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The map label for this gene is engD [H]

Identifier: 86751266

GI number: 86751266

Start: 4731466

End: 4732563

Strand: Reverse

Name: engD [H]

Synonym: RPB_4159

Alternate gene names: 86751266

Gene position: 4732563-4731466 (Counterclockwise)

Preceding gene: 86751267

Following gene: 86751264

Centisome position: 88.76

GC content: 64.3

Gene sequence:

>1098_bases
ATGGGATTCAAATGCGGGATCGTCGGGCTGCCGAATGTCGGCAAGTCGACGCTGTTCAATGCGCTGACCGAGACCGCGGC
GGCGCAGGCGGCCAACTATCCGTTCTGCACCATCGAGCCGAATGTCGGCGAGGTCGCGGTCCCGGATCCGCGGCTCGACA
AGCTTGCCGAGGTCGGCAAGTCGCAGCAGATCATTCCGACGCGGCTGACCTTCGTCGACATCGCCGGTCTGGTGAAGGGC
GCTTCCAAGGGTGAAGGCCTCGGCAATCAGTTCCTCGCCACCATCCGCGAGGTCGACGCGATCGCTCATGTGGTGCGCTG
TTTCGAGGACGGTGACATCACCCATGTCGAGGGAAGGGTCGCGCCGATCGCCGACATCGACACGATCGAGACCGAGCTGA
TGCTCGCCGATCTCGACAGCCTCGAGAAGCGCGTCGACAACCTCACCAAGAAGGCCAAGGGCGGCGACAAGGATTCCAAG
GAACAGCTCGAACTGGTCACCCGCGCGCTGACGCTGCTGCGCGAGGGGCGGCCGGCGCGCTTCCTCGAACGCAAGCCGGA
GGAAGAGCGCGCGTTCCGGATGCTCGGGCTGTTGACCTCGAAGCCGGTTCTGTACGTCTGCAACGTCGAGGAAGGCTCCG
CCGCCGAGGGCAATGCATTCTCGCAAGCGGTGATGGCGCGCGCCAAGGACGAAGGCGCGGTCGCGGTGGTGATTTCCGCC
AAGATCGAATCCGAAATCGCGACGCTGTCGAAAGAAGAGCGCGTCGATTTCCTCGATACGCTGGGGCTGCACGAGGCCGG
GCTCGACCGGCTGATCCGCGCCGGCTACGAGCTCTTGCACCTCATCACCTATTTCACCGTCGGCCCCAAGGAAGCCCGCG
CCTGGACCATCACCAAAGGCACCAAGGCGCCGCAGGCGGCGGCCGTGATCCATACCGATTTCGAGAAGGGCTTCATCCGC
GCCGAAACCATCGCCTATGACGACTACACCACGCTCGGCGGCGAAGCCGGCGCCCGCGATGGCGGCAAGCTGCGGCTGGA
AGGCAAGGAATACGTCGTCGCCGACGGCGACGTGATGCATTTCCGATTCAATACGTGA

Upstream 100 bases:

>100_bases
ATGGCCGGGCTCGTCCCGGCCATCCACGTCTGGGCGATGCTGCAAGACGTGGATGCCCGGCACAAGGCCGGCATGACGAA
GCTCCGAGACAGAGGGCGAT

Downstream 100 bases:

>100_bases
GGTTTGGCGAGGGCCGTCACCCTGAGGCGCTCGCCGTCTTCGGCGAGCCTCGAAGGGGGACAGCACAGCGGACCTCGCCC
ATCCTTCGAGGCCCGCGCAT

Product: GTP-dependent nucleic acid-binding protein EngD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MGFKCGIVGLPNVGKSTLFNALTETAAAQAANYPFCTIEPNVGEVAVPDPRLDKLAEVGKSQQIIPTRLTFVDIAGLVKG
ASKGEGLGNQFLATIREVDAIAHVVRCFEDGDITHVEGRVAPIADIDTIETELMLADLDSLEKRVDNLTKKAKGGDKDSK
EQLELVTRALTLLREGRPARFLERKPEEERAFRMLGLLTSKPVLYVCNVEEGSAAEGNAFSQAVMARAKDEGAVAVVISA
KIESEIATLSKEERVDFLDTLGLHEAGLDRLIRAGYELLHLITYFTVGPKEARAWTITKGTKAPQAAAVIHTDFEKGFIR
AETIAYDDYTTLGGEAGARDGGKLRLEGKEYVVADGDVMHFRFNT

Sequences:

>Translated_365_residues
MGFKCGIVGLPNVGKSTLFNALTETAAAQAANYPFCTIEPNVGEVAVPDPRLDKLAEVGKSQQIIPTRLTFVDIAGLVKG
ASKGEGLGNQFLATIREVDAIAHVVRCFEDGDITHVEGRVAPIADIDTIETELMLADLDSLEKRVDNLTKKAKGGDKDSK
EQLELVTRALTLLREGRPARFLERKPEEERAFRMLGLLTSKPVLYVCNVEEGSAAEGNAFSQAVMARAKDEGAVAVVISA
KIESEIATLSKEERVDFLDTLGLHEAGLDRLIRAGYELLHLITYFTVGPKEARAWTITKGTKAPQAAAVIHTDFEKGFIR
AETIAYDDYTTLGGEAGARDGGKLRLEGKEYVVADGDVMHFRFNT
>Mature_364_residues
GFKCGIVGLPNVGKSTLFNALTETAAAQAANYPFCTIEPNVGEVAVPDPRLDKLAEVGKSQQIIPTRLTFVDIAGLVKGA
SKGEGLGNQFLATIREVDAIAHVVRCFEDGDITHVEGRVAPIADIDTIETELMLADLDSLEKRVDNLTKKAKGGDKDSKE
QLELVTRALTLLREGRPARFLERKPEEERAFRMLGLLTSKPVLYVCNVEEGSAAEGNAFSQAVMARAKDEGAVAVVISAK
IESEIATLSKEERVDFLDTLGLHEAGLDRLIRAGYELLHLITYFTVGPKEARAWTITKGTKAPQAAAVIHTDFEKGFIRA
ETIAYDDYTTLGGEAGARDGGKLRLEGKEYVVADGDVMHFRFNT

Specific function: GTP-dependent nucleic acid-binding protein which may act as a translation factor [H]

COG id: COG0012

COG function: function code J; Predicted GTPase, probable translation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI58761500, Length=369, Percent_Identity=45.2574525745258, Blast_Score=308, Evalue=5e-84,
Organism=Homo sapiens, GI58761502, Length=227, Percent_Identity=38.3259911894273, Blast_Score=157, Evalue=1e-38,
Organism=Escherichia coli, GI1787454, Length=364, Percent_Identity=59.3406593406593, Blast_Score=433, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI17509631, Length=367, Percent_Identity=42.5068119891008, Blast_Score=284, Evalue=5e-77,
Organism=Saccharomyces cerevisiae, GI6319499, Length=370, Percent_Identity=40.8108108108108, Blast_Score=266, Evalue=3e-72,
Organism=Saccharomyces cerevisiae, GI6321773, Length=379, Percent_Identity=35.8839050131926, Blast_Score=220, Evalue=3e-58,
Organism=Saccharomyces cerevisiae, GI6321649, Length=289, Percent_Identity=30.7958477508651, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24640873, Length=368, Percent_Identity=44.0217391304348, Blast_Score=297, Evalue=6e-81,
Organism=Drosophila melanogaster, GI24640877, Length=368, Percent_Identity=44.0217391304348, Blast_Score=297, Evalue=6e-81,
Organism=Drosophila melanogaster, GI24640875, Length=368, Percent_Identity=44.0217391304348, Blast_Score=297, Evalue=6e-81,
Organism=Drosophila melanogaster, GI24640879, Length=325, Percent_Identity=41.8461538461538, Blast_Score=243, Evalue=2e-64,
Organism=Drosophila melanogaster, GI24585318, Length=198, Percent_Identity=29.7979797979798, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR004396
- InterPro:   IPR013029
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR012676
- InterPro:   IPR023192 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF06071 YchF-GTPase_C [H]

EC number: NA

Molecular weight: Translated: 39595; Mature: 39463

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFKCGIVGLPNVGKSTLFNALTETAAAQAANYPFCTIEPNVGEVAVPDPRLDKLAEVGK
CCEEECEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEECCCCHHHHHHHCCC
SQQIIPTRLTFVDIAGLVKGASKGEGLGNQFLATIREVDAIAHVVRCFEDGDITHVEGRV
CCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCE
APIADIDTIETELMLADLDSLEKRVDNLTKKAKGGDKDSKEQLELVTRALTLLREGRPAR
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCH
FLERKPEEERAFRMLGLLTSKPVLYVCNVEEGSAAEGNAFSQAVMARAKDEGAVAVVISA
HHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEE
KIESEIATLSKEERVDFLDTLGLHEAGLDRLIRAGYELLHLITYFTVGPKEARAWTITKG
HHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECC
TKAPQAAAVIHTDFEKGFIRAETIAYDDYTTLGGEAGARDGGKLRLEGKEYVVADGDVMH
CCCCCCEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEECCCEEEEECCCEEE
FRFNT
EEECC
>Mature Secondary Structure 
GFKCGIVGLPNVGKSTLFNALTETAAAQAANYPFCTIEPNVGEVAVPDPRLDKLAEVGK
CEEECEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEECCCCHHHHHHHCCC
SQQIIPTRLTFVDIAGLVKGASKGEGLGNQFLATIREVDAIAHVVRCFEDGDITHVEGRV
CCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCE
APIADIDTIETELMLADLDSLEKRVDNLTKKAKGGDKDSKEQLELVTRALTLLREGRPAR
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCH
FLERKPEEERAFRMLGLLTSKPVLYVCNVEEGSAAEGNAFSQAVMARAKDEGAVAVVISA
HHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEE
KIESEIATLSKEERVDFLDTLGLHEAGLDRLIRAGYELLHLITYFTVGPKEARAWTITKG
HHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECC
TKAPQAAAVIHTDFEKGFIRAETIAYDDYTTLGGEAGARDGGKLRLEGKEYVVADGDVMH
CCCCCCEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEECCCEEEEECCCEEE
FRFNT
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA