Click here to switch to the map view.

The map label for this gene is pleD [H]

Identifier: 86751213

GI number: 86751213

Start: 4675441

End: 4676130

Strand: Direct

Name: pleD [H]

Synonym: RPB_4105

Alternate gene names: 86751213

Gene position: 4675441-4676130 (Clockwise)

Preceding gene: 86751212

Following gene: 86751214

Centisome position: 87.69

GC content: 63.19

Gene sequence:

>690_bases
ATGACGATTCGTGTCCTTCACGTCGATGACGAGCCGGACATTCGCGAGATTGCAGCGATCTCGCTCGGTCTGAATTCCGA
GTTCATCACGCGCAGCTGTGGATCTGGCTCCGAAGCCATCGCGGTCGCCGCCGAATGGCCTCCCGACATCATCCTGCTCG
ATGTCATGATGCCGGGAATGGATGGTCCGGCGACGCTCGTGCGCCTGCGCGCGAACGCGATCACCGCCGGCATTCCAGTG
GTGTTCATGACGGCCCGCGCCCAGACCCGCGAGCTCGACCTGTTCCGCTCTCTCGGCGCCGTCGGCGTCATCCCCAAGCC
GTTCGACCCGATGACGCTCGCGGCTTCGGTCCGCTCCCACATCGAACCGATCGGCAGCAAGCTCGCACGGCTACAGTGCG
ATTTTCTGCAGCGTCTCGACAACGATGTCGCCAAGCTGGAACGACTCGCGAACACTCTGAGGTCGGGGAAGGCGAGGAAA
GCGAGCTTGAAGCAAATCGGCGAGATTGCTCACGGGCTCGCCGGCGCCTCCGGCGTATTTGGATTTCCGCAGCTCGGCGA
CCTCGCGGCCGAGCTCGAACAGGCAATCTCCGACGGAGGTGTCGATTCTGTCGACATTGCAAATGTGCGTCGCTCGCTCG
CTCTGCTGCTCGCAGGCATCAAGGCGCGTGACGCCCGAGCGAACTCGTAA

Upstream 100 bases:

>100_bases
GACAAGTGCATGTGATCCTCGTCAAATCACGGACCCCGCTGGATTCCTTGCTGGCGTCGGTGCGGGACTGCCTGGCTCAT
CGCGAACATCGCGAGGTGGC

Downstream 100 bases:

>100_bases
CGTGATGCTCGCGCCGGGCAAATCGAGCGCTCGATCGCGGCGTTCGATCCCGCTGTTGGCGGCGCTGACGATCACGCTGA
CGATCGCAGCCAGCAATCAG

Product: response regulator receiver domain-containing protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MTIRVLHVDDEPDIREIAAISLGLNSEFITRSCGSGSEAIAVAAEWPPDIILLDVMMPGMDGPATLVRLRANAITAGIPV
VFMTARAQTRELDLFRSLGAVGVIPKPFDPMTLAASVRSHIEPIGSKLARLQCDFLQRLDNDVAKLERLANTLRSGKARK
ASLKQIGEIAHGLAGASGVFGFPQLGDLAAELEQAISDGGVDSVDIANVRRSLALLLAGIKARDARANS

Sequences:

>Translated_229_residues
MTIRVLHVDDEPDIREIAAISLGLNSEFITRSCGSGSEAIAVAAEWPPDIILLDVMMPGMDGPATLVRLRANAITAGIPV
VFMTARAQTRELDLFRSLGAVGVIPKPFDPMTLAASVRSHIEPIGSKLARLQCDFLQRLDNDVAKLERLANTLRSGKARK
ASLKQIGEIAHGLAGASGVFGFPQLGDLAAELEQAISDGGVDSVDIANVRRSLALLLAGIKARDARANS
>Mature_228_residues
TIRVLHVDDEPDIREIAAISLGLNSEFITRSCGSGSEAIAVAAEWPPDIILLDVMMPGMDGPATLVRLRANAITAGIPVV
FMTARAQTRELDLFRSLGAVGVIPKPFDPMTLAASVRSHIEPIGSKLARLQCDFLQRLDNDVAKLERLANTLRSGKARKA
SLKQIGEIAHGLAGASGVFGFPQLGDLAAELEQAISDGGVDSVDIANVRRSLALLLAGIKARDARANS

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=106, Percent_Identity=33.9622641509434, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786599, Length=121, Percent_Identity=38.0165289256198, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 24271; Mature: 24140

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS50894 HPT ; PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIRVLHVDDEPDIREIAAISLGLNSEFITRSCGSGSEAIAVAAEWPPDIILLDVMMPGM
CEEEEEEECCCCCHHHHHHHHCCCCCHHHHHCCCCCCCEEEEEECCCCCEEEEEECCCCC
DGPATLVRLRANAITAGIPVVFMTARAQTRELDLFRSLGAVGVIPKPFDPMTLAASVRSH
CCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
IEPIGSKLARLQCDFLQRLDNDVAKLERLANTLRSGKARKASLKQIGEIAHGLAGASGVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCC
GFPQLGDLAAELEQAISDGGVDSVDIANVRRSLALLLAGIKARDARANS
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TIRVLHVDDEPDIREIAAISLGLNSEFITRSCGSGSEAIAVAAEWPPDIILLDVMMPGM
EEEEEEECCCCCHHHHHHHHCCCCCHHHHHCCCCCCCEEEEEECCCCCEEEEEECCCCC
DGPATLVRLRANAITAGIPVVFMTARAQTRELDLFRSLGAVGVIPKPFDPMTLAASVRSH
CCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
IEPIGSKLARLQCDFLQRLDNDVAKLERLANTLRSGKARKASLKQIGEIAHGLAGASGVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCC
GFPQLGDLAAELEQAISDGGVDSVDIANVRRSLALLLAGIKARDARANS
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA