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The map label for this gene is trpE(G) [H]

Identifier: 86751420

GI number: 86751420

Start: 4901468

End: 4903630

Strand: Reverse

Name: trpE(G) [H]

Synonym: RPB_4318

Alternate gene names: 86751420

Gene position: 4903630-4901468 (Counterclockwise)

Preceding gene: 86751423

Following gene: 86751419

Centisome position: 91.97

GC content: 67.73

Gene sequence:

>2163_bases
ATGAACAGGACCGTTTTCTCGCTTCCCGCGCAGAGCGACTACAAGACCGCCGCGGGCCTCGCCGTGTCGCGCACGGTGGC
GGCCTTCACCGGCGGCAGGGCGCTCGATGATCTGATCGACCTTCTGGACCGCCGCCGCGGCGTGATGCTGTCGTCCGGCA
CTACGGTGCCCGGGCGCTACGAGAGCTTCGATTTCGGCTTCGCCGATCCGCCGCTGGCGCTGACCATCCGCGCCGAGCAG
TTTTCGATCGAGGCGCTCAATCCGCGCGGCCATGTGCTGGTCGCGTTCCTGTCCGACAGGCTCGACGAGCCCTGCGTGGT
GGTCGAGCAGGCCTGCGCGACGAAGATCAGGGGCCACATCGTTCGCGGCGAGGCGCCGGTCGATGAGGAGCAGCGCACCC
GCCGCGCCAGCGCGATCTCGCTGGTGCGCGCGCTGGTGGCGGCGTTCGCCTCACCGGCGGACCCGATGCTCGGCCTGTAC
GGCGCCTTCGCCTACGATCTGGTGTTCCAGTTCGAGGATCTGGCGCAGAAGCGCGCGCGCGAGGCCGACCAGCGCGACAT
CGTGCTGTATGTGCCGGATCGGCTGTTGGCCTATGACCGCGCCACCGGCCGCGGCGTCAATATCGCCTATGAATTCGCCT
GGAAGGGCAAATCCACCCAGGGCCTGCCCAACGACACCGCCGAGAGCGCCTACACCCAGACCGGCCGGCAGGGCTTCGCC
GACCACGCGCCGGGCGAATACGCCAAAGTCGTCGAGGTCGCCCGCGAGCATTTCGCCCGCGGCGATCTGTTCGAGGCGGT
GCCCGGGCAGTTGTTCGGCGAACCCTGCGAGCGCTCGCCGGCCGAAGTGTTCAAGCGGCTGTGCCGGATCAATCCGTCGC
CCTATGGCGGGTTGCTCAATCTCGGCGACGGCGAATTCCTGGTGTCGGCCTCGCCGGAGATGTTCGTCCGCTCCGACGGC
CGCCGGATCGAGACCTGCCCGATCTCCGGCACCATCGCCCGCGGCGTCGACGCCATTGCGGATGCCGAGCAGATCCAGAA
GCTTTTGAACTCCGAGAAGGACGAGTTCGAGCTCAACATGTGCACCGACGTCGACCGCAACGACAAGGCGCGGGTCTGCG
TGCCGGGCACCATCAAGGTGCTGGCGCGGCGCCAGATCGAGACCTATTCGAAGCTGTTCCACACGGTGGACCACGTCGAG
GGCATGCTCCGCCCCGGCTTCGACGCGCTCGATGCCTTCCTCACCCACGCCTGGGCGGTGACGGTGACCGGCGCGCCGAA
ACTCTGGGCGATGCAGTTCGTCGAGGATCACGAGCGCTCGCCGCGGCGCTGGTATGCCGGCGCGTTCGGCGTCGTCGGCT
TCGACGGCTCGATCAACACCGGCCTCACCATCCGTACCATCCGGATGAAGGACGGCCTCGCCGAAGTCCGCGTCGGGGCG
ACTTGTCTGTTCGATAGCGACCCGGTCGCCGAGGACAAGGAGTGCCAGGTGAAAGCCGCCGCGCTGTTCCAGGCGCTGCG
CGGCGATCCGCCGAAGCCGCTGTCGGCGGTGGCACCGGATGCCACCGGCTCCGGCAAGCGCGTGCTGCTGGTCGATCACG
ACGACAGCTTCGTGCACATGCTGGCGGACTATTTCCGCCAGGTCGGCGCCCAGGTCAGCGTGGTGCGCCACATCCACGCG
CAGAAGATGCTGGCCGAGAATGCCTATGATCTGCTGGTGCTGTCGCCCGGCCCGGGCCGGCCGGCGGATTTCAAGATTGC
GTTGACGATCGACACCGCTCTGGCGAAGCAACTGCCGATCTTCGGGGTCTGCCTCGGCGTGCAGGCGATGGGCGAATATT
TCGGCGGTACGCTCGGCCAGCTTAAGCAGCCGGCGCATGGCCGGCCGTCGCGGATCCAGGTGCGCGGCGGCACGCTGATG
CACGGCCTGCCGAACGAGATCACCATCGGTCGCTATCATTCACTCTATGTCGACATGCAGGACATGCCGGATGCGCTCGA
CGTCACCGCCTCGACCGAGGACGGCATCGCGATGGCGATCGAGCACAAGACGCTGCCGGTCGGCGGCGTCCAATTCCACC
CGGAATCGCTGATGTCGCTCGGCGGCGAGGTCGGGCTGCGGATCGTCGAAAACGCCTTCCGGCTGGGCCGGCCGATGGCC
TGA

Upstream 100 bases:

>100_bases
GATTTTCCGTTTTTTGAATCGTCCCGAGGCCGCCCCACAGTGGCGGCCTTTCGTTTGTCCTGTGTGGCGTTCCTCCGGCA
AGTTGAGATAAGAGGATGCC

Downstream 100 bases:

>100_bases
TCCTGAAATTGATCGAAACACGTGTGTCGTCGGGAGTGGGCTGCTAACACGCCCCGGCGGCAGTTATCCGAATTACCGGA
TTGCCTCCGACGCCCCGCAA

Product: anthranilate synthase

Products: NA

Alternate protein names: Glutamine amidotransferase [H]

Number of amino acids: Translated: 720; Mature: 720

Protein sequence:

>720_residues
MNRTVFSLPAQSDYKTAAGLAVSRTVAAFTGGRALDDLIDLLDRRRGVMLSSGTTVPGRYESFDFGFADPPLALTIRAEQ
FSIEALNPRGHVLVAFLSDRLDEPCVVVEQACATKIRGHIVRGEAPVDEEQRTRRASAISLVRALVAAFASPADPMLGLY
GAFAYDLVFQFEDLAQKRAREADQRDIVLYVPDRLLAYDRATGRGVNIAYEFAWKGKSTQGLPNDTAESAYTQTGRQGFA
DHAPGEYAKVVEVAREHFARGDLFEAVPGQLFGEPCERSPAEVFKRLCRINPSPYGGLLNLGDGEFLVSASPEMFVRSDG
RRIETCPISGTIARGVDAIADAEQIQKLLNSEKDEFELNMCTDVDRNDKARVCVPGTIKVLARRQIETYSKLFHTVDHVE
GMLRPGFDALDAFLTHAWAVTVTGAPKLWAMQFVEDHERSPRRWYAGAFGVVGFDGSINTGLTIRTIRMKDGLAEVRVGA
TCLFDSDPVAEDKECQVKAAALFQALRGDPPKPLSAVAPDATGSGKRVLLVDHDDSFVHMLADYFRQVGAQVSVVRHIHA
QKMLAENAYDLLVLSPGPGRPADFKIALTIDTALAKQLPIFGVCLGVQAMGEYFGGTLGQLKQPAHGRPSRIQVRGGTLM
HGLPNEITIGRYHSLYVDMQDMPDALDVTASTEDGIAMAIEHKTLPVGGVQFHPESLMSLGGEVGLRIVENAFRLGRPMA

Sequences:

>Translated_720_residues
MNRTVFSLPAQSDYKTAAGLAVSRTVAAFTGGRALDDLIDLLDRRRGVMLSSGTTVPGRYESFDFGFADPPLALTIRAEQ
FSIEALNPRGHVLVAFLSDRLDEPCVVVEQACATKIRGHIVRGEAPVDEEQRTRRASAISLVRALVAAFASPADPMLGLY
GAFAYDLVFQFEDLAQKRAREADQRDIVLYVPDRLLAYDRATGRGVNIAYEFAWKGKSTQGLPNDTAESAYTQTGRQGFA
DHAPGEYAKVVEVAREHFARGDLFEAVPGQLFGEPCERSPAEVFKRLCRINPSPYGGLLNLGDGEFLVSASPEMFVRSDG
RRIETCPISGTIARGVDAIADAEQIQKLLNSEKDEFELNMCTDVDRNDKARVCVPGTIKVLARRQIETYSKLFHTVDHVE
GMLRPGFDALDAFLTHAWAVTVTGAPKLWAMQFVEDHERSPRRWYAGAFGVVGFDGSINTGLTIRTIRMKDGLAEVRVGA
TCLFDSDPVAEDKECQVKAAALFQALRGDPPKPLSAVAPDATGSGKRVLLVDHDDSFVHMLADYFRQVGAQVSVVRHIHA
QKMLAENAYDLLVLSPGPGRPADFKIALTIDTALAKQLPIFGVCLGVQAMGEYFGGTLGQLKQPAHGRPSRIQVRGGTLM
HGLPNEITIGRYHSLYVDMQDMPDALDVTASTEDGIAMAIEHKTLPVGGVQFHPESLMSLGGEVGLRIVENAFRLGRPMA
>Mature_720_residues
MNRTVFSLPAQSDYKTAAGLAVSRTVAAFTGGRALDDLIDLLDRRRGVMLSSGTTVPGRYESFDFGFADPPLALTIRAEQ
FSIEALNPRGHVLVAFLSDRLDEPCVVVEQACATKIRGHIVRGEAPVDEEQRTRRASAISLVRALVAAFASPADPMLGLY
GAFAYDLVFQFEDLAQKRAREADQRDIVLYVPDRLLAYDRATGRGVNIAYEFAWKGKSTQGLPNDTAESAYTQTGRQGFA
DHAPGEYAKVVEVAREHFARGDLFEAVPGQLFGEPCERSPAEVFKRLCRINPSPYGGLLNLGDGEFLVSASPEMFVRSDG
RRIETCPISGTIARGVDAIADAEQIQKLLNSEKDEFELNMCTDVDRNDKARVCVPGTIKVLARRQIETYSKLFHTVDHVE
GMLRPGFDALDAFLTHAWAVTVTGAPKLWAMQFVEDHERSPRRWYAGAFGVVGFDGSINTGLTIRTIRMKDGLAEVRVGA
TCLFDSDPVAEDKECQVKAAALFQALRGDPPKPLSAVAPDATGSGKRVLLVDHDDSFVHMLADYFRQVGAQVSVVRHIHA
QKMLAENAYDLLVLSPGPGRPADFKIALTIDTALAKQLPIFGVCLGVQAMGEYFGGTLGQLKQPAHGRPSRIQVRGGTLM
HGLPNEITIGRYHSLYVDMQDMPDALDVTASTEDGIAMAIEHKTLPVGGVQFHPESLMSLGGEVGLRIVENAFRLGRPMA

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=409, Percent_Identity=31.2958435207824, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI1788114, Length=354, Percent_Identity=28.8135593220339, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1789760, Length=178, Percent_Identity=41.0112359550562, Blast_Score=122, Evalue=7e-29,
Organism=Escherichia coli, GI1787517, Length=179, Percent_Identity=40.2234636871508, Blast_Score=119, Evalue=7e-28,
Organism=Escherichia coli, GI1786809, Length=229, Percent_Identity=28.3842794759825, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6320935, Length=280, Percent_Identity=34.6428571428571, Blast_Score=156, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6322638, Length=190, Percent_Identity=38.9473684210526, Blast_Score=132, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6324361, Length=126, Percent_Identity=34.9206349206349, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR006805
- InterPro:   IPR006220
- InterPro:   IPR010112
- InterPro:   IPR015890
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR006221 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind; PF00117 GATase [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 78506; Mature: 78506

