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The map label for this gene is mvaB [H]

Identifier: 86750049

GI number: 86750049

Start: 3343227

End: 3344132

Strand: Reverse

Name: mvaB [H]

Synonym: RPB_2932

Alternate gene names: 86750049

Gene position: 3344132-3343227 (Counterclockwise)

Preceding gene: 86750050

Following gene: 86750048

Centisome position: 62.72

GC content: 67.0

Gene sequence:

>906_bases
ATGACCGACACCGTCCGCATCGTCGAAGTCGGCCCACGCGACGGGCTGCAGAACGAGAAGACGCCGATCAGCGTCGCGGA
TCGGATCGCCTTCGTCGAGGGCCTCGTCGCGGCTGGCCTGTCCATCGTCGAGGTCGGTGCGTTCGTGTCGCCGAAGGCGG
TGCCACAGATGCAAGGTTCCGACGAGGTGCTGCGCGCATCGCTGAAACTGCCCGGCGAATTCCACGTGCTGGTGCCCAAC
GAGAAGGGCTACGATGCCGCGCGCGCCGCCGGCGCGCAGGTGGTCGCGGTGTTCGCCTCGGCGTCGGAAGGCTTTTCACG
CGCCAACATCAATTGCTCGGTCGCGGAATCGATCGAGCGGTTCAAGCCGGTGATCGCGCGGGCGCAGGCGGACAATGTGA
AAGTCCGCGGTTATGTGTCCTGCGTGCTCGGCTGTCCCTTTGATGGAAATGTGCCGGTGCAGGCGGTGGTCGATACCGCG
ACCACGCTGTGGGATCTCGGCTGCTACGAAGTCTCGCTCGGCGACACGATCGGGGTCGGCACGCCGAACAAGGTCCGCGA
ATTGCTGCGCGCCTGCGCCGACGCGGTCCCGATGGCCGCGCTGGCGATGCATTTCCACGACACCTACGGCCAGGCCCTTG
CCAATCTCTATGCCGGGCTGGAGGAGGGCGCGCGCGTCATCGACAGCGCCGCCGGCGGCCTTGGCGGCTGTCCTTACGCG
CCGGGTGCCACCGGCAATGTCGCGACGGAGGACGTCGTGTACATGCTGGAAGGCATGGGGATCGCGACCGGCATCGACAT
GGACAAGCTCATCGCCGCGACCAACGGCGTCGCGTCGCTACTCGGCCGCCCGCCGGTCAGTCGGGTGGTGAATGCGTTGA
ATGCCAAGCGGAAGCGGGCGGGTTAG

Upstream 100 bases:

>100_bases
GTTCGCCGGCACGCTGACGGCGATCAAGTGCAAGGTCGGCGACATCGTCCAGGAGGGCGCCGAACTCGCCGAGATCGAGC
CGGACGCGGAGTAGCGCAGC

Downstream 100 bases:

>100_bases
CAAAAGCTTGCGTTCGGCTGCTCTGAGCGAAGCTCACGCCGGTATGTTCGACGCAGCGCGTGCATCGTGTTGCGGTGCAC
CCGGATAATCGCGAGGCATT

Product: pyruvate carboxyltransferase

Products: NA

Alternate protein names: HL; HMG-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase [H]

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MTDTVRIVEVGPRDGLQNEKTPISVADRIAFVEGLVAAGLSIVEVGAFVSPKAVPQMQGSDEVLRASLKLPGEFHVLVPN
EKGYDAARAAGAQVVAVFASASEGFSRANINCSVAESIERFKPVIARAQADNVKVRGYVSCVLGCPFDGNVPVQAVVDTA
TTLWDLGCYEVSLGDTIGVGTPNKVRELLRACADAVPMAALAMHFHDTYGQALANLYAGLEEGARVIDSAAGGLGGCPYA
PGATGNVATEDVVYMLEGMGIATGIDMDKLIAATNGVASLLGRPPVSRVVNALNAKRKRAG

Sequences:

>Translated_301_residues
MTDTVRIVEVGPRDGLQNEKTPISVADRIAFVEGLVAAGLSIVEVGAFVSPKAVPQMQGSDEVLRASLKLPGEFHVLVPN
EKGYDAARAAGAQVVAVFASASEGFSRANINCSVAESIERFKPVIARAQADNVKVRGYVSCVLGCPFDGNVPVQAVVDTA
TTLWDLGCYEVSLGDTIGVGTPNKVRELLRACADAVPMAALAMHFHDTYGQALANLYAGLEEGARVIDSAAGGLGGCPYA
PGATGNVATEDVVYMLEGMGIATGIDMDKLIAATNGVASLLGRPPVSRVVNALNAKRKRAG
>Mature_300_residues
TDTVRIVEVGPRDGLQNEKTPISVADRIAFVEGLVAAGLSIVEVGAFVSPKAVPQMQGSDEVLRASLKLPGEFHVLVPNE
KGYDAARAAGAQVVAVFASASEGFSRANINCSVAESIERFKPVIARAQADNVKVRGYVSCVLGCPFDGNVPVQAVVDTAT
TLWDLGCYEVSLGDTIGVGTPNKVRELLRACADAVPMAALAMHFHDTYGQALANLYAGLEEGARVIDSAAGGLGGCPYAP
GATGNVATEDVVYMLEGMGIATGIDMDKLIAATNGVASLLGRPPVSRVVNALNAKRKRAG

Specific function: Involved in the catabolism of branched amino acids such as leucine (Probable) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMG-CoA lyase family [H]

Homologues:

Organism=Homo sapiens, GI62198232, Length=289, Percent_Identity=53.6332179930796, Blast_Score=326, Evalue=1e-89,
Organism=Homo sapiens, GI109150422, Length=293, Percent_Identity=48.4641638225256, Blast_Score=300, Evalue=9e-82,
Organism=Homo sapiens, GI109150427, Length=293, Percent_Identity=48.4641638225256, Blast_Score=300, Evalue=9e-82,
Organism=Homo sapiens, GI260654708, Length=182, Percent_Identity=46.1538461538462, Blast_Score=161, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI17510563, Length=300, Percent_Identity=44.6666666666667, Blast_Score=261, Evalue=4e-70,
Organism=Drosophila melanogaster, GI24582381, Length=298, Percent_Identity=52.3489932885906, Blast_Score=310, Evalue=7e-85,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR000138
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =4.1.3.4 [H]

Molecular weight: Translated: 31185; Mature: 31054

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTVRIVEVGPRDGLQNEKTPISVADRIAFVEGLVAAGLSIVEVGAFVSPKAVPQMQGS
CCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCC
DEVLRASLKLPGEFHVLVPNEKGYDAARAAGAQVVAVFASASEGFSRANINCSVAESIER
HHHHHHHHCCCCEEEEEECCCCCCHHHHHCCCEEEEEEEHHCCCCCCCCCCHHHHHHHHH
FKPVIARAQADNVKVRGYVSCVLGCPFDGNVPVQAVVDTATTLWDLGCYEVSLGDTIGVG
HHHHHHHHCCCCEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEECCCEECCC
TPNKVRELLRACADAVPMAALAMHFHDTYGQALANLYAGLEEGARVIDSAAGGLGGCPYA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PGATGNVATEDVVYMLEGMGIATGIDMDKLIAATNGVASLLGRPPVSRVVNALNAKRKRA
CCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
G
C
>Mature Secondary Structure 
TDTVRIVEVGPRDGLQNEKTPISVADRIAFVEGLVAAGLSIVEVGAFVSPKAVPQMQGS
CCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCC
DEVLRASLKLPGEFHVLVPNEKGYDAARAAGAQVVAVFASASEGFSRANINCSVAESIER
HHHHHHHHCCCCEEEEEECCCCCCHHHHHCCCEEEEEEEHHCCCCCCCCCCHHHHHHHHH
FKPVIARAQADNVKVRGYVSCVLGCPFDGNVPVQAVVDTATTLWDLGCYEVSLGDTIGVG
HHHHHHHHCCCCEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEECCCEECCC
TPNKVRELLRACADAVPMAALAMHFHDTYGQALANLYAGLEEGARVIDSAAGGLGGCPYA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PGATGNVATEDVVYMLEGMGIATGIDMDKLIAATNGVASLLGRPPVSRVVNALNAKRKRA
CCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2687236; 2656635; 1637819 [H]