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The map label for this gene is purH

Identifier: 87198132

GI number: 87198132

Start: 109446

End: 111035

Strand: Direct

Name: purH

Synonym: Saro_0106

Alternate gene names: 87198132

Gene position: 109446-111035 (Clockwise)

Preceding gene: 87198131

Following gene: 87198133

Centisome position: 3.07

GC content: 65.79

Gene sequence:

>1590_bases
GTGACCGAGGTTACCATCAAGCGGGCGCTGCTTTCGGTGTCCGACAAGTCCGGCCTTGTCGATCTGGGCAAGGCGCTGGC
CGCGCGCGGCGTCGAACTGCTGTCCACCGGCGGTACTGCCAAGGCGCTGCGCGATGCCGGCCTGGCGGTGAAGGACGTGT
CCGAGCACACCGGGTTTCCCGAAATGATGGACGGCCGCGTCAAGACGCTGCACCCGACGATCCACGGAGGCCTGCTGGCC
GTACGAGACAATCCCGAACACGCCGCTGCGATGGCCGAGCACGCGATCGGTGCGATCGATCTCGTCGTGGTCAACCTCTA
CCCGTTCGAAGCGACCGTGGCCAAGGGTGCGGAGCGTGAGGAGGTGATCGAGAACATCGACATCGGCGGACCTTCGATGG
TCCGTTCGGCGGCGAAGAACCACGAATACGTCACCATCCTGACGGATGCGGCCGACTATGCCGCGTTCCTTGGCGAACTC
GACCAGACCGGCGGCGCGACGACACTCGCCTTCCGCCGCAAGATGGCCGCCAAGGCCTATGCCGCAACCGCCGCCTATGA
TGCGGCAATCTCGCAGTGGTTCGCGACCGTGGACCAGCAGGAACACTTCCCACCCGTCCTCGCCGCCGCGCACACCCGCG
TGACCACGCTGCGCTATGGCGAGAACCCTCACCAGGATGCCGCACTCTACGTTCCGCGCAATCCCGCCGTCACCGGCCTG
CCGCAGGCGACCCAGGTGCAGGGCAAGGAGCTGTCCTACAACAACTACAACGATGCCAACGCAGCGCTTGAACTGGTCGC
CGAGTTCGCGGGCGCGAAGCCGACGGTGGTGATCGTGAAGCACGCCAACCCCTGCGGCGTGGCAACGGCGGAAACGCTGC
TTGAAGCGTGGAAGGACGCGCTCGAGTGCGACTCGGTCTCGGCATTCGGCGGGATCGTGGCGACGAACGTTCCGCTCGAC
GGGCCAACCGCAAACGCAATCTGCGAGATATTCACCGAAGTGGTTGTGGCGCCGGGCGCAGATGACGCGGCGAAGGAAGC
CTTCGCACGCAAGAAGAACCTGCGCCTGCTGCTGATCGATTCGCTTCCCGATGCGGGCCGCAAGGGCCTCGTCACCGTGC
CGATCGCGGGCGGTCTACTGGTGCAGGACCGCGATGCGGGCAAGATCGACAAGTCCGACCTCAAGGTGGTGACCAAGCGC
GCGCCGACCGAACGGGAGCTGGAGGACGCGCTCTTCGCCTGGACCGTGGCAAAGCACGTCAAGTCGAACGCCATCGTCTA
TGCCAAGGACGGCGTGACGGCCGGCATTGGCGCAGGCCAGATGAACCGCCGCGACAGCTCGCGCATCGCCGCCGCGAAGG
CCAGGGAAGCGGCCGAAACCCATCACTTCAGCGAAGTGCGCACGGTAGGCTCGGCAGTAGCCTCCGACGCGTTCTTCCCC
TTTGCCGATGGCCTGATGGCGGCCGTGGAAGCGGGAGCCACCTGCGTGATCCAGCCCGGTGGCTCGATCCGCGACGAGGA
TGTGATCAAGGCTGCTGATGAAGCCGGCCTTGCCATGGTCTTCACCGGAATGCGCCACTTCCGCCACTGA

Upstream 100 bases:

>100_bases
CGCCCGCTGGCAACGCGCCAGCTTGTCGTCACAGCCAAGGTTCCACGCAGCGGAGAGAGCTTCTCCTGGCTGCTCAAGAA
GATGAGATAGGAATTTCCAC

Downstream 100 bases:

>100_bases
CCTGAATCCCGCCCGGGCGCGCATCAGCGAGGCCCGGGCGGAAGCGGCCCAATATCGTCCTTCGTGCAAATGCCTGATTG
CGCTAGGCAACAGGCGTGCT

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MTEVTIKRALLSVSDKSGLVDLGKALAARGVELLSTGGTAKALRDAGLAVKDVSEHTGFPEMMDGRVKTLHPTIHGGLLA
VRDNPEHAAAMAEHAIGAIDLVVVNLYPFEATVAKGAEREEVIENIDIGGPSMVRSAAKNHEYVTILTDAADYAAFLGEL
DQTGGATTLAFRRKMAAKAYAATAAYDAAISQWFATVDQQEHFPPVLAAAHTRVTTLRYGENPHQDAALYVPRNPAVTGL
PQATQVQGKELSYNNYNDANAALELVAEFAGAKPTVVIVKHANPCGVATAETLLEAWKDALECDSVSAFGGIVATNVPLD
GPTANAICEIFTEVVVAPGADDAAKEAFARKKNLRLLLIDSLPDAGRKGLVTVPIAGGLLVQDRDAGKIDKSDLKVVTKR
APTERELEDALFAWTVAKHVKSNAIVYAKDGVTAGIGAGQMNRRDSSRIAAAKAREAAETHHFSEVRTVGSAVASDAFFP
FADGLMAAVEAGATCVIQPGGSIRDEDVIKAADEAGLAMVFTGMRHFRH

Sequences:

>Translated_529_residues
MTEVTIKRALLSVSDKSGLVDLGKALAARGVELLSTGGTAKALRDAGLAVKDVSEHTGFPEMMDGRVKTLHPTIHGGLLA
VRDNPEHAAAMAEHAIGAIDLVVVNLYPFEATVAKGAEREEVIENIDIGGPSMVRSAAKNHEYVTILTDAADYAAFLGEL
DQTGGATTLAFRRKMAAKAYAATAAYDAAISQWFATVDQQEHFPPVLAAAHTRVTTLRYGENPHQDAALYVPRNPAVTGL
PQATQVQGKELSYNNYNDANAALELVAEFAGAKPTVVIVKHANPCGVATAETLLEAWKDALECDSVSAFGGIVATNVPLD
GPTANAICEIFTEVVVAPGADDAAKEAFARKKNLRLLLIDSLPDAGRKGLVTVPIAGGLLVQDRDAGKIDKSDLKVVTKR
APTERELEDALFAWTVAKHVKSNAIVYAKDGVTAGIGAGQMNRRDSSRIAAAKAREAAETHHFSEVRTVGSAVASDAFFP
FADGLMAAVEAGATCVIQPGGSIRDEDVIKAADEAGLAMVFTGMRHFRH
>Mature_528_residues
TEVTIKRALLSVSDKSGLVDLGKALAARGVELLSTGGTAKALRDAGLAVKDVSEHTGFPEMMDGRVKTLHPTIHGGLLAV
RDNPEHAAAMAEHAIGAIDLVVVNLYPFEATVAKGAEREEVIENIDIGGPSMVRSAAKNHEYVTILTDAADYAAFLGELD
QTGGATTLAFRRKMAAKAYAATAAYDAAISQWFATVDQQEHFPPVLAAAHTRVTTLRYGENPHQDAALYVPRNPAVTGLP
QATQVQGKELSYNNYNDANAALELVAEFAGAKPTVVIVKHANPCGVATAETLLEAWKDALECDSVSAFGGIVATNVPLDG
PTANAICEIFTEVVVAPGADDAAKEAFARKKNLRLLLIDSLPDAGRKGLVTVPIAGGLLVQDRDAGKIDKSDLKVVTKRA
PTERELEDALFAWTVAKHVKSNAIVYAKDGVTAGIGAGQMNRRDSSRIAAAKAREAAETHHFSEVRTVGSAVASDAFFPF
ADGLMAAVEAGATCVIQPGGSIRDEDVIKAADEAGLAMVFTGMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=609, Percent_Identity=35.7963875205254, Blast_Score=290, Evalue=3e-78,
Organism=Escherichia coli, GI1790439, Length=539, Percent_Identity=53.9888682745826, Blast_Score=536, Evalue=1e-153,
Organism=Caenorhabditis elegans, GI71985564, Length=607, Percent_Identity=32.7841845140033, Blast_Score=267, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI71985574, Length=315, Percent_Identity=27.6190476190476, Blast_Score=77, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323768, Length=607, Percent_Identity=33.1136738056013, Blast_Score=278, Evalue=1e-75,
Organism=Saccharomyces cerevisiae, GI6323056, Length=608, Percent_Identity=33.0592105263158, Blast_Score=273, Evalue=6e-74,
Organism=Drosophila melanogaster, GI24649832, Length=474, Percent_Identity=38.6075949367089, Blast_Score=257, Evalue=2e-68,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 55750; Mature: 55618

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEVTIKRALLSVSDKSGLVDLGKALAARGVELLSTGGTAKALRDAGLAVKDVSEHTGFP
CCCHHHHHHHHHCCCCCCHHHHHHHHHHCCHHEEECCCHHHHHHHCCCEEEECHHHCCCH
EMMDGRVKTLHPTIHGGLLAVRDNPEHAAAMAEHAIGAIDLVVVNLYPFEATVAKGAERE
HHHCCCEEEECCCCCCCEEEEECCCHHHHHHHHHHCCEEEEEEEEEECCHHHHHCCCHHH
EVIENIDIGGPSMVRSAAKNHEYVTILTDAADYAAFLGELDQTGGATTLAFRRKMAAKAY
HHHHHCCCCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
AATAAYDAAISQWFATVDQQEHFPPVLAAAHTRVTTLRYGENPHQDAALYVPRNPAVTGL
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHEEEEEECCCCCCCCCEEEECCCCCCCCC
PQATQVQGKELSYNNYNDANAALELVAEFAGAKPTVVIVKHANPCGVATAETLLEAWKDA
CCHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
LECDSVSAFGGIVATNVPLDGPTANAICEIFTEVVVAPGADDAAKEAFARKKNLRLLLID
HCCCCCHHHCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEEE
SLPDAGRKGLVTVPIAGGLLVQDRDAGKIDKSDLKVVTKRAPTERELEDALFAWTVAKHV
CCCCCCCCCEEEEEECCCEEEECCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
KSNAIVYAKDGVTAGIGAGQMNRRDSSRIAAAKAREAAETHHFSEVRTVGSAVASDAFFP
CCCEEEEECCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
FADGLMAAVEAGATCVIQPGGSIRDEDVIKAADEAGLAMVFTGMRHFRH
HHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TEVTIKRALLSVSDKSGLVDLGKALAARGVELLSTGGTAKALRDAGLAVKDVSEHTGFP
CCHHHHHHHHHCCCCCCHHHHHHHHHHCCHHEEECCCHHHHHHHCCCEEEECHHHCCCH
EMMDGRVKTLHPTIHGGLLAVRDNPEHAAAMAEHAIGAIDLVVVNLYPFEATVAKGAERE
HHHCCCEEEECCCCCCCEEEEECCCHHHHHHHHHHCCEEEEEEEEEECCHHHHHCCCHHH
EVIENIDIGGPSMVRSAAKNHEYVTILTDAADYAAFLGELDQTGGATTLAFRRKMAAKAY
HHHHHCCCCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
AATAAYDAAISQWFATVDQQEHFPPVLAAAHTRVTTLRYGENPHQDAALYVPRNPAVTGL
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHEEEEEECCCCCCCCCEEEECCCCCCCCC
PQATQVQGKELSYNNYNDANAALELVAEFAGAKPTVVIVKHANPCGVATAETLLEAWKDA
CCHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
LECDSVSAFGGIVATNVPLDGPTANAICEIFTEVVVAPGADDAAKEAFARKKNLRLLLID
HCCCCCHHHCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEEE
SLPDAGRKGLVTVPIAGGLLVQDRDAGKIDKSDLKVVTKRAPTERELEDALFAWTVAKHV
CCCCCCCCCEEEEEECCCEEEECCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
KSNAIVYAKDGVTAGIGAGQMNRRDSSRIAAAKAREAAETHHFSEVRTVGSAVASDAFFP
CCCEEEEECCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
FADGLMAAVEAGATCVIQPGGSIRDEDVIKAADEAGLAMVFTGMRHFRH
HHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA