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The map label for this gene is thcD [H]

Identifier: 86750766

GI number: 86750766

Start: 4196618

End: 4197835

Strand: Reverse

Name: thcD [H]

Synonym: RPB_3656

Alternate gene names: 86750766

Gene position: 4197835-4196618 (Counterclockwise)

Preceding gene: 86750767

Following gene: 86750763

Centisome position: 78.73

GC content: 65.52

Gene sequence:

>1218_bases
ATGAACGACACGGTCTTGATTGCTGGAGCCGGACACGCCGGATTTCAGGTCGCCGTCTCGCTGCGCCAAGCCAAGTACAC
CGGCCGCATCGCCCTGATCAACGACGAGAAGCATCTGCCGTATCAGCGGCCGCCGCTGTCGAAGGCCTATCTCAAGAGCG
GTGGCGACCCCAATTCGTTGATGTTCCGGCCGGAAAAATTCTTCCAGGATCAGACCATCGAATTGATCGACGGCCGCGCC
GTCGCGATCGACCGCGACGCCAAGACGCTGCTGCTCGCCTCCGGCGACAAGATCGAATACGGCCATCTGGTGCTCGCCAC
CGGCGCCCGCAACCGCCAGCTCGACGTGCCGAACGCCACGCTGGACGACGTGCTGTATCTGCGCACGCTCGACGAAAGCG
AAGTGGTGCGGCAGCGGATGCCGGAGAAGAAGCACGTCGTGGTGATCGGCGCCGGATTCATCGGACTGGAATTCGCCGCC
ACCGCACGCGGCAAGGGCATGGAAGTCGACGTCGTCGAACTCGCCCCGCGGGTGATGGCGCGCGCGGTGACGCCGGAGAT
CTCGAGCTACTTCCACGACCGCCACACCGCGGCCGGCATTCGTATCCACTACGGGGTCCGCGCCACTGAGATCGAAGGCG
AAGACGGTCGCGTCACCGGCGTCGCGCTGAGCGACGGCCGGACCTTGCCGTGCGATCTGGTGGTCGTCGGGGTCGGCGTG
ATCCCGAATGTCGAACTGGCCTCGGCGGCCGGGTTGCCGACCGCAGCCGGCATCATCGTCAACGAGCAGCTTCTCACCGA
AGATCCGAACATCTCGGCCATCGGCGATTGCGCGCTGTTCAACAGCGTACGGTTCGGCGAGGTGATGCGCGTGGAATCGG
TGCAGAACGCCACCGACCAGGCGCGCTGCGTCGCGGCGCGGCTGACGGGTTCCCCCGCGACTTATGACGGCTATCCGTGG
TTCTGGTCCGATCAGGGCGACGACAAGCTGCAGATCGCCGGTCTCACCGCCGGCTTCGACCAGGTCGTGCTGCGCGGCAG
CGTCGCCGAGCGCTCGTTCTCGGCGTTCTGCTACAAGGACGGCCAACTGATCGGCGTCGAATCCGTCAACCGCGCGGCCG
ACCATGTGTTCGGCCGCAAAATCCTGCCGCTCGGCAAGTCGGTGACGCCGGAGCAGGCCGCCGATCTCAGCTTCGACCTA
AAGAAGGCGATGGCGTAA

Upstream 100 bases:

>100_bases
TCCGGCAAGCTGACCGGCCTGACGTCTTTGTGCTAGAGGCTGCCGCACCCTTCGCCCGCCTGGCGCGTGCCGCGCCTTAT
CCAACGCCACGAGTGACATG

Downstream 100 bases:

>100_bases
CGGCGTCGGTCCGCCGCAACACTGTCATTGCGAGCGGAGTGAAGAAATCGAGCTTCTGAAGAGCGCTAACATCGCTTTGG
ATCAGAAGCGGGAGTTGCTA

Product: FAD-dependent pyridine nucleotide-disulphide oxidoreductase

Products: oxidized ferredoxin; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MNDTVLIAGAGHAGFQVAVSLRQAKYTGRIALINDEKHLPYQRPPLSKAYLKSGGDPNSLMFRPEKFFQDQTIELIDGRA
VAIDRDAKTLLLASGDKIEYGHLVLATGARNRQLDVPNATLDDVLYLRTLDESEVVRQRMPEKKHVVVIGAGFIGLEFAA
TARGKGMEVDVVELAPRVMARAVTPEISSYFHDRHTAAGIRIHYGVRATEIEGEDGRVTGVALSDGRTLPCDLVVVGVGV
IPNVELASAAGLPTAAGIIVNEQLLTEDPNISAIGDCALFNSVRFGEVMRVESVQNATDQARCVAARLTGSPATYDGYPW
FWSDQGDDKLQIAGLTAGFDQVVLRGSVAERSFSAFCYKDGQLIGVESVNRAADHVFGRKILPLGKSVTPEQAADLSFDL
KKAMA

Sequences:

>Translated_405_residues
MNDTVLIAGAGHAGFQVAVSLRQAKYTGRIALINDEKHLPYQRPPLSKAYLKSGGDPNSLMFRPEKFFQDQTIELIDGRA
VAIDRDAKTLLLASGDKIEYGHLVLATGARNRQLDVPNATLDDVLYLRTLDESEVVRQRMPEKKHVVVIGAGFIGLEFAA
TARGKGMEVDVVELAPRVMARAVTPEISSYFHDRHTAAGIRIHYGVRATEIEGEDGRVTGVALSDGRTLPCDLVVVGVGV
IPNVELASAAGLPTAAGIIVNEQLLTEDPNISAIGDCALFNSVRFGEVMRVESVQNATDQARCVAARLTGSPATYDGYPW
FWSDQGDDKLQIAGLTAGFDQVVLRGSVAERSFSAFCYKDGQLIGVESVNRAADHVFGRKILPLGKSVTPEQAADLSFDL
KKAMA
>Mature_405_residues
MNDTVLIAGAGHAGFQVAVSLRQAKYTGRIALINDEKHLPYQRPPLSKAYLKSGGDPNSLMFRPEKFFQDQTIELIDGRA
VAIDRDAKTLLLASGDKIEYGHLVLATGARNRQLDVPNATLDDVLYLRTLDESEVVRQRMPEKKHVVVIGAGFIGLEFAA
TARGKGMEVDVVELAPRVMARAVTPEISSYFHDRHTAAGIRIHYGVRATEIEGEDGRVTGVALSDGRTLPCDLVVVGVGV
IPNVELASAAGLPTAAGIIVNEQLLTEDPNISAIGDCALFNSVRFGEVMRVESVQNATDQARCVAARLTGSPATYDGYPW
FWSDQGDDKLQIAGLTAGFDQVVLRGSVAERSFSAFCYKDGQLIGVESVNRAADHVFGRKILPLGKSVTPEQAADLSFDL
KKAMA

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=373, Percent_Identity=31.3672922252011, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI226437568, Length=373, Percent_Identity=31.3672922252011, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI21389617, Length=373, Percent_Identity=31.3672922252011, Blast_Score=183, Evalue=3e-46,
Organism=Homo sapiens, GI22202629, Length=345, Percent_Identity=26.3768115942029, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI4757732, Length=345, Percent_Identity=26.3768115942029, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1788892, Length=403, Percent_Identity=35.7320099255583, Blast_Score=205, Evalue=4e-54,
Organism=Escherichia coli, GI1789765, Length=264, Percent_Identity=29.9242424242424, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789065, Length=282, Percent_Identity=27.6595744680851, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI87082354, Length=260, Percent_Identity=26.1538461538462, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17559934, Length=411, Percent_Identity=24.8175182481752, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24639257, Length=361, Percent_Identity=27.4238227146814, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI281359715, Length=361, Percent_Identity=27.4238227146814, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI281359713, Length=361, Percent_Identity=27.4238227146814, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24639250, Length=361, Percent_Identity=27.4238227146814, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI18543267, Length=361, Percent_Identity=27.4238227146814, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24639252, Length=361, Percent_Identity=27.4238227146814, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24581020, Length=362, Percent_Identity=28.1767955801105, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI28573993, Length=361, Percent_Identity=27.4238227146814, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24585130, Length=374, Percent_Identity=25.4010695187166, Blast_Score=105, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3

Molecular weight: Translated: 43664; Mature: 43664

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDTVLIAGAGHAGFQVAVSLRQAKYTGRIALINDEKHLPYQRPPLSKAYLKSGGDPNSL
CCCEEEEEECCCCCEEEEEEEEHHHCCCEEEEEECCCCCCCCCCCCCHHHHHCCCCCCCE
MFRPEKFFQDQTIELIDGRAVAIDRDAKTLLLASGDKIEYGHLVLATGARNRQLDVPNAT
EECCHHHCCCCCEEEECCEEEEEECCCCEEEEECCCEEEECEEEEEECCCCCEEECCCCC
LDDVLYLRTLDESEVVRQRMPEKKHVVVIGAGFIGLEFAATARGKGMEVDVVELAPRVMA
HHHEEEEEECCHHHHHHHHCCCCCEEEEEECCEEEEEEEEECCCCCCEEEHHHHHHHHHH
RAVTPEISSYFHDRHTAAGIRIHYGVRATEIEGEDGRVTGVALSDGRTLPCDLVVVGVGV
HHCCHHHHHHHHHCCCCCCEEEEECEEEEEECCCCCEEEEEEECCCCCCCEEEEEEECCC
IPNVELASAAGLPTAAGIIVNEQLLTEDPNISAIGDCALFNSVRFGEVMRVESVQNATDQ
CCCCHHHHCCCCCCCCEEEEEHHHHCCCCCCCEEECHHHHCCCCCCCEEEHHHHHCCCHH
ARCVAARLTGSPATYDGYPWFWSDQGDDKLQIAGLTAGFDQVVLRGSVAERSFSAFCYKD
HEEEEEEECCCCCCCCCCCEEECCCCCCEEEEEEEECCHHHHHEECCHHHCCCEEEEEEC
GQLIGVESVNRAADHVFGRKILPLGKSVTPEQAADLSFDLKKAMA
CCEEEHHHHHHHHHHHHCCEEECCCCCCCHHHHCCCCHHHHHHCC
>Mature Secondary Structure
MNDTVLIAGAGHAGFQVAVSLRQAKYTGRIALINDEKHLPYQRPPLSKAYLKSGGDPNSL
CCCEEEEEECCCCCEEEEEEEEHHHCCCEEEEEECCCCCCCCCCCCCHHHHHCCCCCCCE
MFRPEKFFQDQTIELIDGRAVAIDRDAKTLLLASGDKIEYGHLVLATGARNRQLDVPNAT
EECCHHHCCCCCEEEECCEEEEEECCCCEEEEECCCEEEECEEEEEECCCCCEEECCCCC
LDDVLYLRTLDESEVVRQRMPEKKHVVVIGAGFIGLEFAATARGKGMEVDVVELAPRVMA
HHHEEEEEECCHHHHHHHHCCCCCEEEEEECCEEEEEEEEECCCCCCEEEHHHHHHHHHH
RAVTPEISSYFHDRHTAAGIRIHYGVRATEIEGEDGRVTGVALSDGRTLPCDLVVVGVGV
HHCCHHHHHHHHHCCCCCCEEEEECEEEEEECCCCCEEEEEEECCCCCCCEEEEEEECCC
IPNVELASAAGLPTAAGIIVNEQLLTEDPNISAIGDCALFNSVRFGEVMRVESVQNATDQ
CCCCHHHHCCCCCCCCEEEEEHHHHCCCCCCCEEECHHHHCCCCCCCEEEHHHHHCCCHH
ARCVAARLTGSPATYDGYPWFWSDQGDDKLQIAGLTAGFDQVVLRGSVAERSFSAFCYKD
HEEEEEEECCCCCCCCCCCEEECCCCCCEEEEEEEECCHHHHHEECCHHHCCCEEEEEEC
GQLIGVESVNRAADHVFGRKILPLGKSVTPEQAADLSFDLKKAMA
CCEEEHHHHHHHHHHHHCCEEECCCCCCCHHHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced ferredoxin; NAD+

Specific reaction: reduced ferredoxin + NAD+ = oxidized ferredoxin + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]