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The map label for this gene is fumB [H]

Identifier: 87198214

GI number: 87198214

Start: 191828

End: 193351

Strand: Direct

Name: fumB [H]

Synonym: Saro_0188

Alternate gene names: 87198214

Gene position: 191828-193351 (Clockwise)

Preceding gene: 87198208

Following gene: 87198215

Centisome position: 5.39

GC content: 65.03

Gene sequence:

>1524_bases
ATGGCGGAAATGGTGACGATCAAGGAAGCGGACCTGATCGAAAGCGTTGCGGATGCGCTTCAATACATCAGCTATTTCCA
CCCGATGGACTACATCCGCGCGCTGGGTGAGGCCTACAAGGCCGAGCAGGGGCCAGCGGCCAAGGACGCCATCGCCCAGA
TCCTCACCAACAGCCGCATGTGCGCCGAAGGGCATCGCCCGATCTGCCAGGACACCGGCATCGTCAACGTCTTCATCGAG
TGGGGCCAGGACTGCCGCCTCGAAAGCGACCTTTCGTTGCAGGATGTGGTCGATGAGGGCGTGCGCCGTGCCTACAACAA
TCCCGACAACAAGCTGCGCGCGTCGGTTCTTGCCGATCCCGCATTCACCCGCCGCAACACCCGCGACAACACGCCCTGTG
TGCTGCACGTGGAAATGGTCAAGGGCAACAAGGTGACGGTCGATGTTGCCGCCAAGGGAGGCGGGTCGGAGAACAAGTCG
AAGTTCAAGATGATGAACCCCAGCGATTCGATCGTCGACTGGGTGATCGAGATGCTGCCCCAGATGGGCGCCGGCTGGTG
CCCGCCGGGCATGCTCGGCATTGGCATCGGCGGCACCGCGGAACACTGCGTGCTGCTCGCCAAGAAGGCGCTGATGGAGC
CGATCGATATGGCCCAGCTCAAGCAGCGCGGGCCGCAGACCGACATCGAACGCCTGCGCATCGAGATCTTCGACAAGGTC
AACGCGCTTGGCATCGGCGCGCAGGGGCTGGGCGGGCTTTCCACCATCCTCGACGTCAAGATCATGGACGCGCCGTGCCA
TGCCGCCGGCAAGCCGGTGGCGATGATCCCCAATTGCGCGGCAACCCGCCATGCGCACTTCACGCTCGACGGGTCGGGGC
CGACCTTCCTCGAGCGACCCAAGCTCGATGAATGGCCGGAAGTGCACTGGGCGCCGGACTCGGCGGCAAGGCGCGTCGAT
CTCGATACGCTGACGCAGGAAGAGGTGCAGAGCTGGAAGGCGGGCGACCGCCTGCTCCTGAACGGCAAGATGCTGACGGG
CCGCGATGCCGCGCACAAGCGGATCAAGGACATGCTCGCCAAGGGCGAGGAACTGCCCGTCGATTTCAAGGGCCGCGTGA
TCTACTACGTCGGCCCGGTCGATCCGGTGCGCGACGAAGTCGTCGGCCCGGCCGGCCCCACCACGGCCACCCGCATGGAC
AGCTTCTCCGACACCATGCTCGACCTCGGCCTGCTCGCCAGCGTCGGCAAGGCCGAGCGTGGCCCTGCCGCGACCCAGTC
CATCGCCAACCACAAGAGCGCCTATCTGATGGCGGTGGGCGGAGCGGCCTATCTTGTCGCCCGCGCGATCAAGGAGGCGA
AGGTCGTCGGCTTCGAGGACCTCGGCATGGAGGCGATCTACGAATTCACCGTCGAGGACATGCCGGTGACCGTGGCCGTC
GATTCCGGGGGCCAGAACGTTCACCAGCTCGCGCCGCTGGTGTGGAAGAAGAAGATCGCCGAGGAACACCTCCTTCCGGC
CTGA

Upstream 100 bases:

>100_bases
CTGTAGATTATCGACCTGCCGTTTCCAAGATGTTTGCTTGTCGCAAATTGCCGCGTATTGGCCTGCGCGGTTCACACGGA
CATCTGAGGGAGTCGGCAAG

Downstream 100 bases:

>100_bases
CCTTCCTTTCTTTCGCTTCGATGAACCGCATATCCCGCCCGACCACCGTTCTGGTGCGTCGGGCGGTTCGCGTTTAGGCT
GAACGCCATGACGTTGTCGC

Product: fumarase

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 507; Mature: 506

Protein sequence:

>507_residues
MAEMVTIKEADLIESVADALQYISYFHPMDYIRALGEAYKAEQGPAAKDAIAQILTNSRMCAEGHRPICQDTGIVNVFIE
WGQDCRLESDLSLQDVVDEGVRRAYNNPDNKLRASVLADPAFTRRNTRDNTPCVLHVEMVKGNKVTVDVAAKGGGSENKS
KFKMMNPSDSIVDWVIEMLPQMGAGWCPPGMLGIGIGGTAEHCVLLAKKALMEPIDMAQLKQRGPQTDIERLRIEIFDKV
NALGIGAQGLGGLSTILDVKIMDAPCHAAGKPVAMIPNCAATRHAHFTLDGSGPTFLERPKLDEWPEVHWAPDSAARRVD
LDTLTQEEVQSWKAGDRLLLNGKMLTGRDAAHKRIKDMLAKGEELPVDFKGRVIYYVGPVDPVRDEVVGPAGPTTATRMD
SFSDTMLDLGLLASVGKAERGPAATQSIANHKSAYLMAVGGAAYLVARAIKEAKVVGFEDLGMEAIYEFTVEDMPVTVAV
DSGGQNVHQLAPLVWKKKIAEEHLLPA

Sequences:

>Translated_507_residues
MAEMVTIKEADLIESVADALQYISYFHPMDYIRALGEAYKAEQGPAAKDAIAQILTNSRMCAEGHRPICQDTGIVNVFIE
WGQDCRLESDLSLQDVVDEGVRRAYNNPDNKLRASVLADPAFTRRNTRDNTPCVLHVEMVKGNKVTVDVAAKGGGSENKS
KFKMMNPSDSIVDWVIEMLPQMGAGWCPPGMLGIGIGGTAEHCVLLAKKALMEPIDMAQLKQRGPQTDIERLRIEIFDKV
NALGIGAQGLGGLSTILDVKIMDAPCHAAGKPVAMIPNCAATRHAHFTLDGSGPTFLERPKLDEWPEVHWAPDSAARRVD
LDTLTQEEVQSWKAGDRLLLNGKMLTGRDAAHKRIKDMLAKGEELPVDFKGRVIYYVGPVDPVRDEVVGPAGPTTATRMD
SFSDTMLDLGLLASVGKAERGPAATQSIANHKSAYLMAVGGAAYLVARAIKEAKVVGFEDLGMEAIYEFTVEDMPVTVAV
DSGGQNVHQLAPLVWKKKIAEEHLLPA
>Mature_506_residues
AEMVTIKEADLIESVADALQYISYFHPMDYIRALGEAYKAEQGPAAKDAIAQILTNSRMCAEGHRPICQDTGIVNVFIEW
GQDCRLESDLSLQDVVDEGVRRAYNNPDNKLRASVLADPAFTRRNTRDNTPCVLHVEMVKGNKVTVDVAAKGGGSENKSK
FKMMNPSDSIVDWVIEMLPQMGAGWCPPGMLGIGIGGTAEHCVLLAKKALMEPIDMAQLKQRGPQTDIERLRIEIFDKVN
ALGIGAQGLGGLSTILDVKIMDAPCHAAGKPVAMIPNCAATRHAHFTLDGSGPTFLERPKLDEWPEVHWAPDSAARRVDL
DTLTQEEVQSWKAGDRLLLNGKMLTGRDAAHKRIKDMLAKGEELPVDFKGRVIYYVGPVDPVRDEVVGPAGPTTATRMDS
FSDTMLDLGLLASVGKAERGPAATQSIANHKSAYLMAVGGAAYLVARAIKEAKVVGFEDLGMEAIYEFTVEDMPVTVAVD
SGGQNVHQLAPLVWKKKIAEEHLLPA

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=455, Percent_Identity=31.2087912087912, Blast_Score=188, Evalue=5e-49,
Organism=Escherichia coli, GI1787897, Length=457, Percent_Identity=31.2910284463895, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1789442, Length=288, Percent_Identity=28.125, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1789443, Length=179, Percent_Identity=29.0502793296089, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 55048; Mature: 54917

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEMVTIKEADLIESVADALQYISYFHPMDYIRALGEAYKAEQGPAAKDAIAQILTNSRM
CCCEEECCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCH
CAEGHRPICQDTGIVNVFIEWGQDCRLESDLSLQDVVDEGVRRAYNNPDNKLRASVLADP
HHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCC
AFTRRNTRDNTPCVLHVEMVKGNKVTVDVAAKGGGSENKSKFKMMNPSDSIVDWVIEMLP
HHHCCCCCCCCCEEEEEEEECCCEEEEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHH
QMGAGWCPPGMLGIGIGGTAEHCVLLAKKALMEPIDMAQLKQRGPQTDIERLRIEIFDKV
HHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHH
NALGIGAQGLGGLSTILDVKIMDAPCHAAGKPVAMIPNCAATRHAHFTLDGSGPTFLERP
HHEECCCCCCCCHHHHHEEEEECCCCCCCCCCEEECCCCCCCCCEEEEECCCCCCCCCCC
KLDEWPEVHWAPDSAARRVDLDTLTQEEVQSWKAGDRLLLNGKMLTGRDAAHKRIKDMLA
CCCCCCCCEECCCHHHCCCCHHHHHHHHHHHHCCCCEEEEECEEECCCHHHHHHHHHHHH
KGEELPVDFKGRVIYYVGPVDPVRDEVVGPAGPTTATRMDSFSDTMLDLGLLASVGKAER
CCCCCCCCCCCEEEEEECCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
GPAATQSIANHKSAYLMAVGGAAYLVARAIKEAKVVGFEDLGMEAIYEFTVEDMPVTVAV
CCHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHCCHHHHCHHHHHHHHHCCCCEEEEE
DSGGQNVHQLAPLVWKKKIAEEHLLPA
CCCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AEMVTIKEADLIESVADALQYISYFHPMDYIRALGEAYKAEQGPAAKDAIAQILTNSRM
CCEEECCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCH
CAEGHRPICQDTGIVNVFIEWGQDCRLESDLSLQDVVDEGVRRAYNNPDNKLRASVLADP
HHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCC
AFTRRNTRDNTPCVLHVEMVKGNKVTVDVAAKGGGSENKSKFKMMNPSDSIVDWVIEMLP
HHHCCCCCCCCCEEEEEEEECCCEEEEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHH
QMGAGWCPPGMLGIGIGGTAEHCVLLAKKALMEPIDMAQLKQRGPQTDIERLRIEIFDKV
HHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHH
NALGIGAQGLGGLSTILDVKIMDAPCHAAGKPVAMIPNCAATRHAHFTLDGSGPTFLERP
HHEECCCCCCCCHHHHHEEEEECCCCCCCCCCEEECCCCCCCCCEEEEECCCCCCCCCCC
KLDEWPEVHWAPDSAARRVDLDTLTQEEVQSWKAGDRLLLNGKMLTGRDAAHKRIKDMLA
CCCCCCCCEECCCHHHCCCCHHHHHHHHHHHHCCCCEEEEECEEECCCHHHHHHHHHHHH
KGEELPVDFKGRVIYYVGPVDPVRDEVVGPAGPTTATRMDSFSDTMLDLGLLASVGKAER
CCCCCCCCCCCEEEEEECCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
GPAATQSIANHKSAYLMAVGGAAYLVARAIKEAKVVGFEDLGMEAIYEFTVEDMPVTVAV
CCHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHCCHHHHCHHHHHHHHHCCCCEEEEE
DSGGQNVHQLAPLVWKKKIAEEHLLPA
CCCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]