C]

Specific reaction: D-Carnitine = L-Carnitine. D-carnitinyl-CoA = L-carnitinyl-CoA [C]

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA