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The map label for this gene is gcvA [H]

Identifier: 86750249

GI number: 86750249

Start: 3587108

End: 3587998

Strand: Reverse

Name: gcvA [H]

Synonym: RPB_3138

Alternate gene names: 86750249

Gene position: 3587998-3587108 (Counterclockwise)

Preceding gene: 86750251

Following gene: 86750248

Centisome position: 67.3

GC content: 63.75

Gene sequence:

>891_bases
ATGACGCGCCGTCGTCTGCCGTCGTTGATTGCCCTGCGTGCCTTCGAGAGCGTCGGCCGGACCGGAAGCGTCCGTGCCGC
GGGCGACGAACTCGCCGTCAGCCATACCGTGGTGTCGCGTCATTTGCAGAACCTGCAGAAGTTCCTCGGAGTCGCGCTGG
TGCGCGGAGAGGGCCGCGGAGTCATCCTGACGGAAGCCGGTTTGAGTTTTCACCAGCAGATTTCTCAGGCATTCGACATC
ATCGCGCGTGCCACCATGAACGCCCGCCCGTCGTCGCTGCCGACCTTGAACATCTGGTGCATTCCCGGAATTGCCAATCG
CCTGTTGCTGTCGAGACTGCCTGAACTGACCGGCCCACCGCGCAACTGGGAAGTGAATTTGCAGCCGACCTTGACGCATC
CGGACTTGGTGCGGGCGGAGGCCGACGCCGAGATCGTCTATGCCGACGTGATCGATTCCCGCGGCTCGCTGATGGCGGAG
ATCCTGGTCCGGCCGCGCGTGTTTCCGGTCGCCAGCCCCGCTTATCTCGCGCGTTATCCGGCGATCGGCAGCCTCGACGA
TCTGTCGAAGATCGCATTGATCCACGAGGAGTCCACCGAGCAATGGACGCGCTGGTTCGAACTCGCGGGCCTCGACGATC
TGCCGCCGCTGCGCGGTCAACGGCTGTGGCACGCCCATCTGGCGATCGAAGCGGCGCGACTCGGGCAGGGGGTCGCGCTG
GGTAACGAAGTCCTGGTCGAAGAGGATCTGCGCTCAGGCGCATTGGTGGAGGTGCTCAATTCGTCGGTCTATATGGAGAC
TTATCAGCTACTGGCGCTGAAGGAGCGCTGGGACGATCCGGAGATCGTCGCGTTGCGCAATTGGCTGAGACGCGTTCTGG
CCGGGCACTAG

Upstream 100 bases:

>100_bases
GCTCAAATTTCGCCCGACCCGCGAAACCTGCGAGAGCGCGGAGAAGACAAGGAGATAATGTGCAAGCGTGCCGTTACACC
GACCCGTTGATGGAGCGTTC

Downstream 100 bases:

>100_bases
GCGACGCAGCGATCGGCGCTTCACTGGCTGCGTGTTGAGCAGATCGCCCAATAAACGGACATCATCTGCTCGCGATCGTG
CAGCTCCGATCGATTGGTGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MTRRRLPSLIALRAFESVGRTGSVRAAGDELAVSHTVVSRHLQNLQKFLGVALVRGEGRGVILTEAGLSFHQQISQAFDI
IARATMNARPSSLPTLNIWCIPGIANRLLLSRLPELTGPPRNWEVNLQPTLTHPDLVRAEADAEIVYADVIDSRGSLMAE
ILVRPRVFPVASPAYLARYPAIGSLDDLSKIALIHEESTEQWTRWFELAGLDDLPPLRGQRLWHAHLAIEAARLGQGVAL
GNEVLVEEDLRSGALVEVLNSSVYMETYQLLALKERWDDPEIVALRNWLRRVLAGH

Sequences:

>Translated_296_residues
MTRRRLPSLIALRAFESVGRTGSVRAAGDELAVSHTVVSRHLQNLQKFLGVALVRGEGRGVILTEAGLSFHQQISQAFDI
IARATMNARPSSLPTLNIWCIPGIANRLLLSRLPELTGPPRNWEVNLQPTLTHPDLVRAEADAEIVYADVIDSRGSLMAE
ILVRPRVFPVASPAYLARYPAIGSLDDLSKIALIHEESTEQWTRWFELAGLDDLPPLRGQRLWHAHLAIEAARLGQGVAL
GNEVLVEEDLRSGALVEVLNSSVYMETYQLLALKERWDDPEIVALRNWLRRVLAGH
>Mature_295_residues
TRRRLPSLIALRAFESVGRTGSVRAAGDELAVSHTVVSRHLQNLQKFLGVALVRGEGRGVILTEAGLSFHQQISQAFDII
ARATMNARPSSLPTLNIWCIPGIANRLLLSRLPELTGPPRNWEVNLQPTLTHPDLVRAEADAEIVYADVIDSRGSLMAEI
LVRPRVFPVASPAYLARYPAIGSLDDLSKIALIHEESTEQWTRWFELAGLDDLPPLRGQRLWHAHLAIEAARLGQGVALG
NEVLVEEDLRSGALVEVLNSSVYMETYQLLALKERWDDPEIVALRNWLRRVLAGH

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=292, Percent_Identity=30.1369863013699, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI1788706, Length=293, Percent_Identity=26.6211604095563, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1786448, Length=254, Percent_Identity=28.3464566929134, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32869; Mature: 32738

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRRRLPSLIALRAFESVGRTGSVRAAGDELAVSHTVVSRHLQNLQKFLGVALVRGEGRG
CCCHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCEEEEECCCCE
VILTEAGLSFHQQISQAFDIIARATMNARPSSLPTLNIWCIPGIANRLLLSRLPELTGPP
EEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHCHHHCCCC
RNWEVNLQPTLTHPDLVRAEADAEIVYADVIDSRGSLMAEILVRPRVFPVASPAYLARYP
CCEEEEEEECCCCCHHHCCCCCCEEEEEEHHCCCHHHHHHHHHCCCCCCCCCCHHHHHCC
AIGSLDDLSKIALIHEESTEQWTRWFELAGLDDLPPLRGQRLWHAHLAIEAARLGQGVAL
CCCCHHHHHHHHEEECCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
GNEVLVEEDLRSGALVEVLNSSVYMETYQLLALKERWDDPEIVALRNWLRRVLAGH
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TRRRLPSLIALRAFESVGRTGSVRAAGDELAVSHTVVSRHLQNLQKFLGVALVRGEGRG
CCHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCEEEEECCCCE
VILTEAGLSFHQQISQAFDIIARATMNARPSSLPTLNIWCIPGIANRLLLSRLPELTGPP
EEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHCHHHCCCC
RNWEVNLQPTLTHPDLVRAEADAEIVYADVIDSRGSLMAEILVRPRVFPVASPAYLARYP
CCEEEEEEECCCCCHHHCCCCCCEEEEEEHHCCCHHHHHHHHHCCCCCCCCCCHHHHHCC
AIGSLDDLSKIALIHEESTEQWTRWFELAGLDDLPPLRGQRLWHAHLAIEAARLGQGVAL
CCCCHHHHHHHHEEECCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
GNEVLVEEDLRSGALVEVLNSSVYMETYQLLALKERWDDPEIVALRNWLRRVLAGH
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]