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The map label for this gene is phoQ [H]

Identifier: 86750564

GI number: 86750564

Start: 3959508

End: 3960902

Strand: Reverse

Name: phoQ [H]

Synonym: RPB_3453

Alternate gene names: 86750564

Gene position: 3960902-3959508 (Counterclockwise)

Preceding gene: 86750565

Following gene: 86750563

Centisome position: 74.29

GC content: 69.82

Gene sequence:

>1395_bases
ATGCCGAGCCCGGCCGTCCCCGCGCCGCCGCGCCGCCGCACCACCTCGCTGGCCACCCGGCTGTTTCTGTCCGCCACCGC
CTGGGTGGTGGTGATCCTGGCGATCACCGGCATCGTGCTGTCCTCGGTGTACCGCCAGGCCTCCGAGCGCGCGTTCGACC
GGCGGCTCAATCTCTATCTGCGCACCATCATCGCCGAAGTCGCGACGCCCGACGCCGCACCGGAACAGTTCCAGTCGATC
GGCGAGCCGCTGTTCGATCTGCCGCTGTCCGGCTGGTACTGGCAGATCGTCCGCACCGACACCGACAAGGTCGAGCCGCG
CGCCTCGCGCTCGCTGTGGGACCGCAAGCTGCCGAGGCTCGCGGATCAGGGCGTCGAACTCGGCGCCTCCGGCGTTCGCC
AGGGCTATGTCGAGGGGCCGGAGGGCCAGACGCTGCGCATGGTCGAGCGCCCGGTCGATCTCGGCGCCGACGGCAAATTC
GTCGTCACGGTGGCCGGCGACGCCACCGAAATCTTCGAGGAAACAAGGACCTTCGACTACTATCTCGCAGGTACCTTCAT
CGCGCTGTCGATCGGGCTGGTGCTCACCACGATCTTCCAGGTGCGGTTCGGCCTGGCGCCGCTGAACCGCATCTCGCATT
CGATCGCCGACATCCGCTCCGGTCGCGCCGAGCGGCTGGAGGGCCAATTTCCGGTCGAGATCGCGCCGCTGGCGCGCGAA
ACCAACGCGCTGATCGAGGCCAATCGCGAGATCGTCGAACGCGCCCGCACCCATGTCGGCAACCTCGCCCATGCGATCAA
GACGCCGCTGTCGGTGCTGGTCAATGAGGCCGCGGCGCGCCCGGGCGATCCGCTCGCCGCCAAGGTGATGGAGCAGGCCG
AGATCATGCGCAACCAGGTCGCGCATCATCTGGAGCGGGCGCGGATCGCCGCGCGGCTCACCGTGGTCGGCACCGTCACC
GAGGTCGAACCGGTGATCGAGGCGCTGCGCCGCACGATGGAGAAGATCCACCGCGACCGGGCGATTCAGCTCCGCACCGA
AATCGCCGGCGGCCTGAAGTTCCGCGGCGAGAGGCAGGACCTCGAGGAGATGGTCGGCAACCTGGTCGACAATGCCTGCA
AATGGGCGGTCGGCCGGGTGCTGATCGCGGCCACCGCCGAACGCGGCCCGACGCCGCTGCTGCGGATCATCGTCGACGAC
GACGGCCGCGGCCTGTCGGCGGCGGAGCAGGCGCAGGTCGCCCGCCGCGGCCAGCGGCTCGACGAGAGCAAGCCGGGCTC
GGGTCTGGGGCTGGCGATTGTCGTCGACCTGGCAGCGCTCTACGGCGGCGAATTGATTCTCGGCCACGCCCCGGCCGGCG
GCCTGCGCGCGGAATTGCTGCTGCCTGCGGCATAA

Upstream 100 bases:

>100_bases
CGCGGCCTCGGCTATCTGCTGACGCCGCCGCCGGCCGACGCCCAGCTGGCGAACCGCACGTGAGCGAGCGGGCGAGGGCG
TCGCTGCAGAAGCCGGGCTG

Downstream 100 bases:

>100_bases
CGAGCCGGCCGCTGCCACAGCGTCGCCCGAATTTGTCGCTCCGTTGCCTTGAGCGTTAGGGATTTCGGGGTAAGACGTTG
GTGGGGAATGGATTGGGGAC

Product: periplasmic sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MPSPAVPAPPRRRTTSLATRLFLSATAWVVVILAITGIVLSSVYRQASERAFDRRLNLYLRTIIAEVATPDAAPEQFQSI
GEPLFDLPLSGWYWQIVRTDTDKVEPRASRSLWDRKLPRLADQGVELGASGVRQGYVEGPEGQTLRMVERPVDLGADGKF
VVTVAGDATEIFEETRTFDYYLAGTFIALSIGLVLTTIFQVRFGLAPLNRISHSIADIRSGRAERLEGQFPVEIAPLARE
TNALIEANREIVERARTHVGNLAHAIKTPLSVLVNEAAARPGDPLAAKVMEQAEIMRNQVAHHLERARIAARLTVVGTVT
EVEPVIEALRRTMEKIHRDRAIQLRTEIAGGLKFRGERQDLEEMVGNLVDNACKWAVGRVLIAATAERGPTPLLRIIVDD
DGRGLSAAEQAQVARRGQRLDESKPGSGLGLAIVVDLAALYGGELILGHAPAGGLRAELLLPAA

Sequences:

>Translated_464_residues
MPSPAVPAPPRRRTTSLATRLFLSATAWVVVILAITGIVLSSVYRQASERAFDRRLNLYLRTIIAEVATPDAAPEQFQSI
GEPLFDLPLSGWYWQIVRTDTDKVEPRASRSLWDRKLPRLADQGVELGASGVRQGYVEGPEGQTLRMVERPVDLGADGKF
VVTVAGDATEIFEETRTFDYYLAGTFIALSIGLVLTTIFQVRFGLAPLNRISHSIADIRSGRAERLEGQFPVEIAPLARE
TNALIEANREIVERARTHVGNLAHAIKTPLSVLVNEAAARPGDPLAAKVMEQAEIMRNQVAHHLERARIAARLTVVGTVT
EVEPVIEALRRTMEKIHRDRAIQLRTEIAGGLKFRGERQDLEEMVGNLVDNACKWAVGRVLIAATAERGPTPLLRIIVDD
DGRGLSAAEQAQVARRGQRLDESKPGSGLGLAIVVDLAALYGGELILGHAPAGGLRAELLLPAA
>Mature_463_residues
PSPAVPAPPRRRTTSLATRLFLSATAWVVVILAITGIVLSSVYRQASERAFDRRLNLYLRTIIAEVATPDAAPEQFQSIG
EPLFDLPLSGWYWQIVRTDTDKVEPRASRSLWDRKLPRLADQGVELGASGVRQGYVEGPEGQTLRMVERPVDLGADGKFV
VTVAGDATEIFEETRTFDYYLAGTFIALSIGLVLTTIFQVRFGLAPLNRISHSIADIRSGRAERLEGQFPVEIAPLARET
NALIEANREIVERARTHVGNLAHAIKTPLSVLVNEAAARPGDPLAAKVMEQAEIMRNQVAHHLERARIAARLTVVGTVTE
VEPVIEALRRTMEKIHRDRAIQLRTEIAGGLKFRGERQDLEEMVGNLVDNACKWAVGRVLIAATAERGPTPLLRIIVDDD
GRGLSAAEQAQVARRGQRLDESKPGSGLGLAIVVDLAALYGGELILGHAPAGGLRAELLLPAA

Specific function: Member of the two-component regulatory system phoQ/phoP involved in virulence, adaptation to low Mg(2+) environments and the control of acid resistance genes. In presence of low periplasmic Mg(2+) concentrations, phoQ functions as a membrane- associated p

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787374, Length=264, Percent_Identity=27.6515151515151, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1786783, Length=345, Percent_Identity=23.1884057971014, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1788393, Length=274, Percent_Identity=24.0875912408759, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1789403, Length=358, Percent_Identity=25.9776536312849, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR015014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF08918 PhoQ_Sensor [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50565; Mature: 50434

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSPAVPAPPRRRTTSLATRLFLSATAWVVVILAITGIVLSSVYRQASERAFDRRLNLYL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTIIAEVATPDAAPEQFQSIGEPLFDLPLSGWYWQIVRTDTDKVEPRASRSLWDRKLPRL
HHHHHHHCCCCCCHHHHHHHCCHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHH
ADQGVELGASGVRQGYVEGPEGQTLRMVERPVDLGADGKFVVTVAGDATEIFEETRTFDY
HHCCHHCCCHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHH
YLAGTFIALSIGLVLTTIFQVRFGLAPLNRISHSIADIRSGRAERLEGQFPVEIAPLARE
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHCCCCCCEEEECHHHH
TNALIEANREIVERARTHVGNLAHAIKTPLSVLVNEAAARPGDPLAAKVMEQAEIMRNQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
AHHLERARIAARLTVVGTVTEVEPVIEALRRTMEKIHRDRAIQLRTEIAGGLKFRGERQD
HHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
LEEMVGNLVDNACKWAVGRVLIAATAERGPTPLLRIIVDDDGRGLSAAEQAQVARRGQRL
HHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHC
DESKPGSGLGLAIVVDLAALYGGELILGHAPAGGLRAELLLPAA
CCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCEECEECCCC
>Mature Secondary Structure 
PSPAVPAPPRRRTTSLATRLFLSATAWVVVILAITGIVLSSVYRQASERAFDRRLNLYL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTIIAEVATPDAAPEQFQSIGEPLFDLPLSGWYWQIVRTDTDKVEPRASRSLWDRKLPRL
HHHHHHHCCCCCCHHHHHHHCCHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHH
ADQGVELGASGVRQGYVEGPEGQTLRMVERPVDLGADGKFVVTVAGDATEIFEETRTFDY
HHCCHHCCCHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHH
YLAGTFIALSIGLVLTTIFQVRFGLAPLNRISHSIADIRSGRAERLEGQFPVEIAPLARE
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHCCCCCCEEEECHHHH
TNALIEANREIVERARTHVGNLAHAIKTPLSVLVNEAAARPGDPLAAKVMEQAEIMRNQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
AHHLERARIAARLTVVGTVTEVEPVIEALRRTMEKIHRDRAIQLRTEIAGGLKFRGERQD
HHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
LEEMVGNLVDNACKWAVGRVLIAATAERGPTPLLRIIVDDDGRGLSAAEQAQVARRGQRL
HHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHC
DESKPGSGLGLAIVVDLAALYGGELILGHAPAGGLRAELLLPAA
CCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCEECEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]