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The map label for this gene is ysgA [C]

Identifier: 87198165

GI number: 87198165

Start: 142162

End: 142860

Strand: Direct

Name: ysgA [C]

Synonym: Saro_0139

Alternate gene names: 87198165

Gene position: 142162-142860 (Clockwise)

Preceding gene: 87198157

Following gene: 87198166

Centisome position: 3.99

GC content: 61.66

Gene sequence:

>699_bases
ATGGGCGAGAAAATCACGTTCGCAGCTCTGGATGGCGAAGGCGAAATCCCGGGCTACCTCGCAACGCCGGACGGGCCGAT
CCGCGCGGCAATCATTGTCATTCCGGAGATCTTCGGCGTGAATGCCGGGATCCGGCACAAGTGCGAAGGGTGGGCGGCAC
AGGGCTACATGGCGTTGGCGCCAGACATCTTCTGGCGATTCGCGCCGGGCGTCGAGCTTGATCCCGATGTGCCGGAACAG
ATGCAGGAGGCATTCGGGTATTTCCAGCAGTACGATGCAGACAAGGGCGTGAAGGACATCGAGGCCGCAATCCGCTTCCT
GCGCGGATACGAAGGCATCGACAAGGTCGGGCTGGTCGGCTTCTGTCTGGGCGGGCGGATGGCCTACATGGCAGCCACAC
GAACCGATATCGATGCGTCGGTGGGATACTATGGCGTGATGATCGACCAGATGCTGAACGAATCGCACGCGATCGCGCGG
CCGCTGATGTTGCACATCCCTACCGCCGATCACTTCGTCGGACCGGATGCGCAGAAGGCTATTCATGATGCCCTTGATCC
GCATCCACGGGTGACGCTCCACGACTATGAGGGGCTGGACCACGGTTTCGCGGCGGAAATGGGCAATCGCCGCGACGATG
ACGGTGCCGAGCTTGCCGACCGGCGGACGCGGGATTTCTTCGCGCAGCATCTTGGATAA

Upstream 100 bases:

>100_bases
CCCGGATGAGGCGCAAGAGTGTCATGCCAGCTTGGCAAAGTCCACGACGAGGGCCAACATGACATTTGATCAACCATTAC
AATCGACAGGATGAACAGCG

Downstream 100 bases:

>100_bases
GGCCGGCGCGGTTGCGCGGGATCAAGGCCGGCTCTGCCGCCTGCCCCTATGCGTTCAGGGCTTGGTGCGGAATCGACGGA
CATTCGGGGAGAACGATGAT

Product: carboxymethylenebutenolidase

Products: NA

Alternate protein names: Dienelactone hydrolase; DLH [H]

Number of amino acids: Translated: 232; Mature: 231

Protein sequence:

>232_residues
MGEKITFAALDGEGEIPGYLATPDGPIRAAIIVIPEIFGVNAGIRHKCEGWAAQGYMALAPDIFWRFAPGVELDPDVPEQ
MQEAFGYFQQYDADKGVKDIEAAIRFLRGYEGIDKVGLVGFCLGGRMAYMAATRTDIDASVGYYGVMIDQMLNESHAIAR
PLMLHIPTADHFVGPDAQKAIHDALDPHPRVTLHDYEGLDHGFAAEMGNRRDDDGAELADRRTRDFFAQHLG

Sequences:

>Translated_232_residues
MGEKITFAALDGEGEIPGYLATPDGPIRAAIIVIPEIFGVNAGIRHKCEGWAAQGYMALAPDIFWRFAPGVELDPDVPEQ
MQEAFGYFQQYDADKGVKDIEAAIRFLRGYEGIDKVGLVGFCLGGRMAYMAATRTDIDASVGYYGVMIDQMLNESHAIAR
PLMLHIPTADHFVGPDAQKAIHDALDPHPRVTLHDYEGLDHGFAAEMGNRRDDDGAELADRRTRDFFAQHLG
>Mature_231_residues
GEKITFAALDGEGEIPGYLATPDGPIRAAIIVIPEIFGVNAGIRHKCEGWAAQGYMALAPDIFWRFAPGVELDPDVPEQM
QEAFGYFQQYDADKGVKDIEAAIRFLRGYEGIDKVGLVGFCLGGRMAYMAATRTDIDASVGYYGVMIDQMLNESHAIARP
LMLHIPTADHFVGPDAQKAIHDALDPHPRVTLHDYEGLDHGFAAEMGNRRDDDGAELADRRTRDFFAQHLG

Specific function: Unknown

COG id: COG0412

COG function: function code Q; Dienelactone hydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dienelactone hydrolase family [H]

Homologues:

Organism=Escherichia coli, GI48994982, Length=159, Percent_Identity=30.8176100628931, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002925 [H]

Pfam domain/function: PF01738 DLH [H]

EC number: =3.1.1.45 [H]

Molecular weight: Translated: 25496; Mature: 25365

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEKITFAALDGEGEIPGYLATPDGPIRAAIIVIPEIFGVNAGIRHKCEGWAAQGYMALA
CCCEEEEEEECCCCCCCCEEECCCCCCEEEEEEECHHHCCCCCCCCCCCCCCCCCEEECC
PDIFWRFAPGVELDPDVPEQMQEAFGYFQQYDADKGVKDIEAAIRFLRGYEGIDKVGLVG
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHH
FCLGGRMAYMAATRTDIDASVGYYGVMIDQMLNESHAIARPLMLHIPTADHFVGPDAQKA
HHHCCCEEEEEHHCCCCCCCCCHHHHHHHHHHCCHHHHHCCEEEEECCCHHCCCCCHHHH
IHDALDPHPRVTLHDYEGLDHGFAAEMGNRRDDDGAELADRRTRDFFAQHLG
HHHHCCCCCCEEEECCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GEKITFAALDGEGEIPGYLATPDGPIRAAIIVIPEIFGVNAGIRHKCEGWAAQGYMALA
CCEEEEEEECCCCCCCCEEECCCCCCEEEEEEECHHHCCCCCCCCCCCCCCCCCEEECC
PDIFWRFAPGVELDPDVPEQMQEAFGYFQQYDADKGVKDIEAAIRFLRGYEGIDKVGLVG
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHH
FCLGGRMAYMAATRTDIDASVGYYGVMIDQMLNESHAIARPLMLHIPTADHFVGPDAQKA
HHHCCCEEEEEHHCCCCCCCCCHHHHHHHHHHCCHHHHHCCEEEEECCCHHCCCCCHHHH
IHDALDPHPRVTLHDYEGLDHGFAAEMGNRRDDDGAELADRRTRDFFAQHLG
HHHHCCCCCCEEEECCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8138139 [H]