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The map label for this gene is irlS [H]

Identifier: 86750552

GI number: 86750552

Start: 3946246

End: 3947715

Strand: Reverse

Name: irlS [H]

Synonym: RPB_3441

Alternate gene names: 86750552

Gene position: 3947715-3946246 (Counterclockwise)

Preceding gene: 86750553

Following gene: 86750551

Centisome position: 74.04

GC content: 68.84

Gene sequence:

>1470_bases
GTGACGGCATTCGGTAAACTGATCCGAACCACCGCGTTTCGGCTGACGCTGGTTTATCTGTTCCTGTTCGGGCTGTTCGC
GGCGTCGCTGCTGGGGTATTTCGCCTGGAACACGCGGCGGCTGATCACCGATCAGATCACCACCACGGTGGATGCCGAGA
TCGGCGAGATCGACGAGGTCTATACGCGGCGGGGCCTGCGCGGGCTGGTGCTGGCGATCGAGAACCGGGCGCTGCGGCCG
GGCGCCAATCTCTATCTCGTCACCACGCCGGCGGGGCAGGCGGTCGCCGGCAATGTCGGCGCGCTGGCGCCCGGCGTGAT
GAGCAAGATCGGCTGGACCGAAACCTTCTATCGAAGGCTCGACGACCAGGACCGCGGCGAGCATCGCGCGCTGGTCCGCG
TCACCGAACTGTCGAGCGGCTTCCGGCTGCTGATCGGCCGCGACCTCGAGGAACGCCGGCGGCTGTTCAACGTGGTGCGC
TCGGCGGCGCAATGGTCGGTGCTGATCGTCGTCGTGCTCGGGCTCGGCGGCGGCATCTTCGTCGCGCGCCGCGTGTTGCG
CCGGATCGACGCGATGACCGGCACGACGCAGCGGATCATGGCCGGCGACCTGTCCGGCCGGCTGCCGGTCGGCCGCAGCG
GCGACGAACTCGATCGGCTGGCGGAAAATCTCAACGCCATGCTGGAGCGGATCGAGGCGCTGATGAACGGCCTCAAGGAG
GTCTCCGACAATATCGCGCACGATCTGAAGACGCCGCTGACGCGGCTGCGCAACCGCGCCGAGGAGGCGCTGGCGCTGTC
CGGCAGCGAGGCCGATTATCGCGCCGCACTGGAGCGCACCATCGAGGAATCCGACGGCCTGATCCGCACCTTCAACGCGC
TCCTGATGATCGCGCGCGCGGAATCGGGACATGCGCGCGGACAAATGGACGCGTTCGACGCGGCCGGAATCGTGCGCGAC
ATTCACGAATTGTACGAGCCGCTGGCGGAAGACAACGGCCTGACGCTGCAGGTCCGGAGCGCGCCGGCGCCGGTGCGCGG
CAATCGCGAATTGATCAGCCAGGCGCTCGCCAATCTGGTCGAGAACGCGATCAAATACGGCCAGCCGCAGCTCGCGCTGG
CGCCGCCCGGCGACGCGCCGGCCACGGCCCCGCTGCCGGGCGCGGAGATCGTGATCGAGGCGCAGCGGGACGGCGACCAG
GTGCTGCTCAGCGTGACCGATCGCGGTCGCGGAATACCGGAAGCGGACCGCAAGCACGCGGTCGAACGCTTCGTGCGGCT
GGAGGCGAGCCGCACCTTGCCGGGTTCGGGGCTCGGCCTCAGCCTGGCCTCCGCGGTGGCGACGCTGCATGGCGGCGAAC
TGCGGCTGTCCGACGCGCAACCGGGTTTGCGCGCCACGCTTGCAATTCCGGCGCACACGGGCGAAGGACTGGCACCAGCC
AACACCATACCAGAACCGACGCCCGCATGA

Upstream 100 bases:

>100_bases
AACGTCATCGACGTCCACATCTCCCGCCTGCGCGGCAAGATCGACAAGGGCTTCGACCGTCCGCTGCTGCACACCATCCG
CGGCGCGGGGTATATGATCC

Downstream 100 bases:

>100_bases
GTTCCTCCGCGATTGACGCGGACATTTCACCCGCCTCACTAGCCGCGCGTTTCGCCGCCGGGCCGCAATTGCATGCGCCC
GACGAGGCGGAAAAGCGCTG

Product: periplasmic sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MTAFGKLIRTTAFRLTLVYLFLFGLFAASLLGYFAWNTRRLITDQITTTVDAEIGEIDEVYTRRGLRGLVLAIENRALRP
GANLYLVTTPAGQAVAGNVGALAPGVMSKIGWTETFYRRLDDQDRGEHRALVRVTELSSGFRLLIGRDLEERRRLFNVVR
SAAQWSVLIVVVLGLGGGIFVARRVLRRIDAMTGTTQRIMAGDLSGRLPVGRSGDELDRLAENLNAMLERIEALMNGLKE
VSDNIAHDLKTPLTRLRNRAEEALALSGSEADYRAALERTIEESDGLIRTFNALLMIARAESGHARGQMDAFDAAGIVRD
IHELYEPLAEDNGLTLQVRSAPAPVRGNRELISQALANLVENAIKYGQPQLALAPPGDAPATAPLPGAEIVIEAQRDGDQ
VLLSVTDRGRGIPEADRKHAVERFVRLEASRTLPGSGLGLSLASAVATLHGGELRLSDAQPGLRATLAIPAHTGEGLAPA
NTIPEPTPA

Sequences:

>Translated_489_residues
MTAFGKLIRTTAFRLTLVYLFLFGLFAASLLGYFAWNTRRLITDQITTTVDAEIGEIDEVYTRRGLRGLVLAIENRALRP
GANLYLVTTPAGQAVAGNVGALAPGVMSKIGWTETFYRRLDDQDRGEHRALVRVTELSSGFRLLIGRDLEERRRLFNVVR
SAAQWSVLIVVVLGLGGGIFVARRVLRRIDAMTGTTQRIMAGDLSGRLPVGRSGDELDRLAENLNAMLERIEALMNGLKE
VSDNIAHDLKTPLTRLRNRAEEALALSGSEADYRAALERTIEESDGLIRTFNALLMIARAESGHARGQMDAFDAAGIVRD
IHELYEPLAEDNGLTLQVRSAPAPVRGNRELISQALANLVENAIKYGQPQLALAPPGDAPATAPLPGAEIVIEAQRDGDQ
VLLSVTDRGRGIPEADRKHAVERFVRLEASRTLPGSGLGLSLASAVATLHGGELRLSDAQPGLRATLAIPAHTGEGLAPA
NTIPEPTPA
>Mature_488_residues
TAFGKLIRTTAFRLTLVYLFLFGLFAASLLGYFAWNTRRLITDQITTTVDAEIGEIDEVYTRRGLRGLVLAIENRALRPG
ANLYLVTTPAGQAVAGNVGALAPGVMSKIGWTETFYRRLDDQDRGEHRALVRVTELSSGFRLLIGRDLEERRRLFNVVRS
AAQWSVLIVVVLGLGGGIFVARRVLRRIDAMTGTTQRIMAGDLSGRLPVGRSGDELDRLAENLNAMLERIEALMNGLKEV
SDNIAHDLKTPLTRLRNRAEEALALSGSEADYRAALERTIEESDGLIRTFNALLMIARAESGHARGQMDAFDAAGIVRDI
HELYEPLAEDNGLTLQVRSAPAPVRGNRELISQALANLVENAIKYGQPQLALAPPGDAPATAPLPGAEIVIEAQRDGDQV
LLSVTDRGRGIPEADRKHAVERFVRLEASRTLPGSGLGLSLASAVATLHGGELRLSDAQPGLRATLAIPAHTGEGLAPAN
TIPEPTPA

Specific function: Member of the two-component regulatory system irlR/irlS. May be involved in invasion of eukaryotic cells and heavy-metal resistance. Probably activates irlR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=339, Percent_Identity=28.023598820059, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1788279, Length=364, Percent_Identity=24.4505494505494, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1787894, Length=300, Percent_Identity=25.6666666666667, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1789808, Length=254, Percent_Identity=25.5905511811024, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1790861, Length=250, Percent_Identity=29.2, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI1786912, Length=215, Percent_Identity=29.7674418604651, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1790346, Length=255, Percent_Identity=25.0980392156863, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1790551, Length=317, Percent_Identity=25.2365930599369, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1788393, Length=277, Percent_Identity=24.5487364620939, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 52842; Mature: 52711

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAFGKLIRTTAFRLTLVYLFLFGLFAASLLGYFAWNTRRLITDQITTTVDAEIGEIDEV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHH
YTRRGLRGLVLAIENRALRPGANLYLVTTPAGQAVAGNVGALAPGVMSKIGWTETFYRRL
HHHCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCHHCCHHHHHHHCHHHHHHHHC
DDQDRGEHRALVRVTELSSGFRLLIGRDLEERRRLFNVVRSAAQWSVLIVVVLGLGGGIF
CCCCCCCHHHEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
VARRVLRRIDAMTGTTQRIMAGDLSGRLPVGRSGDELDRLAENLNAMLERIEALMNGLKE
HHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VSDNIAHDLKTPLTRLRNRAEEALALSGSEADYRAALERTIEESDGLIRTFNALLMIARA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHEEC
ESGHARGQMDAFDAAGIVRDIHELYEPLAEDNGLTLQVRSAPAPVRGNRELISQALANLV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHH
ENAIKYGQPQLALAPPGDAPATAPLPGAEIVIEAQRDGDQVLLSVTDRGRGIPEADRKHA
HHHHHCCCCCEEECCCCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCHHHHHH
VERFVRLEASRTLPGSGLGLSLASAVATLHGGELRLSDAQPGLRATLAIPAHTGEGLAPA
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCEEEEEEECCCCCCCCCC
NTIPEPTPA
CCCCCCCCC
>Mature Secondary Structure 
TAFGKLIRTTAFRLTLVYLFLFGLFAASLLGYFAWNTRRLITDQITTTVDAEIGEIDEV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHH
YTRRGLRGLVLAIENRALRPGANLYLVTTPAGQAVAGNVGALAPGVMSKIGWTETFYRRL
HHHCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCHHCCHHHHHHHCHHHHHHHHC
DDQDRGEHRALVRVTELSSGFRLLIGRDLEERRRLFNVVRSAAQWSVLIVVVLGLGGGIF
CCCCCCCHHHEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
VARRVLRRIDAMTGTTQRIMAGDLSGRLPVGRSGDELDRLAENLNAMLERIEALMNGLKE
HHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VSDNIAHDLKTPLTRLRNRAEEALALSGSEADYRAALERTIEESDGLIRTFNALLMIARA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHEEC
ESGHARGQMDAFDAAGIVRDIHELYEPLAEDNGLTLQVRSAPAPVRGNRELISQALANLV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHH
ENAIKYGQPQLALAPPGDAPATAPLPGAEIVIEAQRDGDQVLLSVTDRGRGIPEADRKHA
HHHHHCCCCCEEECCCCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCHHHHHH
VERFVRLEASRTLPGSGLGLSLASAVATLHGGELRLSDAQPGLRATLAIPAHTGEGLAPA
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCEEEEEEECCCCCCCCCC
NTIPEPTPA
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9393784 [H]