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The map label for this gene is bepF [H]

Identifier: 86750415

GI number: 86750415

Start: 3778449

End: 3779639

Strand: Reverse

Name: bepF [H]

Synonym: RPB_3304

Alternate gene names: 86750415

Gene position: 3779639-3778449 (Counterclockwise)

Preceding gene: 86750431

Following gene: 86750414

Centisome position: 70.89

GC content: 69.86

Gene sequence:

>1191_bases
ATGGCCAGCAAATCCGCTCGAAACGTCTCGCTGGGGGCCGGCCTTGTGGCGCTCTTCGCCGCCGTCGCCGGTGGCGCGAC
GCTGATCGACCGCTCTGGCGCGGCCGCCCGGGACACCCCGGCGCCGCCCGCCGTGGCTGCTGCTGTGGCCACCGTCGAGA
CCAGGCCGACCATGGCCGCCGATGAGTTTTCGGGGCGGCTCGAAGCCGTCGAGCGGGTCGAGATCCGCTCGCGCGTCGCC
GGCGCGGTGCAGGAGATCCGATTCCGCGAAGGCGCGCTGGTGACCAAGGGCGACCTGCTGGTGGTGATCGATCCGTCGCT
CTACGCCGCCGACGTCGATCGCGCGCAGGCGCAGCTCTCGTCTGCGCGCGCCCGCCTGACGCTGGCAAAGGCCGATTTCG
AGCGGGGGCAGCAGCTGTCGAGCTCGAGCATCACCCAGCGCGAGCTCGACACCCGGGCCAACGCCTATCGCGAGGCGGAG
GCCAGCCTCAGAACCGCCGAGGCGGCGCTGAAGACCGCCGAACTCAACCTCGGCTACACCAATATCCGCGCGCCGGTGTC
CGGCCGGGTCGGCAAGGTGGAGATTACGGTGGGCAATCTGATCGCCGCCGGACCGAGTTCGCCGCTGCTGACCACGCTGG
TGTCGGTCGATCCGATCTATGCCAGCTTCAATGCCGACGAGCAGGTGGTGACGCGTGCGCTGAAGACGCTGGCCGACGAG
CGTGCGCCGACGGCGATCGACCGCATTCCGGTGCAGATGACGACCGGAGCGGGCGGTGCGCCGGTGAAGGGCCGGATGCA
GTTCATCGACAATCAGGTCGATCCGCGCTCCGGCACGATTCGGGTGCGCGCGGTGTTCGACAATGCCGATGGCCGGCTGA
TGCCGGGCCAATTCGCGCGGCTGTCGATGGGCCAGCCGAAGCCGCAGCCGGCGCTGCTGGTCAGCGAGCGCGCGGTCGGC
ACCGACCAGAACAAGAAGTTCGTGATGGTGGTCGATGCCAGGAACCAGGCCGAATATCGCGAAGTCACGCTCGGCGCCTC
GGTGGAGGGGTTGCGCGTCGTCGCCTCCGGCCTGAAGGCCGGCGAGCGCATCGTGGTCAACGGCCTGCAGCGCGTCCGCC
CGGGCGTGACCATCAAGCCGGAGACGGTGGCGATCGACGCCGCATCCGGCAGCGCGGTGGCGCAGAACTAA

Upstream 100 bases:

>100_bases
ATCGCAGCATGGTGTGATGATGCTCCCCGTTGTCGGAAGAGCATCCCAGTTACTGAAATTGCGCGGCTTGGTGCAAGTCG
CAACGATCAAAGAGGTTCAG

Downstream 100 bases:

>100_bases
TCGTCACCCGTCGCGTCCGAGCGCGGCGGGGCGTCTTTCATTCACAGACCGACAGCAGCGCAGCCGCATCCTCGGCCGCG
CAGGGATCTCTGCGCCCCGC

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MASKSARNVSLGAGLVALFAAVAGGATLIDRSGAAARDTPAPPAVAAAVATVETRPTMAADEFSGRLEAVERVEIRSRVA
GAVQEIRFREGALVTKGDLLVVIDPSLYAADVDRAQAQLSSARARLTLAKADFERGQQLSSSSITQRELDTRANAYREAE
ASLRTAEAALKTAELNLGYTNIRAPVSGRVGKVEITVGNLIAAGPSSPLLTTLVSVDPIYASFNADEQVVTRALKTLADE
RAPTAIDRIPVQMTTGAGGAPVKGRMQFIDNQVDPRSGTIRVRAVFDNADGRLMPGQFARLSMGQPKPQPALLVSERAVG
TDQNKKFVMVVDARNQAEYREVTLGASVEGLRVVASGLKAGERIVVNGLQRVRPGVTIKPETVAIDAASGSAVAQN

Sequences:

>Translated_396_residues
MASKSARNVSLGAGLVALFAAVAGGATLIDRSGAAARDTPAPPAVAAAVATVETRPTMAADEFSGRLEAVERVEIRSRVA
GAVQEIRFREGALVTKGDLLVVIDPSLYAADVDRAQAQLSSARARLTLAKADFERGQQLSSSSITQRELDTRANAYREAE
ASLRTAEAALKTAELNLGYTNIRAPVSGRVGKVEITVGNLIAAGPSSPLLTTLVSVDPIYASFNADEQVVTRALKTLADE
RAPTAIDRIPVQMTTGAGGAPVKGRMQFIDNQVDPRSGTIRVRAVFDNADGRLMPGQFARLSMGQPKPQPALLVSERAVG
TDQNKKFVMVVDARNQAEYREVTLGASVEGLRVVASGLKAGERIVVNGLQRVRPGVTIKPETVAIDAASGSAVAQN
>Mature_395_residues
ASKSARNVSLGAGLVALFAAVAGGATLIDRSGAAARDTPAPPAVAAAVATVETRPTMAADEFSGRLEAVERVEIRSRVAG
AVQEIRFREGALVTKGDLLVVIDPSLYAADVDRAQAQLSSARARLTLAKADFERGQQLSSSSITQRELDTRANAYREAEA
SLRTAEAALKTAELNLGYTNIRAPVSGRVGKVEITVGNLIAAGPSSPLLTTLVSVDPIYASFNADEQVVTRALKTLADER
APTAIDRIPVQMTTGAGGAPVKGRMQFIDNQVDPRSGTIRVRAVFDNADGRLMPGQFARLSMGQPKPQPALLVSERAVGT
DQNKKFVMVVDARNQAEYREVTLGASVEGLRVVASGLKAGERIVVNGLQRVRPGVTIKPETVAIDAASGSAVAQN

Specific function: May contribute to resistance to some drugs, such as deoxycholate, sodium dodecyl sulfate and nalidixic acid, in the absence of BepD and BepE [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=358, Percent_Identity=34.0782122905028, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1789929, Length=318, Percent_Identity=32.3899371069182, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1789665, Length=338, Percent_Identity=32.2485207100592, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI87082036, Length=318, Percent_Identity=32.0754716981132, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI87081951, Length=156, Percent_Identity=35.8974358974359, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081796, Length=165, Percent_Identity=30.9090909090909, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41549; Mature: 41418

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASKSARNVSLGAGLVALFAAVAGGATLIDRSGAAARDTPAPPAVAAAVATVETRPTMAA
CCCCCCCCEECHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHEECCCCCCCH
DEFSGRLEAVERVEIRSRVAGAVQEIRFREGALVTKGDLLVVIDPSLYAADVDRAQAQLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEECCCCEEHHHHHHHHHHH
SARARLTLAKADFERGQQLSSSSITQRELDTRANAYREAEASLRTAEAALKTAELNLGYT
HHHHEEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCE
NIRAPVSGRVGKVEITVGNLIAAGPSSPLLTTLVSVDPIYASFNADEQVVTRALKTLADE
EEECCCCCCCEEEEEEECCEEECCCCCCHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHC
RAPTAIDRIPVQMTTGAGGAPVKGRMQFIDNQVDPRSGTIRVRAVFDNADGRLMPGQFAR
CCCCHHHCCCEEEECCCCCCCCCHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCCCCHHC
LSMGQPKPQPALLVSERAVGTDQNKKFVMVVDARNQAEYREVTLGASVEGLRVVASGLKA
CCCCCCCCCCEEEEECCCCCCCCCCEEEEEEECCCCCCHHEEEECCCCHHHHHHHHHHCC
GERIVVNGLQRVRPGVTIKPETVAIDAASGSAVAQN
CCCHHHHHHHHHCCCCEECCCEEEEECCCCCCCCCC
>Mature Secondary Structure 
ASKSARNVSLGAGLVALFAAVAGGATLIDRSGAAARDTPAPPAVAAAVATVETRPTMAA
CCCCCCCEECHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHEECCCCCCCH
DEFSGRLEAVERVEIRSRVAGAVQEIRFREGALVTKGDLLVVIDPSLYAADVDRAQAQLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEECCCCEEHHHHHHHHHHH
SARARLTLAKADFERGQQLSSSSITQRELDTRANAYREAEASLRTAEAALKTAELNLGYT
HHHHEEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCE
NIRAPVSGRVGKVEITVGNLIAAGPSSPLLTTLVSVDPIYASFNADEQVVTRALKTLADE
EEECCCCCCCEEEEEEECCEEECCCCCCHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHC
RAPTAIDRIPVQMTTGAGGAPVKGRMQFIDNQVDPRSGTIRVRAVFDNADGRLMPGQFAR
CCCCHHHCCCEEEECCCCCCCCCHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCCCCHHC
LSMGQPKPQPALLVSERAVGTDQNKKFVMVVDARNQAEYREVTLGASVEGLRVVASGLKA
CCCCCCCCCCEEEEECCCCCCCCCCEEEEEEECCCCCCHHEEEECCCCHHHHHHHHHHCC
GERIVVNGLQRVRPGVTIKPETVAIDAASGSAVAQN
CCCHHHHHHHHHCCCCEECCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12271122 [H]