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The map label for this gene is purD [H]

Identifier: 87198198

GI number: 87198198

Start: 172861

End: 174150

Strand: Direct

Name: purD [H]

Synonym: Saro_0172

Alternate gene names: 87198198

Gene position: 172861-174150 (Clockwise)

Preceding gene: 87198192

Following gene: 87198204

Centisome position: 4.85

GC content: 65.5

Gene sequence:

>1290_bases
ATGAATATCCTGCTGCTGGGATCGGGTGGGCGTGAACATGCCCTGGCATGGAAATTGGCCCAATCGCCCCTCACGGGCAA
GCTCTATGCCGCCCCGGGCAACCCGGGCATCGCCCAGCACGCCGATCTGGTCGCGCTTGACCTGACCGATCATGCGAGCG
TCGTCACCTTCTGCGAGACCAACCGCATCGGGCTGGTCGTCGTCGGCCCGGAAGCGCCGCTGGTCGATGGCCTGACGGAT
TCGCTGCGCGCGGCGGGTTTTTCGGTGTTCGGGCCAAGCCGTGCGGCCGCGCAGCTCGAAGGATCGAAGGGCTTTACCAA
GGACCTGTGCGCGCGGGCGGATATCCCGACGGCAGGATATGTCCGCGCGAAGTCGCTTGAAGAAGCGCGGGCCGCGCTGG
CCGAGTTCGGCGCTCCGGTCGTGATCAAGGCCGACGGGCTGGCCGCGGGCAAGGGCGTTGTCGTGGCAATGACCATGGAA
GAAGCCGAGGCAGCGGTCGACGACATGTTCGATGGCGCATTCGGTTCGGCCGGCGCGGAAGTGGTGGTCGAGGAGTTCCT
TACCGGTGAGGAGGCTAGCTTCTTCGCCCTGACCGACGGCGCCACGATCGTGCCTTTTGCTTCCGCTCAGGATCACAAGC
GCGTGGGCGATGGCGATACCGGACCCAACACCGGCGGCATGGGCGCCTACAGCCCGGCGCGCGTGCTGACCCCGGAACTC
GAGGCGCAGGCGCTTTCGCAGATCATTGCTCCGACCGTAAAGGCCATGGCGGACGAGGGCATGCCTTATTCAGGCGTGCT
CTATGCTGGCCTCATGCTCACGGAACAGGGCCCCAAGCTGATCGAATACAACGCGCGCTTCGGCGATCCCGAATGCCAGG
TGCTGATGATGCGCCTCGAGAGCGATCTGGTCGAACTGCTGCTGGCCTGCGCCGACAACAAGCTGTCGAGCATACAGCCG
CCGCGCTTCTCCAACGAAGTGGCGATGACCGTGGTCATGGCCGCGCAGGGCTATCCCGGCACGCCGAAGAAGGGCGGCCG
GATCGATCATATCCCGGCAGCAGAGGCCGGCGGCGCGAAAGTCTTCCATGCTGGCACCGTTCTTTCGGGCGATGGCGTCC
TTTCGGCCAATGGCGGGCGCGTCCTGAATGTCACGGCCAAGGGACCGACTGTAAAGTCCGCGCGCGATGCCGCCTATGCG
GCGGTCGATGCGATCAGCTTTCCGGAAGGCTTCTGCCGCCGAGACATCGGCTGGCGCGAGATCGAGCGCGAGGAAGCCGC
TAGCGTCTGA

Upstream 100 bases:

>100_bases
GATCGAGAGGGCGGGAGCGTTGTCGCCAGCCGATTCCCTCGCTAGGAGCCCGCCCGAGCCGTTGTTATCGTCGTCGTCAT
AGGGCGCGGAAGGGAAAGCC

Downstream 100 bases:

