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The map label for this gene is livK [C]

Identifier: 86750376

GI number: 86750376

Start: 3735046

End: 3736269

Strand: Reverse

Name: livK [C]

Synonym: RPB_3265

Alternate gene names: 86750376

Gene position: 3736269-3735046 (Counterclockwise)

Preceding gene: 86750377

Following gene: 86750375

Centisome position: 70.08

GC content: 62.99

Gene sequence:

>1224_bases
ATGACGTCGCTCAGTCGTCGTTCGCTGATGCTTCGATCGGCCGCAGCCGGTCTCGCCGCGCTGACAAGCGCACGAAACGC
CCGCGCCGACGATTGGCCGGGCGTCACCGCGACCGAGCTCAAGATCGGATGCACGACCTCGCTGAGCGGCCCGGTGTCCG
CCTTGGGCACCATCGCGCGCAGCCAGGAAGCCTATTTCCGGATGCTGAACGATCAGGGCGGCATCGCCGGCCGCAAGATC
AACTTCATCATGTACGACGACGCGTTCAATCCCTCCAAGACGGTCGAGCAGACCCGGCGGCTGATCGAGAGCGACAATGT
CGCGTTCCTGTTTTCGATGCTCGGCACCGCGCCGAATTCGGCCGTGGTGAAATACATCAATGCCCAGAAGGTCCCGCATC
TGTTCCTGTCGGTGAACGGCGACAAATGGGGCGACTACAAGACCTATCCGTGGACGATGGGATTCGCGCCGAGTTCCCGG
ACCGAAGCCCAGGTGTTCGCCAAATACGCGCTCAGCCAGAAGCCGGATGCGAAGTTCGCGCTGCTGTATCAGAACGACGA
CCTCGGCAAGGATTTCGTCGCCGGCCTGCGCGACGTGCTGGGCGATCGCTACGACACGCTCGTGAAGGCATTGTCCTACG
AGGTCACCGAGCCGACGATCGACTCGCAGATCGTCACGCTGCGGGCGACCAATGCCGACGTGCTGATCTCGGGCGTCACG
TCGAAATTCGCCGCGCAGGCGATCCGCAAGGTCTACGAGTTGGGCTGGAAGCCGATGCACTTCGTCACCAGCGGCGCGGC
CTCCGTGGCGTCGACGATCATCCCGGTGGGCGCCGAGCGCGCCGAAGGCCTGATCACGTCGACCTATACCAAGGATCCGG
CCGACCCGGCATGGGCCGACGATCCCGGCATCAAGGACTACATGGCGTTCATGGCGAAATACTTCCCGACCGGAAACGCC
AAGGAAAGCCTCAATGCCTATGGCTACACCGCCGCGTCGGTGCTGCGCGTCCTGCTCGAACAGTGCAAAGGCAATTTCAG
CCGCGAGAGCATCATGGCGCAGGCCAACAACCTCAAGAACGTCGAGGTGCCCACGTTGTTGCCGGGCGTTCGGGTCAACA
CCAGCCCGACCAATCATCATCCGCTGCGGCAGATGCAGCTGCAGCGCTTTGAAGGGCAGGGCTACAAGCGCTTTGGCGCG
GTCATCGAGGGGACGATGCTGTAG

Upstream 100 bases:

>100_bases
GTATGGACGCAAGATAGCGTGCGCGACTGTCCCCTACGCTGCTACCTGATAACTGCGCGCAAGACGCAGACAGTTCCAGC
ACAACAGAGGGAGGGGAGCC

Downstream 100 bases:

>100_bases
GCGCGGCCACCATGGCGCAGCCGGAAGCTCGCAAGATGCTCGCGAGCGGCTGCGTTATGGGGTAGCCTGCCGACCGCCGT
TCCGATCGACCGGACGTCGA

Product: twin-arginine translocation pathway signal

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MTSLSRRSLMLRSAAAGLAALTSARNARADDWPGVTATELKIGCTTSLSGPVSALGTIARSQEAYFRMLNDQGGIAGRKI
NFIMYDDAFNPSKTVEQTRRLIESDNVAFLFSMLGTAPNSAVVKYINAQKVPHLFLSVNGDKWGDYKTYPWTMGFAPSSR
TEAQVFAKYALSQKPDAKFALLYQNDDLGKDFVAGLRDVLGDRYDTLVKALSYEVTEPTIDSQIVTLRATNADVLISGVT
SKFAAQAIRKVYELGWKPMHFVTSGAASVASTIIPVGAERAEGLITSTYTKDPADPAWADDPGIKDYMAFMAKYFPTGNA
KESLNAYGYTAASVLRVLLEQCKGNFSRESIMAQANNLKNVEVPTLLPGVRVNTSPTNHHPLRQMQLQRFEGQGYKRFGA
VIEGTML

Sequences:

>Translated_407_residues
MTSLSRRSLMLRSAAAGLAALTSARNARADDWPGVTATELKIGCTTSLSGPVSALGTIARSQEAYFRMLNDQGGIAGRKI
NFIMYDDAFNPSKTVEQTRRLIESDNVAFLFSMLGTAPNSAVVKYINAQKVPHLFLSVNGDKWGDYKTYPWTMGFAPSSR
TEAQVFAKYALSQKPDAKFALLYQNDDLGKDFVAGLRDVLGDRYDTLVKALSYEVTEPTIDSQIVTLRATNADVLISGVT
SKFAAQAIRKVYELGWKPMHFVTSGAASVASTIIPVGAERAEGLITSTYTKDPADPAWADDPGIKDYMAFMAKYFPTGNA
KESLNAYGYTAASVLRVLLEQCKGNFSRESIMAQANNLKNVEVPTLLPGVRVNTSPTNHHPLRQMQLQRFEGQGYKRFGA
VIEGTML
>Mature_406_residues
TSLSRRSLMLRSAAAGLAALTSARNARADDWPGVTATELKIGCTTSLSGPVSALGTIARSQEAYFRMLNDQGGIAGRKIN
FIMYDDAFNPSKTVEQTRRLIESDNVAFLFSMLGTAPNSAVVKYINAQKVPHLFLSVNGDKWGDYKTYPWTMGFAPSSRT
EAQVFAKYALSQKPDAKFALLYQNDDLGKDFVAGLRDVLGDRYDTLVKALSYEVTEPTIDSQIVTLRATNADVLISGVTS
KFAAQAIRKVYELGWKPMHFVTSGAASVASTIIPVGAERAEGLITSTYTKDPADPAWADDPGIKDYMAFMAKYFPTGNAK
ESLNAYGYTAASVLRVLLEQCKGNFSRESIMAQANNLKNVEVPTLLPGVRVNTSPTNHHPLRQMQLQRFEGQGYKRFGAV
IEGTML

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

None

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 44452; Mature: 44321

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLSRRSLMLRSAAAGLAALTSARNARADDWPGVTATELKIGCTTSLSGPVSALGTIAR
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHH
SQEAYFRMLNDQGGIAGRKINFIMYDDAFNPSKTVEQTRRLIESDNVAFLFSMLGTAPNS
HHHHHHHHHCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCH
AVVKYINAQKVPHLFLSVNGDKWGDYKTYPWTMGFAPSSRTEAQVFAKYALSQKPDAKFA
HHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCCCCCEE
LLYQNDDLGKDFVAGLRDVLGDRYDTLVKALSYEVTEPTIDSQIVTLRATNADVLISGVT
EEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEECHH
SKFAAQAIRKVYELGWKPMHFVTSGAASVASTIIPVGAERAEGLITSTYTKDPADPAWAD
HHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHCCCCHHHCCCEEECCCCCCCCCCCCC
DPGIKDYMAFMAKYFPTGNAKESLNAYGYTAASVLRVLLEQCKGNFSRESIMAQANNLKN
CCCHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCC
VEVPTLLPGVRVNTSPTNHHPLRQMQLQRFEGQGYKRFGAVIEGTML
CCCCCCCCCCEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
TSLSRRSLMLRSAAAGLAALTSARNARADDWPGVTATELKIGCTTSLSGPVSALGTIAR
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHH
SQEAYFRMLNDQGGIAGRKINFIMYDDAFNPSKTVEQTRRLIESDNVAFLFSMLGTAPNS
HHHHHHHHHCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCH
AVVKYINAQKVPHLFLSVNGDKWGDYKTYPWTMGFAPSSRTEAQVFAKYALSQKPDAKFA
HHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCCCCCEE
LLYQNDDLGKDFVAGLRDVLGDRYDTLVKALSYEVTEPTIDSQIVTLRATNADVLISGVT
EEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEECHH
SKFAAQAIRKVYELGWKPMHFVTSGAASVASTIIPVGAERAEGLITSTYTKDPADPAWAD
HHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHCCCCHHHCCCEEECCCCCCCCCCCCC
DPGIKDYMAFMAKYFPTGNAKESLNAYGYTAASVLRVLLEQCKGNFSRESIMAQANNLKN
CCCHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCC
VEVPTLLPGVRVNTSPTNHHPLRQMQLQRFEGQGYKRFGAVIEGTML
CCCCCCCCCCEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA