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The map label for this gene is dinF [H]

Identifier: 86751717

GI number: 86751717

Start: 5220130

End: 5221488

Strand: Direct

Name: dinF [H]

Synonym: RPB_4619

Alternate gene names: 86751717

Gene position: 5220130-5221488 (Clockwise)

Preceding gene: 86751716

Following gene: 86751718

Centisome position: 97.91

GC content: 68.73

Gene sequence:

>1359_bases
TTGCCTCCCGCCTCGTTCGTCACCTCCCGGCAGGTGTTCGCCATCGCCGGCCCGGCGATGGTCGCCAACCTGACGACCCC
GCTGCTCGGCGTGGTCGCCACCACGGCGATCGGACGACTCGGCGACGCCACCATGCTCGGCGGCGTGGCGATGACGTCGG
TGATCTTCGACTGCCTGTTCTGGCTGTTCGGCTTCCTGCGGATGAGCACGCTGGCGTTCACCGCGCAGGCACTCGGCGCC
GGCGACACCCGCGAAGTGCCCGCTCATCTGGTCCGCGGCCTGATCGTCGCCGCGCTGATCGGGCTGGCGCTGATCGCGTT
GCAAGCGCCGATGGCAGCCGCGCTGATCGGCGCGATGGGCGGCAGCGACGGCGTCAGCGCGGCGGCGAAGGCCTACTTCT
CCATCCGGATCTGGTCCGCGCCGCTGGTGCTGGCGAATTTCGCCGTGCTCGGCTGGCTGGTCGGGCAGGCGCGCGCCAAG
CTCGCGCTCGGCGTGCAGGTCGCGATCAACCTGATCAATATCGCGACGACGATCGCGCTGGTGCTGTGGTTCGAGTTCGG
CATCGCCGGCGCGGCGATCGCGGCGGTGATCGCCGAGGGCTCGGGGCTGGTCGTCGGGCTTGCGGTCGCGATCTGGCTGT
TCCGCGGCGTGCCGCGGCCGTCGCGCGCCGCCTTGTTCGACTCCGACAAGTTGAAGCGGATGTTCGCGGTCAACCGCGAC
ATCATGATTCGTACTGCCGCGATGATCGCGGTGTTCCTGTTCTTCACCGCGCAGGGCGCCCGCGACGGCGACGTCGTGCT
CGCCACCAACTCGGTGCTCAACAATTTCCTGATGGTCAGCGCGTTCTTTCTCGACGGCCTCGCCAACGCCGCCGAGCAGC
TCTGCGGCCGAACGCTCGGCGCACGCGACCGCGCCGGCTTCGTCGCGGCGACGCGGCTGGTGATCCTGTGGGGCTTCGGC
TTCGCGCTGGTGGTGAGCGCGACCTACGCGCTGGCCGGCCCGATGCTGATCGACGTGATGACGTCGAACGAAGAACTGCG
CCGCGCCGCGCGCGACTATCTCTGGCTGGTGACGCTGGCGCCGGTGCTCGGCGTGTTCGCCTTCGCGTTCGACGGCATCT
TCATCGGCGCCACCTGGGCGCGCGAGATGCGCAATCTGATGTTGCTGTCGCTGGTGCTGTTCTTCGCGAGCTGGCTGGCA
CTGCGCCCGCTCGGCAACACCGGGCTGTGGATCGCGCTGCTGCTGCACTACGTCTGGCGCGGCGGGCTGCAGGCGCTGCG
CTATCCGGCGATGCTACGCACCTCGTTTCCGGCTGCGACGGCCGGTTCAACTTTCCTAAAATTGACAGCACCGGATTAG

Upstream 100 bases:

>100_bases
GCGCTACTTCCTGCTCGGCGCGGGGATCGTGATCCCGATCTGGCTGCTGACTCGCCTGTTCGGCACGCCGCGCGGGAGAT
AATCCGCGCGGATCGGAATT

Downstream 100 bases:

>100_bases
CTCGGATTTCACGACAGAATGAGTTGCGGTCAAAACAGCTCTTCGCGAAATCCATGTCACCGAAATACCATCTCTCCGAG
GATCGCAAAAAGGAGAGCTG

Product: MATE efflux family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MPPASFVTSRQVFAIAGPAMVANLTTPLLGVVATTAIGRLGDATMLGGVAMTSVIFDCLFWLFGFLRMSTLAFTAQALGA
GDTREVPAHLVRGLIVAALIGLALIALQAPMAAALIGAMGGSDGVSAAAKAYFSIRIWSAPLVLANFAVLGWLVGQARAK
LALGVQVAINLINIATTIALVLWFEFGIAGAAIAAVIAEGSGLVVGLAVAIWLFRGVPRPSRAALFDSDKLKRMFAVNRD
IMIRTAAMIAVFLFFTAQGARDGDVVLATNSVLNNFLMVSAFFLDGLANAAEQLCGRTLGARDRAGFVAATRLVILWGFG
FALVVSATYALAGPMLIDVMTSNEELRRAARDYLWLVTLAPVLGVFAFAFDGIFIGATWAREMRNLMLLSLVLFFASWLA
LRPLGNTGLWIALLLHYVWRGGLQALRYPAMLRTSFPAATAGSTFLKLTAPD

Sequences:

>Translated_452_residues
MPPASFVTSRQVFAIAGPAMVANLTTPLLGVVATTAIGRLGDATMLGGVAMTSVIFDCLFWLFGFLRMSTLAFTAQALGA
GDTREVPAHLVRGLIVAALIGLALIALQAPMAAALIGAMGGSDGVSAAAKAYFSIRIWSAPLVLANFAVLGWLVGQARAK
LALGVQVAINLINIATTIALVLWFEFGIAGAAIAAVIAEGSGLVVGLAVAIWLFRGVPRPSRAALFDSDKLKRMFAVNRD
IMIRTAAMIAVFLFFTAQGARDGDVVLATNSVLNNFLMVSAFFLDGLANAAEQLCGRTLGARDRAGFVAATRLVILWGFG
FALVVSATYALAGPMLIDVMTSNEELRRAARDYLWLVTLAPVLGVFAFAFDGIFIGATWAREMRNLMLLSLVLFFASWLA
LRPLGNTGLWIALLLHYVWRGGLQALRYPAMLRTSFPAATAGSTFLKLTAPD
>Mature_451_residues
PPASFVTSRQVFAIAGPAMVANLTTPLLGVVATTAIGRLGDATMLGGVAMTSVIFDCLFWLFGFLRMSTLAFTAQALGAG
DTREVPAHLVRGLIVAALIGLALIALQAPMAAALIGAMGGSDGVSAAAKAYFSIRIWSAPLVLANFAVLGWLVGQARAKL
ALGVQVAINLINIATTIALVLWFEFGIAGAAIAAVIAEGSGLVVGLAVAIWLFRGVPRPSRAALFDSDKLKRMFAVNRDI
MIRTAAMIAVFLFFTAQGARDGDVVLATNSVLNNFLMVSAFFLDGLANAAEQLCGRTLGARDRAGFVAATRLVILWGFGF
ALVVSATYALAGPMLIDVMTSNEELRRAARDYLWLVTLAPVLGVFAFAFDGIFIGATWAREMRNLMLLSLVLFFASWLAL
RPLGNTGLWIALLLHYVWRGGLQALRYPAMLRTSFPAATAGSTFLKLTAPD

Specific function: Unknown

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790477, Length=421, Percent_Identity=37.0546318289786, Blast_Score=187, Evalue=1e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 47808; Mature: 47677

Theoretical pI: Translated: 10.77; Mature: 10.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPASFVTSRQVFAIAGPAMVANLTTPLLGVVATTAIGRLGDATMLGGVAMTSVIFDCLF
CCCCCHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
WLFGFLRMSTLAFTAQALGAGDTREVPAHLVRGLIVAALIGLALIALQAPMAAALIGAMG
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GSDGVSAAAKAYFSIRIWSAPLVLANFAVLGWLVGQARAKLALGVQVAINLINIATTIAL
CCCCHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLWFEFGIAGAAIAAVIAEGSGLVVGLAVAIWLFRGVPRPSRAALFDSDKLKRMFAVNRD
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHCCHHHHHHHHHCCCH
IMIRTAAMIAVFLFFTAQGARDGDVVLATNSVLNNFLMVSAFFLDGLANAAEQLCGRTLG
HHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ARDRAGFVAATRLVILWGFGFALVVSATYALAGPMLIDVMTSNEELRRAARDYLWLVTLA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHH
PVLGVFAFAFDGIFIGATWAREMRNLMLLSLVLFFASWLALRPLGNTGLWIALLLHYVWR
HHHHHHHHHHCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
GGLQALRYPAMLRTSFPAATAGSTFLKLTAPD
HHHHHHHHHHHHHCCCCCCCCCCCEEEEECCC
>Mature Secondary Structure 
PPASFVTSRQVFAIAGPAMVANLTTPLLGVVATTAIGRLGDATMLGGVAMTSVIFDCLF
CCCCHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
WLFGFLRMSTLAFTAQALGAGDTREVPAHLVRGLIVAALIGLALIALQAPMAAALIGAMG
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GSDGVSAAAKAYFSIRIWSAPLVLANFAVLGWLVGQARAKLALGVQVAINLINIATTIAL
CCCCHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLWFEFGIAGAAIAAVIAEGSGLVVGLAVAIWLFRGVPRPSRAALFDSDKLKRMFAVNRD
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHCCHHHHHHHHHCCCH
IMIRTAAMIAVFLFFTAQGARDGDVVLATNSVLNNFLMVSAFFLDGLANAAEQLCGRTLG
HHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ARDRAGFVAATRLVILWGFGFALVVSATYALAGPMLIDVMTSNEELRRAARDYLWLVTLA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHH
PVLGVFAFAFDGIFIGATWAREMRNLMLLSLVLFFASWLALRPLGNTGLWIALLLHYVWR
HHHHHHHHHHCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
GGLQALRYPAMLRTSFPAATAGSTFLKLTAPD
HHHHHHHHHHHHHCCCCCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503 [H]