>100_bases
GGAAGGCCAGGGCCTCCGCCTCGCCGATCACTTCGGCGTACTTGGCCTGGAGATCGAACAGGTTGGCCTCGTGCGGGGCC
TGGTGGCGGTCACCGCAGGC

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MNILLLGSGGREHALAWKLAQSPLTGKLYAAPGNPGIAQHADLVALDLTDHASVVTFCETNRIGLVVVGPEAPLVDGLTD
SLRAAGFSVFGPSRAAAQLEGSKGFTKDLCARADIPTAGYVRAKSLEEARAALAEFGAPVVIKADGLAAGKGVVVAMTME
EAEAAVDDMFDGAFGSAGAEVVVEEFLTGEEASFFALTDGATIVPFASAQDHKRVGDGDTGPNTGGMGAYSPARVLTPEL
EAQALSQIIAPTVKAMADEGMPYSGVLYAGLMLTEQGPKLIEYNARFGDPECQVLMMRLESDLVELLLACADNKLSSIQP
PRFSNEVAMTVVMAAQGYPGTPKKGGRIDHIPAAEAGGAKVFHAGTVLSGDGVLSANGGRVLNVTAKGPTVKSARDAAYA
AVDAISFPEGFCRRDIGWREIEREEAASV

Sequences:

>Translated_429_residues
MNILLLGSGGREHALAWKLAQSPLTGKLYAAPGNPGIAQHADLVALDLTDHASVVTFCETNRIGLVVVGPEAPLVDGLTD
SLRAAGFSVFGPSRAAAQLEGSKGFTKDLCARADIPTAGYVRAKSLEEARAALAEFGAPVVIKADGLAAGKGVVVAMTME
EAEAAVDDMFDGAFGSAGAEVVVEEFLTGEEASFFALTDGATIVPFASAQDHKRVGDGDTGPNTGGMGAYSPARVLTPEL
EAQALSQIIAPTVKAMADEGMPYSGVLYAGLMLTEQGPKLIEYNARFGDPECQVLMMRLESDLVELLLACADNKLSSIQP
PRFSNEVAMTVVMAAQGYPGTPKKGGRIDHIPAAEAGGAKVFHAGTVLSGDGVLSANGGRVLNVTAKGPTVKSARDAAYA
AVDAISFPEGFCRRDIGWREIEREEAASV
>Mature_429_residues
MNILLLGSGGREHALAWKLAQSPLTGKLYAAPGNPGIAQHADLVALDLTDHASVVTFCETNRIGLVVVGPEAPLVDGLTD
SLRAAGFSVFGPSRAAAQLEGSKGFTKDLCARADIPTAGYVRAKSLEEARAALAEFGAPVVIKADGLAAGKGVVVAMTME
EAEAAVDDMFDGAFGSAGAEVVVEEFLTGEEASFFALTDGATIVPFASAQDHKRVGDGDTGPNTGGMGAYSPARVLTPEL
EAQALSQIIAPTVKAMADEGMPYSGVLYAGLMLTEQGPKLIEYNARFGDPECQVLMMRLESDLVELLLACADNKLSSIQP
PRFSNEVAMTVVMAAQGYPGTPKKGGRIDHIPAAEAGGAKVFHAGTVLSGDGVLSANGGRVLNVTAKGPTVKSARDAAYA
AVDAISFPEGFCRRDIGWREIEREEAASV

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=424, Percent_Identity=48.3490566037736, Blast_Score=376, Evalue=1e-104,
Organism=Homo sapiens, GI209869995, Length=424, Percent_Identity=48.3490566037736, Blast_Score=376, Evalue=1e-104,
Organism=Homo sapiens, GI209869993, Length=424, Percent_Identity=48.3490566037736, Blast_Score=376, Evalue=1e-104,
Organism=Homo sapiens, GI28416899, Length=425, Percent_Identity=48.2352941176471, Blast_Score=375, Evalue=1e-104,
Organism=Escherichia coli, GI1790438, Length=429, Percent_Identity=56.8764568764569, Blast_Score=470, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI17567511, Length=423, Percent_Identity=41.371158392435, Blast_Score=303, Evalue=8e-83,
Organism=Saccharomyces cerevisiae, GI6321203, Length=451, Percent_Identity=42.5720620842572, Blast_Score=332, Evalue=9e-92,
Organism=Drosophila melanogaster, GI62471619, Length=415, Percent_Identity=45.7831325301205, Blast_Score=310, Evalue=2e-84,
Organism=Drosophila melanogaster, GI24582400, Length=415, Percent_Identity=45.7831325301205, Blast_Score=309, Evalue=2e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 44424; Mature: 44424

