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The map label for this gene is yjeA [H]

Identifier: 86750072

GI number: 86750072

Start: 3364318

End: 3365373

Strand: Reverse

Name: yjeA [H]

Synonym: RPB_2955

Alternate gene names: 86750072

Gene position: 3365373-3364318 (Counterclockwise)

Preceding gene: 86750078

Following gene: 86750071

Centisome position: 63.12

GC content: 69.89

Gene sequence:

>1056_bases
ATGCACAAAGAGGCCGGCGCGTCGCCCTGGTGGAATCGGGACAGGTATCTGGACCGACAGCCTTTTTTGCGCGCGCGGGG
CGCCGTGACCCGGGCGGCCCGGGACTGGTTCGCGACCGCGGATTTCGCCGAGGTGGAGACCGCGATCCTGCAACTGTCGC
CCGGCAACGAGACTCATCTGCACGCGCCGCGTACCGAACTCAAGGGCCCCGACGGCGCGCGGCGGACGCGATACCTGCGC
ACCTCGCCGGAATTCGCCTGCAAGAAGCTATTGGCCGCCGGCGAGCAGCGGATCTTCGAATTCGCCCGGGTGTTCCGCGA
CCGCGAGCGCGGGCGGTTGCATTTGCCGGAATTCACCATGCTGGAATGGTACCGGGCCTGCGAGCGCTATGAGGCGGTGA
TCGCCGATTGCGTCGCGCTGATCGCGCTGGCGGCGCGCACCACGGGAATCGGCGGCTTCACGTTGCGGGACCGGAGCTGC
GATCCGCAGGCGGAGGCCGAGTTGCTGACGGTGGCCGCCGCGTTCGAGCGCTTCGCCGCGATCGATCTGCTGGCGACCAT
CATCGACGGCGAGGGCGATCGCGACGCGCTGGCGCGCGCGGCCGCCGCGAAAGTGCGCGTCGCCACCGACGACAATTGGT
CGGATATTTTCAGCAAGGTGCTGGTCGAGCATGTCGAGCCGCGGCTCGGCGAGGGGCGGATGACGGTGCTGTGCGATTAT
CCGGCGCCGGAGGCGGCGCTGGCGCGGACCAAGGCCGGCGACCCGCGGGTCGCCGAGCGCTTCGAGGTCTATGCCTGCGG
CGTCGAACTCGCCAACGGCTTCGGCGAACTGACTGATGCCGCCGAGCAGCGCGCGCGCTTCACCGCGGCGATGGACGAGA
AGCAGCGCCGCTATGGCGAGCGCTATCCGCTCGACGAGGATTTCCTCGCCGCGGTCGGACAGATGCCCGAGGCGAGCGGC
GTGGCGCTCGGCTTCGACCGGCTGGTGATGCTGGCGGCGGGCGCGACCAGCATCGATCAGGTGGTGTGGACGCCGCCGGC
GGGCGAGGCGTCATGA

Upstream 100 bases:

>100_bases
CATTGGCATCCGGCGGCCTGCGAAACGGTTTCGGGCCGCAACATACCCATTTGCCCCGACTATGCCAGCGTTTTGCGCTT
TCCGGCGGGGGCGACTTGAG

Downstream 100 bases:

>100_bases
GCAGGGTCACACGATTGCAAACGCCCGCCGCCACAACACTGCGGCAACCCTCTGAGCTGATCGCGCAAGGTCTCGCGCCG
GCGGAGTCGCGCGACGATCT

Product: tRNA synthetase, class II (D, K and N)

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MHKEAGASPWWNRDRYLDRQPFLRARGAVTRAARDWFATADFAEVETAILQLSPGNETHLHAPRTELKGPDGARRTRYLR
TSPEFACKKLLAAGEQRIFEFARVFRDRERGRLHLPEFTMLEWYRACERYEAVIADCVALIALAARTTGIGGFTLRDRSC
DPQAEAELLTVAAAFERFAAIDLLATIIDGEGDRDALARAAAAKVRVATDDNWSDIFSKVLVEHVEPRLGEGRMTVLCDY
PAPEAALARTKAGDPRVAERFEVYACGVELANGFGELTDAAEQRARFTAAMDEKQRRYGERYPLDEDFLAAVGQMPEASG
VALGFDRLVMLAAGATSIDQVVWTPPAGEAS

Sequences:

>Translated_351_residues
MHKEAGASPWWNRDRYLDRQPFLRARGAVTRAARDWFATADFAEVETAILQLSPGNETHLHAPRTELKGPDGARRTRYLR
TSPEFACKKLLAAGEQRIFEFARVFRDRERGRLHLPEFTMLEWYRACERYEAVIADCVALIALAARTTGIGGFTLRDRSC
DPQAEAELLTVAAAFERFAAIDLLATIIDGEGDRDALARAAAAKVRVATDDNWSDIFSKVLVEHVEPRLGEGRMTVLCDY
PAPEAALARTKAGDPRVAERFEVYACGVELANGFGELTDAAEQRARFTAAMDEKQRRYGERYPLDEDFLAAVGQMPEASG
VALGFDRLVMLAAGATSIDQVVWTPPAGEAS
>Mature_351_residues
MHKEAGASPWWNRDRYLDRQPFLRARGAVTRAARDWFATADFAEVETAILQLSPGNETHLHAPRTELKGPDGARRTRYLR
TSPEFACKKLLAAGEQRIFEFARVFRDRERGRLHLPEFTMLEWYRACERYEAVIADCVALIALAARTTGIGGFTLRDRSC
DPQAEAELLTVAAAFERFAAIDLLATIIDGEGDRDALARAAAAKVRVATDDNWSDIFSKVLVEHVEPRLGEGRMTVLCDY
PAPEAALARTKAGDPRVAERFEVYACGVELANGFGELTDAAEQRARFTAAMDEKQRRYGERYPLDEDFLAAVGQMPEASG
VALGFDRLVMLAAGATSIDQVVWTPPAGEAS

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=352, Percent_Identity=24.1477272727273, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI5031815, Length=352, Percent_Identity=24.1477272727273, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI87082379, Length=328, Percent_Identity=36.890243902439, Blast_Score=177, Evalue=6e-46,
Organism=Escherichia coli, GI1790571, Length=349, Percent_Identity=30.0859598853868, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1789256, Length=349, Percent_Identity=30.3724928366762, Blast_Score=142, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI71994340, Length=354, Percent_Identity=26.8361581920904, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17535927, Length=354, Percent_Identity=26.8361581920904, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17535925, Length=355, Percent_Identity=26.7605633802817, Blast_Score=102, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6320242, Length=360, Percent_Identity=25.5555555555556, Blast_Score=103, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24640851, Length=352, Percent_Identity=24.4318181818182, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24640849, Length=352, Percent_Identity=24.4318181818182, Blast_Score=102, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- InterPro:   IPR018149 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6

Molecular weight: Translated: 38857; Mature: 38857

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKEAGASPWWNRDRYLDRQPFLRARGAVTRAARDWFATADFAEVETAILQLSPGNETHL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHEEECCCCCCEE
HAPRTELKGPDGARRTRYLRTSPEFACKKLLAAGEQRIFEFARVFRDRERGRLHLPEFTM
CCCHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHH
LEWYRACERYEAVIADCVALIALAARTTGIGGFTLRDRSCDPQAEAELLTVAAAFERFAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHH
IDLLATIIDGEGDRDALARAAAAKVRVATDDNWSDIFSKVLVEHVEPRLGEGRMTVLCDY
HHHHHHHHCCCCCHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHCCCCCCCCEEEEECC
PAPEAALARTKAGDPRVAERFEVYACGVELANGFGELTDAAEQRARFTAAMDEKQRRYGE
CCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RYPLDEDFLAAVGQMPEASGVALGFDRLVMLAAGATSIDQVVWTPPAGEAS
CCCCCHHHHHHHCCCCCCCCCEEHHHHHHHHHCCCCCCCCEEECCCCCCCC
>Mature Secondary Structure
MHKEAGASPWWNRDRYLDRQPFLRARGAVTRAARDWFATADFAEVETAILQLSPGNETHL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHEEECCCCCCEE
HAPRTELKGPDGARRTRYLRTSPEFACKKLLAAGEQRIFEFARVFRDRERGRLHLPEFTM
CCCHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHH
LEWYRACERYEAVIADCVALIALAARTTGIGGFTLRDRSCDPQAEAELLTVAAAFERFAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHH
IDLLATIIDGEGDRDALARAAAAKVRVATDDNWSDIFSKVLVEHVEPRLGEGRMTVLCDY
HHHHHHHHCCCCCHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHCCCCCCCCEEEEECC
PAPEAALARTKAGDPRVAERFEVYACGVELANGFGELTDAAEQRARFTAAMDEKQRRYGE
CCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RYPLDEDFLAAVGQMPEASGVALGFDRLVMLAAGATSIDQVVWTPPAGEAS
CCCCCHHHHHHHCCCCCCCCCEEHHHHHHHHHCCCCCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]