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The map label for this gene is citA [H]

Identifier: 87198186

GI number: 87198186

Start: 156827

End: 158104

Strand: Direct

Name: citA [H]

Synonym: Saro_0160

Alternate gene names: 87198186

Gene position: 156827-158104 (Clockwise)

Preceding gene: 87198185

Following gene: 87198187

Centisome position: 4.4

GC content: 66.2

Gene sequence:

>1278_bases
GTGGAACCGACATCATCCGAGCCGCGCCACTTGCCCACGAAGCGGCAATTCGCGGCAGTCATTTCCGGCAATGCACTGGA
ATTCTATGATTTTCTGAGCTTCAGCTTCTTCGCGGTAAATCTGTCGCGGGTCATGTTTCCGGCAGGGGCGCCGGGCAGTG
CGCTGCTGCTGACGCTCATGACCGCGGGCGCGGGCTTCGGCGCGCGGCCCTTGGGGGCGATCCTGTTCGGAGTGCTGGGC
GACCGGATCGGACGCCGCCCGACCATGCTGGCGACTTTCGCGCTGATGGGGGTGAGCGCGCTCGGGCTCGCCCTGACGCC
GGATTACGCGGCGATCGGACCGGCAGCGCCGATCCTCGTCGTTCTGTTCCGTCTTCTGCAGGGCCTTGCCGCCGGTGGCG
ACGTGGGGCCGACGACAGCGTTCCTCGCCGAAAGCGCGCCGTCCGAAAGACGCGGGATGCTGATCGCGCTGCAGCTTGTC
GCGATGCGCATGGGAGTTCTGGCGAGCGGACTGGTGGGGCTGGTCCTGGCGAGTGTCCTGACGCCAGCGCAGCTCGACAG
TTTCGGCTGGCGCATCGCCTTTGCCATCGGTGCGGGTATCGTGCCGTTGGCTTTCATCCTGCGCCGCAGGCTCGACGAGA
CGCTTCATATGCCGGAAACCGGTCCTGACGTGGTGACCGAATTGGCCCCGCGCGCCTACGCGGCGGCGCTCTTGGGGGTA
TGCGGCTTCCTGTTGGCAGGTGCGGCGGGGGATTTCCTGTTCATCTACGCAGTGTCGTTCCTGAAGATCGCGGTGACCAA
CGGTTACATCGTGCAGATGGCGGCGGCGGGAACGCAGATCGTCGGGCTGGTGCTTGGTGGATGGCTCGGGGACCGCATCG
GGCGACGGCGGGTGAATCTCGTCACAGCAATACTGGCGGCGCTGACTTCGTTGCCGCTGTTCCGCTGGGGCATCGAGGGA
AGCGCACCGGCTCGGTTCGGCGTGGCGGCGGCCCTTCTCCTGCTCATGGCGACGGTTTCGGCTGCGGTTGCCTATGCCGC
GTTCGTCGAGACGACGCCCAAGCGGCATCGTGCGGGGCTTGTCGGCATCGGTTATGGCGTGATGGTCGCGCTGACCTTTG
GCCTGACACCAGTGGTATTGACCCGGTACATGACCGCGACCGGCGACCTCGCGGCACCCGGCTATGCCTTCGTGGTCGCC
GCGCTTTTGCTGGTGGCCTCTGCGCTGCTTCTGCCCGAGCGGAGACCCCGGCACATCGGGAAGGTTCGTTTCACCTGA

Upstream 100 bases:

>100_bases
CGGGATTTGCCGAAGTGCGCCCAGTCGTTGAAATCCGCGGCCACTGTTCCGAGTGTGCAAGCGGAGAATGAAATCGACCG
CGTAATGGGGGGGACGCAGC

Downstream 100 bases:

>100_bases
TCCCGTTCGACACCGCGCGCGCATGCGTGTAAGGGCGGTCGGATGAGCCAAGAACCGGCAACGCCGTTGCTAGACACGGT
CAAGACCCCAGACGACCTTC

Product: general substrate transporter

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter; Citrate utilization determinant; Citrate utilization protein A [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MEPTSSEPRHLPTKRQFAAVISGNALEFYDFLSFSFFAVNLSRVMFPAGAPGSALLLTLMTAGAGFGARPLGAILFGVLG
DRIGRRPTMLATFALMGVSALGLALTPDYAAIGPAAPILVVLFRLLQGLAAGGDVGPTTAFLAESAPSERRGMLIALQLV
AMRMGVLASGLVGLVLASVLTPAQLDSFGWRIAFAIGAGIVPLAFILRRRLDETLHMPETGPDVVTELAPRAYAAALLGV
CGFLLAGAAGDFLFIYAVSFLKIAVTNGYIVQMAAAGTQIVGLVLGGWLGDRIGRRRVNLVTAILAALTSLPLFRWGIEG
SAPARFGVAAALLLLMATVSAAVAYAAFVETTPKRHRAGLVGIGYGVMVALTFGLTPVVLTRYMTATGDLAAPGYAFVVA
ALLLVASALLLPERRPRHIGKVRFT

Sequences:

>Translated_425_residues
MEPTSSEPRHLPTKRQFAAVISGNALEFYDFLSFSFFAVNLSRVMFPAGAPGSALLLTLMTAGAGFGARPLGAILFGVLG
DRIGRRPTMLATFALMGVSALGLALTPDYAAIGPAAPILVVLFRLLQGLAAGGDVGPTTAFLAESAPSERRGMLIALQLV
AMRMGVLASGLVGLVLASVLTPAQLDSFGWRIAFAIGAGIVPLAFILRRRLDETLHMPETGPDVVTELAPRAYAAALLGV
CGFLLAGAAGDFLFIYAVSFLKIAVTNGYIVQMAAAGTQIVGLVLGGWLGDRIGRRRVNLVTAILAALTSLPLFRWGIEG
SAPARFGVAAALLLLMATVSAAVAYAAFVETTPKRHRAGLVGIGYGVMVALTFGLTPVVLTRYMTATGDLAAPGYAFVVA
ALLLVASALLLPERRPRHIGKVRFT
>Mature_425_residues
MEPTSSEPRHLPTKRQFAAVISGNALEFYDFLSFSFFAVNLSRVMFPAGAPGSALLLTLMTAGAGFGARPLGAILFGVLG
DRIGRRPTMLATFALMGVSALGLALTPDYAAIGPAAPILVVLFRLLQGLAAGGDVGPTTAFLAESAPSERRGMLIALQLV
AMRMGVLASGLVGLVLASVLTPAQLDSFGWRIAFAIGAGIVPLAFILRRRLDETLHMPETGPDVVTELAPRAYAAALLGV
CGFLLAGAAGDFLFIYAVSFLKIAVTNGYIVQMAAAGTQIVGLVLGGWLGDRIGRRRVNLVTAILAALTSLPLFRWGIEG
SAPARFGVAAALLLLMATVSAAVAYAAFVETTPKRHRAGLVGIGYGVMVALTFGLTPVVLTRYMTATGDLAAPGYAFVVA
ALLLVASALLLPERRPRHIGKVRFT

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=377, Percent_Identity=33.1564986737401, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1790550, Length=418, Percent_Identity=27.511961722488, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1788292, Length=418, Percent_Identity=27.2727272727273, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1789941, Length=214, Percent_Identity=34.1121495327103, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87082231, Length=215, Percent_Identity=27.906976744186, Blast_Score=62, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 44278; Mature: 44278

Theoretical pI: Translated: 10.79; Mature: 10.79

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPTSSEPRHLPTKRQFAAVISGNALEFYDFLSFSFFAVNLSRVMFPAGAPGSALLLTLM
CCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
TAGAGFGARPLGAILFGVLGDRIGRRPTMLATFALMGVSALGLALTPDYAAIGPAAPILV
HHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEECCCCHHCCCHHHHHH
VLFRLLQGLAAGGDVGPTTAFLAESAPSERRGMLIALQLVAMRMGVLASGLVGLVLASVL
HHHHHHHHHCCCCCCCCHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TPAQLDSFGWRIAFAIGAGIVPLAFILRRRLDETLHMPETGPDVVTELAPRAYAAALLGV
CHHHHHHCCCEEHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
CGFLLAGAAGDFLFIYAVSFLKIAVTNGYIVQMAAAGTQIVGLVLGGWLGDRIGRRRVNL
HHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
VTAILAALTSLPLFRWGIEGSAPARFGVAAALLLLMATVSAAVAYAAFVETTPKRHRAGL
HHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCE
VGIGYGVMVALTFGLTPVVLTRYMTATGDLAAPGYAFVVAALLLVASALLLPERRPRHIG
EEHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
KVRFT
CCCCC
>Mature Secondary Structure
MEPTSSEPRHLPTKRQFAAVISGNALEFYDFLSFSFFAVNLSRVMFPAGAPGSALLLTLM
CCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
TAGAGFGARPLGAILFGVLGDRIGRRPTMLATFALMGVSALGLALTPDYAAIGPAAPILV
HHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEECCCCHHCCCHHHHHH
VLFRLLQGLAAGGDVGPTTAFLAESAPSERRGMLIALQLVAMRMGVLASGLVGLVLASVL
HHHHHHHHHCCCCCCCCHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TPAQLDSFGWRIAFAIGAGIVPLAFILRRRLDETLHMPETGPDVVTELAPRAYAAALLGV
CHHHHHHCCCEEHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
CGFLLAGAAGDFLFIYAVSFLKIAVTNGYIVQMAAAGTQIVGLVLGGWLGDRIGRRRVNL
HHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
VTAILAALTSLPLFRWGIEGSAPARFGVAAALLLLMATVSAAVAYAAFVETTPKRHRAGL
HHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCE
VGIGYGVMVALTFGLTPVVLTRYMTATGDLAAPGYAFVVAALLLVASALLLPERRPRHIG
EEHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
KVRFT
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]