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The map label for this gene is cocE [H]

Identifier: 86750227

GI number: 86750227

Start: 3548179

End: 3549861

Strand: Reverse

Name: cocE [H]

Synonym: RPB_3114

Alternate gene names: 86750227

Gene position: 3549861-3548179 (Counterclockwise)

Preceding gene: 86750228

Following gene: 86750226

Centisome position: 66.58

GC content: 66.31

Gene sequence:

>1683_bases
ATGATCGTTGAGAAGAACGTCGTCATTCCGGTCAGCGACGGACATTGGCTCCGTGCCAATATCTATCGTCCCGCCCAGGA
CGGTCCGTATCCGGTGCTGATGTCGCTCGGCATCTACGGCAAGGACATCCACTTCGCCGACGGCTACGAACTGCCGTGGA
AGAAGTTGCAGACGCTCAATCCCGCGATCATCGACGACGGATCGAGCGGACGCTATTTGCGCTGGGAAATGATCGACCCC
GAGCGCTGGGTGCCGGACGGCTATGTGGTGATCGCGGTCGATGGCCGCGGCAGCGGACAGTCGCCCGGCTATCTCGACCC
GTTCTCGCCGCGCGAGACGCAGGACTATTACGACGCGATCGAATGGGCCGCGACGCAGCCGTGGTCGTGCGGCAAGGTCG
GCCTGATCGGTATTTCGTATCTGTCGATCAAGCAGTGGCAGGTTGCCGCGCTGCAGCCGCCCCATCTCGCGGCGATCTGC
CCGTGGGAGGGCGGTAGCGACCTGTATCGCGACTGGAGTCACCACGGCGGCATCTTCAGCAATTCGTTCCCGACCGCGTG
GTGGCCACGCCAGGTGCTGCCCAATCAGCACGGCAACGCGGCGACGCACCATCGCGATCGCGACACCGGCAAGACCACCA
CCGGCGCGGCGCTGAGCGACTATCTGCTGCGCGCCAACCGCGCCGATCATGCCGGCGATCTGTTGCAGCATCCGCTCGAC
GATGCCTGGTTCCGGCAGCGTTCGCCGGATCTCGGCCGCGTTCAGGTGCCGCTGTTCTCCGCCGGCAATTGGGGGGGCTG
CGGCCTGCATTTGCGCGGCAACGTCGAAGGCTTCTGTCGCGCCGGCTCGGGGTTGAAATGGCTCGATATGCATATTGGCA
CGCATTTCGAGAGCTTCTATCTGCCGGCCTATATCGACCGCCAGAAGCGGTTTTTCGCGTATTTTCTCAAGGGTGAGGCT
AACGGTTGGGATGGCGAGCCGACGATCCGGCTGGAGATCCGCAGCCCCGACGGCGCGGTTGCGCGAAACGAGGCGGAGTG
GCCGCTGGCGCGCACCGACTGGCAGCTGCACTATCTCGACGCAGCGGCGCAGAGCTTGTCGATCGATTCGCCGGCCGCGG
CAGCATCGAGCACCTTCGTCGCGATGGGCGACGGCGTCACCTTCCTCACGCGGCCGTTCGATCAGGCCACTGAGTTCACG
GGGCCGGTGACGGCGCGGCTGTGGATCGCGTCGTCGACGCCGGACGCGGACATCTTCGCGACGCTGCGGCTGTTTCGGCC
GGACGGCCGCGAAGTGGTGTTCGCCGGCGCCAGCGACACCGAGCCGGTGGCGCGCGGCTGGCTGCGCGCGTCGCATCGCA
AGCTCGATCCGGCGATGTCAACGCCGGTGCGGCCGTATCACAGCCACGACGAGATCCAGAAGCTCGAGCCGGGCCAGCCG
TATCTGCTCGACGTCGAGATCTGGCCGACCAGCATCGTTTGCCCGCCGGGCTATCGGTTGGCGCTGACGCTGCAGGGGCG
GGACTACGAGGCGCCCGGCGTCTCCGGCCGCATCCTGCACAATCACGAACGCGATCGCCCGCGCACCGAGTTCGACGGCC
GCACCACGATCCTCACGGGACCCGGCCACGTGTCCTATCTGCTGCTGCCGCACATCCCGTCGCAGTCCGGAGCCACGCCA
TGA

Upstream 100 bases:

>100_bases
CGATCGCGCGCGACATCAATCGTGCGCTGGCTCCGATGTGGGGAGCCGCGCAAGTCGCGGCGGGTTCGCCGGCAGGCGAA
CCAAAACGGCAAGGTTGCGC

Downstream 100 bases:

>100_bases
AGATGCCGCCGTTCGACTATGCGTGCCCGGCGACGCTGGACGAGGCGGTGCGGCTGCTCGCCGCCAACGACGGCGCCAAG
GTGCTGTCCGGCGGCCAGAG

Product: peptidase S15

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 560; Mature: 560

Protein sequence:

>560_residues
MIVEKNVVIPVSDGHWLRANIYRPAQDGPYPVLMSLGIYGKDIHFADGYELPWKKLQTLNPAIIDDGSSGRYLRWEMIDP
ERWVPDGYVVIAVDGRGSGQSPGYLDPFSPRETQDYYDAIEWAATQPWSCGKVGLIGISYLSIKQWQVAALQPPHLAAIC
PWEGGSDLYRDWSHHGGIFSNSFPTAWWPRQVLPNQHGNAATHHRDRDTGKTTTGAALSDYLLRANRADHAGDLLQHPLD
DAWFRQRSPDLGRVQVPLFSAGNWGGCGLHLRGNVEGFCRAGSGLKWLDMHIGTHFESFYLPAYIDRQKRFFAYFLKGEA
NGWDGEPTIRLEIRSPDGAVARNEAEWPLARTDWQLHYLDAAAQSLSIDSPAAAASSTFVAMGDGVTFLTRPFDQATEFT
GPVTARLWIASSTPDADIFATLRLFRPDGREVVFAGASDTEPVARGWLRASHRKLDPAMSTPVRPYHSHDEIQKLEPGQP
YLLDVEIWPTSIVCPPGYRLALTLQGRDYEAPGVSGRILHNHERDRPRTEFDGRTTILTGPGHVSYLLLPHIPSQSGATP

Sequences:

>Translated_560_residues
MIVEKNVVIPVSDGHWLRANIYRPAQDGPYPVLMSLGIYGKDIHFADGYELPWKKLQTLNPAIIDDGSSGRYLRWEMIDP
ERWVPDGYVVIAVDGRGSGQSPGYLDPFSPRETQDYYDAIEWAATQPWSCGKVGLIGISYLSIKQWQVAALQPPHLAAIC
PWEGGSDLYRDWSHHGGIFSNSFPTAWWPRQVLPNQHGNAATHHRDRDTGKTTTGAALSDYLLRANRADHAGDLLQHPLD
DAWFRQRSPDLGRVQVPLFSAGNWGGCGLHLRGNVEGFCRAGSGLKWLDMHIGTHFESFYLPAYIDRQKRFFAYFLKGEA
NGWDGEPTIRLEIRSPDGAVARNEAEWPLARTDWQLHYLDAAAQSLSIDSPAAAASSTFVAMGDGVTFLTRPFDQATEFT
GPVTARLWIASSTPDADIFATLRLFRPDGREVVFAGASDTEPVARGWLRASHRKLDPAMSTPVRPYHSHDEIQKLEPGQP
YLLDVEIWPTSIVCPPGYRLALTLQGRDYEAPGVSGRILHNHERDRPRTEFDGRTTILTGPGHVSYLLLPHIPSQSGATP
>Mature_560_residues
MIVEKNVVIPVSDGHWLRANIYRPAQDGPYPVLMSLGIYGKDIHFADGYELPWKKLQTLNPAIIDDGSSGRYLRWEMIDP
ERWVPDGYVVIAVDGRGSGQSPGYLDPFSPRETQDYYDAIEWAATQPWSCGKVGLIGISYLSIKQWQVAALQPPHLAAIC
PWEGGSDLYRDWSHHGGIFSNSFPTAWWPRQVLPNQHGNAATHHRDRDTGKTTTGAALSDYLLRANRADHAGDLLQHPLD
DAWFRQRSPDLGRVQVPLFSAGNWGGCGLHLRGNVEGFCRAGSGLKWLDMHIGTHFESFYLPAYIDRQKRFFAYFLKGEA
NGWDGEPTIRLEIRSPDGAVARNEAEWPLARTDWQLHYLDAAAQSLSIDSPAAAASSTFVAMGDGVTFLTRPFDQATEFT
GPVTARLWIASSTPDADIFATLRLFRPDGREVVFAGASDTEPVARGWLRASHRKLDPAMSTPVRPYHSHDEIQKLEPGQP
YLLDVEIWPTSIVCPPGYRLALTLQGRDYEAPGVSGRILHNHERDRPRTEFDGRTTILTGPGHVSYLLLPHIPSQSGATP

Specific function: Hydrolyzes cocaine to benzoate and ecgonine methyl ester, endowing the bacteria with the ability to utilize cocaine as a sole source of carbon and energy for growth, as this bacterium lives in the rhizosphere of coca plants [H]

COG id: COG2936

COG function: function code R; Predicted acyl esterases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha/beta hydrolase family. Carboxylesterase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005674
- InterPro:   IPR008979
- InterPro:   IPR013736
- InterPro:   IPR000383 [H]

Pfam domain/function: PF02129 Peptidase_S15; PF08530 PepX_C [H]

EC number: NA

Molecular weight: Translated: 62361; Mature: 62361

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVEKNVVIPVSDGHWLRANIYRPAQDGPYPVLMSLGIYGKDIHFADGYELPWKKLQTLN
CEEECCEEEECCCCCEEEEEEECCCCCCCEEEEEECCCCCCEEEECCCCCCCHHHHHCCC
PAIIDDGSSGRYLRWEMIDPERWVPDGYVVIAVDGRGSGQSPGYLDPFSPRETQDYYDAI
CEEEECCCCCCEEEEEECCCHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHH
EWAATQPWSCGKVGLIGISYLSIKQWQVAALQPPHLAAICPWEGGSDLYRDWSHHGGIFS
HHHCCCCCCCCCEEEEEEEEEEEECEEEEECCCCCEEEEECCCCCHHHHHHHHHCCCEEC
NSFPTAWWPRQVLPNQHGNAATHHRDRDTGKTTTGAALSDYLLRANRADHAGDLLQHPLD
CCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHCCCC
DAWFRQRSPDLGRVQVPLFSAGNWGGCGLHLRGNVEGFCRAGSGLKWLDMHIGTHFESFY
HHHHHHCCCCCCEEEEEEEECCCCCCEEEEEECCCCEEEECCCCCEEEEEECCCCHHHEE
LPAYIDRQKRFFAYFLKGEANGWDGEPTIRLEIRSPDGAVARNEAEWPLARTDWQLHYLD
CCCEECCCCEEEEEEEEECCCCCCCCCEEEEEEECCCCCEECCCCCCCEECCCCEEEEEE
AAAQSLSIDSPAAAASSTFVAMGDGVTFLTRPFDQATEFTGPVTARLWIASSTPDADIFA
HHHHHCCCCCCCCCCCCEEEEECCCEEEEECCCHHHHHCCCCEEEEEEEECCCCCCCEEE
TLRLFRPDGREVVFAGASDTEPVARGWLRASHRKLDPAMSTPVRPYHSHDEIQKLEPGQP
EEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHCCHHHCCCCCCCCCCHHHHHCCCCCC
YLLDVEIWPTSIVCPPGYRLALTLQGRDYEAPGVSGRILHNHERDRPRTEFDGRTTILTG
EEEEEEECCCEEECCCCCEEEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEC
PGHVSYLLLPHIPSQSGATP
CCCEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MIVEKNVVIPVSDGHWLRANIYRPAQDGPYPVLMSLGIYGKDIHFADGYELPWKKLQTLN
CEEECCEEEECCCCCEEEEEEECCCCCCCEEEEEECCCCCCEEEECCCCCCCHHHHHCCC
PAIIDDGSSGRYLRWEMIDPERWVPDGYVVIAVDGRGSGQSPGYLDPFSPRETQDYYDAI
CEEEECCCCCCEEEEEECCCHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHH
EWAATQPWSCGKVGLIGISYLSIKQWQVAALQPPHLAAICPWEGGSDLYRDWSHHGGIFS
HHHCCCCCCCCCEEEEEEEEEEEECEEEEECCCCCEEEEECCCCCHHHHHHHHHCCCEEC
NSFPTAWWPRQVLPNQHGNAATHHRDRDTGKTTTGAALSDYLLRANRADHAGDLLQHPLD
CCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHCCCC
DAWFRQRSPDLGRVQVPLFSAGNWGGCGLHLRGNVEGFCRAGSGLKWLDMHIGTHFESFY
HHHHHHCCCCCCEEEEEEEECCCCCCEEEEEECCCCEEEECCCCCEEEEEECCCCHHHEE
LPAYIDRQKRFFAYFLKGEANGWDGEPTIRLEIRSPDGAVARNEAEWPLARTDWQLHYLD
CCCEECCCCEEEEEEEEECCCCCCCCCEEEEEEECCCCCEECCCCCCCEECCCCEEEEEE
AAAQSLSIDSPAAAASSTFVAMGDGVTFLTRPFDQATEFTGPVTARLWIASSTPDADIFA
HHHHHCCCCCCCCCCCCEEEEECCCEEEEECCCHHHHHCCCCEEEEEEEECCCCCCCEEE
TLRLFRPDGREVVFAGASDTEPVARGWLRASHRKLDPAMSTPVRPYHSHDEIQKLEPGQP
EEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHCCHHHCCCCCCCCCCHHHHHCCCCCC
YLLDVEIWPTSIVCPPGYRLALTLQGRDYEAPGVSGRILHNHERDRPRTEFDGRTTILTG
EEEEEEECCCEEECCCCCEEEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEC
PGHVSYLLLPHIPSQSGATP
CCCEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10698749; 11742345; 12369817 [H]