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The map label for this gene is hyuB [H]

Identifier: 86751530

GI number: 86751530

Start: 5018602

End: 5020347

Strand: Direct

Name: hyuB [H]

Synonym: RPB_4429

Alternate gene names: 86751530

Gene position: 5018602-5020347 (Clockwise)

Preceding gene: 86751529

Following gene: 86751531

Centisome position: 94.13

GC content: 67.53

Gene sequence:

>1746_bases
ATGGACGTCCACGCCCCTCACGCCGATCCGATGAACGTGATCCATCAGCAGATCATGTGGGATCGCCTGATCTCCGTGGT
CGAAGAACAGGCCCAGACGCTGATCCGGATCGGCTTCAGCACCTCGACCCGCGAAGCCGGCGACGTCTCGGCCGGCGTGT
TCAACACCGCCGGTCACATGCTCGCGCAGGCCGTCACCGGCACGCCCGGCCACGTCAATTCGATGGCGCGCGCGGTGGTG
CATTTCCTCGACAAGTTTCCCGCGACGACGATGCAGCCCGGCGACATCTTCATCACCAACGATCCGTGGAAGGGAACCGG
CCATCTCCACGACTTCACGGTGGTGACGCCCGTCTTCCGCGCCGACCGGCTGGTCGCGCTGTTCGCCAGCACCTGCCACG
TCATCGACATCGGCGGACGCGGCATGGGCCCGGACGCCCGCCAAGTGTTCGAGGAAGGCGTCTTCATCCCGATCATGCGC
TTCGCCAGCGCCGCCGGCACCAACGAGACGCTGCTCGAGATCATCAAGGGCAATGTCCGGGAGCCCGTGCAGGTCGTCGG
CGACCTCTATTCGCTGGCCGGCTGCAACGATGTCGGCGGCCGGCAGCTCCTGAAGATGATGGACGAATTCGGTATCGAGA
CTCTCGACCGTCTCGGCGATCACATTCTGGAACGGTCACGCGTCGCCACGCTGGAAGCGATCCGCGCGCTGCCGAAAGGT
ACGTTCCGCAACAGCATGCGCGTCGACGGCTACGACAGGCCGCTCGATCTGGTCGCGACGATGACGATCTCCGACGACGG
CATCGATGTCGATTTCGCCGGCACCTCGCCGCCCTCGTCCTTCGGCATCAACGTGCCGTTCTGCTACACCGAGGCCTATG
CGAGCTTCGGCGTGAAATGCATCATCGCACCGAAGATCCCGAACAACGAGGGCTCGCTCGCCTTGCTGCGCATGCGCGCA
CCGGCGGATTGCATCCTCAACGCGCAGCCGCCGCTGCCCGTCGCCACGCGCCATATCGTCGGACAGATGTTGCCCGATCT
GGTGATCGGCTGCCTCGGCCAGGCGCTTCACGGCAACGTCCCGGCGGAAGGCACCTCCTGTCTTTGGAACCTGTTCGCCT
TCGGCGGCTCCAGCCAGATCGACGCCGACTCCACCGAGATGATGCGGGCCCGCGTGTTCAACGTGATGTCGTTCCATTCC
GGCGGCACCGGCGCGCGGCCGGGCAAGGACGGCCTGTCGGCCACCGCCTTCCCGAGCGGCGTGCGCAACGTGCCGGTGGA
GGTCACCGAGGCGATGTCGCCGCTGCTGATCAAGCGCAAGGAATATCGCACCGACTCCGGCGGCCCCGGCCAATTCCGCG
GCGGCCTCGGCCAGGTGATGGAAGTCGTCAGCCTCGACGACACCGCCTTCGCGATCTCCGCCAACTACGACCGCGTCGAT
TTCCCCGCCCGCGGCCGCGACGGCGGCGCCGACGGCAAGGCCGGCAAGATCTCGCTCGGCTCCGGCAGGCTGCTGAAGAG
CAAGGGCCAGCAGACCATTCCGCGCGGCGAAGCCGTGCTGATCGAAATGCCCGGCGGCGGCGGCCTCGGCGATCCCTTCA
GCCGCGATGCCGCGGCGGTCGCCGCGGACGTGCATCTCGGCATGGTGTCGCGCGAGGCCGCCGAGACGGCCTACGGCGTC
GTGCTGCGCGGCGACCACTCCGTCGACGAAACCGCGACCGCCGCACGCCGCGGCCATCGCGCCTGA

Upstream 100 bases:

>100_bases
CCGCGCTGATCGTCGAGGACGAAACCACGACGATGGTGACCCACCGCTACACCGCCCGCATCGACGCGCTCGGCAGCATC
GTCATGACGAGGACCTGATC

Downstream 100 bases:

>100_bases
TCCGATCCACACCGATTCCACATTCGCCGAGGAAACCGAAGATGAGCCTGATCCTGAGCGCCGGCCTGCCCACTGGAATG
GAAGGCCTCACCTATCCGAT

Product: 5-oxoprolinase (ATP-hydrolyzing)

Products: NA

Alternate protein names: ORF3 [H]

Number of amino acids: Translated: 581; Mature: 581

Protein sequence:

>581_residues
MDVHAPHADPMNVIHQQIMWDRLISVVEEQAQTLIRIGFSTSTREAGDVSAGVFNTAGHMLAQAVTGTPGHVNSMARAVV
HFLDKFPATTMQPGDIFITNDPWKGTGHLHDFTVVTPVFRADRLVALFASTCHVIDIGGRGMGPDARQVFEEGVFIPIMR
FASAAGTNETLLEIIKGNVREPVQVVGDLYSLAGCNDVGGRQLLKMMDEFGIETLDRLGDHILERSRVATLEAIRALPKG
TFRNSMRVDGYDRPLDLVATMTISDDGIDVDFAGTSPPSSFGINVPFCYTEAYASFGVKCIIAPKIPNNEGSLALLRMRA
PADCILNAQPPLPVATRHIVGQMLPDLVIGCLGQALHGNVPAEGTSCLWNLFAFGGSSQIDADSTEMMRARVFNVMSFHS
GGTGARPGKDGLSATAFPSGVRNVPVEVTEAMSPLLIKRKEYRTDSGGPGQFRGGLGQVMEVVSLDDTAFAISANYDRVD
FPARGRDGGADGKAGKISLGSGRLLKSKGQQTIPRGEAVLIEMPGGGGLGDPFSRDAAAVAADVHLGMVSREAAETAYGV
VLRGDHSVDETATAARRGHRA

Sequences:

>Translated_581_residues
MDVHAPHADPMNVIHQQIMWDRLISVVEEQAQTLIRIGFSTSTREAGDVSAGVFNTAGHMLAQAVTGTPGHVNSMARAVV
HFLDKFPATTMQPGDIFITNDPWKGTGHLHDFTVVTPVFRADRLVALFASTCHVIDIGGRGMGPDARQVFEEGVFIPIMR
FASAAGTNETLLEIIKGNVREPVQVVGDLYSLAGCNDVGGRQLLKMMDEFGIETLDRLGDHILERSRVATLEAIRALPKG
TFRNSMRVDGYDRPLDLVATMTISDDGIDVDFAGTSPPSSFGINVPFCYTEAYASFGVKCIIAPKIPNNEGSLALLRMRA
PADCILNAQPPLPVATRHIVGQMLPDLVIGCLGQALHGNVPAEGTSCLWNLFAFGGSSQIDADSTEMMRARVFNVMSFHS
GGTGARPGKDGLSATAFPSGVRNVPVEVTEAMSPLLIKRKEYRTDSGGPGQFRGGLGQVMEVVSLDDTAFAISANYDRVD
FPARGRDGGADGKAGKISLGSGRLLKSKGQQTIPRGEAVLIEMPGGGGLGDPFSRDAAAVAADVHLGMVSREAAETAYGV
VLRGDHSVDETATAARRGHRA
>Mature_581_residues
MDVHAPHADPMNVIHQQIMWDRLISVVEEQAQTLIRIGFSTSTREAGDVSAGVFNTAGHMLAQAVTGTPGHVNSMARAVV
HFLDKFPATTMQPGDIFITNDPWKGTGHLHDFTVVTPVFRADRLVALFASTCHVIDIGGRGMGPDARQVFEEGVFIPIMR
FASAAGTNETLLEIIKGNVREPVQVVGDLYSLAGCNDVGGRQLLKMMDEFGIETLDRLGDHILERSRVATLEAIRALPKG
TFRNSMRVDGYDRPLDLVATMTISDDGIDVDFAGTSPPSSFGINVPFCYTEAYASFGVKCIIAPKIPNNEGSLALLRMRA
PADCILNAQPPLPVATRHIVGQMLPDLVIGCLGQALHGNVPAEGTSCLWNLFAFGGSSQIDADSTEMMRARVFNVMSFHS
GGTGARPGKDGLSATAFPSGVRNVPVEVTEAMSPLLIKRKEYRTDSGGPGQFRGGLGQVMEVVSLDDTAFAISANYDRVD
FPARGRDGGADGKAGKISLGSGRLLKSKGQQTIPRGEAVLIEMPGGGGLGDPFSRDAAAVAADVHLGMVSREAAETAYGV
VLRGDHSVDETATAARRGHRA

Specific function: HyuA and hyuB convert D- and L-substituted hydantoins to corresponding N-carbamyl-amino acids [H]

COG id: COG0146

COG function: function code EQ; N-methylhydantoinase B/acetone carboxylase, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

Organism=Homo sapiens, GI48314820, Length=556, Percent_Identity=26.6187050359712, Blast_Score=132, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI133901900, Length=557, Percent_Identity=26.032315978456, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI133901902, Length=539, Percent_Identity=25.7884972170686, Blast_Score=136, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6322634, Length=552, Percent_Identity=25.9057971014493, Blast_Score=126, Evalue=9e-30,
Organism=Drosophila melanogaster, GI45550492, Length=583, Percent_Identity=24.3567753001715, Blast_Score=125, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003692 [H]

Pfam domain/function: PF02538 Hydantoinase_B [H]

EC number: NA

Molecular weight: Translated: 61820; Mature: 61820

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVHAPHADPMNVIHQQIMWDRLISVVEEQAQTLIRIGFSTSTREAGDVSAGVFNTAGHM
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHH
LAQAVTGTPGHVNSMARAVVHFLDKFPATTMQPGDIFITNDPWKGTGHLHDFTVVTPVFR
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHH
ADRLVALFASTCHVIDIGGRGMGPDARQVFEEGVFIPIMRFASAAGTNETLLEIIKGNVR
HHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHCCCC
EPVQVVGDLYSLAGCNDVGGRQLLKMMDEFGIETLDRLGDHILERSRVATLEAIRALPKG
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TFRNSMRVDGYDRPLDLVATMTISDDGIDVDFAGTSPPSSFGINVPFCYTEAYASFGVKC
CCCCCCCCCCCCCCEEEEEEEEECCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHCCEEE
IIAPKIPNNEGSLALLRMRAPADCILNAQPPLPVATRHIVGQMLPDLVIGCLGQALHGNV
EEECCCCCCCCCEEEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
PAEGTSCLWNLFAFGGSSQIDADSTEMMRARVFNVMSFHSGGTGARPGKDGLSATAFPSG
CCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
VRNVPVEVTEAMSPLLIKRKEYRTDSGGPGQFRGGLGQVMEVVSLDDTAFAISANYDRVD
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCHHHHHHHHCCCCCEEEEECCCCCCC
FPARGRDGGADGKAGKISLGSGRLLKSKGQQTIPRGEAVLIEMPGGGGLGDPFSRDAAAV
CCCCCCCCCCCCCCCEEECCCCCHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHH
AADVHLGMVSREAAETAYGVVLRGDHSVDETATAARRGHRA
HHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MDVHAPHADPMNVIHQQIMWDRLISVVEEQAQTLIRIGFSTSTREAGDVSAGVFNTAGHM
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHH
LAQAVTGTPGHVNSMARAVVHFLDKFPATTMQPGDIFITNDPWKGTGHLHDFTVVTPVFR
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHH
ADRLVALFASTCHVIDIGGRGMGPDARQVFEEGVFIPIMRFASAAGTNETLLEIIKGNVR
HHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHCCCC
EPVQVVGDLYSLAGCNDVGGRQLLKMMDEFGIETLDRLGDHILERSRVATLEAIRALPKG
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TFRNSMRVDGYDRPLDLVATMTISDDGIDVDFAGTSPPSSFGINVPFCYTEAYASFGVKC
CCCCCCCCCCCCCCEEEEEEEEECCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHCCEEE
IIAPKIPNNEGSLALLRMRAPADCILNAQPPLPVATRHIVGQMLPDLVIGCLGQALHGNV
EEECCCCCCCCCEEEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
PAEGTSCLWNLFAFGGSSQIDADSTEMMRARVFNVMSFHSGGTGARPGKDGLSATAFPSG
CCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
VRNVPVEVTEAMSPLLIKRKEYRTDSGGPGQFRGGLGQVMEVVSLDDTAFAISANYDRVD
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCHHHHHHHHCCCCCEEEEECCCCCCC
FPARGRDGGADGKAGKISLGSGRLLKSKGQQTIPRGEAVLIEMPGGGGLGDPFSRDAAAV
CCCCCCCCCCCCCCCEEECCCCCHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHH
AADVHLGMVSREAAETAYGVVLRGDHSVDETATAARRGHRA
HHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732229 [H]