BacMap

Definition	Novosphingobium aromaticivorans DSM 12444 chromosome, complete genome.
Accession	NC_007794
Length	3,561,584

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The map label for this gene is dnaJ [H]

Identifier: 87198075

GI number: 87198075

Start: 51839

End: 52816

Strand: Direct

Name: dnaJ [H]

Synonym: Saro_0049

Alternate gene names: 87198075

Gene position: 51839-52816 (Clockwise)

Preceding gene: 87198071

Following gene: 87198076

Centisome position: 1.46

GC content: 67.48

Gene sequence:

>978_bases
ATGGCCGATCCGTATTCGATCCTGGGGGTTCCGCGCAGCGCAAGCGAAAAGGACATCAAGTCCGCCTATCGCAAGCTTGC
CAAGGAACTGCACCCGGACACCAACAAGGACAATCCCAAGGCTGCGGAGCGCTTCTCGGAAGTGACCCGCGCCTATGACC
TCCTGTCCGACAAGGACAAGCGCGCCCGCTTCGACCGGGGCGAGATCGATGCCGAAGGCAACCCGACCGGGCCGTTCGGC
GGCGGCTTCGGCGGCGGTTTCGGCGGAGGCCAACGAGGCACGCAAGGCGGCTTCCGCCATGACGGAGGCTTCGAGGGCGG
CTTTGGTGGCGGGCCTGAAGGCATCGACATCGGCGACATATTCGAGGGCCTGTTCGGCGGGCGCGGCGGCATGGGTGCGG
GCGGAGGCGGCGCCGGAATGGGCGGCGGCTTCGGCGGCGCGAGGCGTGGGCCGCCTCCACGGGGCGCGAACGTCAACTAT
CGCCTGCAGGTCTCGTTCGTCGACGCGGCGACGCGCGCCAACCAGCGTATTACCCTGTCGGATGGCAAGACCATCGATCT
CAAGCTGCCCGCGGGCCTCGAAAGCGGCCAGCAGATGCGCCTTGCCGGAAAGGGAGAGCCCGGTCCGGGCGGCAACGGCG
ATGCCATCGTGACGATCGAGATCGGCAACCACCCGTTCTACGAACGCGATGGCGACAATATCCGCCTCGACCTGCCGATC
AGCCTTTCCGAGGCGGTTCACGGCGCGAAGGTCAAGGTGCCGACCGTCGACGGCGCGGTCATGCTGACCGTAGCGCCAGG
CAGTTCGAGCGGCCGCACCCTGCGCCTCAAGGGCAAGGGCTTCACCCGCAAGGACGGCGCCCGCGGCGACCAGCTCGTGA
CCCTGCAGATCGACATTCCCGCCGACGACGCGGATCTGAAAGCGCGTCTCGAGGGTTGGCAGGACATGCGGAACCTGCGC
GACAAACTCGGAGCCTGA

Upstream 100 bases:

>100_bases
CAATGACCGGGATCAATTGCATGGACACCGGACTACTCCTGCCGGGCGGAGTAGGAAAGGCAACCTTGCGGGCAAGCCCC
GCAAATCCTAGCTATGGCGC

Downstream 100 bases:

>100_bases
CGAATGGAGCCCGGCCACCTCCCGCCGGGCGCGCACCCGCTCGTCGAGGTTCCCGACCTTTCGCCCGAAGCGCGACGGCG
CGAAGCGCTGCGCTGGCGCG

Product: heat shock protein DnaJ-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MADPYSILGVPRSASEKDIKSAYRKLAKELHPDTNKDNPKAAERFSEVTRAYDLLSDKDKRARFDRGEIDAEGNPTGPFG
GGFGGGFGGGQRGTQGGFRHDGGFEGGFGGGPEGIDIGDIFEGLFGGRGGMGAGGGGAGMGGGFGGARRGPPPRGANVNY
RLQVSFVDAATRANQRITLSDGKTIDLKLPAGLESGQQMRLAGKGEPGPGGNGDAIVTIEIGNHPFYERDGDNIRLDLPI
SLSEAVHGAKVKVPTVDGAVMLTVAPGSSSGRTLRLKGKGFTRKDGARGDQLVTLQIDIPADDADLKARLEGWQDMRNLR
DKLGA

Sequences:

>Translated_325_residues
MADPYSILGVPRSASEKDIKSAYRKLAKELHPDTNKDNPKAAERFSEVTRAYDLLSDKDKRARFDRGEIDAEGNPTGPFG
GGFGGGFGGGQRGTQGGFRHDGGFEGGFGGGPEGIDIGDIFEGLFGGRGGMGAGGGGAGMGGGFGGARRGPPPRGANVNY
RLQVSFVDAATRANQRITLSDGKTIDLKLPAGLESGQQMRLAGKGEPGPGGNGDAIVTIEIGNHPFYERDGDNIRLDLPI
SLSEAVHGAKVKVPTVDGAVMLTVAPGSSSGRTLRLKGKGFTRKDGARGDQLVTLQIDIPADDADLKARLEGWQDMRNLR
DKLGA
>Mature_324_residues
ADPYSILGVPRSASEKDIKSAYRKLAKELHPDTNKDNPKAAERFSEVTRAYDLLSDKDKRARFDRGEIDAEGNPTGPFGG
GFGGGFGGGQRGTQGGFRHDGGFEGGFGGGPEGIDIGDIFEGLFGGRGGMGAGGGGAGMGGGFGGARRGPPPRGANVNYR
LQVSFVDAATRANQRITLSDGKTIDLKLPAGLESGQQMRLAGKGEPGPGGNGDAIVTIEIGNHPFYERDGDNIRLDLPIS
LSEAVHGAKVKVPTVDGAVMLTVAPGSSSGRTLRLKGKGFTRKDGARGDQLVTLQIDIPADDADLKARLEGWQDMRNLRD
KLGA

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, dnaK-independent fashion. Unfolded proteins bind initially to dnaJ; upon

COG id: COG2214

COG function: function code O; DnaJ-class molecular chaperone

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 J domain [H]

Homologues:

Organism=Homo sapiens, GI205360840, Length=353, Percent_Identity=27.7620396600567, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI7706495, Length=326, Percent_Identity=29.4478527607362, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI6631085, Length=334, Percent_Identity=30.8383233532934, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI205360838, Length=355, Percent_Identity=28.4507042253521, Blast_Score=114, Evalue=8e-26,
Organism=Homo sapiens, GI201862587, Length=346, Percent_Identity=28.6127167630058, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI201862518, Length=346, Percent_Identity=28.6127167630058, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI56549115, Length=346, Percent_Identity=28.6127167630058, Blast_Score=111, Evalue=9e-25,
Organism=Homo sapiens, GI4504511, Length=327, Percent_Identity=25.3822629969419, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI5453690, Length=340, Percent_Identity=28.2352941176471, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI39204547, Length=277, Percent_Identity=29.2418772563177, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI56687498, Length=64, Percent_Identity=53.125, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI47777312, Length=132, Percent_Identity=43.9393939393939, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI88501736, Length=105, Percent_Identity=48.5714285714286, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI27151736, Length=105, Percent_Identity=48.5714285714286, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI23503241, Length=91, Percent_Identity=46.1538461538462, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI24308127, Length=116, Percent_Identity=37.0689655172414, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI4885495, Length=67, Percent_Identity=53.7313432835821, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI5031741, Length=62, Percent_Identity=54.8387096774194, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI17388799, Length=67, Percent_Identity=53.7313432835821, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI9558755, Length=61, Percent_Identity=52.4590163934426, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI21553335, Length=67, Percent_Identity=49.2537313432836, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI45504382, Length=62, Percent_Identity=46.7741935483871, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI68077168, Length=88, Percent_Identity=44.3181818181818, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI68077166, Length=88, Percent_Identity=44.3181818181818, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI8922563, Length=64, Percent_Identity=43.75, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI29126218, Length=62, Percent_Identity=46.7741935483871, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI1787235, Length=331, Percent_Identity=31.4199395770393, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1786197, Length=335, Percent_Identity=30.7462686567164, Blast_Score=149, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI212645553, Length=325, Percent_Identity=29.8461538461538, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17534355, Length=337, Percent_Identity=28.1899109792285, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17553096, Length=363, Percent_Identity=25.6198347107438, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17553098, Length=363, Percent_Identity=25.6198347107438, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17507263, Length=340, Percent_Identity=26.4705882352941, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17554900, Length=86, Percent_Identity=45.3488372093023, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17554644, Length=94, Percent_Identity=43.6170212765958, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI115532476, Length=64, Percent_Identity=53.125, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17538236, Length=127, Percent_Identity=40.1574803149606, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI115532378, Length=101, Percent_Identity=34.6534653465347, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324265, Length=346, Percent_Identity=25.1445086705202, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323813, Length=129, Percent_Identity=42.6356589147287, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323870, Length=72, Percent_Identity=47.2222222222222, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6322194, Length=65, Percent_Identity=44.6153846153846, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323119, Length=142, Percent_Identity=33.0985915492958, Blast_Score=63, Evalue=5e-11,
Organism=Drosophila melanogaster, GI85726398, Length=349, Percent_Identity=28.6532951289398, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24658562, Length=336, Percent_Identity=30.0595238095238, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24658555, Length=336, Percent_Identity=30.0595238095238, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI19920464, Length=323, Percent_Identity=27.5541795665635, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI18858081, Length=323, Percent_Identity=29.1021671826625, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI45549272, Length=63, Percent_Identity=53.968253968254, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45552813, Length=63, Percent_Identity=53.968253968254, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45552811, Length=63, Percent_Identity=53.968253968254, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI17737735, Length=62, Percent_Identity=53.2258064516129, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28574723, Length=62, Percent_Identity=53.2258064516129, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24668523, Length=61, Percent_Identity=54.0983606557377, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21355911, Length=101, Percent_Identity=42.5742574257426, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24654066, Length=66, Percent_Identity=51.5151515151515, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24654070, Length=66, Percent_Identity=51.5151515151515, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24654068, Length=66, Percent_Identity=51.5151515151515, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24654072, Length=66, Percent_Identity=51.5151515151515, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI161077138, Length=66, Percent_Identity=51.5151515151515, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24668492, Length=81, Percent_Identity=43.2098765432099, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24645889, Length=62, Percent_Identity=46.7741935483871, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012724
- InterPro:   IPR002939
- InterPro:   IPR001623
- InterPro:   IPR018253
- InterPro:   IPR008971
- InterPro:   IPR015609
- InterPro:   IPR003095
- InterPro:   IPR001305 [H]

Pfam domain/function: PF00226 DnaJ; PF01556 DnaJ_C; PF00684 DnaJ_CXXCXGXG [H]

EC number: NA

Molecular weight: Translated: 33863; Mature: 33732

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: PS00636 DNAJ_1 ; PS50076 DNAJ_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADPYSILGVPRSASEKDIKSAYRKLAKELHPDTNKDNPKAAERFSEVTRAYDLLSDKDK
CCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCHH
RARFDRGEIDAEGNPTGPFGGGFGGGFGGGQRGTQGGFRHDGGFEGGFGGGPEGIDIGDI
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
FEGLFGGRGGMGAGGGGAGMGGGFGGARRGPPPRGANVNYRLQVSFVDAATRANQRITLS
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEHHHCCCCEEEEC
DGKTIDLKLPAGLESGQQMRLAGKGEPGPGGNGDAIVTIEIGNHPFYERDGDNIRLDLPI
CCCEEEEECCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEECC
SLSEAVHGAKVKVPTVDGAVMLTVAPGSSSGRTLRLKGKGFTRKDGARGDQLVTLQIDIP
CHHHHCCCCEEEEEECCCEEEEEECCCCCCCCEEEEECCCCCCCCCCCCCEEEEEEEECC
ADDADLKARLEGWQDMRNLRDKLGA
CCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ADPYSILGVPRSASEKDIKSAYRKLAKELHPDTNKDNPKAAERFSEVTRAYDLLSDKDK
CCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCHH
RARFDRGEIDAEGNPTGPFGGGFGGGFGGGQRGTQGGFRHDGGFEGGFGGGPEGIDIGDI
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
FEGLFGGRGGMGAGGGGAGMGGGFGGARRGPPPRGANVNYRLQVSFVDAATRANQRITLS
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEHHHCCCCEEEEC
DGKTIDLKLPAGLESGQQMRLAGKGEPGPGGNGDAIVTIEIGNHPFYERDGDNIRLDLPI
CCCEEEEECCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEECC
SLSEAVHGAKVKVPTVDGAVMLTVAPGSSSGRTLRLKGKGFTRKDGARGDQLVTLQIDIP
CHHHHCCCCEEEEEECCCEEEEEECCCCCCCCEEEEECCCCCCCCCCCCCEEEEEEEECC
ADDADLKARLEGWQDMRNLRDKLGA
CCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA