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The map label for this gene is acyI [H]

Identifier: 86751748

GI number: 86751748

Start: 5258896

End: 5260587

Strand: Direct

Name: acyI [H]

Synonym: RPB_4650

Alternate gene names: 86751748

Gene position: 5258896-5260587 (Clockwise)

Preceding gene: 86751747

Following gene: 86751750

Centisome position: 98.64

GC content: 69.15

Gene sequence:

>1692_bases
GTGAGGATTGCCGGCGCGCTGGTTGCTGTGTTTGATGCCGGACCGCCGCGCGCGCGGCCTCGCCATCCTCGAAGACATCA
ACGAAAGCAACAGCCCGCCATGGCCGACCAGTTCAGCAACCTGCAGCAATTCCGCAAGCCCGCCGTCGTGTCCAAGGGCG
GCATCGTCGCCGCCCAGTCGCGCAAGGCGGCGGAGGTCGGCGCCGAGGTGCTGGCGGCGGGCGGCGACTGCGTCGATGCG
GTGATCGCGACCACGTTTGCGCTGGGTGTGCTGGAGCCGTGGATGAGCGGGATCGGCGGCGGCGGCGCCATGGTGCTGTA
TCGCGCCGCCGAGGACACATACGAAGTGATCGACTACGGCATGTGCGCGCCTCTCAGCCTGCGCACCGAGGACTATCCGC
TGACCGGCGCGGGCGCCGCGTCCGATCTGTTTCCGTGGCCGCGGGTGAAGGATGATCGCAACCTGCACGGACCGGGCTCG
GTCGCGGTGCCCGGCGTCGTCGCCGGCATGGAGGAAGCGCATCGCCGCCACGCGAAATTGCCGTGGAAGGAACTGATTGC
GCCGAGCATCGCGCTCGCCGGCGAAGGCCTCGCGATCGACTGGTGGACGACGCTGATGATCGGCAGCGTCGCCGCGGATC
TGCGCCGTTACGAGTCGAGCGCGGCCTCCTATCTGATCGACGGCCTGCCGCCGCATGCGCCATGGGGCATCAAGGCGAAG
GTCCAGATGCCGCAGCAGCGGCTGCAGGCGGCGATGGCACAACTCGCGGCACAAGGTCCGCGCGATTTCTACGAGGGCGA
GCTGGCGCGCAGCATCGCCGCCGACATCAAAGCCAATGGCGGCTCGTTGACCGTCGACGACCTCGCCGCGTTCCGTGCGC
ATCTGCGCGAGCCGCTGCAGATCCCGTATCGCGGCGGCACGGTGTATGCCACGCCCGAACTCACCGCCGGTCCGACGCTG
GCGCATACGCTGCGCCTGCTGCAGCAGAATCTGCACGCCAACGGCGGCGCCCCGGATGCGGAGGCCTATGCCACCTACGC
GCTGGCGCTGCAATCGGCCTATGCCGAGCGGCTGCGCGACATGGGCGACGCCGACGGCCGCCGCGCGCTCGGCGCCGAAC
ATCTGGCGCCGGCCTGCACCACGCATTTCTCGGTGGTCGATCGCCACGGCAACATGGCGGCGGTGACGCAGACGCTGCTG
TCGACCTTCGGCTCGAAATTCGAACTGCCGAGCAGCGGCATCATGATGAACAACGGCATCATGTGGTTCGATCCCGAGCC
GGGCCGCCCGAATTCGCTGGCGCCCGGCAAGCGCTGCCTCACCAACTACACGCCGGTGCTGGCGCAGGCCGCCGACGGCC
GCAGGCTCGCGCTCGGCGCGTCGGGCGGCCGGCGCATTCTGCCGGCGGTGACGCAGATGCTATCGTTCGTGATCGACTAC
GGCATGGATCTCGATGCGGCGATCCATCAGCCGCGGATCGACGCCAGCGAAGGCGCGGTCGTGATCGGCGACGTGAGGCT
GCCGCAGGCGACACGCGACGCGCTGGCGCAGCGCTTCGACTACGAGGAAGCGCGGGGGCAGACCTTCCCGATGAAATTCG
CCTGCCCGAGTGTCGTCCTGCGCGAAGGTGCCGAGAACAGTGGCGCGGTCGAAGTCGCGCAACCCTGGGGTGACGCGGTC
GCCGAGGCATAG

