Click here to switch to the map view.

The map label for this gene is mutB [H]

Identifier: 86750648

GI number: 86750648

Start: 4049217

End: 4051427

Strand: Direct

Name: mutB [H]

Synonym: RPB_3538

Alternate gene names: 86750648

Gene position: 4049217-4051427 (Clockwise)

Preceding gene: 86750647

Following gene: 86750649

Centisome position: 75.95

GC content: 65.67

Gene sequence:

>2211_bases
ATGACGCCCAACAGCGGCCGTGCTAAAATGAGCCGCGCAGAGATTGAGCCAATGAGCAAGATTCCGAACTTCGCCGAGAT
CGCGTTCCAGCCCACCGCCGCCCCTGCGCCCGCAGGCGAGGCCAAGCCGTGGCTGACGCCGGAAGGCATCCTGGTCAAGC
CGGGCTACAGCGAAGCCGATCTCGACGGCATCGACTTCCTCGATACATATCCCGGCATCGCGCCGTTCCTGCGCGGCCCC
TACCCCACGATGTACGTCAACCAGCCGTGGACGATCCGGCAATATGCCGGCTTCTCGACCGCGGAGGATTCCAACGCATT
TTATCGCCGCAATCTGGCCGCCGGCCAGAAGGGCCTGTCGGTGGCGTTCGACCTCGCCACCCATCGCGGCTACGACTCTG
ATCATCCGCGCGTCGCCGGCGACGTCGGCATGGCGGGCGTGGCGATCGACAGCATCTACGACATGCGCACGCTGTTCGCC
GGCATCCCGCTCGACCAGATGAGCGTGTCGATGACCATGAACGGCGCGGTGCTGCCGATCCTCGCTCTGTTCGTCGCCGC
CGCCGAAGAACAGGGCGTGCCGCCGGAGAAGCTGTCGGGCACCATTCAGAACGACATTCTGAAAGAGTTCATGGTGCGCA
ACACCTACATCTATCCGCCGACGCCGTCGATGCGGGTGATTTCCGACATCTTCGCCTACACCTCGCAGAAGATGCCGAAG
TACAACTCGATCTCGATCTCCGGCTATCACATGCAGGAAGCCGGCGCGACGCAGGACCTCGAGCTCGCTTATACGCTGGC
CGATGGCGTCGAATATCTCCGCGCCGGCATCGCCGCCGGCCTCGACGTCGACCGCTTCGCACCGCGGCTGTCGTTCTTCT
GGGCGATCGGCATGAACTTCTTCATGGAAGTCGCCAAGATGCGCGCCGCCCGGCTGCTGTGGGCCAAGCTGCTGACGCAG
TTCAATCCGAAGGACCCGAAGTCGCTCAGCCTGCGCACGCATTGCCAGACCTCCGGCTGGTCGCTGACCGCGCAGGATGT
CTACAACAACGTGATGCGCACCACGATCGAGGCGATGGCGGCGACCCAGGGTCATACGCAATCGCTGCACACCAATGCGC
TCGACGAGGCGCTGGCGCTGCCGACCGACTTCTCCGCGCGGATCGCGCGCAACACGCAATTGTTCCTGCAACAAGAGACC
GGCACCACCCGGATCATCGATCCGTGGGGCGGCTCGTACTACGTCGAACGCCTGACCCACGATCTCGCCGTCAAGGCGTG
GGGCCATATCCAGGAGGTCGAGGCACTCGGCGGCATGGCCAAGGCGATCGAAGCCGGCGTGCCGAAACTGCGCATCGAGG
AAGCCTCGGCCAAGACCCAGGCGCGGATCGACACCGGCCGGCAATCGGTGATCGGCGTCAACAAATACAAACCGACCGAC
GAAGCGCCGCTCGACGTGCTCAAGGTCGAGAATTCGACCGTGCGGCGGCTGCAGATCGACAAGCTGAAGCGGCTGCGTGG
CGACCGCAGCCAGGCCGACGTCGACGCAGCGCTCGCGGCGCTGACGCGCTCGGCTGCGGACGGCAACGGCAATCTGCTGG
CGCTGGCGATCGACGCGGCGCGGGCCAAGGCCACCGTCGGCGAAATCTCCGACGCGCTGGAGAAGGTCTATGGCCGGCAT
CGCGCCGAGATCCGGTCGATCACCGGCGTCTACAAGCGGGAGGTCGCGAGCATGGCGAACCGGATGGAGAAGGTGCAGGC
GCTGATCGACGCGTTCGAGGACGCCGAGGGCCGCCGCCCGCGCATTCTCGTCGCCAAGATCGGCCAGGACGGCCACGATC
GCGGCCAGAAGGTGATCGCCTCGGCGTTCGCCGACATCGGCTTCGACGTCGATATCGGGCCCTTGTTCGCAACCGCCGAT
GAGGCTGCACGGCAGGCGGTCGAGAACGACGTCCACATTCTCGGCGTGTCGTCGCTCGCCGCCGCCCACCTCACCGCGGT
GCCGGAGCTGAAGGCGGCGCTGAAGAAGCAGGGTCGCGAGGACATCATGATCATCATCGGCGGCGTGGTGCCGCCGCAGG
ACTACGACGCGCTGATCAAATCCGGCGCCGAGGCGATCTTCCCGCCCGGCACGGTGATCGCCGACGCCGCCGAGGAACTG
ATCCACAAGCTCAACGCAAGGCTCGGGCATTCGAGCCAGGCGGCGGAGTAG

Upstream 100 bases:

>100_bases
GCGAACCTGCCGAGCAAGACGACCACGCAGATCTACGGACCCGAGAAGCTGGCGCAGGTGTTCAGCTATCAGCAGCCGGA
TCACGATTGAACCCGACGAA

Downstream 100 bases:

>100_bases
CTGCGGGGGACGGCAACCTTGTGCTAGGATGCACGAATGACAGCCATTGCCGACATGCCGCTGGACAAGATCGTCGCGAA
GCTGGTCGAGCTCACGCCTG

Product: methylmalonyl-CoA mutase

Products: NA

Alternate protein names: MCM-alpha [H]

Number of amino acids: Translated: 736; Mature: 735

Protein sequence:

>736_residues
MTPNSGRAKMSRAEIEPMSKIPNFAEIAFQPTAAPAPAGEAKPWLTPEGILVKPGYSEADLDGIDFLDTYPGIAPFLRGP
YPTMYVNQPWTIRQYAGFSTAEDSNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRVAGDVGMAGVAIDSIYDMRTLFA
GIPLDQMSVSMTMNGAVLPILALFVAAAEEQGVPPEKLSGTIQNDILKEFMVRNTYIYPPTPSMRVISDIFAYTSQKMPK
YNSISISGYHMQEAGATQDLELAYTLADGVEYLRAGIAAGLDVDRFAPRLSFFWAIGMNFFMEVAKMRAARLLWAKLLTQ
FNPKDPKSLSLRTHCQTSGWSLTAQDVYNNVMRTTIEAMAATQGHTQSLHTNALDEALALPTDFSARIARNTQLFLQQET
GTTRIIDPWGGSYYVERLTHDLAVKAWGHIQEVEALGGMAKAIEAGVPKLRIEEASAKTQARIDTGRQSVIGVNKYKPTD
EAPLDVLKVENSTVRRLQIDKLKRLRGDRSQADVDAALAALTRSAADGNGNLLALAIDAARAKATVGEISDALEKVYGRH
RAEIRSITGVYKREVASMANRMEKVQALIDAFEDAEGRRPRILVAKIGQDGHDRGQKVIASAFADIGFDVDIGPLFATAD
EAARQAVENDVHILGVSSLAAAHLTAVPELKAALKKQGREDIMIIIGGVVPPQDYDALIKSGAEAIFPPGTVIADAAEEL
IHKLNARLGHSSQAAE

Sequences:

>Translated_736_residues
MTPNSGRAKMSRAEIEPMSKIPNFAEIAFQPTAAPAPAGEAKPWLTPEGILVKPGYSEADLDGIDFLDTYPGIAPFLRGP
YPTMYVNQPWTIRQYAGFSTAEDSNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRVAGDVGMAGVAIDSIYDMRTLFA
GIPLDQMSVSMTMNGAVLPILALFVAAAEEQGVPPEKLSGTIQNDILKEFMVRNTYIYPPTPSMRVISDIFAYTSQKMPK
YNSISISGYHMQEAGATQDLELAYTLADGVEYLRAGIAAGLDVDRFAPRLSFFWAIGMNFFMEVAKMRAARLLWAKLLTQ
FNPKDPKSLSLRTHCQTSGWSLTAQDVYNNVMRTTIEAMAATQGHTQSLHTNALDEALALPTDFSARIARNTQLFLQQET
GTTRIIDPWGGSYYVERLTHDLAVKAWGHIQEVEALGGMAKAIEAGVPKLRIEEASAKTQARIDTGRQSVIGVNKYKPTD
EAPLDVLKVENSTVRRLQIDKLKRLRGDRSQADVDAALAALTRSAADGNGNLLALAIDAARAKATVGEISDALEKVYGRH
RAEIRSITGVYKREVASMANRMEKVQALIDAFEDAEGRRPRILVAKIGQDGHDRGQKVIASAFADIGFDVDIGPLFATAD
EAARQAVENDVHILGVSSLAAAHLTAVPELKAALKKQGREDIMIIIGGVVPPQDYDALIKSGAEAIFPPGTVIADAAEEL
IHKLNARLGHSSQAAE
>Mature_735_residues
TPNSGRAKMSRAEIEPMSKIPNFAEIAFQPTAAPAPAGEAKPWLTPEGILVKPGYSEADLDGIDFLDTYPGIAPFLRGPY
PTMYVNQPWTIRQYAGFSTAEDSNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRVAGDVGMAGVAIDSIYDMRTLFAG
IPLDQMSVSMTMNGAVLPILALFVAAAEEQGVPPEKLSGTIQNDILKEFMVRNTYIYPPTPSMRVISDIFAYTSQKMPKY
NSISISGYHMQEAGATQDLELAYTLADGVEYLRAGIAAGLDVDRFAPRLSFFWAIGMNFFMEVAKMRAARLLWAKLLTQF
NPKDPKSLSLRTHCQTSGWSLTAQDVYNNVMRTTIEAMAATQGHTQSLHTNALDEALALPTDFSARIARNTQLFLQQETG
TTRIIDPWGGSYYVERLTHDLAVKAWGHIQEVEALGGMAKAIEAGVPKLRIEEASAKTQARIDTGRQSVIGVNKYKPTDE
APLDVLKVENSTVRRLQIDKLKRLRGDRSQADVDAALAALTRSAADGNGNLLALAIDAARAKATVGEISDALEKVYGRHR
AEIRSITGVYKREVASMANRMEKVQALIDAFEDAEGRRPRILVAKIGQDGHDRGQKVIASAFADIGFDVDIGPLFATADE
AARQAVENDVHILGVSSLAAAHLTAVPELKAALKKQGREDIMIIIGGVVPPQDYDALIKSGAEAIFPPGTVIADAAEELI
HKLNARLGHSSQAAE

Specific function: Catalyzes the isomerization of succinyl-CoA to methylmalonyl-CoA during synthesis of propionate from tricarboxylic acid-cycle intermediates. This conversion most likely represents an important source of building blocks for polyketide antibiotic biosynthes

COG id: COG1884

COG function: function code I; Methylmalonyl-CoA mutase, N-terminal domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156105689, Length=714, Percent_Identity=60.3641456582633, Blast_Score=900, Evalue=0.0,
Organism=Escherichia coli, GI1789284, Length=683, Percent_Identity=61.7862371888726, Blast_Score=850, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25144500, Length=688, Percent_Identity=61.9186046511628, Blast_Score=884, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006159
- InterPro:   IPR016176
- InterPro:   IPR014348
- InterPro:   IPR006158
- InterPro:   IPR006099
- InterPro:   IPR006098 [H]

Pfam domain/function: PF02310 B12-binding; PF01642 MM_CoA_mutase [H]

EC number: =5.4.99.2 [H]

Molecular weight: Translated: 79916; Mature: 79785

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00544 METMALONYL_COA_MUTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPNSGRAKMSRAEIEPMSKIPNFAEIAFQPTAAPAPAGEAKPWLTPEGILVKPGYSEAD
CCCCCCCCHHHHHHCCHHHHCCCHHHEEECCCCCCCCCCCCCCCCCCCCEEECCCCCCCC
LDGIDFLDTYPGIAPFLRGPYPTMYVNQPWTIRQYAGFSTAEDSNAFYRRNLAAGQKGLS
CCCCCHHHCCCCHHHHHCCCCCEEEECCCEEEHHHCCCCCCCCCCHHHHHHHHCCCCCCE
VAFDLATHRGYDSDHPRVAGDVGMAGVAIDSIYDMRTLFAGIPLDQMSVSMTMNGAVLPI
EEEEHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHCEEEEEECCHHHHH
LALFVAAAEEQGVPPEKLSGTIQNDILKEFMVRNTYIYPPTPSMRVISDIFAYTSQKMPK
HHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHCCCC
YNSISISGYHMQEAGATQDLELAYTLADGVEYLRAGIAAGLDVDRFAPRLSFFWAIGMNF
CCCEEEECEEECCCCCCCCHHEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
FMEVAKMRAARLLWAKLLTQFNPKDPKSLSLRTHCQTSGWSLTAQDVYNNVMRTTIEAMA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEEHHHHHHHHHHHHHHHHH
ATQGHTQSLHTNALDEALALPTDFSARIARNTQLFLQQETGTTRIIDPWGGSYYVERLTH
HCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCEEEEEECCCCEEEECCCCCHHHHHHHHH
DLAVKAWGHIQEVEALGGMAKAIEAGVPKLRIEEASAKTQARIDTGRQSVIGVNKYKPTD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHCCHHHHHHHCCCHHEEECCCCCCCC
EAPLDVLKVENSTVRRLQIDKLKRLRGDRSQADVDAALAALTRSAADGNGNLLALAIDAA
CCCCEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECH
RAKATVGEISDALEKVYGRHRAEIRSITGVYKREVASMANRMEKVQALIDAFEDAEGRRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RILVAKIGQDGHDRGQKVIASAFADIGFDVDIGPLFATADEAARQAVENDVHILGVSSLA
EEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEHHHHH
AAHLTAVPELKAALKKQGREDIMIIIGGVVPPQDYDALIKSGAEAIFPPGTVIADAAEEL
HHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHH
IHKLNARLGHSSQAAE
HHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TPNSGRAKMSRAEIEPMSKIPNFAEIAFQPTAAPAPAGEAKPWLTPEGILVKPGYSEAD
CCCCCCCHHHHHHCCHHHHCCCHHHEEECCCCCCCCCCCCCCCCCCCCEEECCCCCCCC
LDGIDFLDTYPGIAPFLRGPYPTMYVNQPWTIRQYAGFSTAEDSNAFYRRNLAAGQKGLS
CCCCCHHHCCCCHHHHHCCCCCEEEECCCEEEHHHCCCCCCCCCCHHHHHHHHCCCCCCE
VAFDLATHRGYDSDHPRVAGDVGMAGVAIDSIYDMRTLFAGIPLDQMSVSMTMNGAVLPI
EEEEHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHCEEEEEECCHHHHH
LALFVAAAEEQGVPPEKLSGTIQNDILKEFMVRNTYIYPPTPSMRVISDIFAYTSQKMPK
HHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHCCCC
YNSISISGYHMQEAGATQDLELAYTLADGVEYLRAGIAAGLDVDRFAPRLSFFWAIGMNF
CCCEEEECEEECCCCCCCCHHEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
FMEVAKMRAARLLWAKLLTQFNPKDPKSLSLRTHCQTSGWSLTAQDVYNNVMRTTIEAMA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEEHHHHHHHHHHHHHHHHH
ATQGHTQSLHTNALDEALALPTDFSARIARNTQLFLQQETGTTRIIDPWGGSYYVERLTH
HCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCEEEEEECCCCEEEECCCCCHHHHHHHHH
DLAVKAWGHIQEVEALGGMAKAIEAGVPKLRIEEASAKTQARIDTGRQSVIGVNKYKPTD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHCCHHHHHHHCCCHHEEECCCCCCCC
EAPLDVLKVENSTVRRLQIDKLKRLRGDRSQADVDAALAALTRSAADGNGNLLALAIDAA
CCCCEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECH
RAKATVGEISDALEKVYGRHRAEIRSITGVYKREVASMANRMEKVQALIDAFEDAEGRRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RILVAKIGQDGHDRGQKVIASAFADIGFDVDIGPLFATADEAARQAVENDVHILGVSSLA
EEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEHHHHH
AAHLTAVPELKAALKKQGREDIMIIIGGVVPPQDYDALIKSGAEAIFPPGTVIADAAEEL
HHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHH
IHKLNARLGHSSQAAE
HHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8099072 [H]