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRTVFSLPAQSDYKTAAGLAVSRTVAAFTGGRALDDLIDLLDRRRGVMLSSGTTVPGRY
CCCCEEECCCCCCCHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEECCCCCCCCC
ESFDFGFADPPLALTIRAEQFSIEALNPRGHVLVAFLSDRLDEPCVVVEQACATKIRGHI
CCCCCCCCCCCEEEEEEECEEEEEEECCCCCEEEEEEHHCCCCCHHHHHHHHHHHHHCEE
VRGEAPVDEEQRTRRASAISLVRALVAAFASPADPMLGLYGAFAYDLVFQFEDLAQKRAR
EECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EADQRDIVLYVPDRLLAYDRATGRGVNIAYEFAWKGKSTQGLPNDTAESAYTQTGRQGFA
CCCCCCEEEEECCHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCC
DHAPGEYAKVVEVAREHFARGDLFEAVPGQLFGEPCERSPAEVFKRLCRINPSPYGGLLN
CCCCCHHHHHHHHHHHHHCCCCHHHHCCHHHCCCCCCCCHHHHHHHHHCCCCCCCCCEEE
LGDGEFLVSASPEMFVRSDGRRIETCPISGTIARGVDAIADAEQIQKLLNSEKDEFELNM
CCCCCEEEECCCCEEEECCCCEEEECCCCCHHHHHHHHHCCHHHHHHHHCCCCCCEEEEE
CTDVDRNDKARVCVPGTIKVLARRQIETYSKLFHTVDHVEGMLRPGFDALDAFLTHAWAV
ECCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCEEEE
TVTGAPKLWAMQFVEDHERSPRRWYAGAFGVVGFDGSINTGLTIRTIRMKDGLAEVRVGA
EECCCCHHHHHHHHHHHCCCCCCEECCCCEEEEECCCCCCCEEEEEEEECCCHHHHEECE
TCLFDSDPVAEDKECQVKAAALFQALRGDPPKPLSAVAPDATGSGKRVLLVDHDDSFVHM
EEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCCEEEEEECCCHHHHH
LADYFRQVGAQVSVVRHIHAQKMLAENAYDLLVLSPGPGRPADFKIALTIDTALAKQLPI
HHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEECHHHHHHCCH
FGVCLGVQAMGEYFGGTLGQLKQPAHGRPSRIQVRGGTLMHGLPNEITIGRYHSLYVDMQ
HHHHHHHHHHHHHHCCCHHHHCCCCCCCCCEEEEECCEEECCCCCCEEEEEEEEEEEEHH
DMPDALDVTASTEDGIAMAIEHKTLPVGGVQFHPESLMSLGGEVGLRIVENAFRLGRPMA
HCCCCCEEECCCCCCEEEEEECCCCCCCCEEECHHHHHHCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNRTVFSLPAQSDYKTAAGLAVSRTVAAFTGGRALDDLIDLLDRRRGVMLSSGTTVPGRY
CCCCEEECCCCCCCHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEECCCCCCCCC
ESFDFGFADPPLALTIRAEQFSIEALNPRGHVLVAFLSDRLDEPCVVVEQACATKIRGHI
CCCCCCCCCCCEEEEEEECEEEEEEECCCCCEEEEEEHHCCCCCHHHHHHHHHHHHHCEE
VRGEAPVDEEQRTRRASAISLVRALVAAFASPADPMLGLYGAFAYDLVFQFEDLAQKRAR
EECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EADQRDIVLYVPDRLLAYDRATGRGVNIAYEFAWKGKSTQGLPNDTAESAYTQTGRQGFA
CCCCCCEEEEECCHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCC
DHAPGEYAKVVEVAREHFARGDLFEAVPGQLFGEPCERSPAEVFKRLCRINPSPYGGLLN
CCCCCHHHHHHHHHHHHHCCCCHHHHCCHHHCCCCCCCCHHHHHHHHHCCCCCCCCCEEE
LGDGEFLVSASPEMFVRSDGRRIETCPISGTIARGVDAIADAEQIQKLLNSEKDEFELNM
CCCCCEEEECCCCEEEECCCCEEEECCCCCHHHHHHHHHCCHHHHHHHHCCCCCCEEEEE
CTDVDRNDKARVCVPGTIKVLARRQIETYSKLFHTVDHVEGMLRPGFDALDAFLTHAWAV
ECCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCEEEE
TVTGAPKLWAMQFVEDHERSPRRWYAGAFGVVGFDGSINTGLTIRTIRMKDGLAEVRVGA
EECCCCHHHHHHHHHHHCCCCCCEECCCCEEEEECCCCCCCEEEEEEEECCCHHHHEECE
TCLFDSDPVAEDKECQVKAAALFQALRGDPPKPLSAVAPDATGSGKRVLLVDHDDSFVHM
EEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCCEEEEEECCCHHHHH
LADYFRQVGAQVSVVRHIHAQKMLAENAYDLLVLSPGPGRPADFKIALTIDTALAKQLPI
HHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEECHHHHHHCCH
FGVCLGVQAMGEYFGGTLGQLKQPAHGRPSRIQVRGGTLMHGLPNEITIGRYHSLYVDMQ
HHHHHHHHHHHHHHCCCHHHHCCCCCCCCCEEEEECCEEECCCCCCEEEEEEEEEEEEHH
DMPDALDVTASTEDGIAMAIEHKTLPVGGVQFHPESLMSLGGEVGLRIVENAFRLGRPMA
HCCCCCEEECCCCCCEEEEEECCCCCCCCEEECHHHHHHCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8939798 [H]