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The map label for this gene is caiD [C]

Identifier: 86750369

GI number: 86750369

Start: 3725769

End: 3726548

Strand: Reverse

Name: caiD [C]

Synonym: RPB_3258

Alternate gene names: 86750369

Gene position: 3726548-3725769 (Counterclockwise)

Preceding gene: 86750370

Following gene: 86750368

Centisome position: 69.89

GC content: 65.38

Gene sequence:

>780_bases
ATGAGCAATGACGCCGTGATCATCGAACAGCGCGATCAGGCGCTGTGGATCACCATCAACCGTCCCGACAAGCGCAACGC
CATCAATGCCGGTGTGGTCGAAGGCATCAGGACCGGCTGGAAGCAGGCGCATGACGATCCGTCGGTGCGCGTGATTGTAC
TGACCGGCGCCGGTGACAAGGCGTTCTGCGCCGGCGCCGACCTGCAATCCACCGGGGCCGCGTTCTCGTTCGACTTCTCC
AAGCCGAATGTCGACTACGCCGATCTGCTGCGGCTGGCGCAGAACGCCACCAAGCCGTCGATCGCCCGCGTCAACGGCAC
CTGCATGGCCGGCGGCATGGGCCTGCTGTGCATGACCGACATGGCGGTGGCGGCGGATCACGTCCTGTTCGGGCTGCCGG
AGGTCAAGGTCGGCGTGTTCCCGATGCAGGTGATGAGCCTGTTGCAGGACATCGCGCCGCGCCGGCTGATCAATGAATGG
GCGCTGACCGGCGAGCCGTTCGATGCGCCGACCGCGTGCGACGCGGGCCTGCTGAACTACGTGGTGCCGGGCGGCGAGCT
CGACGCCAAGGTCGACTGGCTGGTCAAGCGGCTGACCGACAAATCACCGACCGCGATCCGCCGCGGCAAATACGCGATGC
GGGCGATCGCGTCGATGTCGTTCGACGAGAGCATCGCCTACACCGAAAGCCAGATTGCGCTCTTGGCGATGACCGAAGAC
GCCAAAGAGGGCCTGAAGGCCTTCGCGGAGAAGCGCAAGCCGGTTTGGCCGGGGAAGTGA

Upstream 100 bases:

>100_bases
ATTGCGAGCGAAGCGAAGCAATCCAGAAGCTCCGTGCGCTGAGCTGGATTGCTTCGTCGCTTCGCTCCTCGCAATGACGA
GACCTGAGGACAGACCGACC

Downstream 100 bases:

>100_bases
CGCGCCGCTCGTTGCGGTCACGGTCCGGTTAACCGGACGTTATCGCCGCATATGGCCTATTCGGGGCGATTCTCCCGTGC
TGCGAGCCATGCCGTGACCC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MSNDAVIIEQRDQALWITINRPDKRNAINAGVVEGIRTGWKQAHDDPSVRVIVLTGAGDKAFCAGADLQSTGAAFSFDFS
KPNVDYADLLRLAQNATKPSIARVNGTCMAGGMGLLCMTDMAVAADHVLFGLPEVKVGVFPMQVMSLLQDIAPRRLINEW
ALTGEPFDAPTACDAGLLNYVVPGGELDAKVDWLVKRLTDKSPTAIRRGKYAMRAIASMSFDESIAYTESQIALLAMTED
AKEGLKAFAEKRKPVWPGK

Sequences:

>Translated_259_residues
MSNDAVIIEQRDQALWITINRPDKRNAINAGVVEGIRTGWKQAHDDPSVRVIVLTGAGDKAFCAGADLQSTGAAFSFDFS
KPNVDYADLLRLAQNATKPSIARVNGTCMAGGMGLLCMTDMAVAADHVLFGLPEVKVGVFPMQVMSLLQDIAPRRLINEW
ALTGEPFDAPTACDAGLLNYVVPGGELDAKVDWLVKRLTDKSPTAIRRGKYAMRAIASMSFDESIAYTESQIALLAMTED
AKEGLKAFAEKRKPVWPGK
>Mature_258_residues
SNDAVIIEQRDQALWITINRPDKRNAINAGVVEGIRTGWKQAHDDPSVRVIVLTGAGDKAFCAGADLQSTGAAFSFDFSK
PNVDYADLLRLAQNATKPSIARVNGTCMAGGMGLLCMTDMAVAADHVLFGLPEVKVGVFPMQVMSLLQDIAPRRLINEWA
LTGEPFDAPTACDAGLLNYVVPGGELDAKVDWLVKRLTDKSPTAIRRGKYAMRAIASMSFDESIAYTESQIALLAMTEDA
KEGLKAFAEKRKPVWPGK

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=255, Percent_Identity=30.9803921568627, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI31542718, Length=251, Percent_Identity=29.4820717131474, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI70995211, Length=266, Percent_Identity=27.4436090225564, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI37594469, Length=141, Percent_Identity=31.9148936170213, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI37594471, Length=141, Percent_Identity=31.9148936170213, Blast_Score=81, Evalue=9e-16,
Organism=Escherichia coli, GI221142681, Length=264, Percent_Identity=35.9848484848485, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1787659, Length=262, Percent_Identity=31.2977099236641, Blast_Score=120, Evalue=8e-29,
Organism=Escherichia coli, GI1788597, Length=244, Percent_Identity=33.1967213114754, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI87082183, Length=266, Percent_Identity=26.6917293233083, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787660, Length=249, Percent_Identity=28.9156626506024, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI25145438, Length=241, Percent_Identity=31.1203319502075, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17554946, Length=253, Percent_Identity=30.4347826086957, Blast_Score=107, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17549921, Length=218, Percent_Identity=27.0642201834862, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17535521, Length=256, Percent_Identity=26.171875, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17540714, Length=223, Percent_Identity=26.457399103139, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI25144160, Length=231, Percent_Identity=26.8398268398268, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI25144157, Length=231, Percent_Identity=26.8398268398268, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17560910, Length=264, Percent_Identity=26.5151515151515, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17540306, Length=232, Percent_Identity=25.4310344827586, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17534483, Length=230, Percent_Identity=24.3478260869565, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=212, Percent_Identity=32.5471698113208, Blast_Score=79, Evalue=5e-16,
Organism=Drosophila melanogaster, GI20129971, Length=239, Percent_Identity=31.7991631799163, Blast_Score=101, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24653477, Length=239, Percent_Identity=31.7991631799163, Blast_Score=101, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24653139, Length=266, Percent_Identity=30.0751879699248, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19922422, Length=260, Percent_Identity=25.3846153846154, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI28571730, Length=142, Percent_Identity=35.2112676056338, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28571729, Length=142, Percent_Identity=35.2112676056338, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24654903, Length=209, Percent_Identity=27.2727272727273, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21357171, Length=263, Percent_Identity=26.615969581749, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19920382, Length=258, Percent_Identity=23.6434108527132, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28017; Mature: 27885

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNDAVIIEQRDQALWITINRPDKRNAINAGVVEGIRTGWKQAHDDPSVRVIVLTGAGDK
CCCCEEEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
AFCAGADLQSTGAAFSFDFSKPNVDYADLLRLAQNATKPSIARVNGTCMAGGMGLLCMTD
EEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCEEEECCEEEECCCHHHHHHH
MAVAADHVLFGLPEVKVGVFPMQVMSLLQDIAPRRLINEWALTGEPFDAPTACDAGLLNY
HHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
VVPGGELDAKVDWLVKRLTDKSPTAIRRGKYAMRAIASMSFDESIAYTESQIALLAMTED
CCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCCEEEEEECHH
AKEGLKAFAEKRKPVWPGK
HHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SNDAVIIEQRDQALWITINRPDKRNAINAGVVEGIRTGWKQAHDDPSVRVIVLTGAGDK
CCCEEEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
AFCAGADLQSTGAAFSFDFSKPNVDYADLLRLAQNATKPSIARVNGTCMAGGMGLLCMTD
EEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCEEEECCEEEECCCHHHHHHH
MAVAADHVLFGLPEVKVGVFPMQVMSLLQDIAPRRLINEWALTGEPFDAPTACDAGLLNY
HHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
VVPGGELDAKVDWLVKRLTDKSPTAIRRGKYAMRAIASMSFDESIAYTESQIALLAMTED
CCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCCEEEEEECHH
AKEGLKAFAEKRKPVWPGK
HHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA