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The map label for this gene is gyaR [H]

Identifier: 86751332

GI number: 86751332

Start: 4802195

End: 4803190

Strand: Reverse

Name: gyaR [H]

Synonym: RPB_4226

Alternate gene names: 86751332

Gene position: 4803190-4802195 (Counterclockwise)

Preceding gene: 86751333

Following gene: 86751330

Centisome position: 90.09

GC content: 67.27

Gene sequence:

>996_bases
GTGAAAGTCGCCATTCTCGACGATTACTTCGACACGCTGCGCACGCTGAACTGCTTCGGCCGGCTGCAAGGCCACGACGT
CACGGTGTTCAACGATCACGTCCAGGACACCGATGCGCTGGCTTCGCGGCTGCGCGACACCGAGGCGCTGGTGCTGATCC
GCGAGCGGACGCAGATTCGTGCCGCCCTGCTGGAGAAGCTGCCGCGGTTGAAGCTGATCAGCCAGCGCGGCGTCTATCCC
CACATCGATGTCGACGCCTGCACGCGGCTCGGAATCGTCGTGTCGTCGAACATGAGCGCCGGCGCGCCGTCCTATGCGGC
GGCGGAATTGACCTGGGGCCTGGTGCTGGGGGCGATGCGGCAGATCCCGCAGCAGATGGCGGCGCTGAAGGCCGGCGTCT
GGCAGATCGGCGTCGGTCACACGCTGCGTGACAAGACGCTCGGCATCTACGGCTACGGCCGGATCGGCCGCGTCGTGGCG
GGCTACGGCCGCGCCTTCGGCATGACCGTGCTGGTCTGGGCGCGCGAGCCCAATCTCGCCGAGGCGCGCGCCGACGGTTA
TCAGATCGCCGGCAGCAAGGAAGACTTGTTTGCCCACAGTGACGTGCTGTCGCTGCACATGCGCTTGATCGACGCCACCC
GCGGCATCGTCACGCGCGCGGATCTGGCGCGGATGAAGCCGACGGCGCTGCTGGTCAACACCAGCCGCGCCGGACTGATC
GAGCAGGGGGCCCTCGTCGCGGCGCTCCGCGCCGGGCGTCCCGGCATGGCGGCGATCGATGTGTTCGACACCGAGCCGCT
GCGCGATCCGCAGGATCCGCTACTGGCGATGGACAACGTCGTTGCCACGCCGCATATCGGCTACGTGTCGCGTGACGAAT
ACGAGCTGCAATTCGGCGATATCTTCGAGCAGATCGTCGCCTATGCGGCGGGCGAGCCGATCAATGTGGTCAACCCCGCA
TCACTGTCCTCGTCGCGGTCCTCGTCGCGGCGCTGA

Upstream 100 bases:

>100_bases
AAGGACGGCTGCGCGCTGGGCTACTGAGGGTCGAGGCCAGCGCCGCTGGCCTCGCTCCTTCCACCACGAGAATAACCACC
GCAACGGGAGCTTGCGCCGC

Downstream 100 bases:

>100_bases
TCCCGGCTTCCAAACGCAGAAAGACCGGCCGGAGGCCGGTCTTTCGTTGTGATCGCGGGCGGGTAAACGTCACGCGATCA
ATCAGGTCGCGCTCAGAGGT

Product: D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MKVAILDDYFDTLRTLNCFGRLQGHDVTVFNDHVQDTDALASRLRDTEALVLIRERTQIRAALLEKLPRLKLISQRGVYP
HIDVDACTRLGIVVSSNMSAGAPSYAAAELTWGLVLGAMRQIPQQMAALKAGVWQIGVGHTLRDKTLGIYGYGRIGRVVA
GYGRAFGMTVLVWAREPNLAEARADGYQIAGSKEDLFAHSDVLSLHMRLIDATRGIVTRADLARMKPTALLVNTSRAGLI
EQGALVAALRAGRPGMAAIDVFDTEPLRDPQDPLLAMDNVVATPHIGYVSRDEYELQFGDIFEQIVAYAAGEPINVVNPA
SLSSSRSSSRR

Sequences:

>Translated_331_residues
MKVAILDDYFDTLRTLNCFGRLQGHDVTVFNDHVQDTDALASRLRDTEALVLIRERTQIRAALLEKLPRLKLISQRGVYP
HIDVDACTRLGIVVSSNMSAGAPSYAAAELTWGLVLGAMRQIPQQMAALKAGVWQIGVGHTLRDKTLGIYGYGRIGRVVA
GYGRAFGMTVLVWAREPNLAEARADGYQIAGSKEDLFAHSDVLSLHMRLIDATRGIVTRADLARMKPTALLVNTSRAGLI
EQGALVAALRAGRPGMAAIDVFDTEPLRDPQDPLLAMDNVVATPHIGYVSRDEYELQFGDIFEQIVAYAAGEPINVVNPA
SLSSSRSSSRR
>Mature_331_residues
MKVAILDDYFDTLRTLNCFGRLQGHDVTVFNDHVQDTDALASRLRDTEALVLIRERTQIRAALLEKLPRLKLISQRGVYP
HIDVDACTRLGIVVSSNMSAGAPSYAAAELTWGLVLGAMRQIPQQMAALKAGVWQIGVGHTLRDKTLGIYGYGRIGRVVA
GYGRAFGMTVLVWAREPNLAEARADGYQIAGSKEDLFAHSDVLSLHMRLIDATRGIVTRADLARMKPTALLVNTSRAGLI
EQGALVAALRAGRPGMAAIDVFDTEPLRDPQDPLLAMDNVVATPHIGYVSRDEYELQFGDIFEQIVAYAAGEPINVVNPA
SLSSSRSSSRR

Specific function: Unknown

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GyaR subfamily [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=332, Percent_Identity=30.421686746988, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI4557497, Length=244, Percent_Identity=33.6065573770492, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI61743967, Length=244, Percent_Identity=33.6065573770492, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI145580578, Length=243, Percent_Identity=32.5102880658436, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI4557499, Length=243, Percent_Identity=32.5102880658436, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI145580575, Length=243, Percent_Identity=32.5102880658436, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI6912396, Length=225, Percent_Identity=32.4444444444444, Blast_Score=98, Evalue=9e-21,
Organism=Escherichia coli, GI87082289, Length=281, Percent_Identity=33.4519572953737, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1789279, Length=318, Percent_Identity=30.5031446540881, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1787645, Length=211, Percent_Identity=29.8578199052133, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17532191, Length=325, Percent_Identity=33.2307692307692, Blast_Score=130, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI25147481, Length=270, Percent_Identity=30.3703703703704, Blast_Score=104, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6320925, Length=301, Percent_Identity=26.578073089701, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6322116, Length=279, Percent_Identity=26.8817204301075, Blast_Score=92, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6324055, Length=219, Percent_Identity=30.1369863013699, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6324964, Length=296, Percent_Identity=28.0405405405405, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19921140, Length=303, Percent_Identity=30.03300330033, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI28574286, Length=280, Percent_Identity=30.3571428571429, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24646446, Length=248, Percent_Identity=31.8548387096774, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24646448, Length=248, Percent_Identity=31.8548387096774, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24646452, Length=248, Percent_Identity=31.8548387096774, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24646450, Length=248, Percent_Identity=31.8548387096774, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI62472511, Length=248, Percent_Identity=31.8548387096774, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI45552429, Length=231, Percent_Identity=30.3030303030303, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24585514, Length=231, Percent_Identity=30.3030303030303, Blast_Score=98, Evalue=8e-21,
Organism=Drosophila melanogaster, GI28574282, Length=231, Percent_Identity=30.3030303030303, Blast_Score=98, Evalue=8e-21,
Organism=Drosophila melanogaster, GI45551003, Length=231, Percent_Identity=30.3030303030303, Blast_Score=98, Evalue=8e-21,
Organism=Drosophila melanogaster, GI28574284, Length=231, Percent_Identity=30.3030303030303, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI28571528, Length=232, Percent_Identity=31.0344827586207, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24585516, Length=214, Percent_Identity=27.5700934579439, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.26 [H]

Molecular weight: Translated: 36081; Mature: 36081

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAILDDYFDTLRTLNCFGRLQGHDVTVFNDHVQDTDALASRLRDTEALVLIRERTQIR
CEEEEEHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCCEEEEEHHHHHHH
AALLEKLPRLKLISQRGVYPHIDVDACTRLGIVVSSNMSAGAPSYAAAELTWGLVLGAMR
HHHHHHCCHHHHHHHCCCCCCCCHHHHHHEEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
QIPQQMAALKAGVWQIGVGHTLRDKTLGIYGYGRIGRVVAGYGRAFGMTVLVWAREPNLA
HHHHHHHHHHCCCEEECCCCEECCCEEEEEECCHHHHHHHHCCCCCCEEEEEEECCCCHH
EARADGYQIAGSKEDLFAHSDVLSLHMRLIDATRGIVTRADLARMKPTALLVNTSRAGLI
HHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEECCCCCCH
EQGALVAALRAGRPGMAAIDVFDTEPLRDPQDPLLAMDNVVATPHIGYVSRDEYELQFGD
HCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHCCEECCCCCCCCCCCCEEEHHHH
IFEQIVAYAAGEPINVVNPASLSSSRSSSRR
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKVAILDDYFDTLRTLNCFGRLQGHDVTVFNDHVQDTDALASRLRDTEALVLIRERTQIR
CEEEEEHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCCEEEEEHHHHHHH
AALLEKLPRLKLISQRGVYPHIDVDACTRLGIVVSSNMSAGAPSYAAAELTWGLVLGAMR
HHHHHHCCHHHHHHHCCCCCCCCHHHHHHEEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
QIPQQMAALKAGVWQIGVGHTLRDKTLGIYGYGRIGRVVAGYGRAFGMTVLVWAREPNLA
HHHHHHHHHHCCCEEECCCCEECCCEEEEEECCHHHHHHHHCCCCCCEEEEEEECCCCHH
EARADGYQIAGSKEDLFAHSDVLSLHMRLIDATRGIVTRADLARMKPTALLVNTSRAGLI
HHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEECCCCCCH
EQGALVAALRAGRPGMAAIDVFDTEPLRDPQDPLLAMDNVVATPHIGYVSRDEYELQFGD
HCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHCCEECCCCCCCCCCCCEEEHHHH
IFEQIVAYAAGEPINVVNPASLSSSRSSSRR
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]