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILLLGSGGREHALAWKLAQSPLTGKLYAAPGNPGIAQHADLVALDLTDHASVVTFCET
CEEEEECCCCCCCCEEEEECCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCEEEEEEEC
NRIGLVVVGPEAPLVDGLTDSLRAAGFSVFGPSRAAAQLEGSKGFTKDLCARADIPTAGY
CCEEEEEECCCCCCCCCHHHHHHHCCEEEECCCCHHHEECCCCCCHHHHHHHCCCCCCCC
VRAKSLEEARAALAEFGAPVVIKADGLAAGKGVVVAMTMEEAEAAVDDMFDGAFGSAGAE
EEHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCCHH
VVVEEFLTGEEASFFALTDGATIVPFASAQDHKRVGDGDTGPNTGGMGAYSPARVLTPEL
HHHHHHHCCCCCEEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
EAQALSQIIAPTVKAMADEGMPYSGVLYAGLMLTEQGPKLIEYNARFGDPECQVLMMRLE
HHHHHHHHHHHHHHHHHHCCCCCCCHHEEEEEEECCCCCEEEECCCCCCCHHHHHHHHHH
SDLVELLLACADNKLSSIQPPRFSNEVAMTVVMAAQGYPGTPKKGGRIDHIPAAEAGGAK
HHHHHHHHHHCCCCHHCCCCCCCCCCEEEEEEEEECCCCCCCCCCCEECCCCCCCCCCCE
VFHAGTVLSGDGVLSANGGRVLNVTAKGPTVKSARDAAYAAVDAISFPEGFCRRDIGWRE
EEEECEEEECCCEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHCCCCHHH
IEREEAASV
HHHHHHCCC
>Mature Secondary Structure
MNILLLGSGGREHALAWKLAQSPLTGKLYAAPGNPGIAQHADLVALDLTDHASVVTFCET
CEEEEECCCCCCCCEEEEECCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCEEEEEEEC
NRIGLVVVGPEAPLVDGLTDSLRAAGFSVFGPSRAAAQLEGSKGFTKDLCARADIPTAGY
CCEEEEEECCCCCCCCCHHHHHHHCCEEEECCCCHHHEECCCCCCHHHHHHHCCCCCCCC
VRAKSLEEARAALAEFGAPVVIKADGLAAGKGVVVAMTMEEAEAAVDDMFDGAFGSAGAE
EEHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCCHH
VVVEEFLTGEEASFFALTDGATIVPFASAQDHKRVGDGDTGPNTGGMGAYSPARVLTPEL
HHHHHHHCCCCCEEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
EAQALSQIIAPTVKAMADEGMPYSGVLYAGLMLTEQGPKLIEYNARFGDPECQVLMMRLE
HHHHHHHHHHHHHHHHHHCCCCCCCHHEEEEEEECCCCCEEEECCCCCCCHHHHHHHHHH
SDLVELLLACADNKLSSIQPPRFSNEVAMTVVMAAQGYPGTPKKGGRIDHIPAAEAGGAK
HHHHHHHHHHCCCCHHCCCCCCCCCCEEEEEEEEECCCCCCCCCCCEECCCCCCCCCCCE
VFHAGTVLSGDGVLSANGGRVLNVTAKGPTVKSARDAAYAAVDAISFPEGFCRRDIGWRE
EEEECEEEECCCEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHCCCCHHH
IEREEAASV
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]