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The map label for this gene is ruvB [H]

Identifier: 86751372

GI number: 86751372

Start: 4853385

End: 4854431

Strand: Reverse

Name: ruvB [H]

Synonym: RPB_4269

Alternate gene names: 86751372

Gene position: 4854431-4853385 (Counterclockwise)

Preceding gene: 86751373

Following gene: 86751371

Centisome position: 91.05

GC content: 69.34

Gene sequence:

>1047_bases
ATGACTGACCCCTCCCGCCTCGTCACCCCCGAGCGCCGCGGCGATGATCTCGGCGATGCGGCGCTGCGGCCGCAGAATCT
GTCCGAATTCGTCGGGCAGGAGAAGGCGCGCGCCAATCTGCAGGTGTTCATCGACGCCGCCCGCAAGCGCAAGGAGGCGC
TCGACCACGTGCTGTTCGTCGGGCCCCCCGGCCTCGGCAAGACCACGCTGGCGCAGATCGTCGCCCGCGAACTCGGCGTC
GGGTTTCGCGCCACCTCGGGCCCGGTGATCGCCAAGGCCGGCGATCTCGCAGCCTTGCTGACCAATCTCGAAGAGCGCGA
CGTGCTGTTCATCGACGAGATCCACCGCCTCAGCCCGGCGGTGGAAGAAGTGCTGTATCCGGCGATGGAGGATTTTCAGC
TCGATCTGATCATCGGCGAAGGTCCGGCGGCGCGCTCGGTCAAGATCGAGCTGTCGAAATTCACCCTGGTCGGCGCCACC
ACGCGCGCGGGCCTGCTGACCAATCCGCTGCGCGACCGCTTCGGCATTCCGGTGCGGCTGAACTTCTACACGATTGAAGA
GCTGGAAAGCATCGTCAGCCGCGGCGCCCGCGTGCTCGGCACCGGTATCACGGCGGACGGCGCCAACGAGATCGCCCGCC
GCGCCCGCGGCACGCCGCGCATCGCCGGCCGGCTGCTGCGCCGGGTCCGCGATTTCGCCTCGGCAGCCGATGCCGAAGCG
ATCGACCGCAAGATCGCCGACCACGCGCTCGGCGCGCTCGAAGTCGACGCCGCCGGCCTCGACGCGATGGACCGGCGTTA
CTTGTCGACGATCGCGCTGAACTATGGCGGCGGCCCGGTCGGCGTCGAGACCATGGCGGCGGCGCTGTCGGAGCCGCGCG
ACGCCATCGAGGACATCATCGAGCCCTATCTGATCCAGTGCGGCTATCTGCAGCGCACCCCGCGCGGCCGCCTCTTGACG
CCGCACGCCTTCAAGCACCTCGGCCTCGCCGAGCCTTCGCGCGAGCCCGGGCAGTTCGGGCTGTTCGGCGGGGAGGAAGA
GGCGTAA

Upstream 100 bases:

>100_bases
CCCACCCCCGACCCCGGATCAAGTCCGGGGCAGGCTCCCTCCCCGCAAGGGGGAGGGGAGAAGAGGGCGCAGTGCACACT
CACCATGCTATCCTCGCGCC

Downstream 100 bases:

>100_bases
CGACACTCGCATCGGCGCCAAAATCAGCTATATTCGGCTGATGAGCTCCGTGATCGAAAAAGCCGTCGAAGCCTTGGAGA
TGATGCCGGACGACATGCGT

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MTDPSRLVTPERRGDDLGDAALRPQNLSEFVGQEKARANLQVFIDAARKRKEALDHVLFVGPPGLGKTTLAQIVARELGV
GFRATSGPVIAKAGDLAALLTNLEERDVLFIDEIHRLSPAVEEVLYPAMEDFQLDLIIGEGPAARSVKIELSKFTLVGAT
TRAGLLTNPLRDRFGIPVRLNFYTIEELESIVSRGARVLGTGITADGANEIARRARGTPRIAGRLLRRVRDFASAADAEA
IDRKIADHALGALEVDAAGLDAMDRRYLSTIALNYGGGPVGVETMAAALSEPRDAIEDIIEPYLIQCGYLQRTPRGRLLT
PHAFKHLGLAEPSREPGQFGLFGGEEEA

Sequences:

