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The map label for this gene is norM [H]

Identifier: 87198059

GI number: 87198059

Start: 33831

End: 35222

Strand: Direct

Name: norM [H]

Synonym: Saro_0033

Alternate gene names: 87198059

Gene position: 33831-35222 (Clockwise)

Preceding gene: 87198056

Following gene: 87198060

Centisome position: 0.95

GC content: 69.32

Gene sequence:

>1392_bases
ATGGCCCCGCTCCCCATGTTTGAAAAGCCGCCCGTACACTGGACCGAAGAACTGCGCGCGATGCTCCGTCTCGCCGCGCC
GATGGTCGGGGCGAACCTGCTGCAGATGGCGGTCTTTGCGGTGGACGTGGTGTTCGTGGCGCGGCTCGGGCCGGTGGCGC
TTGCCGCCTCCTCGCTGTCGGTGTCGCTCTTCGGCCTTCTCGTCTGGAGCCTCTCGGGCCTTGTCGGCGCGGCATCGCCA
CTGATCGCGGCCGAACTCGGTCGGCGCCGGCACGCCGTTCGCGAAGTGCGCAGAACCGTGCGCATGGCGGCCTGGGCGGG
AACCCTGGCTGGCCTGTTCGCCATGGGCGTCTGCCTGCTCGGCGGTCCGCTGATGCGCGTCACCGGCCAGCAGCCCGAAG
TCATCGCGCTTGCCGTCCCGTTTCTCAACGTCCTGATGTGGGCGGCGATTCCTTCCACGATCTCGGCGCTGCTGCGCACC
TTCGTGGCGACGCTCGGCCGTCCGACGATCGGCACGGTCATCACCGGCATGGCAGTGGCCATCAACGCCTTCGGCAACTG
GGTCTTCGTCTTCGGCAACCTCGGCGCGCCGGAAATGGGGCTCACCGGCTCTGCCTTGTCGAGCATAGTCACGACCTGCG
CGATGGTCCTGGCCTATATCGTGGTGATCCGCTCCGACCGCCGCCTGCGTCGCTACAGGCTGGCCGGCCGGTGGTGGAAG
CCGGAGTGGAAGCGTTTCGCCGACGTGCTGCGCGTGGGCCTGCCGATCACCGGCACGATCCTTGCCGAGGCGGGGATGTT
CAACGGCGCCGCCTTCCTGATGGGACGCATCGGCGAGGTCGAGCTTGCCGCCCATACGATCGCCCTCCAGTTCGCCGCAA
TCGCCTTCCAGGTGCCGTTCGGTGTCGCCCAGGCTGCGACCATCCGCGTGGGCCTGGCCTTCGGTGCTGGCGAGCGGGCC
GCCATCGCCCGGGCAGGCCGGGTCGCCGTCGTGCTCGGCATGGGCTTCATGGTGGTGACCGCCAGCATCATGCTCTTCGC
CCCGCGCGCGATCCTGCATCTCTACGTCGATCCCGACGCGCCCGAAAACCGCGCAATGGCCGTCCTCGCGGTGCAGTACA
TGGCGGTTGCCGCAGCGTTCCAGTTGTTCGACGGCGCGCAGGCCGTGGGCGCTGCCCTGCTGCGGGGCCTGCAGGACACG
CGTATTCCCATGGCCTTCGCCCTGTTCGGCTACTGGCTGCCCGGGCTGGGGACGGCCGTCGGCCTCGGGCTCTTCTCTCC
GCTCGGCGGGCTGGGCGTATGGATCGGCCTCATGGTCGGCCTCGTCGTCGTGGCCAGCCTCATGCTATGGCGTTGGCGGA
ACCGTGCGCGACTGGGCCTCCTCCCGGCCTGA

Upstream 100 bases:

>100_bases
TCCGCCAGCAGCGCAGCGGCCCCTGGATCGATCAACGCCGGACGGAACCAAAATTTCCGTGCCCGGAGGACTCTTCTTTC
CTTGGGGTCGCACTCGCCCT

Downstream 100 bases:

>100_bases
ACGCCAGTCGCGAAATTCGTTCATCCCGGCGAAAAGAATCTTCACGGTGCCCATTGACGGCCCATGACGAGGCACCCATA
TCCGCGCCGCTGGCACTCTC

Product: MATE efflux family protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MAPLPMFEKPPVHWTEELRAMLRLAAPMVGANLLQMAVFAVDVVFVARLGPVALAASSLSVSLFGLLVWSLSGLVGAASP
LIAAELGRRRHAVREVRRTVRMAAWAGTLAGLFAMGVCLLGGPLMRVTGQQPEVIALAVPFLNVLMWAAIPSTISALLRT
FVATLGRPTIGTVITGMAVAINAFGNWVFVFGNLGAPEMGLTGSALSSIVTTCAMVLAYIVVIRSDRRLRRYRLAGRWWK
PEWKRFADVLRVGLPITGTILAEAGMFNGAAFLMGRIGEVELAAHTIALQFAAIAFQVPFGVAQAATIRVGLAFGAGERA
AIARAGRVAVVLGMGFMVVTASIMLFAPRAILHLYVDPDAPENRAMAVLAVQYMAVAAAFQLFDGAQAVGAALLRGLQDT
RIPMAFALFGYWLPGLGTAVGLGLFSPLGGLGVWIGLMVGLVVVASLMLWRWRNRARLGLLPA

Sequences:

>Translated_463_residues
MAPLPMFEKPPVHWTEELRAMLRLAAPMVGANLLQMAVFAVDVVFVARLGPVALAASSLSVSLFGLLVWSLSGLVGAASP
LIAAELGRRRHAVREVRRTVRMAAWAGTLAGLFAMGVCLLGGPLMRVTGQQPEVIALAVPFLNVLMWAAIPSTISALLRT
FVATLGRPTIGTVITGMAVAINAFGNWVFVFGNLGAPEMGLTGSALSSIVTTCAMVLAYIVVIRSDRRLRRYRLAGRWWK
PEWKRFADVLRVGLPITGTILAEAGMFNGAAFLMGRIGEVELAAHTIALQFAAIAFQVPFGVAQAATIRVGLAFGAGERA
AIARAGRVAVVLGMGFMVVTASIMLFAPRAILHLYVDPDAPENRAMAVLAVQYMAVAAAFQLFDGAQAVGAALLRGLQDT
RIPMAFALFGYWLPGLGTAVGLGLFSPLGGLGVWIGLMVGLVVVASLMLWRWRNRARLGLLPA
>Mature_462_residues
APLPMFEKPPVHWTEELRAMLRLAAPMVGANLLQMAVFAVDVVFVARLGPVALAASSLSVSLFGLLVWSLSGLVGAASPL
IAAELGRRRHAVREVRRTVRMAAWAGTLAGLFAMGVCLLGGPLMRVTGQQPEVIALAVPFLNVLMWAAIPSTISALLRTF
VATLGRPTIGTVITGMAVAINAFGNWVFVFGNLGAPEMGLTGSALSSIVTTCAMVLAYIVVIRSDRRLRRYRLAGRWWKP
EWKRFADVLRVGLPITGTILAEAGMFNGAAFLMGRIGEVELAAHTIALQFAAIAFQVPFGVAQAATIRVGLAFGAGERAA
IARAGRVAVVLGMGFMVVTASIMLFAPRAILHLYVDPDAPENRAMAVLAVQYMAVAAAFQLFDGAQAVGAALLRGLQDTR
IPMAFALFGYWLPGLGTAVGLGLFSPLGGLGVWIGLMVGLVVVASLMLWRWRNRARLGLLPA

Specific function: Multidrug efflux pump [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family [H]

Homologues:

Organism=Homo sapiens, GI22907060, Length=439, Percent_Identity=25.5125284738041, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI153792564, Length=430, Percent_Identity=21.8604651162791, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI38261960, Length=466, Percent_Identity=21.244635193133, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI48994890, Length=451, Percent_Identity=29.0465631929047, Blast_Score=157, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 49095; Mature: 48963

Theoretical pI: Translated: 11.92; Mature: 11.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPLPMFEKPPVHWTEELRAMLRLAAPMVGANLLQMAVFAVDVVFVARLGPVALAASSLS
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
VSLFGLLVWSLSGLVGAASPLIAAELGRRRHAVREVRRTVRMAAWAGTLAGLFAMGVCLL
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGPLMRVTGQQPEVIALAVPFLNVLMWAAIPSTISALLRTFVATLGRPTIGTVITGMAVA
CCCCEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
INAFGNWVFVFGNLGAPEMGLTGSALSSIVTTCAMVLAYIVVIRSDRRLRRYRLAGRWWK
HHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCC
PEWKRFADVLRVGLPITGTILAEAGMFNGAAFLMGRIGEVELAAHTIALQFAAIAFQVPF
HHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
GVAQAATIRVGLAFGAGERAAIARAGRVAVVLGMGFMVVTASIMLFAPRAILHLYVDPDA
CHHHHHHEEEEEEECCCCHHHHHHCCCEEEHHHHHHHHHHHHHHHHCCHHHEEEEECCCC
PENRAMAVLAVQYMAVAAAFQLFDGAQAVGAALLRGLQDTRIPMAFALFGYWLPGLGTAV
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHH
GLGLFSPLGGLGVWIGLMVGLVVVASLMLWRWRNRARLGLLPA
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
APLPMFEKPPVHWTEELRAMLRLAAPMVGANLLQMAVFAVDVVFVARLGPVALAASSLS
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
VSLFGLLVWSLSGLVGAASPLIAAELGRRRHAVREVRRTVRMAAWAGTLAGLFAMGVCLL
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGPLMRVTGQQPEVIALAVPFLNVLMWAAIPSTISALLRTFVATLGRPTIGTVITGMAVA
CCCCEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
INAFGNWVFVFGNLGAPEMGLTGSALSSIVTTCAMVLAYIVVIRSDRRLRRYRLAGRWWK
HHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCC
PEWKRFADVLRVGLPITGTILAEAGMFNGAAFLMGRIGEVELAAHTIALQFAAIAFQVPF
HHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
GVAQAATIRVGLAFGAGERAAIARAGRVAVVLGMGFMVVTASIMLFAPRAILHLYVDPDA
CHHHHHHEEEEEEECCCCHHHHHHCCCEEEHHHHHHHHHHHHHHHHCCHHHEEEEECCCC
PENRAMAVLAVQYMAVAAAFQLFDGAQAVGAALLRGLQDTRIPMAFALFGYWLPGLGTAV
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHH
GLGLFSPLGGLGVWIGLMVGLVVVASLMLWRWRNRARLGLLPA
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12910271 [H]