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The map label for this gene is sigW [H]

Identifier: 86751382

GI number: 86751382

Start: 4861396

End: 4862115

Strand: Reverse

Name: sigW [H]

Synonym: RPB_4279

Alternate gene names: 86751382

Gene position: 4862115-4861396 (Counterclockwise)

Preceding gene: 86751390

Following gene: 86751381

Centisome position: 91.19

GC content: 70.0

Gene sequence:

>720_bases
ATGTCGGTCCGCGGGTGGTGGTGGCCGGGCCGCCGGTGGTGGTCGCGCCGCGCAGCTGCTGGATCGCGGGCGCCTGGCGT
CCCTGCTGAACCGATGCGGGGGAGCCCGGCCGTCGCGCGCGCGCGAATGGTCGAGATGTTGGCCGGGCCGGGGGCGGCAC
TGATGGACGATGACAGCGACGAGGCGTTGATGGCCGCCGTGGTTGCGCGGCGGCAGCAGGCGTTTCGCGTGCTGATGAAT
CGCCACATGCCGCGCGCCATCCGGGTCGCTCAGCGCGTCGTCCGCGATCCGGCGGAGGCCGACGATATCGGCCAGGAGGC
GTTTCTGCGGGTCTGGAACAAGGCCTCGTCGTTCGATCCGCAGATCGCGCGGTTCACCACCTGGCTGTACCGCATCGTGC
TCAATCTGGCGTTCGATCGCGCGCGAAAGCCGCAGCTGGCGCCGATCGAGGAGGCCGCCGAGATCGAATCCGGCGAGGCC
GGTCCGGTCGAGACGATCATCGCCGCCCAGCAGCGTCGGCTTCTGGAACGGGCGATGGCGGCGCTGCCGGAGCGCCAGCG
CGGCGCGGTGGCGCTGTTCCACATGGAAGGTTTGAGCGGCGAGGACGCGGCGCGGGCGATGAATCTGAGCCCGAAGGCAT
TCGAATCGCTGCTGGCGCGGGGACGGGCTGCGCTGCGTAAAGAGGTCGAGAGACTAGAAGGTCACAGGAGGGACGCATGA

Upstream 100 bases:

>100_bases
GGCTGGCGTGGCCCGGGCGTCTATCCGGGATGCCGCGCCTATCGGCCGGGCTATCCGGTGCGGCCGTATCGCTATTACGC
GCGGCCGGTGGCGCCGTATT

Downstream 100 bases:

>100_bases
ATCGCGAACAATTCAGGCAACTGGCCGAAAGCTGGGGCGGTGACATCGGGCGTTGGCCGCCCGATCTCCGCGATGCCGCC
CGGCGACTGGCCGAACACGA

Product: RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-W factor [H]

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MSVRGWWWPGRRWWSRRAAAGSRAPGVPAEPMRGSPAVARARMVEMLAGPGAALMDDDSDEALMAAVVARRQQAFRVLMN
RHMPRAIRVAQRVVRDPAEADDIGQEAFLRVWNKASSFDPQIARFTTWLYRIVLNLAFDRARKPQLAPIEEAAEIESGEA
GPVETIIAAQQRRLLERAMAALPERQRGAVALFHMEGLSGEDAARAMNLSPKAFESLLARGRAALRKEVERLEGHRRDA

Sequences:

>Translated_239_residues
MSVRGWWWPGRRWWSRRAAAGSRAPGVPAEPMRGSPAVARARMVEMLAGPGAALMDDDSDEALMAAVVARRQQAFRVLMN
RHMPRAIRVAQRVVRDPAEADDIGQEAFLRVWNKASSFDPQIARFTTWLYRIVLNLAFDRARKPQLAPIEEAAEIESGEA
GPVETIIAAQQRRLLERAMAALPERQRGAVALFHMEGLSGEDAARAMNLSPKAFESLLARGRAALRKEVERLEGHRRDA
>Mature_238_residues
SVRGWWWPGRRWWSRRAAAGSRAPGVPAEPMRGSPAVARARMVEMLAGPGAALMDDDSDEALMAAVVARRQQAFRVLMNR
HMPRAIRVAQRVVRDPAEADDIGQEAFLRVWNKASSFDPQIARFTTWLYRIVLNLAFDRARKPQLAPIEEAAEIESGEAG
PVETIIAAQQRRLLERAMAALPERQRGAVALFHMEGLSGEDAARAMNLSPKAFESLLARGRAALRKEVERLEGHRRDA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Sigma-W controls genes involved in transport processes and detoxification [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=185, Percent_Identity=30.2702702702703, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014294
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 26668; Mature: 26537

Theoretical pI: Translated: 11.45; Mature: 11.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVRGWWWPGRRWWSRRAAAGSRAPGVPAEPMRGSPAVARARMVEMLAGPGAALMDDDSD
CCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCH
EALMAAVVARRQQAFRVLMNRHMPRAIRVAQRVVRDPAEADDIGQEAFLRVWNKASSFDP
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCH
QIARFTTWLYRIVLNLAFDRARKPQLAPIEEAAEIESGEAGPVETIIAAQQRRLLERAMA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
ALPERQRGAVALFHMEGLSGEDAARAMNLSPKAFESLLARGRAALRKEVERLEGHRRDA
HCCCCCCCCEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SVRGWWWPGRRWWSRRAAAGSRAPGVPAEPMRGSPAVARARMVEMLAGPGAALMDDDSD
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCH
EALMAAVVARRQQAFRVLMNRHMPRAIRVAQRVVRDPAEADDIGQEAFLRVWNKASSFDP
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCH
QIARFTTWLYRIVLNLAFDRARKPQLAPIEEAAEIESGEAGPVETIIAAQQRRLLERAMA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
ALPERQRGAVALFHMEGLSGEDAARAMNLSPKAFESLLARGRAALRKEVERLEGHRRDA
HCCCCCCCCEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274029; 9384377; 12207695 [H]