Upstream 100 bases:

>100_bases
AGCGCATGCCGACGAGCCGCGGATCATCCTGACCGAGCCCGGCATCGGCTATCGGATCGGCGACGCAGGCTGAGTCTCCG
CGCAAGGCAGGGTTCCGCGC

Downstream 100 bases:

>100_bases
GCGCGGCTGCATAAGGCTCGCTCATTCACAACGCGGGCGGCGGCCTGTTGAGACTACCAGGGCGGCCGCACCGACATGAA
ATGGACGGATTCGATATGGC

Product: gamma-glutamyltransferase

Products: NA

Alternate protein names: Cephalosporin acylase; GL-7ACA acylase; Gamma-glutamyltranspeptidase; GGT [H]

Number of amino acids: Translated: 563; Mature: 563

Protein sequence:

>563_residues
MRIAGALVAVFDAGPPRARPRHPRRHQRKQQPAMADQFSNLQQFRKPAVVSKGGIVAAQSRKAAEVGAEVLAAGGDCVDA
VIATTFALGVLEPWMSGIGGGGAMVLYRAAEDTYEVIDYGMCAPLSLRTEDYPLTGAGAASDLFPWPRVKDDRNLHGPGS
VAVPGVVAGMEEAHRRHAKLPWKELIAPSIALAGEGLAIDWWTTLMIGSVAADLRRYESSAASYLIDGLPPHAPWGIKAK
VQMPQQRLQAAMAQLAAQGPRDFYEGELARSIAADIKANGGSLTVDDLAAFRAHLREPLQIPYRGGTVYATPELTAGPTL
AHTLRLLQQNLHANGGAPDAEAYATYALALQSAYAERLRDMGDADGRRALGAEHLAPACTTHFSVVDRHGNMAAVTQTLL
STFGSKFELPSSGIMMNNGIMWFDPEPGRPNSLAPGKRCLTNYTPVLAQAADGRRLALGASGGRRILPAVTQMLSFVIDY
GMDLDAAIHQPRIDASEGAVVIGDVRLPQATRDALAQRFDYEEARGQTFPMKFACPSVVLREGAENSGAVEVAQPWGDAV
AEA

Sequences:

