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The map label for this gene is braE [H]

Identifier: 86750792

GI number: 86750792

Start: 4221545

End: 4222876

Strand: Direct

Name: braE [H]

Synonym: RPB_3682

Alternate gene names: 86750792

Gene position: 4221545-4222876 (Clockwise)

Preceding gene: 86750791

Following gene: 86750793

Centisome position: 79.18

GC content: 66.59

Gene sequence:

>1332_bases
GTGGCTCATCCCGTTCACGCCGCCGCTGCCACCGCCGCCAAACCGGCCGCGAATATCGGTTTCATCCTCAAGAAAGCCCT
GATCAGCGCCCTCGTGGCGCTGGTTCTGTTTTCGCTGATGATCGGCGTGCGAACCGAGGCCGGGCCCGAAGGACAACTGA
TCTACTGGACCCGGTTCGGGGACCTTGCCGGCATGGTCGCCGCGGTATTCGTCGGCTCGATCGTCGTCGAACTGCTGCGC
CATTGGTGGGGACCGGTCGAGACGCCGCGGCTGATGCCCGAAGGCGCCCGCAAGACGCTCGGTGTCGCCGGCCGCGCGGT
CGCGCCGGTGCTGCTGATCTTCACGATTCTGGTGCCGGTGATCTTCTACGACCAGCGCTACATTCTCGACCTCGGCATTC
TCGTCCTGACCTATGTGATGCTCGGCTGGGGCCTGAATATCGTGGTCGGCCTCGCCGGCCTGCTGGACCTCGGCTACGTC
GCGTTCTACGCCGTCGGCGCCTATTCCTACGCGCTGCTCGCCACCAATTTCGACCTGTCGTTCTGGATCTGCCTGCCGCT
CGCCGGCATCCTCGCCGCGTTCTGGGGCGTGCTGCTCGGCTTTCCGGTGCTGCGGCTGCGCGGCGACTATCTGGCCATCG
TCACGCTGGCCTTCGGCGAAATCATCCGCCTCGTCATCATCAATTGGCAGAGCCTGACCGGCGGTCCGAACGGCATCAGC
GGCATTCCGCGGCCGACGCTGTTCGGCATCCCGCTGGTGCCTGGCGAGAACGGCCTCGCCGCGATGCTCGGGATTCCATT
CTCGCCGTCGCACCGGCTGGTGTTCCTGTTCTATCTGATCCTGGCGCTGGCGCTGATCACCAACTGGGCGACGATCCGGC
TTCGCCGGCTGCCGATCGGCCGCGCCTGGGAAGCGCTGCGCGAGGACGAGGTCGCCTGCCGCGCGCTCGGCATCAACACC
ACCACGACCAAGTTGACGGCGTTCGCCACCGGCGCGATGTTCGGCGGCTTCGCCGGCGCGTTCTTCGCCACAAGGCAGGG
CTTCATCTCGCCGGAATCCTTCACCTTCCAGGAATCCGCGCTGGTGCTGGCGATCGTCGTGCTCGGCGGCATGGGCTCGC
AGCTCGGCGTCGCGCTGGCCGCGCTGGCGCTGATCGGCGGCTTCGAGCTGTTCCGCGGGCTCGATCAGTTCCGCATGCTG
GTGTTCGGCCTGTCGATGGTGCTGCTGATGGTATGGCGACCGCGCGGCCTGATCGGCCACCGCGCGCCGACCGTGTATCT
GCAGCACAATCAGGCGATCTCGTCCGACCTCGTCAAGGAGGGCCATGGATGA

Upstream 100 bases:

>100_bases
CAAGGACGTCGCGGCGTTCTCGATCCTCATCGTCGTGTTGATCTTCATGCCGACCGGCCTGCTGGGCCGGCCGGAAGTCG
AAAAAGTCTGAGCGGGCGCA

Downstream 100 bases:

>100_bases
GCAACGCCCCCCCCATTCTCCGCGTCGAGGGACTGACAATGCGGTTCGGCGGCATCGTCGCCGTCAACGAATTGTCGTTC
GAGGCGCAGCGCGGCAAGAT

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MAHPVHAAAATAAKPAANIGFILKKALISALVALVLFSLMIGVRTEAGPEGQLIYWTRFGDLAGMVAAVFVGSIVVELLR
HWWGPVETPRLMPEGARKTLGVAGRAVAPVLLIFTILVPVIFYDQRYILDLGILVLTYVMLGWGLNIVVGLAGLLDLGYV
AFYAVGAYSYALLATNFDLSFWICLPLAGILAAFWGVLLGFPVLRLRGDYLAIVTLAFGEIIRLVIINWQSLTGGPNGIS
GIPRPTLFGIPLVPGENGLAAMLGIPFSPSHRLVFLFYLILALALITNWATIRLRRLPIGRAWEALREDEVACRALGINT
TTTKLTAFATGAMFGGFAGAFFATRQGFISPESFTFQESALVLAIVVLGGMGSQLGVALAALALIGGFELFRGLDQFRML
VFGLSMVLLMVWRPRGLIGHRAPTVYLQHNQAISSDLVKEGHG

Sequences:

>Translated_443_residues
MAHPVHAAAATAAKPAANIGFILKKALISALVALVLFSLMIGVRTEAGPEGQLIYWTRFGDLAGMVAAVFVGSIVVELLR
HWWGPVETPRLMPEGARKTLGVAGRAVAPVLLIFTILVPVIFYDQRYILDLGILVLTYVMLGWGLNIVVGLAGLLDLGYV
AFYAVGAYSYALLATNFDLSFWICLPLAGILAAFWGVLLGFPVLRLRGDYLAIVTLAFGEIIRLVIINWQSLTGGPNGIS
GIPRPTLFGIPLVPGENGLAAMLGIPFSPSHRLVFLFYLILALALITNWATIRLRRLPIGRAWEALREDEVACRALGINT
TTTKLTAFATGAMFGGFAGAFFATRQGFISPESFTFQESALVLAIVVLGGMGSQLGVALAALALIGGFELFRGLDQFRML
VFGLSMVLLMVWRPRGLIGHRAPTVYLQHNQAISSDLVKEGHG
>Mature_442_residues
AHPVHAAAATAAKPAANIGFILKKALISALVALVLFSLMIGVRTEAGPEGQLIYWTRFGDLAGMVAAVFVGSIVVELLRH
WWGPVETPRLMPEGARKTLGVAGRAVAPVLLIFTILVPVIFYDQRYILDLGILVLTYVMLGWGLNIVVGLAGLLDLGYVA
FYAVGAYSYALLATNFDLSFWICLPLAGILAAFWGVLLGFPVLRLRGDYLAIVTLAFGEIIRLVIINWQSLTGGPNGISG
IPRPTLFGIPLVPGENGLAAMLGIPFSPSHRLVFLFYLILALALITNWATIRLRRLPIGRAWEALREDEVACRALGINTT
TTKLTAFATGAMFGGFAGAFFATRQGFISPESFTFQESALVLAIVVLGGMGSQLGVALAALALIGGFELFRGLDQFRMLV
FGLSMVLLMVWRPRGLIGHRAPTVYLQHNQAISSDLVKEGHG

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=316, Percent_Identity=54.4303797468354, Blast_Score=326, Evalue=2e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 47618; Mature: 47487

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHPVHAAAATAAKPAANIGFILKKALISALVALVLFSLMIGVRTEAGPEGQLIYWTRFG
CCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECH
DLAGMVAAVFVGSIVVELLRHWWGPVETPRLMPEGARKTLGVAGRAVAPVLLIFTILVPV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHH
IFYDQRYILDLGILVLTYVMLGWGLNIVVGLAGLLDLGYVAFYAVGAYSYALLATNFDLS
HHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCH
FWICLPLAGILAAFWGVLLGFPVLRLRGDYLAIVTLAFGEIIRLVIINWQSLTGGPNGIS
HHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHEECHHCCCCCCCCC
GIPRPTLFGIPLVPGENGLAAMLGIPFSPSHRLVFLFYLILALALITNWATIRLRRLPIG
CCCCCCEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHEEEEECCCH
RAWEALREDEVACRALGINTTTTKLTAFATGAMFGGFAGAFFATRQGFISPESFTFQESA
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
LVLAIVVLGGMGSQLGVALAALALIGGFELFRGLDQFRMLVFGLSMVLLMVWRPRGLIGH
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
RAPTVYLQHNQAISSDLVKEGHG
CCCEEEEECCCHHHHHHHHCCCC
>Mature Secondary Structure 
AHPVHAAAATAAKPAANIGFILKKALISALVALVLFSLMIGVRTEAGPEGQLIYWTRFG
CCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECH
DLAGMVAAVFVGSIVVELLRHWWGPVETPRLMPEGARKTLGVAGRAVAPVLLIFTILVPV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHH
IFYDQRYILDLGILVLTYVMLGWGLNIVVGLAGLLDLGYVAFYAVGAYSYALLATNFDLS
HHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCH
FWICLPLAGILAAFWGVLLGFPVLRLRGDYLAIVTLAFGEIIRLVIINWQSLTGGPNGIS
HHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHEECHHCCCCCCCCC
GIPRPTLFGIPLVPGENGLAAMLGIPFSPSHRLVFLFYLILALALITNWATIRLRRLPIG
CCCCCCEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHEEEEECCCH
RAWEALREDEVACRALGINTTTTKLTAFATGAMFGGFAGAFFATRQGFISPESFTFQESA
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
LVLAIVVLGGMGSQLGVALAALALIGGFELFRGLDQFRMLVFGLSMVLLMVWRPRGLIGH
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
RAPTVYLQHNQAISSDLVKEGHG
CCCEEEEECCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]