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The map label for this gene is livM [H]

Identifier: 86750728

GI number: 86750728

Start: 4153100

End: 4154086

Strand: Reverse

Name: livM [H]

Synonym: RPB_3618

Alternate gene names: 86750728

Gene position: 4154086-4153100 (Counterclockwise)

Preceding gene: 86750729

Following gene: 86750727

Centisome position: 77.91

GC content: 64.03

Gene sequence:

>987_bases
ATGGACGAGGCTCATCTCAAGCGCCGCCGCCGCGATCTCATCGTCGCCACCGTGCTCGCCTGTATCGCGGCGCTGGTGCC
GCTGTTCGTCAAGGACGTCTACGTCCAGAACATCATGGTCTTGACCTTGATGTACGCAGCGCTGTCGCAGGCCTGGAACA
TCCTCGGCGGCTATTGCGGCCAGATCTCGCTGGGCCATGCGCTGTATTTCGGGCTCGGGGCCTATACGACCGCGCTGCTG
TTCACCAAGTTCGGCGTGCTGCCGTGGTTCGGGATGCTCGGTGGTGGCCTGATCTCGGCGGCGATCGCGCTGGCGCTCGG
CTATCCGACCTTCCGGCTGCGCGGGCATTATTTCGTCATCGCCACCATCGTGATCGCCGAAATCGGCTTCCTGCTGTTCC
ACAATTGGGACTGGGCGGGCGCCGCGCTCGGCATCGACATCCCGGTGCGCGGCGACAGTTGGGCCAAATTCCAGTTCACC
CGCAGCAAACTGCCTTATTACTACTTCGCCCTGGTGTTCTGCTGCGTCGCCTGGCTGGTGACGTGGTGGCTGGAGAATTC
GAAATGGGGCTATTGGTGGCGCGCCGTGAAGGACAATCCCGAGGCTGCTGAAAGCCTCGGCGTGGTGGTATTCAACTCCA
AGATGGGCGCTGCCGCGGTGTCGGCGTTCCTCACCGCGATCGGCGGCGCGTTCTACGCGATGTTCGTGTCCTACATCGAC
CCGGAAAGCGTGATGACCTTCCAGTTCTCGCTGCTGATGGCGCTGCCCGCGGTGCTGGGCGGCATCGGCACGCTGTGGGG
GCCGGTGCTCGGCGCAGTGATCCTGATCCCGCTCACCGAACTGACTCGCTCCTTCGTCGGCGGCTCCGGCCGCGGCGTCG
ATCTGATCCTCTACGGCACCGTGATCGTGCTGATTTCGCTGGCGCGCCCCGAAGGCCTGATCGGATTGTTCTCGCGGCGA
CCTGCCTCGAAGGGAGTGCCGCAATGA

Upstream 100 bases:

>100_bases
CCGCGCTGCTGTTGCCGCCATCGCTGAAGGCGGTCGGCATCTACGCGGTGTACCTGCTCGTTGTCTTCATTCGGCCCCGT
GGCCTGTTCGGATCGATCTG

Downstream 100 bases:

>100_bases
CCGCGCCACTGCTCGAGACCCGCGGCGTCTGGCAGCGTTTCGGCGGCCTGATCGCCAACAGCGACGTGTCGATCTCGGTC
GACCGCGGCGAGATCGTCGG

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MDEAHLKRRRRDLIVATVLACIAALVPLFVKDVYVQNIMVLTLMYAALSQAWNILGGYCGQISLGHALYFGLGAYTTALL
FTKFGVLPWFGMLGGGLISAAIALALGYPTFRLRGHYFVIATIVIAEIGFLLFHNWDWAGAALGIDIPVRGDSWAKFQFT
RSKLPYYYFALVFCCVAWLVTWWLENSKWGYWWRAVKDNPEAAESLGVVVFNSKMGAAAVSAFLTAIGGAFYAMFVSYID
PESVMTFQFSLLMALPAVLGGIGTLWGPVLGAVILIPLTELTRSFVGGSGRGVDLILYGTVIVLISLARPEGLIGLFSRR
PASKGVPQ

Sequences:

>Translated_328_residues
MDEAHLKRRRRDLIVATVLACIAALVPLFVKDVYVQNIMVLTLMYAALSQAWNILGGYCGQISLGHALYFGLGAYTTALL
FTKFGVLPWFGMLGGGLISAAIALALGYPTFRLRGHYFVIATIVIAEIGFLLFHNWDWAGAALGIDIPVRGDSWAKFQFT
RSKLPYYYFALVFCCVAWLVTWWLENSKWGYWWRAVKDNPEAAESLGVVVFNSKMGAAAVSAFLTAIGGAFYAMFVSYID
PESVMTFQFSLLMALPAVLGGIGTLWGPVLGAVILIPLTELTRSFVGGSGRGVDLILYGTVIVLISLARPEGLIGLFSRR
PASKGVPQ
>Mature_328_residues
MDEAHLKRRRRDLIVATVLACIAALVPLFVKDVYVQNIMVLTLMYAALSQAWNILGGYCGQISLGHALYFGLGAYTTALL
FTKFGVLPWFGMLGGGLISAAIALALGYPTFRLRGHYFVIATIVIAEIGFLLFHNWDWAGAALGIDIPVRGDSWAKFQFT
RSKLPYYYFALVFCCVAWLVTWWLENSKWGYWWRAVKDNPEAAESLGVVVFNSKMGAAAVSAFLTAIGGAFYAMFVSYID
PESVMTFQFSLLMALPAVLGGIGTLWGPVLGAVILIPLTELTRSFVGGSGRGVDLILYGTVIVLISLARPEGLIGLFSRR
PASKGVPQ

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=311, Percent_Identity=27.9742765273312, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 35802; Mature: 35802

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEAHLKRRRRDLIVATVLACIAALVPLFVKDVYVQNIMVLTLMYAALSQAWNILGGYCG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
QISLGHALYFGLGAYTTALLFTKFGVLPWFGMLGGGLISAAIALALGYPTFRLRGHYFVI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHH
ATIVIAEIGFLLFHNWDWAGAALGIDIPVRGDSWAKFQFTRSKLPYYYFALVFCCVAWLV
HHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEEEEHHHCCCHHHHHHHHHHHHHHH
TWWLENSKWGYWWRAVKDNPEAAESLGVVVFNSKMGAAAVSAFLTAIGGAFYAMFVSYID
HHHHCCCCCCEEEEEECCCHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCC
PESVMTFQFSLLMALPAVLGGIGTLWGPVLGAVILIPLTELTRSFVGGSGRGVDLILYGT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHH
VIVLISLARPEGLIGLFSRRPASKGVPQ
HHHHHHHCCCCCHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MDEAHLKRRRRDLIVATVLACIAALVPLFVKDVYVQNIMVLTLMYAALSQAWNILGGYCG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
QISLGHALYFGLGAYTTALLFTKFGVLPWFGMLGGGLISAAIALALGYPTFRLRGHYFVI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHH
ATIVIAEIGFLLFHNWDWAGAALGIDIPVRGDSWAKFQFTRSKLPYYYFALVFCCVAWLV
HHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEEEEHHHCCCHHHHHHHHHHHHHHH
TWWLENSKWGYWWRAVKDNPEAAESLGVVVFNSKMGAAAVSAFLTAIGGAFYAMFVSYID
HHHHCCCCCCEEEEEECCCHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCC
PESVMTFQFSLLMALPAVLGGIGTLWGPVLGAVILIPLTELTRSFVGGSGRGVDLILYGT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHH
VIVLISLARPEGLIGLFSRRPASKGVPQ
HHHHHHHCCCCCHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]