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The map label for this gene is dctA

Identifier: 86750127

GI number: 86750127

Start: 3432990

End: 3434327

Strand: Reverse

Name: dctA

Synonym: RPB_3010

Alternate gene names: 86750127

Gene position: 3434327-3432990 (Counterclockwise)

Preceding gene: 86750128

Following gene: 86750125

Centisome position: 64.41

GC content: 63.38

Gene sequence:

>1338_bases
ATGTCGACGATGACCGATGTCGGCGTTCCTGAAACGTCGCGACCGTCCAACGCCAAGCCTTGGTACAAGGTGCTCTATAT
CCAGGTCTTGATCGCGATCGTGCTCGGCGTGCTGGTCGGCTGGCTGTCTCCGCATCTGGCGACCAATCCGTGGATCAAGG
CGCTCGGCGACGGATTCGTCAAACTGATCAAGATGGTGATAGCGCCGATCATCTTCTGCACGGTCGTCTCCGGCATCGCG
CATATCCAGGACGCCCGCAAGGTCGGCCGGGTGGGCATCAAGGCACTGGTGTATTTCGAAGTGGTGTCGTCGTTCGCGCT
GATCCTCGGTCTCGTCGTCGGCAATCTTCTGCCGGTCGGGCATGGGCTCGCAGCCAAGCCGGACGCCGGAGCCGTGGCGA
AGTACGTCGACCAGGCCAGCCACATGCACGCGGTCGACTTCTTTCTCAACATCATTCCCGAGAGCGTCGTCGGCGCGTTC
GCGAAGGGCGACATCCTGCAGGTGCTGCTGTTCGCCATCCTGTTCGGCTTCGCGCTGATGGCGCTCGGTGAGCGCGGGCA
TCGGCTGCGCGACGTGATCGACGACACCGCTCATGCGGTGTTCGGCGTGATCGCGATCGTGATGAAGGCCGCGCCGGTCG
GTGCCTTCGGCGCGATGGCCTTCACCATCGGCAAATACGGCCCGGCCGCGCTCGGCAATCTGATCGGCCTGGTCGCGCTG
TTCTATGCGACCGCGGCGTTGTTCGTGTTCGTGGTGCTGGGGGTGATCGCCAAATTCGTCGGCTTCAACATCTTCAAGTT
CCTCGGCTACATCAAGGACGAGCTGTTGATCGTGCTCGGCACCTCGTCGTCCGAGAGCGCGCTGCCGCAACTGATGGAGA
AGCTCGAGCGGCTGGGCTGCTCGAAGTCGGTTGTGGGCCTGGTGGTGCCGACCGGATACTCGTTCAATCTCGACGGCACC
AACATCTACATGACGCTGGCGACGCTGTTCATCGCGCAGGCGCTCGGCATCGAGCTGTCGTTCTCCGAACAGGTCACGAT
CCTGCTGGTTGCGATGCTGACCTCGAAGGGCGCCAGCGGCGTCACCGGCGCTGGTTTCGTCACGCTGGCGGGGACGCTCG
CCGCGGTCAATCCGGCTCTGGTGCCGGGCATGGCGATCGTATTCTCGATCGACAAGTTCATGAGCGAGGTGCGCGCGCTC
ACCAACATCACCGGCAACGGCGTCGCCACCGTGTTCGTGTCGTGGTGGGAGGGCGAGCTCGACCACGATCGGCTGCACGC
CAATCTCGACAAGACGATCGACCCGTCGGACGTCGAGACTGCGGTCACCACCGGCTGA

Upstream 100 bases:

>100_bases
GGCTCCGTTCAATTCTGAATCGATTTGGCCGACCGGTCTGCCTGCGGTCGTCCCGACGTCGTCGTCAAGACAGGCACATC
GAGACCATTGGAGTGATGCC

Downstream 100 bases:

>100_bases
TCGGTCGTTGCTGCCTGAAACGAAAACCGCCCGGCCTGATGGCCGGGCGGTTTCTTGTCGAGTTGACTGCGGCTGATCAG
TTGCCGCCGCCGAAGCGGCG

Product: Sodium:dicarboxylate symporter

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MSTMTDVGVPETSRPSNAKPWYKVLYIQVLIAIVLGVLVGWLSPHLATNPWIKALGDGFVKLIKMVIAPIIFCTVVSGIA
HIQDARKVGRVGIKALVYFEVVSSFALILGLVVGNLLPVGHGLAAKPDAGAVAKYVDQASHMHAVDFFLNIIPESVVGAF
AKGDILQVLLFAILFGFALMALGERGHRLRDVIDDTAHAVFGVIAIVMKAAPVGAFGAMAFTIGKYGPAALGNLIGLVAL
FYATAALFVFVVLGVIAKFVGFNIFKFLGYIKDELLIVLGTSSSESALPQLMEKLERLGCSKSVVGLVVPTGYSFNLDGT
NIYMTLATLFIAQALGIELSFSEQVTILLVAMLTSKGASGVTGAGFVTLAGTLAAVNPALVPGMAIVFSIDKFMSEVRAL
TNITGNGVATVFVSWWEGELDHDRLHANLDKTIDPSDVETAVTTG

Sequences:

>Translated_445_residues
MSTMTDVGVPETSRPSNAKPWYKVLYIQVLIAIVLGVLVGWLSPHLATNPWIKALGDGFVKLIKMVIAPIIFCTVVSGIA
HIQDARKVGRVGIKALVYFEVVSSFALILGLVVGNLLPVGHGLAAKPDAGAVAKYVDQASHMHAVDFFLNIIPESVVGAF
AKGDILQVLLFAILFGFALMALGERGHRLRDVIDDTAHAVFGVIAIVMKAAPVGAFGAMAFTIGKYGPAALGNLIGLVAL
FYATAALFVFVVLGVIAKFVGFNIFKFLGYIKDELLIVLGTSSSESALPQLMEKLERLGCSKSVVGLVVPTGYSFNLDGT
NIYMTLATLFIAQALGIELSFSEQVTILLVAMLTSKGASGVTGAGFVTLAGTLAAVNPALVPGMAIVFSIDKFMSEVRAL
TNITGNGVATVFVSWWEGELDHDRLHANLDKTIDPSDVETAVTTG
>Mature_444_residues
STMTDVGVPETSRPSNAKPWYKVLYIQVLIAIVLGVLVGWLSPHLATNPWIKALGDGFVKLIKMVIAPIIFCTVVSGIAH
IQDARKVGRVGIKALVYFEVVSSFALILGLVVGNLLPVGHGLAAKPDAGAVAKYVDQASHMHAVDFFLNIIPESVVGAFA
KGDILQVLLFAILFGFALMALGERGHRLRDVIDDTAHAVFGVIAIVMKAAPVGAFGAMAFTIGKYGPAALGNLIGLVALF
YATAALFVFVVLGVIAKFVGFNIFKFLGYIKDELLIVLGTSSSESALPQLMEKLERLGCSKSVVGLVVPTGYSFNLDGTN
IYMTLATLFIAQALGIELSFSEQVTILLVAMLTSKGASGVTGAGFVTLAGTLAAVNPALVPGMAIVFSIDKFMSEVRALT
NITGNGVATVFVSWWEGELDHDRLHANLDKTIDPSDVETAVTTG

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family

Homologues:

Organism=Homo sapiens, GI169790839, Length=263, Percent_Identity=31.1787072243346, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI4827012, Length=272, Percent_Identity=30.1470588235294, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI40254478, Length=448, Percent_Identity=24.5535714285714, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI5032093, Length=413, Percent_Identity=27.8450363196126, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI66773030, Length=474, Percent_Identity=24.2616033755274, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI21314632, Length=400, Percent_Identity=26, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI223468566, Length=296, Percent_Identity=29.7297297297297, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI223468564, Length=288, Percent_Identity=29.5138888888889, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI194239697, Length=464, Percent_Identity=24.1379310344828, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI262359914, Length=222, Percent_Identity=31.5315315315315, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI301601644, Length=182, Percent_Identity=27.4725274725275, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI1789947, Length=413, Percent_Identity=56.9007263922518, Blast_Score=463, Evalue=1e-131,
Organism=Escherichia coli, GI1790514, Length=428, Percent_Identity=36.9158878504673, Blast_Score=275, Evalue=6e-75,
Organism=Escherichia coli, GI1788024, Length=400, Percent_Identity=22.5, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1789473, Length=319, Percent_Identity=25.0783699059561, Blast_Score=73, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17537407, Length=459, Percent_Identity=25.7080610021786, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71983099, Length=467, Percent_Identity=26.9807280513919, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI71983106, Length=467, Percent_Identity=26.9807280513919, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI71996953, Length=465, Percent_Identity=26.2365591397849, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI193206654, Length=416, Percent_Identity=24.0384615384615, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI193206505, Length=459, Percent_Identity=24.1830065359477, Blast_Score=98, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17541374, Length=436, Percent_Identity=22.7064220183486, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24583025, Length=460, Percent_Identity=23.2608695652174, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI17137668, Length=460, Percent_Identity=23.2608695652174, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24583023, Length=460, Percent_Identity=23.2608695652174, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI281360481, Length=456, Percent_Identity=25, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI281360483, Length=456, Percent_Identity=25, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI17137666, Length=456, Percent_Identity=25, Blast_Score=92, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DCTA_RHOP2 (Q2IVP8)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_486623.1
- ProteinModelPortal:   Q2IVP8
- SMR:   Q2IVP8
- STRING:   Q2IVP8
- GeneID:   3910809
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_3010
- eggNOG:   COG1301
- HOGENOM:   HBG739804
- OMA:   ALATDMK
- ProtClustDB:   CLSK818651
- BioCyc:   RPAL316058:RPB_3010-MONOMER
- HAMAP:   MF_01300
- InterPro:   IPR001991
- InterPro:   IPR018107
- PANTHER:   PTHR11958
- PRINTS:   PR00173

Pfam domain/function: PF00375 SDF

EC number: NA

Molecular weight: Translated: 46984; Mature: 46853

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1e2d40bc)-; HASH(0x1ed412a4)-; HASH(0x1edccd18)-; HASH(0x1eb47254)-; HASH(0x1e9ad304)-; HASH(0x1ece810c)-; HASH(0x1b833350)-; HASH(0x1ebc2698)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CHHHHHCCCCCCCCCCHHCHHCCCH
>Mature Secondary Structure 
STMTDVGVPETSRPSNAKPWYKVLYIQVLIAIVLGVLVGWLSPHLATNPWIKALGDGFV
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
KLIKMVIAPIIFCTVVSGIAHIQDARKVGRVGIKALVYFEVVSSFALILGLVVGNLLPVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
HGLAAKPDAGAVAKYVDQASHMHAVDFFLNIIPESVVGAFAKGDILQVLLFAILFGFALM
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
ALGERGHRLRDVIDDTAHAVFGVIAIVMKAAPVGAFGAMAFTIGKYGPAALGNLIGLVAL
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
FYATAALFVFVVLGVIAKFVGFNIFKFLGYIKDELLIVLGTSSSESALPQLMEKLERLGC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHCCC
SKSVVGLVVPTGYSFNLDGTNIYMTLATLFIAQALGIELSFSEQVTILLVAMLTSKGASG
CCCEEEEEEECCEEECCCCCHHHHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHCCCCCC
VTGAGFVTLAGTLAAVNPALVPGMAIVFSIDKFMSEVRALTNITGNGVATVFVSWWEGEL
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCC
DHDRLHANLDKTIDPSDVETAVTTG
CHHHHHCCCCCCCCCCHHCHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA