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The map label for this gene is pbpF [H]

Identifier: 86750616

GI number: 86750616

Start: 4013583

End: 4015892

Strand: Direct

Name: pbpF [H]

Synonym: RPB_3506

Alternate gene names: 86750616

Gene position: 4013583-4015892 (Clockwise)

Preceding gene: 86750614

Following gene: 86750617

Centisome position: 75.28

GC content: 67.53

Gene sequence:

>2310_bases
ATGGAGGCGCCCTGCCCGGCGCCGTCACGCGATTGGTCGAAGCCGGTGCCGAAGATTCTGCCAGACAATTGGATGCAGCG
GATCCGGAACTGGTTTCTGGACCTCGACGCGCGGCTGGATTCGACGCTGTTCTCGTCGGCCGCCGGCATCCGCGAATTGT
GGGAGCGCTTCTCCACTTTCATGGACCGCTTCTATGTCGGTCACTGGAAGCGCTGGGTGTTCATCGAGCCGCTGTCGGAA
GCCGCCACGATGGGCGCCGGCGGCCTGCTGGTGCTGCTGGCGCTGGCGCAGCCGGCGTTCCGCGAGACCGGCGACGAGGA
CTGGCTGAAGAAATCCGACCTCGCCGTCTCCTTCCTGGATCGCTACGGCAATCCGATCGGCAATCGCGGCATCAAGCACA
ACGACTCGATCCCGCTGGAAGACTTCCCCGACAATCTGATCAAGGCGACGCTCGCCACCGAGGACCGCCGCTTCTACGAT
CATTTCGGCATCGACGTCGCCGGCACCGCGCGCGCGCTGGTGACCAATGCGCAGGCCGGCGGCGTGCGCCAGGGCGGCTC
CTCGATCACCCAGCAGCTCGCCAAGAACCTGTTCCTGTCGAACGAGCGCACGCTGGAACGCAAGGTCAACGAGGCGTTTC
TGGCGATCTGGCTGGAGACCCGGCTGACCAAGAACGAGATCCTCAAGTTGTATCTCGATCGCGCCTATATGGGCGGCGGC
ACCTTCGGCGCCGACGGCGCGGCGCATTTCTACTTCAACAAATCGGTGCGCGACGTGAACCTCGCCGAGGCCGCGATGCT
GGCCGGGCTGTTCAAGGCGCCGACCAAATTCGCCCCGCACATCAACCTGCCCGCCGCGCGCGCCCGCGCCAACGTCGTGC
TCGACAATCTGGTCGACGCCGGCTTCATGACCGAGGGCCAGGTGTTCGGCGCGCGGCGCAATCCGGCCTCGGTGGTCGAC
CGCCGCGACGAGAATTCGCCGAACTATTATCTCGACTGGGCGTTCGAGGAAGTCCGCAAGATCGTCGAGACGTTTCCGAA
ATCCTACACCGAGCGCGTCTTCGTGGTGCGCACCACGATCGACATGAACGTGCAGCGCGCCGCCGAGGCCGCGGTCGAGA
ACCAGCTCCGCCAGTTCGGCCGCGACTATCATGCGACGCAGGCCGCCGCGGTGCTGTCGGATCTCGACGGCGGCGTCCGC
GCCATGGTCGGCGGCCGCGACTACGGCTCCAGCCAGTTCAACCGCGCGGTCGACGCCTATCGCCAGCCCGGCTCGTCGTT
CAAGCCGTATGTCTATACGACCGCGCTGCTCAACGGCTTCAAGCCGACCTCGATCGTGGTCGACGGCCCGGTCTGCATCG
GCAATTGGTGCCCGCAGAATTACGGCCACTCTTATTCGGGTTCGGTGACGCTGACCCAGGCGATCACCCGCTCGATCAAC
GTCGTGCCGGTGAAGCTGTCGATCACGCTCGGCGGCAAGGCCGGGCCGAAGGCCGGCCGCGCCAAGATCGTCGAAGTCGC
GCGCCGCTTCGGCATCAAGGCGCCGCTGATCGACACCCCGTCGCTGCCGATCGGCTCGACCGAAGTCACCGTGCTCGAAC
ACGCGGTGGCCTACGCCACCTTCCCGAACAAGGGCAAGGCGCCGACGCCGCACGCGGTGCTCGAAGTGCGCACCGGCGCC
GGCGATCTGGTGTGGCGGTTCGACCGCGACGGCCCGAAGCAGAAGCAGGCGATCCCGGCCTCGGTCGCCGCCGACATGGC
CGGCATGATGAGCCACGTCGTCTCCGAGGGCACCGCGCGCCGCGCAGCCCTCGACGGCATTCCGACCGCGGGCAAGACCG
GCACCACCAACGCCTATCGCGACGCCTGGTTCGTCGGCTACACCGGCAATTTCAGTTGCGCGGTGTGGTACGGCAACGAC
GACTACTCGCCGACCAACCGCATGACCGGCGGCTCGCTGCCGGCGCAGACCTGGCGCGACATCATGATCGCGGCGCATCA
GGGCGTCGAGATCAAGGAGCTCGCCGGCGTCGGCATGGGCACCAGGCTGCCGGCCTCGCAGGCGCAAGCCAATGCGCGCG
CCGCCGACGACGACGGCAAACCGGCGCCGCCACCGGTGCTGACGCGGCGCGGCGCGGATATTCTGGTGCGCGTCGAAAAG
ATGCTGGACGACGCCGCCAAGGCCATGGACAAGGCGATGGACAAGACCCCGCCCGCCAAGCCGGTGTCGTCGACCGGCGT
CGGATTCCCGGACAGTTTCGCCGCGGCGACGCCGAACGGGCCGCCGGCGACGGCGCCGCGCAAGAACTGA

Upstream 100 bases:

>100_bases
CGCGGCCATTGGTCTAAGGCCGCCGCTCGGCTACAAGGGGCGAGATTCCCTGGGATCGCCGCGCGCGCCTTGGGTTTGCC
GCAACGCTGCAGCAAGACGG

Downstream 100 bases:

>100_bases
CGGCGGCTCGTAGCGCAAGGACGGTTGCAACGTGCGCCTGATCTTCTTCACTTTGCTGGCGCTGATCATCGCCGCGGGCG
TCGGCGTCGGCGCGACCTGG

Product: penicillin-binding protein 1A

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 769; Mature: 769

Protein sequence:

>769_residues
MEAPCPAPSRDWSKPVPKILPDNWMQRIRNWFLDLDARLDSTLFSSAAGIRELWERFSTFMDRFYVGHWKRWVFIEPLSE
AATMGAGGLLVLLALAQPAFRETGDEDWLKKSDLAVSFLDRYGNPIGNRGIKHNDSIPLEDFPDNLIKATLATEDRRFYD
HFGIDVAGTARALVTNAQAGGVRQGGSSITQQLAKNLFLSNERTLERKVNEAFLAIWLETRLTKNEILKLYLDRAYMGGG
TFGADGAAHFYFNKSVRDVNLAEAAMLAGLFKAPTKFAPHINLPAARARANVVLDNLVDAGFMTEGQVFGARRNPASVVD
RRDENSPNYYLDWAFEEVRKIVETFPKSYTERVFVVRTTIDMNVQRAAEAAVENQLRQFGRDYHATQAAAVLSDLDGGVR
AMVGGRDYGSSQFNRAVDAYRQPGSSFKPYVYTTALLNGFKPTSIVVDGPVCIGNWCPQNYGHSYSGSVTLTQAITRSIN
VVPVKLSITLGGKAGPKAGRAKIVEVARRFGIKAPLIDTPSLPIGSTEVTVLEHAVAYATFPNKGKAPTPHAVLEVRTGA
GDLVWRFDRDGPKQKQAIPASVAADMAGMMSHVVSEGTARRAALDGIPTAGKTGTTNAYRDAWFVGYTGNFSCAVWYGND
DYSPTNRMTGGSLPAQTWRDIMIAAHQGVEIKELAGVGMGTRLPASQAQANARAADDDGKPAPPPVLTRRGADILVRVEK
MLDDAAKAMDKAMDKTPPAKPVSSTGVGFPDSFAAATPNGPPATAPRKN

Sequences:

>Translated_769_residues
MEAPCPAPSRDWSKPVPKILPDNWMQRIRNWFLDLDARLDSTLFSSAAGIRELWERFSTFMDRFYVGHWKRWVFIEPLSE
AATMGAGGLLVLLALAQPAFRETGDEDWLKKSDLAVSFLDRYGNPIGNRGIKHNDSIPLEDFPDNLIKATLATEDRRFYD
HFGIDVAGTARALVTNAQAGGVRQGGSSITQQLAKNLFLSNERTLERKVNEAFLAIWLETRLTKNEILKLYLDRAYMGGG
TFGADGAAHFYFNKSVRDVNLAEAAMLAGLFKAPTKFAPHINLPAARARANVVLDNLVDAGFMTEGQVFGARRNPASVVD
RRDENSPNYYLDWAFEEVRKIVETFPKSYTERVFVVRTTIDMNVQRAAEAAVENQLRQFGRDYHATQAAAVLSDLDGGVR
AMVGGRDYGSSQFNRAVDAYRQPGSSFKPYVYTTALLNGFKPTSIVVDGPVCIGNWCPQNYGHSYSGSVTLTQAITRSIN
VVPVKLSITLGGKAGPKAGRAKIVEVARRFGIKAPLIDTPSLPIGSTEVTVLEHAVAYATFPNKGKAPTPHAVLEVRTGA
GDLVWRFDRDGPKQKQAIPASVAADMAGMMSHVVSEGTARRAALDGIPTAGKTGTTNAYRDAWFVGYTGNFSCAVWYGND
DYSPTNRMTGGSLPAQTWRDIMIAAHQGVEIKELAGVGMGTRLPASQAQANARAADDDGKPAPPPVLTRRGADILVRVEK
MLDDAAKAMDKAMDKTPPAKPVSSTGVGFPDSFAAATPNGPPATAPRKN
>Mature_769_residues
MEAPCPAPSRDWSKPVPKILPDNWMQRIRNWFLDLDARLDSTLFSSAAGIRELWERFSTFMDRFYVGHWKRWVFIEPLSE
AATMGAGGLLVLLALAQPAFRETGDEDWLKKSDLAVSFLDRYGNPIGNRGIKHNDSIPLEDFPDNLIKATLATEDRRFYD
HFGIDVAGTARALVTNAQAGGVRQGGSSITQQLAKNLFLSNERTLERKVNEAFLAIWLETRLTKNEILKLYLDRAYMGGG
TFGADGAAHFYFNKSVRDVNLAEAAMLAGLFKAPTKFAPHINLPAARARANVVLDNLVDAGFMTEGQVFGARRNPASVVD
RRDENSPNYYLDWAFEEVRKIVETFPKSYTERVFVVRTTIDMNVQRAAEAAVENQLRQFGRDYHATQAAAVLSDLDGGVR
AMVGGRDYGSSQFNRAVDAYRQPGSSFKPYVYTTALLNGFKPTSIVVDGPVCIGNWCPQNYGHSYSGSVTLTQAITRSIN
VVPVKLSITLGGKAGPKAGRAKIVEVARRFGIKAPLIDTPSLPIGSTEVTVLEHAVAYATFPNKGKAPTPHAVLEVRTGA
GDLVWRFDRDGPKQKQAIPASVAADMAGMMSHVVSEGTARRAALDGIPTAGKTGTTNAYRDAWFVGYTGNFSCAVWYGND
DYSPTNRMTGGSLPAQTWRDIMIAAHQGVEIKELAGVGMGTRLPASQAQANARAADDDGKPAPPPVLTRRGADILVRVEK
MLDDAAKAMDKAMDKTPPAKPVSSTGVGFPDSFAAATPNGPPATAPRKN

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=537, Percent_Identity=33.5195530726257, Blast_Score=229, Evalue=6e-61,
Organism=Escherichia coli, GI87082258, Length=301, Percent_Identity=37.8737541528239, Blast_Score=178, Evalue=9e-46,
Organism=Escherichia coli, GI1788867, Length=513, Percent_Identity=29.4346978557505, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1789601, Length=186, Percent_Identity=35.4838709677419, Blast_Score=107, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 83670; Mature: 83670

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAPCPAPSRDWSKPVPKILPDNWMQRIRNWFLDLDARLDSTLFSSAAGIRELWERFSTF
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MDRFYVGHWKRWVFIEPLSEAATMGAGGLLVLLALAQPAFRETGDEDWLKKSDLAVSFLD
HHHHHHHCCCEEEEECCHHHHHHCCCCHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHH
RYGNPIGNRGIKHNDSIPLEDFPDNLIKATLATEDRRFYDHFGIDVAGTARALVTNAQAG
HHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHCCCCCC
GVRQGGSSITQQLAKNLFLSNERTLERKVNEAFLAIWLETRLTKNEILKLYLDRAYMGGG
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC
TFGADGAAHFYFNKSVRDVNLAEAAMLAGLFKAPTKFAPHINLPAARARANVVLDNLVDA
CCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCC
GFMTEGQVFGARRNPASVVDRRDENSPNYYLDWAFEEVRKIVETFPKSYTERVFVVRTTI
CCCCCCCEECCCCCCHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCEEEEEEEEE
DMNVQRAAEAAVENQLRQFGRDYHATQAAAVLSDLDGGVRAMVGGRDYGSSQFNRAVDAY
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHH
RQPGSSFKPYVYTTALLNGFKPTSIVVDGPVCIGNWCPQNYGHSYSGSVTLTQAITRSIN
HCCCCCCCCCEEEHHHHCCCCCCEEEEECCEEECCCCCCCCCCCCCCCEEHHHHHHCCCE
VVPVKLSITLGGKAGPKAGRAKIVEVARRFGIKAPLIDTPSLPIGSTEVTVLEHAVAYAT
EEEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHEE
FPNKGKAPTPHAVLEVRTGAGDLVWRFDRDGPKQKQAIPASVAADMAGMMSHVVSEGTAR
CCCCCCCCCCCEEEEEECCCCCEEEEECCCCCHHHHCCCHHHHHHHHHHHHHHHHCCHHH
RAALDGIPTAGKTGTTNAYRDAWFVGYTGNFSCAVWYGNDDYSPTNRMTGGSLPAQTWRD
HHHHCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHH
IMIAAHQGVEIKELAGVGMGTRLPASQAQANARAADDDGKPAPPPVLTRRGADILVRVEK
HHHHHCCCCCHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHH
MLDDAAKAMDKAMDKTPPAKPVSSTGVGFPDSFAAATPNGPPATAPRKN
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MEAPCPAPSRDWSKPVPKILPDNWMQRIRNWFLDLDARLDSTLFSSAAGIRELWERFSTF
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MDRFYVGHWKRWVFIEPLSEAATMGAGGLLVLLALAQPAFRETGDEDWLKKSDLAVSFLD
HHHHHHHCCCEEEEECCHHHHHHCCCCHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHH
RYGNPIGNRGIKHNDSIPLEDFPDNLIKATLATEDRRFYDHFGIDVAGTARALVTNAQAG
HHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHCCCCCC
GVRQGGSSITQQLAKNLFLSNERTLERKVNEAFLAIWLETRLTKNEILKLYLDRAYMGGG
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC
TFGADGAAHFYFNKSVRDVNLAEAAMLAGLFKAPTKFAPHINLPAARARANVVLDNLVDA
CCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCC
GFMTEGQVFGARRNPASVVDRRDENSPNYYLDWAFEEVRKIVETFPKSYTERVFVVRTTI
CCCCCCCEECCCCCCHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCEEEEEEEEE
DMNVQRAAEAAVENQLRQFGRDYHATQAAAVLSDLDGGVRAMVGGRDYGSSQFNRAVDAY
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHH
RQPGSSFKPYVYTTALLNGFKPTSIVVDGPVCIGNWCPQNYGHSYSGSVTLTQAITRSIN
HCCCCCCCCCEEEHHHHCCCCCCEEEEECCEEECCCCCCCCCCCCCCCEEHHHHHHCCCE
VVPVKLSITLGGKAGPKAGRAKIVEVARRFGIKAPLIDTPSLPIGSTEVTVLEHAVAYAT
EEEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHEE
FPNKGKAPTPHAVLEVRTGAGDLVWRFDRDGPKQKQAIPASVAADMAGMMSHVVSEGTAR
CCCCCCCCCCCEEEEEECCCCCEEEEECCCCCHHHHCCCHHHHHHHHHHHHHHHHCCHHH
RAALDGIPTAGKTGTTNAYRDAWFVGYTGNFSCAVWYGNDDYSPTNRMTGGSLPAQTWRD
HHHHCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHH
IMIAAHQGVEIKELAGVGMGTRLPASQAQANARAADDDGKPAPPPVLTRRGADILVRVEK
HHHHHCCCCCHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHH
MLDDAAKAMDKAMDKTPPAKPVSSTGVGFPDSFAAATPNGPPATAPRKN
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]