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The map label for this gene is ilvE [H]

Identifier: 86751282

GI number: 86751282

Start: 4746044

End: 4747159

Strand: Direct

Name: ilvE [H]

Synonym: RPB_4175

Alternate gene names: 86751282

Gene position: 4746044-4747159 (Clockwise)

Preceding gene: 86751278

Following gene: 86751290

Centisome position: 89.02

GC content: 68.28

Gene sequence:

>1116_bases
ATGGGACTCTCGTTCGACAACAAGGTCGACGGCTCGGTCTGGCTGAATTTCGACATTCAGCCGTCGGCGCATCCAGTGTC
GGAGACCGAGCGGACCGCGAAGCTCGCGGACCCGGGCTTCGGCCGCGTCTTCACCGACCACATGGCGATCGTCCGCTACG
ATCAGGCCAAGGGCTGGTACGGCGCGCGCGTCGAGTCCCGCGCCAATTTCCCGCTCGATCCGGCGACCGCCGTGCTGCAC
TACGCGCAGGAGATCTTCGAGGGGCTGAAGGCCTACAAGCGTGCCGATGGCGGCGTGAACCTGTTCCGCCCCGACGCCAA
TGCGCGGCGCTTCCGCGATTCCGCCGACCGCATGGCGATGGCGCAGCTTCCCGAGCCGGTGTTCATCGAGGCGATCGAAC
AGCTGGTCCGGATCGACCGCGCGTGGATCCCGGGCGGCGACGGCAGCCTGTATCTGCGGCCGTTCATGATCGCCAGCGAG
GTGTTCCTCGGCGTCAAGCCGTCGGCCGAATACATCTTCGCGGTGATCGCCTCGCCGGTCGGCTCCTACTTCAAGGGCGG
CCCCGCGCCGGTGTCGATCTGGGTGTCGGAGAACTACACCCGCGCCGCGATCGGCGGCACCGGCAGCGTCAAATGCGGCG
GCAACTACGCGGCGTCGCTGCGCGCCCAGGCCGAGGCGATCGCGCATGGCTGCGATCAGGTGGTGTTCCTCGACGCGATC
GAGCGCCGCTATATCGAGGAACTCGGCGGCATGAACGTGTTCTTCGTGTTCGACGACGGCTCGCTGTCGACGCCGCCGCT
CGGCACCATCCTGCCCGGCATCACCCGCGATTCGATCATCGCGCTGGCGCGGCAGGCCGGCCGCACCGTGCGCGAGGAAG
CCTACACGATCGAGCAATGGCGCGCCGATGCGGCCAGCGGCAAGCTGAAAGAGGCGTTCGCCTGCGGCACCGCGGCGGTG
ATCTCGCCGATCGGCACCGTGCGCTCGGCGAGCGGCGACTTCACCATCAACGGCGGCGTCGCCGGCGAGGTGGCGATGGG
CCTGCGCAAGCAGCTCGTCGACATCCAATACGGCCGCGCCGAGGACAAGCACGGCTGGATCAGGGACGTGGCGTAA

Upstream 100 bases:

>100_bases
CGGCACGCGGCCGGGGCGCAGCACCATCCAGCGGCCGGCGACAGCCGGGCGCGCACAATGACGGCCACCGGCAGGCGCGC
CGATCCAGGAGATCACGTCC

Downstream 100 bases:

>100_bases
GCCTTTGCCGCTTTGCTTCTGAATCGTCATCCTGAGGTGCTCGCCCGCAAGGGCGAGCCTCGAAGGATGCGGACGCTCAG
TACGCGTGTCCATCCTTCGA

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MGLSFDNKVDGSVWLNFDIQPSAHPVSETERTAKLADPGFGRVFTDHMAIVRYDQAKGWYGARVESRANFPLDPATAVLH
YAQEIFEGLKAYKRADGGVNLFRPDANARRFRDSADRMAMAQLPEPVFIEAIEQLVRIDRAWIPGGDGSLYLRPFMIASE
VFLGVKPSAEYIFAVIASPVGSYFKGGPAPVSIWVSENYTRAAIGGTGSVKCGGNYAASLRAQAEAIAHGCDQVVFLDAI
ERRYIEELGGMNVFFVFDDGSLSTPPLGTILPGITRDSIIALARQAGRTVREEAYTIEQWRADAASGKLKEAFACGTAAV
ISPIGTVRSASGDFTINGGVAGEVAMGLRKQLVDIQYGRAEDKHGWIRDVA

Sequences:

>Translated_371_residues
MGLSFDNKVDGSVWLNFDIQPSAHPVSETERTAKLADPGFGRVFTDHMAIVRYDQAKGWYGARVESRANFPLDPATAVLH
YAQEIFEGLKAYKRADGGVNLFRPDANARRFRDSADRMAMAQLPEPVFIEAIEQLVRIDRAWIPGGDGSLYLRPFMIASE
VFLGVKPSAEYIFAVIASPVGSYFKGGPAPVSIWVSENYTRAAIGGTGSVKCGGNYAASLRAQAEAIAHGCDQVVFLDAI
ERRYIEELGGMNVFFVFDDGSLSTPPLGTILPGITRDSIIALARQAGRTVREEAYTIEQWRADAASGKLKEAFACGTAAV
ISPIGTVRSASGDFTINGGVAGEVAMGLRKQLVDIQYGRAEDKHGWIRDVA
>Mature_370_residues
GLSFDNKVDGSVWLNFDIQPSAHPVSETERTAKLADPGFGRVFTDHMAIVRYDQAKGWYGARVESRANFPLDPATAVLHY
AQEIFEGLKAYKRADGGVNLFRPDANARRFRDSADRMAMAQLPEPVFIEAIEQLVRIDRAWIPGGDGSLYLRPFMIASEV
FLGVKPSAEYIFAVIASPVGSYFKGGPAPVSIWVSENYTRAAIGGTGSVKCGGNYAASLRAQAEAIAHGCDQVVFLDAIE
RRYIEELGGMNVFFVFDDGSLSTPPLGTILPGITRDSIIALARQAGRTVREEAYTIEQWRADAASGKLKEAFACGTAAVI
SPIGTVRSASGDFTINGGVAGEVAMGLRKQLVDIQYGRAEDKHGWIRDVA

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI296010906, Length=331, Percent_Identity=40.785498489426, Blast_Score=238, Evalue=5e-63,
Organism=Homo sapiens, GI38176287, Length=331, Percent_Identity=40.785498489426, Blast_Score=238, Evalue=6e-63,
Organism=Homo sapiens, GI296010904, Length=331, Percent_Identity=40.785498489426, Blast_Score=238, Evalue=7e-63,
Organism=Homo sapiens, GI50658084, Length=356, Percent_Identity=37.3595505617978, Blast_Score=222, Evalue=4e-58,
Organism=Homo sapiens, GI296010900, Length=331, Percent_Identity=36.5558912386707, Blast_Score=195, Evalue=7e-50,
Organism=Homo sapiens, GI296010902, Length=331, Percent_Identity=36.5558912386707, Blast_Score=194, Evalue=9e-50,
Organism=Homo sapiens, GI258614015, Length=294, Percent_Identity=37.0748299319728, Blast_Score=179, Evalue=3e-45,
Organism=Escherichia coli, GI48994963, Length=325, Percent_Identity=34.1538461538462, Blast_Score=124, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17568601, Length=362, Percent_Identity=35.3591160220994, Blast_Score=220, Evalue=8e-58,
Organism=Caenorhabditis elegans, GI17565728, Length=346, Percent_Identity=36.1271676300578, Blast_Score=195, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6322002, Length=347, Percent_Identity=39.4812680115274, Blast_Score=220, Evalue=3e-58,
Organism=Saccharomyces cerevisiae, GI6322608, Length=347, Percent_Identity=38.0403458213257, Blast_Score=214, Evalue=2e-56,
Organism=Drosophila melanogaster, GI24641779, Length=338, Percent_Identity=41.4201183431953, Blast_Score=231, Evalue=8e-61,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 40178; Mature: 40047

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLSFDNKVDGSVWLNFDIQPSAHPVSETERTAKLADPGFGRVFTDHMAIVRYDQAKGWY
CCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHCCCCCCHHHHCCEEEEEEECCCCCC
GARVESRANFPLDPATAVLHYAQEIFEGLKAYKRADGGVNLFRPDANARRFRDSADRMAM
CCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHH
AQLPEPVFIEAIEQLVRIDRAWIPGGDGSLYLRPFMIASEVFLGVKPSAEYIFAVIASPV
HHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHH
GSYFKGGPAPVSIWVSENYTRAAIGGTGSVKCGGNYAASLRAQAEAIAHGCDQVVFLDAI
HHHHCCCCCCEEEEEECCCEEEEECCCCCEEECCCHHHHHHHHHHHHHHCCCHHHHHHHH
ERRYIEELGGMNVFFVFDDGSLSTPPLGTILPGITRDSIIALARQAGRTVREEAYTIEQW
HHHHHHHHCCCEEEEEECCCCCCCCCCHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHH
RADAASGKLKEAFACGTAAVISPIGTVRSASGDFTINGGVAGEVAMGLRKQLVDIQYGRA
HHHHCCCCHHHHHHCCHHHHHHCHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCC
EDKHGWIRDVA
CCCCCCCCCCC
>Mature Secondary Structure 
GLSFDNKVDGSVWLNFDIQPSAHPVSETERTAKLADPGFGRVFTDHMAIVRYDQAKGWY
CCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHCCCCCCHHHHCCEEEEEEECCCCCC
GARVESRANFPLDPATAVLHYAQEIFEGLKAYKRADGGVNLFRPDANARRFRDSADRMAM
CCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHH
AQLPEPVFIEAIEQLVRIDRAWIPGGDGSLYLRPFMIASEVFLGVKPSAEYIFAVIASPV
HHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHH
GSYFKGGPAPVSIWVSENYTRAAIGGTGSVKCGGNYAASLRAQAEAIAHGCDQVVFLDAI
HHHHCCCCCCEEEEEECCCEEEEECCCCCEEECCCHHHHHHHHHHHHHHCCCHHHHHHHH
ERRYIEELGGMNVFFVFDDGSLSTPPLGTILPGITRDSIIALARQAGRTVREEAYTIEQW
HHHHHHHHCCCEEEEEECCCCCCCCCCHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHH
RADAASGKLKEAFACGTAAVISPIGTVRSASGDFTINGGVAGEVAMGLRKQLVDIQYGRA
HHHHCCCCHHHHHHCCHHHHHHCHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCC
EDKHGWIRDVA
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]