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The map label for this gene is kefB [H]

Identifier: 86750843

GI number: 86750843

Start: 4270476

End: 4272251

Strand: Direct

Name: kefB [H]

Synonym: RPB_3734

Alternate gene names: 86750843

Gene position: 4270476-4272251 (Clockwise)

Preceding gene: 86750841

Following gene: 86750847

Centisome position: 80.1

GC content: 68.47

Gene sequence:

>1776_bases
ATGGCAGCAACGAGCGATATCGGCGTCTACCGGGAGGCCCTGGTTTTTCTCGGGACTGCCGGCGTGCTCATTCCATTGAT
GGCGCGGCTGCGCATCAGCCCGGTGCTCGGCTTTCTGGTCGCCGGATTGGTGCTCGGCCCCTACAGCCTCGGCCGGCTCG
GTGAGGGCCTGCCGTGGCTGCAGGCGATTACCATCACCTCACAGGATGCGGTCGATCGCCTGGCCGAACTCGGCGTCGCC
TTCCTGCTGTTCACCATCGGACTGGAACTGTCATTCGACCGGCTGTGGACGATGCGCCGGCTGGTGTTCGGCCTGGGCAT
GTCGCAGGTGGTGCTGACCACGGCAGCGATCAGCGGTATCGCGCTGGCGTTCGGCAATACCGTCGAAGCCGGTCTGGTGA
TCGGCGCCTGTCTGGCGCTGTCCTCGACCGCCATCGTGCTGCAGCTCCTCGCCGAGCAGAAGCGGCTGGCGACGATCGCC
GGGCGCAGCATCTTCGCGGTGCTGCTGGCGCAGGATCTCGCGGTGGTGCCGATCCTGTTCCTGATCGTGGTGCTGGGCAA
TTCCACCGGCGCTGCGGGCGCAGGCGCGGCCGCGTCTGGATCGATCTGGTTCGGCCTCGGCATGGCGATCGTGCAGGCGG
CCGCCGCGATCCTGCTGATCATCGGCTTCGGACGGCTGGTGCTGCGCCGGCTGTTCCGGCTGGTGGCGCACACCCACAAC
CGCGAATTGTTCATGGCGACCATCCTGTTCGTCGTGGTCGGCACCTCGCTACTGACCCATGTCGCCGGGCTGTCGATGGC
GCTCGGCGCCTTTCTCGCCGGGCTGGTGCTGGCCGAGACCGAATTCCGCCGTCAGGTCGAGGTCGATATCGAGCCGTTCA
AAGGCATGCTGCTCGGGCTGTTCTTCATCTCGGTCGGGATGCGGATCGACGTCGCCGAGGTGGCGCGCTACCCGGCGATG
GTGGCGGCCTCGGTGTTCGGCATGGTGGCGCTGAAGGCGGTGATCATCGCGGCGCTGACGCGCGGCTTCGGGCTGTCATG
GCCGGTGGTGGCGGAAGCGAGCCTGCTGCTCGCCGGCGGCGGCGAATTCGCCTTCCTGGTGCTCGGGCTCGCCTCCTCCT
CGGGGCTGGTCACCCGCGAGGTCGAGCAGTTCATGCTGCTGGTGTCGTCGGGCACCATGCTGCTGACCCCGTTTCTGGCG
CGGCTCTCCCGCAAGGCCAGCAAGCGGGTGCACAACGCGACCGCACTGGCCGACGGCCACGGCGATCCCCGCCCCGCCGA
GGCGGGCGGCATCATCATCGTCGGCTACGGCCGGGTCGGGCGGATCCTCGGCGATATCCTGACGAAGCAGGACAAGGCCT
TCATCGGCATCGAGATCGACCCGGATGCCGTGAGGAAGGCGCGCCGCGCCGGCCACAATCTGGTCTATGGCGACGCCACG
CAGCGCGCCTTCCTGCGCAAATGCGGGCTGGCCGACGCGACGGCGCTGGTGGTGACGATGCACGACGCCAACGCCGCCGA
GCACGTCGTCGCCGCGGCCCGCGCCGAGCGGCCCGATCTCCCGGTGATCGTCCGCGCCCGCGACGCAGCACACGCGATGC
GGCTGTTCAAGCTCGGCGCCACCGACGTCGTCCGCGAAGTGCTGGAAGCCAGTTTCGAAATCGCCAGCACCGTGCTGCAG
ACCGTCGGCATGCCGGTCGGCAAGGTGATCGCCGTCATCCACGACGAACGCGACGCGCGGAAGAAGCAGGTGCGCGAGGG
CATGACGTCGGAGTAG

Upstream 100 bases:

>100_bases
CGCTGGCCTTGGCGACCGAATTGCGATCTTTTGCCGCAGCGAGACTCAATTTGATCGAACCCCTCGCCGGCACGGCGATG
CCGTGATTGATGGAATCATC

Downstream 100 bases:

>100_bases
TCGCGGCCGACGTGGCGCGAGCCCGAGCGTCCTGCGTTCGGGCTCGGGTGCGTTCGGACTCGGGCAAGGCTGCTACTTCA
GCACCACGATCCCCGGCTCG

Product: sodium/hydrogen exchanger

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 591; Mature: 590

Protein sequence:

>591_residues
MAATSDIGVYREALVFLGTAGVLIPLMARLRISPVLGFLVAGLVLGPYSLGRLGEGLPWLQAITITSQDAVDRLAELGVA
FLLFTIGLELSFDRLWTMRRLVFGLGMSQVVLTTAAISGIALAFGNTVEAGLVIGACLALSSTAIVLQLLAEQKRLATIA
GRSIFAVLLAQDLAVVPILFLIVVLGNSTGAAGAGAAASGSIWFGLGMAIVQAAAAILLIIGFGRLVLRRLFRLVAHTHN
RELFMATILFVVVGTSLLTHVAGLSMALGAFLAGLVLAETEFRRQVEVDIEPFKGMLLGLFFISVGMRIDVAEVARYPAM
VAASVFGMVALKAVIIAALTRGFGLSWPVVAEASLLLAGGGEFAFLVLGLASSSGLVTREVEQFMLLVSSGTMLLTPFLA
RLSRKASKRVHNATALADGHGDPRPAEAGGIIIVGYGRVGRILGDILTKQDKAFIGIEIDPDAVRKARRAGHNLVYGDAT
QRAFLRKCGLADATALVVTMHDANAAEHVVAAARAERPDLPVIVRARDAAHAMRLFKLGATDVVREVLEASFEIASTVLQ
TVGMPVGKVIAVIHDERDARKKQVREGMTSE

Sequences:

>Translated_591_residues
MAATSDIGVYREALVFLGTAGVLIPLMARLRISPVLGFLVAGLVLGPYSLGRLGEGLPWLQAITITSQDAVDRLAELGVA
FLLFTIGLELSFDRLWTMRRLVFGLGMSQVVLTTAAISGIALAFGNTVEAGLVIGACLALSSTAIVLQLLAEQKRLATIA
GRSIFAVLLAQDLAVVPILFLIVVLGNSTGAAGAGAAASGSIWFGLGMAIVQAAAAILLIIGFGRLVLRRLFRLVAHTHN
RELFMATILFVVVGTSLLTHVAGLSMALGAFLAGLVLAETEFRRQVEVDIEPFKGMLLGLFFISVGMRIDVAEVARYPAM
VAASVFGMVALKAVIIAALTRGFGLSWPVVAEASLLLAGGGEFAFLVLGLASSSGLVTREVEQFMLLVSSGTMLLTPFLA
RLSRKASKRVHNATALADGHGDPRPAEAGGIIIVGYGRVGRILGDILTKQDKAFIGIEIDPDAVRKARRAGHNLVYGDAT
QRAFLRKCGLADATALVVTMHDANAAEHVVAAARAERPDLPVIVRARDAAHAMRLFKLGATDVVREVLEASFEIASTVLQ
TVGMPVGKVIAVIHDERDARKKQVREGMTSE
>Mature_590_residues
AATSDIGVYREALVFLGTAGVLIPLMARLRISPVLGFLVAGLVLGPYSLGRLGEGLPWLQAITITSQDAVDRLAELGVAF
LLFTIGLELSFDRLWTMRRLVFGLGMSQVVLTTAAISGIALAFGNTVEAGLVIGACLALSSTAIVLQLLAEQKRLATIAG
RSIFAVLLAQDLAVVPILFLIVVLGNSTGAAGAGAAASGSIWFGLGMAIVQAAAAILLIIGFGRLVLRRLFRLVAHTHNR
ELFMATILFVVVGTSLLTHVAGLSMALGAFLAGLVLAETEFRRQVEVDIEPFKGMLLGLFFISVGMRIDVAEVARYPAMV
AASVFGMVALKAVIIAALTRGFGLSWPVVAEASLLLAGGGEFAFLVLGLASSSGLVTREVEQFMLLVSSGTMLLTPFLAR
LSRKASKRVHNATALADGHGDPRPAEAGGIIIVGYGRVGRILGDILTKQDKAFIGIEIDPDAVRKARRAGHNLVYGDATQ
RAFLRKCGLADATALVVTMHDANAAEHVVAAARAERPDLPVIVRARDAAHAMRLFKLGATDVVREVLEASFEIASTVLQT
VGMPVGKVIAVIHDERDARKKQVREGMTSE

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=404, Percent_Identity=25.990099009901, Blast_Score=73, Evalue=9e-13,
Organism=Escherichia coli, GI1786232, Length=557, Percent_Identity=34.8294434470377, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI1789749, Length=568, Percent_Identity=36.2676056338028, Blast_Score=292, Evalue=4e-80,
Organism=Escherichia coli, GI1786685, Length=557, Percent_Identity=28.3662477558348, Blast_Score=164, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 62325; Mature: 62193

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAATSDIGVYREALVFLGTAGVLIPLMARLRISPVLGFLVAGLVLGPYSLGRLGEGLPWL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHH
QAITITSQDAVDRLAELGVAFLLFTIGLELSFDRLWTMRRLVFGLGMSQVVLTTAAISGI
HEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
ALAFGNTVEAGLVIGACLALSSTAIVLQLLAEQKRLATIAGRSIFAVLLAQDLAVVPILF
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIVVLGNSTGAAGAGAAASGSIWFGLGMAIVQAAAAILLIIGFGRLVLRRLFRLVAHTHN
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RELFMATILFVVVGTSLLTHVAGLSMALGAFLAGLVLAETEFRRQVEVDIEPFKGMLLGL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHH
FFISVGMRIDVAEVARYPAMVAASVFGMVALKAVIIAALTRGFGLSWPVVAEASLLLAGG
HHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEEECC
GEFAFLVLGLASSSGLVTREVEQFMLLVSSGTMLLTPFLARLSRKASKRVHNATALADGH
CCEEEHEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEECCC
GDPRPAEAGGIIIVGYGRVGRILGDILTKQDKAFIGIEIDPDAVRKARRAGHNLVYGDAT
CCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHCCCCEEECCHH
QRAFLRKCGLADATALVVTMHDANAAEHVVAAARAERPDLPVIVRARDAAHAMRLFKLGA
HHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHCCH
TDVVREVLEASFEIASTVLQTVGMPVGKVIAVIHDERDARKKQVREGMTSE
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AATSDIGVYREALVFLGTAGVLIPLMARLRISPVLGFLVAGLVLGPYSLGRLGEGLPWL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHH
QAITITSQDAVDRLAELGVAFLLFTIGLELSFDRLWTMRRLVFGLGMSQVVLTTAAISGI
HEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
ALAFGNTVEAGLVIGACLALSSTAIVLQLLAEQKRLATIAGRSIFAVLLAQDLAVVPILF
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIVVLGNSTGAAGAGAAASGSIWFGLGMAIVQAAAAILLIIGFGRLVLRRLFRLVAHTHN
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RELFMATILFVVVGTSLLTHVAGLSMALGAFLAGLVLAETEFRRQVEVDIEPFKGMLLGL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHH
FFISVGMRIDVAEVARYPAMVAASVFGMVALKAVIIAALTRGFGLSWPVVAEASLLLAGG
HHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEEECC
GEFAFLVLGLASSSGLVTREVEQFMLLVSSGTMLLTPFLARLSRKASKRVHNATALADGH
CCEEEHEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEECCC
GDPRPAEAGGIIIVGYGRVGRILGDILTKQDKAFIGIEIDPDAVRKARRAGHNLVYGDAT
CCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHCCCCEEECCHH
QRAFLRKCGLADATALVVTMHDANAAEHVVAAARAERPDLPVIVRARDAAHAMRLFKLGA
HHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHCCH
TDVVREVLEASFEIASTVLQTVGMPVGKVIAVIHDERDARKKQVREGMTSE
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA