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The map label for this gene is nadE [H]

Identifier: 86750492

GI number: 86750492

Start: 3864911

End: 3866668

Strand: Reverse

Name: nadE [H]

Synonym: RPB_3381

Alternate gene names: 86750492

Gene position: 3866668-3864911 (Counterclockwise)

Preceding gene: 86750493

Following gene: 86750491

Centisome position: 72.52

GC content: 68.09

Gene sequence:

>1758_bases
ATGACCAACACAGCCGCCCGTTTCACGATCTCGCTCGCCCAGCTCAATCCGACGGTCGGCGACATCACCGGCAACGCCGC
GATGGCGCGCGCGGCGCGTGCGCAGGCCGCGGCCGAGAACGCCGATGTCATCGTCTTTCCCGAGCTGTTCATCTCCGGCT
ATCCGCCGGAAGATCTGGTGCTGAAGCCGGCGTTTCAGGCCGCGTGCCGCGCGGCGGTCGAGGAACTGGCGCGCGAGACC
GCCGATGGCGGGCCGGCAATGCTGATCGGCTCGCCCTGGGTCGACGGCGGCAAGCTCTACAACGCCTGCGCATTGCTCGA
CGGCGGCCGCATCGCGGCGATCCGCTTCAAGGTCAATCTGCCGAACTACGGCGTGTTCGACGAGAAGCGCGTGTTCGCGC
GCGGGCCGGTGGCGGGTCCGGTGACGATCCGCGGCGTCCGCGTCGGCGTGCCGATCTGCGAGGACACCTGGCTCGAGGAG
TCCGAGGACTACGAGAACGTCGTCGAGTGCTTGGCCGAGACCGGCGCCGAAATCCTGATCGTGCCGAACGGCTCACCCTA
CGCGCGCGGCAAGGGCGATCTGCGGCTGTCGGTGCAGGTCGCCCGCGTCACCGAGAGCGATCTGCCGCTGGTCTATCTCA
ATCAGGTCGGCGGCCAGGACGAACTGGTGTTCGACGGCGCATCCTTTGCGCTCAACGCCGACCGCTCGCTCGCCGCGCAA
CTCCCCGCCTTCGCCGAATGCATCACCACGCTGGTCTGGCAGAAGAGCGCGGATGGCTGGCGCTGCGATGGCCCGGTGGC
GGCGGTGCCCGACGGCGACGAGGGCGACTACGCCGCCTGCGTGCTGGGGCTACGCGACTACGTCCGCAAGAACGGTTTCC
CCGGTGTGCTGCTCGGCATCTCCGGCGGCATCGATTCCGCGCTGTGCGCCGCGATCGCGGTCGATGCGCTCGGCGCGGGC
AGGGTCCGCGGCGTGATGCTGCCGTTCCGCTACACCGCGCAGATCTCGCTCGACGATGCCAGTAGGCTCGCGAATGCGCT
GGGCTTCGGCTACGAGGTGCTGCCGATCGCTCAGGCGGTGGAAGGCTTCGAGGCGATCCTGGCGAAGCCGTTCGCCGGCC
TCGCGCGCGACATCACCGAAGAAAACCTGCAGGCGCGCACCCGCGGCACGCTGCTGATGGCGATCTCCAACAAGACCGGC
GCGATGGTGGTCACCACGGGTAACAAGTCGGAAATGAGCGTCGGCTACGCCACGCTGTATGGCGACATGAACGGCGGCTT
CAATCCGATCAAGGACATCTACAAGACGCAAGTGTTCCGCCTCTCCAGCCTGCGCAATCGCTGGAAGCCCGACGGCGCGC
TCGGCCCCGACGGTGAGGTGATCCCGGAAAGCATCATCGTCCGTCCGCCGACCGCGGAACTGCGTGAAAACCAGACCGAC
CAGGATTCGCTGCCGCCCTACGAGGTGCTCGACGCCATCCTCGAACGCCTGGTCGAACGCGAGCAGCCGCTGGCGTCGAT
CGTCGCCGAGGGCTTCGACAAGGACACCGTGATGCGGATCGACCGCCTCCTCAACATCGCCGAATACAAGCGCCGCCAGG
CCGCCCCCGGCGTCAAGGTGACCCGCAAGAACTTCGGCCGCGACCGCCGCTACCCGATCACCAACCGTTTTCGCGACAAG
GCCGAGGCGCTGGCCGAGCCGGACGAGGCGCTGGTGATGCGGGCAGGGCGTGCGTCGAGCGAGGCGTTCGAGGGGTAG

Upstream 100 bases:

>100_bases
CCATTGCCGGGATGGTCTGGCTGTTCGCGCTCGGCGAGCGCATGGGGCTGTTCTAGTTCCCCCGATTGCGAAGCGGGCAG
GGGGCGGCTAAATCTCGGTG

Downstream 100 bases:

>100_bases
GAGGAAGTCATGGGCGCGGACTGCCAATCTTTCGCAGATGCCGGGCGCTCCGTTTGTTCGACTTGCAAAGGACCTGATGA
GTGTGGCGATAGCCGATCCG

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 585; Mature: 584

Protein sequence:

>585_residues
MTNTAARFTISLAQLNPTVGDITGNAAMARAARAQAAAENADVIVFPELFISGYPPEDLVLKPAFQAACRAAVEELARET
ADGGPAMLIGSPWVDGGKLYNACALLDGGRIAAIRFKVNLPNYGVFDEKRVFARGPVAGPVTIRGVRVGVPICEDTWLEE
SEDYENVVECLAETGAEILIVPNGSPYARGKGDLRLSVQVARVTESDLPLVYLNQVGGQDELVFDGASFALNADRSLAAQ
LPAFAECITTLVWQKSADGWRCDGPVAAVPDGDEGDYAACVLGLRDYVRKNGFPGVLLGISGGIDSALCAAIAVDALGAG
RVRGVMLPFRYTAQISLDDASRLANALGFGYEVLPIAQAVEGFEAILAKPFAGLARDITEENLQARTRGTLLMAISNKTG
AMVVTTGNKSEMSVGYATLYGDMNGGFNPIKDIYKTQVFRLSSLRNRWKPDGALGPDGEVIPESIIVRPPTAELRENQTD
QDSLPPYEVLDAILERLVEREQPLASIVAEGFDKDTVMRIDRLLNIAEYKRRQAAPGVKVTRKNFGRDRRYPITNRFRDK
AEALAEPDEALVMRAGRASSEAFEG

Sequences:

>Translated_585_residues
MTNTAARFTISLAQLNPTVGDITGNAAMARAARAQAAAENADVIVFPELFISGYPPEDLVLKPAFQAACRAAVEELARET
ADGGPAMLIGSPWVDGGKLYNACALLDGGRIAAIRFKVNLPNYGVFDEKRVFARGPVAGPVTIRGVRVGVPICEDTWLEE
SEDYENVVECLAETGAEILIVPNGSPYARGKGDLRLSVQVARVTESDLPLVYLNQVGGQDELVFDGASFALNADRSLAAQ
LPAFAECITTLVWQKSADGWRCDGPVAAVPDGDEGDYAACVLGLRDYVRKNGFPGVLLGISGGIDSALCAAIAVDALGAG
RVRGVMLPFRYTAQISLDDASRLANALGFGYEVLPIAQAVEGFEAILAKPFAGLARDITEENLQARTRGTLLMAISNKTG
AMVVTTGNKSEMSVGYATLYGDMNGGFNPIKDIYKTQVFRLSSLRNRWKPDGALGPDGEVIPESIIVRPPTAELRENQTD
QDSLPPYEVLDAILERLVEREQPLASIVAEGFDKDTVMRIDRLLNIAEYKRRQAAPGVKVTRKNFGRDRRYPITNRFRDK
AEALAEPDEALVMRAGRASSEAFEG
>Mature_584_residues
TNTAARFTISLAQLNPTVGDITGNAAMARAARAQAAAENADVIVFPELFISGYPPEDLVLKPAFQAACRAAVEELARETA
DGGPAMLIGSPWVDGGKLYNACALLDGGRIAAIRFKVNLPNYGVFDEKRVFARGPVAGPVTIRGVRVGVPICEDTWLEES
EDYENVVECLAETGAEILIVPNGSPYARGKGDLRLSVQVARVTESDLPLVYLNQVGGQDELVFDGASFALNADRSLAAQL
PAFAECITTLVWQKSADGWRCDGPVAAVPDGDEGDYAACVLGLRDYVRKNGFPGVLLGISGGIDSALCAAIAVDALGAGR
VRGVMLPFRYTAQISLDDASRLANALGFGYEVLPIAQAVEGFEAILAKPFAGLARDITEENLQARTRGTLLMAISNKTGA
MVVTTGNKSEMSVGYATLYGDMNGGFNPIKDIYKTQVFRLSSLRNRWKPDGALGPDGEVIPESIIVRPPTAELRENQTDQ
DSLPPYEVLDAILERLVEREQPLASIVAEGFDKDTVMRIDRLLNIAEYKRRQAAPGVKVTRKNFGRDRRYPITNRFRDKA
EALAEPDEALVMRAGRASSEAFEG

Specific function: It is not known if this NAD synthase uses NH(3) or glutamine as the N donor [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 62893; Mature: 62762

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNTAARFTISLAQLNPTVGDITGNAAMARAARAQAAAENADVIVFPELFISGYPPEDLV
CCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEHHHHHCCCCCCCCE
LKPAFQAACRAAVEELARETADGGPAMLIGSPWVDGGKLYNACALLDGGRIAAIRFKVNL
ECHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHEEEEECCCEEEEEEEEEEC
PNYGVFDEKRVFARGPVAGPVTIRGVRVGVPICEDTWLEESEDYENVVECLAETGAEILI
CCCCCCCCCCEEECCCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEE
VPNGSPYARGKGDLRLSVQVARVTESDLPLVYLNQVGGQDELVFDGASFALNADRSLAAQ
EECCCCCCCCCCCEEEEEEEEEECCCCCCEEEEECCCCCCCEEECCCCEEECCCHHHHHH
LPAFAECITTLVWQKSADGWRCDGPVAAVPDGDEGDYAACVLGLRDYVRKNGFPGVLLGI
CHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEE
SGGIDSALCAAIAVDALGAGRVRGVMLPFRYTAQISLDDASRLANALGFGYEVLPIAQAV
CCCCHHHHHHHHHHHHCCCCCEEEEEECEEEEEEEECCHHHHHHHHHCCCCHHHHHHHHH
EGFEAILAKPFAGLARDITEENLQARTRGTLLMAISNKTGAMVVTTGNKSEMSVGYATLY
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCCCCCEEEEEEEE
GDMNGGFNPIKDIYKTQVFRLSSLRNRWKPDGALGPDGEVIPESIIVRPPTAELRENQTD
ECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCHHHHCCCCC
QDSLPPYEVLDAILERLVEREQPLASIVAEGFDKDTVMRIDRLLNIAEYKRRQAAPGVKV
CCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEE
TRKNFGRDRRYPITNRFRDKAEALAEPDEALVMRAGRASSEAFEG
HHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHEECCCCCCCCCCC
>Mature Secondary Structure 
TNTAARFTISLAQLNPTVGDITGNAAMARAARAQAAAENADVIVFPELFISGYPPEDLV
CCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEHHHHHCCCCCCCCE
LKPAFQAACRAAVEELARETADGGPAMLIGSPWVDGGKLYNACALLDGGRIAAIRFKVNL
ECHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHEEEEECCCEEEEEEEEEEC
PNYGVFDEKRVFARGPVAGPVTIRGVRVGVPICEDTWLEESEDYENVVECLAETGAEILI
CCCCCCCCCCEEECCCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEE
VPNGSPYARGKGDLRLSVQVARVTESDLPLVYLNQVGGQDELVFDGASFALNADRSLAAQ
EECCCCCCCCCCCEEEEEEEEEECCCCCCEEEEECCCCCCCEEECCCCEEECCCHHHHHH
LPAFAECITTLVWQKSADGWRCDGPVAAVPDGDEGDYAACVLGLRDYVRKNGFPGVLLGI
CHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEE
SGGIDSALCAAIAVDALGAGRVRGVMLPFRYTAQISLDDASRLANALGFGYEVLPIAQAV
CCCCHHHHHHHHHHHHCCCCCEEEEEECEEEEEEEECCHHHHHHHHHCCCCHHHHHHHHH
EGFEAILAKPFAGLARDITEENLQARTRGTLLMAISNKTGAMVVTTGNKSEMSVGYATLY
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCCCCCEEEEEEEE
GDMNGGFNPIKDIYKTQVFRLSSLRNRWKPDGALGPDGEVIPESIIVRPPTAELRENQTD
ECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCHHHHCCCCC
QDSLPPYEVLDAILERLVEREQPLASIVAEGFDKDTVMRIDRLLNIAEYKRRQAAPGVKV
CCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEE
TRKNFGRDRRYPITNRFRDKAEALAEPDEALVMRAGRASSEAFEG
HHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8431308; 8195100 [H]