>Translated_563_residues
MRIAGALVAVFDAGPPRARPRHPRRHQRKQQPAMADQFSNLQQFRKPAVVSKGGIVAAQSRKAAEVGAEVLAAGGDCVDA
VIATTFALGVLEPWMSGIGGGGAMVLYRAAEDTYEVIDYGMCAPLSLRTEDYPLTGAGAASDLFPWPRVKDDRNLHGPGS
VAVPGVVAGMEEAHRRHAKLPWKELIAPSIALAGEGLAIDWWTTLMIGSVAADLRRYESSAASYLIDGLPPHAPWGIKAK
VQMPQQRLQAAMAQLAAQGPRDFYEGELARSIAADIKANGGSLTVDDLAAFRAHLREPLQIPYRGGTVYATPELTAGPTL
AHTLRLLQQNLHANGGAPDAEAYATYALALQSAYAERLRDMGDADGRRALGAEHLAPACTTHFSVVDRHGNMAAVTQTLL
STFGSKFELPSSGIMMNNGIMWFDPEPGRPNSLAPGKRCLTNYTPVLAQAADGRRLALGASGGRRILPAVTQMLSFVIDY
GMDLDAAIHQPRIDASEGAVVIGDVRLPQATRDALAQRFDYEEARGQTFPMKFACPSVVLREGAENSGAVEVAQPWGDAV
AEA
>Mature_563_residues
MRIAGALVAVFDAGPPRARPRHPRRHQRKQQPAMADQFSNLQQFRKPAVVSKGGIVAAQSRKAAEVGAEVLAAGGDCVDA
VIATTFALGVLEPWMSGIGGGGAMVLYRAAEDTYEVIDYGMCAPLSLRTEDYPLTGAGAASDLFPWPRVKDDRNLHGPGS
VAVPGVVAGMEEAHRRHAKLPWKELIAPSIALAGEGLAIDWWTTLMIGSVAADLRRYESSAASYLIDGLPPHAPWGIKAK
VQMPQQRLQAAMAQLAAQGPRDFYEGELARSIAADIKANGGSLTVDDLAAFRAHLREPLQIPYRGGTVYATPELTAGPTL
AHTLRLLQQNLHANGGAPDAEAYATYALALQSAYAERLRDMGDADGRRALGAEHLAPACTTHFSVVDRHGNMAAVTQTLL
STFGSKFELPSSGIMMNNGIMWFDPEPGRPNSLAPGKRCLTNYTPVLAQAADGRRLALGASGGRRILPAVTQMLSFVIDY
GMDLDAAIHQPRIDASEGAVVIGDVRLPQATRDALAQRFDYEEARGQTFPMKFACPSVVLREGAENSGAVEVAQPWGDAV
AEA

Specific function: Besides the cephalosporin acylase I activity which converts GL-7ACA into 7-ACA; this enzyme displays some gamma glutamyltranspeptidase activity [H]

COG id: COG0405

COG function: function code E; Gamma-glutamyltransferase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI73915092, Length=491, Percent_Identity=28.9205702647658, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI73915090, Length=491, Percent_Identity=28.9205702647658, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI73915096, Length=491, Percent_Identity=28.9205702647658, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI73915094, Length=491, Percent_Identity=28.9205702647658, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI113429326, Length=491, Percent_Identity=28.3095723014257, Blast_Score=144, Evalue=3e-34,
Organism=Homo sapiens, GI113429324, Length=493, Percent_Identity=27.9918864097363, Blast_Score=142, Evalue=7e-34,
Organism=Homo sapiens, GI153266885, Length=549, Percent_Identity=25.5009107468124, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI153266901, Length=550, Percent_Identity=25.4545454545455, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI153266907, Length=542, Percent_Identity=24.5387453874539, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI109148539, Length=529, Percent_Identity=26.8431001890359, Blast_Score=114, Evalue=3e-25,
Organism=Escherichia coli, GI1789856, Length=498, Percent_Identity=30.7228915662651, Blast_Score=198, Evalue=7e-52,
Organism=Caenorhabditis elegans, GI17542894, Length=495, Percent_Identity=24.8484848484848, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17506189, Length=492, Percent_Identity=25.2032520325203, Blast_Score=122, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI193208245, Length=537, Percent_Identity=23.8361266294227, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI193208247, Length=537, Percent_Identity=23.8361266294227, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI212646519, Length=514, Percent_Identity=24.9027237354086, Blast_Score=118, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI72000294, Length=509, Percent_Identity=24.5579567779961, Blast_Score=118, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6323330, Length=487, Percent_Identity=24.2299794661191, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI161077878, Length=507, Percent_Identity=27.0216962524655, Blast_Score=173, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24642670, Length=507, Percent_Identity=27.0216962524655, Blast_Score=173, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24642666, Length=507, Percent_Identity=27.0216962524655, Blast_Score=173, Evalue=3e-43,
Organism=Drosophila melanogaster, GI45551474, Length=507, Percent_Identity=27.0216962524655, Blast_Score=173, Evalue=3e-43,
Organism=Drosophila melanogaster, GI161077880, Length=507, Percent_Identity=27.0216962524655, Blast_Score=173, Evalue=3e-43,
Organism=Drosophila melanogaster, GI18859893, Length=529, Percent_Identity=27.2211720226843, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI161077496, Length=469, Percent_Identity=27.0788912579957, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI18859741, Length=566, Percent_Identity=25.4416961130742, Blast_Score=125, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000101 [H]

Pfam domain/function: PF01019 G_glu_transpept [H]

EC number: =2.3.2.2 [H]

Molecular weight: Translated: 60008; Mature: 60008

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS01109 RIBOSOMAL_L10

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAGALVAVFDAGPPRARPRHPRRHQRKQQPAMADQFSNLQQFRKPAVVSKGGIVAAQS
CCCCCEEEEEECCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCEECCCCEEEECC
RKAAEVGAEVLAAGGDCVDAVIATTFALGVLEPWMSGIGGGGAMVLYRAAEDTYEVIDYG
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHCC
MCAPLSLRTEDYPLTGAGAASDLFPWPRVKDDRNLHGPGSVAVPGVVAGMEEAHRRHAKL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
PWKELIAPSIALAGEGLAIDWWTTLMIGSVAADLRRYESSAASYLIDGLPPHAPWGIKAK
CHHHHHCCHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
VQMPQQRLQAAMAQLAAQGPRDFYEGELARSIAADIKANGGSLTVDDLAAFRAHLREPLQ
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCCC
IPYRGGTVYATPELTAGPTLAHTLRLLQQNLHANGGAPDAEAYATYALALQSAYAERLRD
CCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
MGDADGRRALGAEHLAPACTTHFSVVDRHGNMAAVTQTLLSTFGSKFELPSSGIMMNNGI
CCCCCCCHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCEEEECCE
MWFDPEPGRPNSLAPGKRCLTNYTPVLAQAADGRRLALGASGGRRILPAVTQMLSFVIDY
EEECCCCCCCCCCCCHHHHHHCCCHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHC
GMDLDAAIHQPRIDASEGAVVIGDVRLPQATRDALAQRFDYEEARGQTFPMKFACPSVVL
CCCCHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCHHHHCCCCCCCHHCCHHHHH
REGAENSGAVEVAQPWGDAVAEA
HCCCCCCCCEEEECCCCHHHCCC
>Mature Secondary Structure
MRIAGALVAVFDAGPPRARPRHPRRHQRKQQPAMADQFSNLQQFRKPAVVSKGGIVAAQS
CCCCCEEEEEECCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCEECCCCEEEECC
RKAAEVGAEVLAAGGDCVDAVIATTFALGVLEPWMSGIGGGGAMVLYRAAEDTYEVIDYG
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHCC
MCAPLSLRTEDYPLTGAGAASDLFPWPRVKDDRNLHGPGSVAVPGVVAGMEEAHRRHAKL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
PWKELIAPSIALAGEGLAIDWWTTLMIGSVAADLRRYESSAASYLIDGLPPHAPWGIKAK
CHHHHHCCHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
VQMPQQRLQAAMAQLAAQGPRDFYEGELARSIAADIKANGGSLTVDDLAAFRAHLREPLQ
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCCC
IPYRGGTVYATPELTAGPTLAHTLRLLQQNLHANGGAPDAEAYATYALALQSAYAERLRD
CCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
MGDADGRRALGAEHLAPACTTHFSVVDRHGNMAAVTQTLLSTFGSKFELPSSGIMMNNGI
CCCCCCCHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCEEEECCE
MWFDPEPGRPNSLAPGKRCLTNYTPVLAQAADGRRLALGASGGRRILPAVTQMLSFVIDY
EEECCCCCCCCCCCCHHHHHHCCCHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHC
GMDLDAAIHQPRIDASEGAVVIGDVRLPQATRDALAQRFDYEEARGQTFPMKFACPSVVL
CCCCHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCHHHHCCCCCCCHHCCHHHHH
REGAENSGAVEVAQPWGDAVAEA
HCCCCCCCCEEEECCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3680178 [H]