>Translated_348_residues
MTDPSRLVTPERRGDDLGDAALRPQNLSEFVGQEKARANLQVFIDAARKRKEALDHVLFVGPPGLGKTTLAQIVARELGV
GFRATSGPVIAKAGDLAALLTNLEERDVLFIDEIHRLSPAVEEVLYPAMEDFQLDLIIGEGPAARSVKIELSKFTLVGAT
TRAGLLTNPLRDRFGIPVRLNFYTIEELESIVSRGARVLGTGITADGANEIARRARGTPRIAGRLLRRVRDFASAADAEA
IDRKIADHALGALEVDAAGLDAMDRRYLSTIALNYGGGPVGVETMAAALSEPRDAIEDIIEPYLIQCGYLQRTPRGRLLT
PHAFKHLGLAEPSREPGQFGLFGGEEEA
>Mature_347_residues
TDPSRLVTPERRGDDLGDAALRPQNLSEFVGQEKARANLQVFIDAARKRKEALDHVLFVGPPGLGKTTLAQIVARELGVG
FRATSGPVIAKAGDLAALLTNLEERDVLFIDEIHRLSPAVEEVLYPAMEDFQLDLIIGEGPAARSVKIELSKFTLVGATT
RAGLLTNPLRDRFGIPVRLNFYTIEELESIVSRGARVLGTGITADGANEIARRARGTPRIAGRLLRRVRDFASAADAEAI
DRKIADHALGALEVDAAGLDAMDRRYLSTIALNYGGGPVGVETMAAALSEPRDAIEDIIEPYLIQCGYLQRTPRGRLLTP
HAFKHLGLAEPSREPGQFGLFGGEEEA

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family [H]

Homologues:

Organism=Escherichia coli, GI1788167, Length=318, Percent_Identity=62.8930817610063, Blast_Score=404, Evalue=1e-114,
Organism=Escherichia coli, GI1787119, Length=126, Percent_Identity=33.3333333333333, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- ProDom:   PD005323 [H]

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 37619; Mature: 37488

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDPSRLVTPERRGDDLGDAALRPQNLSEFVGQEKARANLQVFIDAARKRKEALDHVLFV
CCCCCCCCCCCCCCCCCCCHHCCCCCHHHHHCHHHHHCCHHHHHHHHHHHHHHHHHEEEE
GPPGLGKTTLAQIVARELGVGFRATSGPVIAKAGDLAALLTNLEERDVLFIDEIHRLSPA
CCCCCCHHHHHHHHHHHHCCCEECCCCCEEECCCHHHHHHHCCCCCCEEHHHHHHHHHHH
VEEVLYPAMEDFQLDLIIGEGPAARSVKIELSKFTLVGATTRAGLLTNPLRDRFGIPVRL
HHHHHHHHHHCCEEEEEECCCCCCCEEEEEEEEEEEEECCHHCCHHHCCHHHHCCCCEEE
NFYTIEELESIVSRGARVLGTGITADGANEIARRARGTPRIAGRLLRRVRDFASAADAEA
EEEEHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHH
IDRKIADHALGALEVDAAGLDAMDRRYLSTIALNYGGGPVGVETMAAALSEPRDAIEDII
HHHHHHHHHCCCEEECCCCCCHHHHHHHHHHEEECCCCCCCHHHHHHHHHCHHHHHHHHH
EPYLIQCGYLQRTPRGRLLTPHAFKHLGLAEPSREPGQFGLFGGEEEA
HHHHHHCCCCCCCCCCCEECHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDPSRLVTPERRGDDLGDAALRPQNLSEFVGQEKARANLQVFIDAARKRKEALDHVLFV
CCCCCCCCCCCCCCCCCCHHCCCCCHHHHHCHHHHHCCHHHHHHHHHHHHHHHHHEEEE
GPPGLGKTTLAQIVARELGVGFRATSGPVIAKAGDLAALLTNLEERDVLFIDEIHRLSPA
CCCCCCHHHHHHHHHHHHCCCEECCCCCEEECCCHHHHHHHCCCCCCEEHHHHHHHHHHH
VEEVLYPAMEDFQLDLIIGEGPAARSVKIELSKFTLVGATTRAGLLTNPLRDRFGIPVRL
HHHHHHHHHHCCEEEEEECCCCCCCEEEEEEEEEEEEECCHHCCHHHCCHHHHCCCCEEE
NFYTIEELESIVSRGARVLGTGITADGANEIARRARGTPRIAGRLLRRVRDFASAADAEA
EEEEHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHH
IDRKIADHALGALEVDAAGLDAMDRRYLSTIALNYGGGPVGVETMAAALSEPRDAIEDII
HHHHHHHHHCCCEEECCCCCCHHHHHHHHHHEEECCCCCCCHHHHHHHHHCHHHHHHHHH
EPYLIQCGYLQRTPRGRLLTPHAFKHLGLAEPSREPGQFGLFGGEEEA
HHHHHHCCCCCCCCCCCEECHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA