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The map label for this gene is pth

Identifier: 86751267

GI number: 86751267

Start: 4732673

End: 4733296

Strand: Reverse

Name: pth

Synonym: RPB_4160

Alternate gene names: 86751267

Gene position: 4733296-4732673 (Counterclockwise)

Preceding gene: 86751268

Following gene: 86751266

Centisome position: 88.78

GC content: 63.3

Gene sequence:

>624_bases
ATGCGCTTGTTTGTCGGGCTGGGCAATCCGGGTACGAAGTATCAGGACAACCGGCACAACATCGGGTTCATGGTCATCGA
CGAGATCGCACGGCGTCATGGCTTCTCGCCATGGCGCCGTCGCTTTCAGGGCGAGACATCGGACGGCGCGCTCGACGGCG
AGCGGATCACGCTGTTGAAGCCGCTCACCTACATGAACGAGTCCGGCCGCGCCGTGCAGGAAGCCGCGAGCTTCTACAAG
ATCGGGCAGGGTGAGGTCGCGGTGTTTCACGACGAGATCGAACTGCCGCCGGCGAAGGTCCGGGTCAAGGTCGGCGGCGG
CATCGCCGGACACAACGGCCTGCGCTCGATCTCGGCGCATATCGGCAACGACTATCTGCGGGTGCGGCTCGGCGTCGGTC
ACCCCGGCGTGAAGGAAATGGTTCACGGCCACGTGCTCGGTGATTTCGCCAAGAGCGAGCGGCCCTGGGTCGCGGCGCTA
TGCGAGATCGCCGCCGACAATGCCGGCCTGATCGCCAAGGGCAAGGACGCCAGCTTCGCCAACAAGGTGCATCTGGCGAT
GCAGGCCAAGGGATTTTTGGACGAAGACAAGAAGAAGTCTGGCGACAACAAGGTCGCCAAGTAA

Upstream 100 bases:

>100_bases
CAAGGCTCCGGCGGCTGCGGCTCCCGCCGCCAAGGCCGGCGCGGCCAAGGCTCCGGCGGCTCCGGCGGCCAAGAAGAAGT
AATCCGCGGGACGCCGCGGC

Downstream 100 bases:

>100_bases
CACGCTGTCATGGCCGGGCTCGTCCCGGCCATCCACGTCTGGGCGATGCTGCAAGACGTGGATGCCCGGCACAAGGCCGG
CATGACGAAGCTCCGAGACA

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MRLFVGLGNPGTKYQDNRHNIGFMVIDEIARRHGFSPWRRRFQGETSDGALDGERITLLKPLTYMNESGRAVQEAASFYK
IGQGEVAVFHDEIELPPAKVRVKVGGGIAGHNGLRSISAHIGNDYLRVRLGVGHPGVKEMVHGHVLGDFAKSERPWVAAL
CEIAADNAGLIAKGKDASFANKVHLAMQAKGFLDEDKKKSGDNKVAK

Sequences:

>Translated_207_residues
MRLFVGLGNPGTKYQDNRHNIGFMVIDEIARRHGFSPWRRRFQGETSDGALDGERITLLKPLTYMNESGRAVQEAASFYK
IGQGEVAVFHDEIELPPAKVRVKVGGGIAGHNGLRSISAHIGNDYLRVRLGVGHPGVKEMVHGHVLGDFAKSERPWVAAL
CEIAADNAGLIAKGKDASFANKVHLAMQAKGFLDEDKKKSGDNKVAK
>Mature_207_residues
MRLFVGLGNPGTKYQDNRHNIGFMVIDEIARRHGFSPWRRRFQGETSDGALDGERITLLKPLTYMNESGRAVQEAASFYK
IGQGEVAVFHDEIELPPAKVRVKVGGGIAGHNGLRSISAHIGNDYLRVRLGVGHPGVKEMVHGHVLGDFAKSERPWVAAL
CEIAADNAGLIAKGKDASFANKVHLAMQAKGFLDEDKKKSGDNKVAK

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=168, Percent_Identity=36.3095238095238, Blast_Score=103, Evalue=1e-22,
Organism=Escherichia coli, GI1787455, Length=158, Percent_Identity=46.2025316455696, Blast_Score=115, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH_RHOP2 (Q2ISF8)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_487763.1
- ProteinModelPortal:   Q2ISF8
- SMR:   Q2ISF8
- STRING:   Q2ISF8
- GeneID:   3911968
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_4160
- eggNOG:   COG0193
- HOGENOM:   HBG610927
- OMA:   AMHRLHS
- ProtClustDB:   PRK05426
- BioCyc:   RPAL316058:RPB_4160-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 22569; Mature: 22569

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLFVGLGNPGTKYQDNRHNIGFMVIDEIARRHGFSPWRRRFQGETSDGALDGERITLLK
CEEEEECCCCCCCCCCCCCCCCEEEHHHHHHHCCCCHHHHHHCCCCCCCCCCCCEEEEEC
PLTYMNESGRAVQEAASFYKIGQGEVAVFHDEIELPPAKVRVKVGGGIAGHNGLRSISAH
CHHHCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEEECCCCCCCCHHHHHHHH
IGNDYLRVRLGVGHPGVKEMVHGHVLGDFAKSERPWVAALCEIAADNAGLIAKGKDASFA
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEECCCCCCH
NKVHLAMQAKGFLDEDKKKSGDNKVAK
HHEEEEEECCCCCCCHHHCCCCCCCCC
>Mature Secondary Structure
MRLFVGLGNPGTKYQDNRHNIGFMVIDEIARRHGFSPWRRRFQGETSDGALDGERITLLK
CEEEEECCCCCCCCCCCCCCCCEEEHHHHHHHCCCCHHHHHHCCCCCCCCCCCCEEEEEC
PLTYMNESGRAVQEAASFYKIGQGEVAVFHDEIELPPAKVRVKVGGGIAGHNGLRSISAH
CHHHCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEEECCCCCCCCHHHHHHHH
IGNDYLRVRLGVGHPGVKEMVHGHVLGDFAKSERPWVAALCEIAADNAGLIAKGKDASFA
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEECCCCCCH
NKVHLAMQAKGFLDEDKKKSGDNKVAK
HHEEEEEECCCCCCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA