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The map label for this gene is caiD [C]

Identifier: 86750708

GI number: 86750708

Start: 4128301

End: 4129113

Strand: Reverse

Name: caiD [C]

Synonym: RPB_3598

Alternate gene names: 86750708

Gene position: 4129113-4128301 (Counterclockwise)

Preceding gene: 86750711

Following gene: 86750707

Centisome position: 77.45

GC content: 68.39

Gene sequence:

>813_bases
ATGAATGATCGCGTTGCGGTATCGGTGACGGACGGCGTTGCCGACGTCCGATTGGTGCGGGCGGACAAGATGAATGCGCT
CGATGCCGCGATGTTCGAGGCGCTTGTCGCGACCACCGAGCGGCTTTCGCAGGAGAAGGGCGTGCGCGTCGTGGTGCTGT
CCGGCGAGGGCAAGGCGTTCTGCGCCGGCCTCGACATGGGGCGTTTTGCCGCGATGAACGAGGGCGGCGGCAACGGCATT
CCGGGCGGCGAATTTCGCGATCTCACCAAGCGCACCCATGGCCAGGCCAACGCGCCGCAGCAGGCGGTGTGGGGCTGGCG
GATGCTGCCGGTGCCGGTGATCGCCGCGATCCACGGCGTCGCGTTCGGCGGCGGCTTTCAGCTCGCGCTCGGCGCCGACA
TCCGGCTGATGGCGCCGGATGCGCGGATGTCGATCATGGAAATCAAATGGGGCCTGGTGCCCGACATGGCCGGCACGCCG
GTGCTCGCCTCGCTGGTGCGCGACGACATCCTGCGCGAGCTCACCTACACCGGCCGGATCTTCTCGGCGCAGGAGGCGCT
GAGCTACGGCCTCGCGACCAGGATCGTCGACGACCCGCGCAGCGCCGCGCTGGAGATGGCGCGGGAGATCGCCGGCAAGA
GCCCGGACGCCATCCGCGCCGCCAAGCGGATGTTCAACAACCTCTCGGTCGACCCCGGCCCTGCGCTGCTCGCCGAAAGC
GTCGAACAGCAGAAGCTGATCGGCTCGCCCAACCAGACCGAAGCGGTCCGCGCCAACATGGAAAAGCGCGCGCCCAATTT
CGTCGACGGTTAG

Upstream 100 bases:

>100_bases
AAAGTGGATGATCGTTGTTGATTAAGCGATTGACTAAATTCCGACAACGCTTTTCTGTAGCGCCGCGAACAGATCACAAC
ATATCCAGGGAAACGGCTCG

Downstream 100 bases:

>100_bases
TTACACCTTCGGCCGTCATCCTGAGGTGCTCGCCCGCAAGGGCGAGCCTCGAAGGATGCGATACCTGTACCTGCGGCTCA
TCCTTCGAGGCTCGCTTCGC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MNDRVAVSVTDGVADVRLVRADKMNALDAAMFEALVATTERLSQEKGVRVVVLSGEGKAFCAGLDMGRFAAMNEGGGNGI
PGGEFRDLTKRTHGQANAPQQAVWGWRMLPVPVIAAIHGVAFGGGFQLALGADIRLMAPDARMSIMEIKWGLVPDMAGTP
VLASLVRDDILRELTYTGRIFSAQEALSYGLATRIVDDPRSAALEMAREIAGKSPDAIRAAKRMFNNLSVDPGPALLAES
VEQQKLIGSPNQTEAVRANMEKRAPNFVDG

Sequences:

>Translated_270_residues
MNDRVAVSVTDGVADVRLVRADKMNALDAAMFEALVATTERLSQEKGVRVVVLSGEGKAFCAGLDMGRFAAMNEGGGNGI
PGGEFRDLTKRTHGQANAPQQAVWGWRMLPVPVIAAIHGVAFGGGFQLALGADIRLMAPDARMSIMEIKWGLVPDMAGTP
VLASLVRDDILRELTYTGRIFSAQEALSYGLATRIVDDPRSAALEMAREIAGKSPDAIRAAKRMFNNLSVDPGPALLAES
VEQQKLIGSPNQTEAVRANMEKRAPNFVDG
>Mature_270_residues
MNDRVAVSVTDGVADVRLVRADKMNALDAAMFEALVATTERLSQEKGVRVVVLSGEGKAFCAGLDMGRFAAMNEGGGNGI
PGGEFRDLTKRTHGQANAPQQAVWGWRMLPVPVIAAIHGVAFGGGFQLALGADIRLMAPDARMSIMEIKWGLVPDMAGTP
VLASLVRDDILRELTYTGRIFSAQEALSYGLATRIVDDPRSAALEMAREIAGKSPDAIRAAKRMFNNLSVDPGPALLAES
VEQQKLIGSPNQTEAVRANMEKRAPNFVDG

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI70995211, Length=222, Percent_Identity=32.8828828828829, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI4502327, Length=264, Percent_Identity=29.1666666666667, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI194097323, Length=266, Percent_Identity=28.5714285714286, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI31542718, Length=258, Percent_Identity=26.7441860465116, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI68989263, Length=221, Percent_Identity=26.2443438914027, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI221142681, Length=235, Percent_Identity=31.4893617021277, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787659, Length=265, Percent_Identity=27.9245283018868, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788682, Length=214, Percent_Identity=32.2429906542056, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87082183, Length=279, Percent_Identity=25.4480286738351, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787660, Length=271, Percent_Identity=29.520295202952, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790281, Length=188, Percent_Identity=27.1276595744681, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788597, Length=262, Percent_Identity=25.9541984732824, Blast_Score=68, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17536985, Length=220, Percent_Identity=33.1818181818182, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17540714, Length=240, Percent_Identity=30.8333333333333, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17534483, Length=225, Percent_Identity=29.7777777777778, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI25145438, Length=275, Percent_Identity=28.7272727272727, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17554946, Length=260, Percent_Identity=28.4615384615385, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17560910, Length=219, Percent_Identity=27.3972602739726, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17558304, Length=207, Percent_Identity=26.0869565217391, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17508951, Length=221, Percent_Identity=29.4117647058824, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25144276, Length=221, Percent_Identity=29.4117647058824, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17508953, Length=221, Percent_Identity=29.4117647058824, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24653139, Length=264, Percent_Identity=30.3030303030303, Blast_Score=107, Evalue=7e-24,
Organism=Drosophila melanogaster, GI19920382, Length=218, Percent_Identity=32.1100917431193, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI20129971, Length=260, Percent_Identity=26.9230769230769, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24653477, Length=260, Percent_Identity=26.9230769230769, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28803; Mature: 28803

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDRVAVSVTDGVADVRLVRADKMNALDAAMFEALVATTERLSQEKGVRVVVLSGEGKAF
CCCCEEEEEECCCHHEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEE
CAGLDMGRFAAMNEGGGNGIPGGEFRDLTKRTHGQANAPQQAVWGWRMLPVPVIAAIHGV
EECCCCCHHEECCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHHHH
AFGGGFQLALGADIRLMAPDARMSIMEIKWGLVPDMAGTPVLASLVRDDILRELTYTGRI
HCCCCEEEEECCCEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCH
FSAQEALSYGLATRIVDDPRSAALEMAREIAGKSPDAIRAAKRMFNNLSVDPGPALLAES
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHH
VEQQKLIGSPNQTEAVRANMEKRAPNFVDG
HHHHHHCCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNDRVAVSVTDGVADVRLVRADKMNALDAAMFEALVATTERLSQEKGVRVVVLSGEGKAF
CCCCEEEEEECCCHHEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEE
CAGLDMGRFAAMNEGGGNGIPGGEFRDLTKRTHGQANAPQQAVWGWRMLPVPVIAAIHGV
EECCCCCHHEECCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHHHH
AFGGGFQLALGADIRLMAPDARMSIMEIKWGLVPDMAGTPVLASLVRDDILRELTYTGRI
HCCCCEEEEECCCEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCH
FSAQEALSYGLATRIVDDPRSAALEMAREIAGKSPDAIRAAKRMFNNLSVDPGPALLAES
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHH
VEQQKLIGSPNQTEAVRANMEKRAPNFVDG
HHHHHHCCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA