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The map label for this gene is purB [H]

Identifier: 86750803

GI number: 86750803

Start: 4232587

End: 4233963

Strand: Direct

Name: purB [H]

Synonym: RPB_3693

Alternate gene names: 86750803

Gene position: 4232587-4233963 (Clockwise)

Preceding gene: 86750801

Following gene: 86750804

Centisome position: 79.39

GC content: 65.07

Gene sequence:

>1377_bases
GTGGACTGTGGCCTCCCCGCCGCGCTTCTGCTAAAGCGCGGCGTAATCCCCCAACACGAAGAGTCTGTGATGATCCCGCG
CTACACCCGTCCGGAAATGGCTGCCATCTGGGAGCCGCTCACCCGCTTCAAGATCTGGTTCGAGATCGAGGCCCACGCCT
CGGACGCGCAGGCGGCGCTCGGCGTGATCCCGAAAGAGGCGGCGGAGACGATCTGGGCCAAGGGCAAGGACGCGGCGTTC
GACGTGGCGCGGATCGACGAGATCGAGCGCGAGGTCAAGCACGACGTCATCGCGTTTCTGACGCATCTGGCCGAGTTCGT
GGGTCCCTTGTCGCGCTTCGTGCATCAGGGCATGACCTCGTCGGACGTGCTCGACACCTGCTTCAACGTGCAGCTGGTGC
GCGCCTCCGACATCCTGCTGGCCGATCTCGACAAGGTTCTCGCGGCGCTGAAGACGCGCGCGTTCGAACACAAGATGACG
CCGTCGATCGGCCGCTCGCACGGCATCCATGCCGAGCCGGTGACGTTCGGCCTGAAGATGGCCTACGCCTACGCCGAATT
CTCCCGCGCCCGCGATCGCCTGGTCGCCGCGCGCAAAGAAGTCGCGACCTGCGCGATCTCCGGTTCGGTCGGCACCTTCG
CGCATATCGATCCGCGGGTCGAAGAACACGTCGCCAAGGCAATGGGCCTCGCGGTCGAGCCGGTGTCGACGCAAGTGATC
CCGCGCGATCGCCACGCGATGTACTTCGCCACGCTGGGCGTGATCGCCTCGTCGGTCGAGCGGCTCGCCACCGAGCTGCG
GCATCTGCAGCGCACCGAGGTGCTCGAGGCCGAGGAGTACTTCTCCGAGGGCCAGAAGGGCTCGTCCTCGATGCCGCACA
AGCGCAACCCGGTGTTGACCGAGAATTTGACCGGCCTCGCCCGGATGGTGCGCGCCTATGCGATGCCGGCGATGGAGAAC
GTCGTGCTGTGGCACGAGCGCGACATCTCGCATTCCTCCGCCGAGCGCATGATCGCGCCGGACTCCACCGTGACGCTCGA
CTTCGCGCTCAATCGACTGGCGGGCGTGATCGAGAAGCTGGTGGTGTATCCCGACAACATGGCGAAAAATCTCGACCGCC
TCGGCGGCCTGATCCATTCGCAGCGCCTGCTCACCGCGCTGACCCAGAAGGGCTGTTCGCGCGAGGAAAGCTACAAGCTG
GTGCAGCGCAACGCGATGCCGGTGTGGCGCGGCGAAGGCGACTTCCTGACGCTGCTGAAGAAGGATCCGGACATGAAGAA
GTACATGTCGGATGCGGAGATCGAGGAGCAGTTCGACCTCGGCTATCACCTCAAGCACGTCGACACGATCTTCCGGCGGG
TGTTCGGCGAGAACTGA

Upstream 100 bases:

>100_bases
ACAAATTCGCTCGACCCCAGCACAGATTCTGTTCCTTCCATCCAACCATCTATTAATGGATATTATCCTAGGATTATTCA
CTTATGTCAGGGAAGATCTG

Downstream 100 bases:

>100_bases
GCGACGCCTCTTCACCCTCCCCTGGAGGGGGAGGGTCGGCACGCAGCCCGCAGTGCGGGATGCGTGACGGGGTGGGGTGA
GAAGCGGGCACGGCGGACGA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MDCGLPAALLLKRGVIPQHEESVMIPRYTRPEMAAIWEPLTRFKIWFEIEAHASDAQAALGVIPKEAAETIWAKGKDAAF
DVARIDEIEREVKHDVIAFLTHLAEFVGPLSRFVHQGMTSSDVLDTCFNVQLVRASDILLADLDKVLAALKTRAFEHKMT
PSIGRSHGIHAEPVTFGLKMAYAYAEFSRARDRLVAARKEVATCAISGSVGTFAHIDPRVEEHVAKAMGLAVEPVSTQVI
PRDRHAMYFATLGVIASSVERLATELRHLQRTEVLEAEEYFSEGQKGSSSMPHKRNPVLTENLTGLARMVRAYAMPAMEN
VVLWHERDISHSSAERMIAPDSTVTLDFALNRLAGVIEKLVVYPDNMAKNLDRLGGLIHSQRLLTALTQKGCSREESYKL
VQRNAMPVWRGEGDFLTLLKKDPDMKKYMSDAEIEEQFDLGYHLKHVDTIFRRVFGEN

Sequences:

>Translated_458_residues
MDCGLPAALLLKRGVIPQHEESVMIPRYTRPEMAAIWEPLTRFKIWFEIEAHASDAQAALGVIPKEAAETIWAKGKDAAF
DVARIDEIEREVKHDVIAFLTHLAEFVGPLSRFVHQGMTSSDVLDTCFNVQLVRASDILLADLDKVLAALKTRAFEHKMT
PSIGRSHGIHAEPVTFGLKMAYAYAEFSRARDRLVAARKEVATCAISGSVGTFAHIDPRVEEHVAKAMGLAVEPVSTQVI
PRDRHAMYFATLGVIASSVERLATELRHLQRTEVLEAEEYFSEGQKGSSSMPHKRNPVLTENLTGLARMVRAYAMPAMEN
VVLWHERDISHSSAERMIAPDSTVTLDFALNRLAGVIEKLVVYPDNMAKNLDRLGGLIHSQRLLTALTQKGCSREESYKL
VQRNAMPVWRGEGDFLTLLKKDPDMKKYMSDAEIEEQFDLGYHLKHVDTIFRRVFGEN
>Mature_458_residues
MDCGLPAALLLKRGVIPQHEESVMIPRYTRPEMAAIWEPLTRFKIWFEIEAHASDAQAALGVIPKEAAETIWAKGKDAAF
DVARIDEIEREVKHDVIAFLTHLAEFVGPLSRFVHQGMTSSDVLDTCFNVQLVRASDILLADLDKVLAALKTRAFEHKMT
PSIGRSHGIHAEPVTFGLKMAYAYAEFSRARDRLVAARKEVATCAISGSVGTFAHIDPRVEEHVAKAMGLAVEPVSTQVI
PRDRHAMYFATLGVIASSVERLATELRHLQRTEVLEAEEYFSEGQKGSSSMPHKRNPVLTENLTGLARMVRAYAMPAMEN
VVLWHERDISHSSAERMIAPDSTVTLDFALNRLAGVIEKLVVYPDNMAKNLDRLGGLIHSQRLLTALTQKGCSREESYKL
VQRNAMPVWRGEGDFLTLLKKDPDMKKYMSDAEIEEQFDLGYHLKHVDTIFRRVFGEN

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=413, Percent_Identity=26.634382566586, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI183227688, Length=406, Percent_Identity=26.8472906403941, Blast_Score=131, Evalue=1e-30,
Organism=Escherichia coli, GI1787376, Length=370, Percent_Identity=28.3783783783784, Blast_Score=89, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17508577, Length=404, Percent_Identity=25.2475247524752, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI32564234, Length=368, Percent_Identity=25.5434782608696, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323391, Length=420, Percent_Identity=27.3809523809524, Blast_Score=133, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24647570, Length=442, Percent_Identity=24.4343891402715, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 51315; Mature: 51315

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDCGLPAALLLKRGVIPQHEESVMIPRYTRPEMAAIWEPLTRFKIWFEIEAHASDAQAAL
CCCCCCHHHHHHCCCCCCCCCCEECCCCCCCHHHHHHHHHHHEEEEEEEECCCCCHHHHH
GVIPKEAAETIWAKGKDAAFDVARIDEIEREVKHDVIAFLTHLAEFVGPLSRFVHQGMTS
HCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SDVLDTCFNVQLVRASDILLADLDKVLAALKTRAFEHKMTPSIGRSHGIHAEPVTFGLKM
HHHHHHHHCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
AYAYAEFSRARDRLVAARKEVATCAISGSVGTFAHIDPRVEEHVAKAMGLAVEPVSTQVI
HHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEECCHHHHHHHHHHHCCEECCCCCCCC
PRDRHAMYFATLGVIASSVERLATELRHLQRTEVLEAEEYFSEGQKGSSSMPHKRNPVLT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCH
ENLTGLARMVRAYAMPAMENVVLWHERDISHSSAERMIAPDSTVTLDFALNRLAGVIEKL
HHHHHHHHHHHHHHCHHHHHEEEEECCCCCCHHHCCEECCCCCEEHHHHHHHHHHHHHHH
VVYPDNMAKNLDRLGGLIHSQRLLTALTQKGCSREESYKLVQRNAMPVWRGEGDFLTLLK
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEECCCCEEEEEC
KDPDMKKYMSDAEIEEQFDLGYHLKHVDTIFRRVFGEN
CCCCHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDCGLPAALLLKRGVIPQHEESVMIPRYTRPEMAAIWEPLTRFKIWFEIEAHASDAQAAL
CCCCCCHHHHHHCCCCCCCCCCEECCCCCCCHHHHHHHHHHHEEEEEEEECCCCCHHHHH
GVIPKEAAETIWAKGKDAAFDVARIDEIEREVKHDVIAFLTHLAEFVGPLSRFVHQGMTS
HCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SDVLDTCFNVQLVRASDILLADLDKVLAALKTRAFEHKMTPSIGRSHGIHAEPVTFGLKM
HHHHHHHHCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
AYAYAEFSRARDRLVAARKEVATCAISGSVGTFAHIDPRVEEHVAKAMGLAVEPVSTQVI
HHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEECCHHHHHHHHHHHCCEECCCCCCCC
PRDRHAMYFATLGVIASSVERLATELRHLQRTEVLEAEEYFSEGQKGSSSMPHKRNPVLT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCH
ENLTGLARMVRAYAMPAMENVVLWHERDISHSSAERMIAPDSTVTLDFALNRLAGVIEKL
HHHHHHHHHHHHHHCHHHHHEEEEECCCCCCHHHCCEECCCCCEEHHHHHHHHHHHHHHH
VVYPDNMAKNLDRLGGLIHSQRLLTALTQKGCSREESYKLVQRNAMPVWRGEGDFLTLLK
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEECCCCEEEEEC
KDPDMKKYMSDAEIEEQFDLGYHLKHVDTIFRRVFGEN
CCCCHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]