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The map label for this gene is dus [H]

Identifier: 86750001

GI number: 86750001

Start: 3284224

End: 3285288

Strand: Reverse

Name: dus [H]

Synonym: RPB_2884

Alternate gene names: 86750001

Gene position: 3285288-3284224 (Counterclockwise)

Preceding gene: 86750004

Following gene: 86750000

Centisome position: 61.62

GC content: 68.08

Gene sequence:

>1065_bases
ATGACGCGCTGGCGACAGAATGAGCAGCTTGTGACCCGACCTACAGTAACAGGTTCGCCGCCTTTGAGAATTGGCAAAAT
TGCGGTGGCCAATCGCGTGCTGCTGGCGCCGATGTCCGGCATTACAGATGCGCCGTTTCGCAAACAGGTCGCAGCTCTCG
GCGCCGGGCTCGTGGTATCCGAGATGACCGCCAGCGAAGATCTTGTGCAGGGGCGCGCGATGTCTGTCCGCCGCTGCGAC
GCCATCGACGACGGGCCGCATGTGGTTCAGCTCGCCGGCTGCGAGCCGCACTGGATGGCGGAAGCCGCCCGGATCGCGGA
AGCCGGCGGCGCCGATATCATCGACATCAATATGGGCTGCCCGGCGCGGCATGTGACCGGCGGCCAGTCCGGATCGGCCT
TGATGCGTGATCTCGATCACGCGCTGACGCTGATCGAGGCAACGATCGGCGCGGTGCGCGTGCCGGTGACGCTGAAGATG
CGGCTCGGCTGGGACGATCGCTCGCGCAACGCGCCGGAACTGGCGCGGCGAGCCGAGGCCGCCGGCGTGCAACTCGTGAC
CGTGCATGGCCGCACCCGCAGTCAGTTCTACAAGGGCGAAGCCGACTGGCGTGCCGTCCGCGCGGTCCGCGACGCCGTCG
GCATTCCGCTGGTCGTCAATGGCGATATCACCTCGTATCAGATGGCGGTCGAGGCGCTGGATCAGTCCGGCGCCGACGCG
GTGATGATCGGCCGCGGGGCGCAGGGGCAGCCGTGGCTGCCGGGCCAGATCGGTCGCCGCCTGCAGACCGGGATCGCCGA
AGCGATGCCGTCGCTCACCGATCAGCTCGCTTATCTGCGCGCGCTGTATGACGGCGTGCTCGGCCTGTATGGCGAGCGCA
TCGGGCTGCGCCATGCGCGCAAACATCTCGGCTGGTCGCTCGACGTCGCCGCCGCCGCCAGCGGCGCGCCGCCGGCGACG
CTGAAGCGGTGGAGGACGACGATCCTCACCGACGACAGCCCGACCCGTGTGCATCATGCGCTCACTGACGCCTACGACGA
TTTCGCCTGGAGAGCCGCCGCATGA

Upstream 100 bases:

>100_bases
AGGCGGGAGAATTGCTGCAACGCACTTGCATGTCTGGTATAACTGGCTAAACTGTAGGCAATGACCTATAATGCTCAAAC
ATTGAGCTAGACCGGTCGCA

Downstream 100 bases:

>100_bases
ACGCCGTCCTCGAACCGCGCCACGCCAAGCCGACCGAAAGCGATGCGATCCTCAACGCGCTGCCGAATCCCGTGCTGTTG
ATCGGCCCGGACGGCAAGAT

Product: dihydrouridine synthase TIM-barrel protein NifR3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MTRWRQNEQLVTRPTVTGSPPLRIGKIAVANRVLLAPMSGITDAPFRKQVAALGAGLVVSEMTASEDLVQGRAMSVRRCD
AIDDGPHVVQLAGCEPHWMAEAARIAEAGGADIIDINMGCPARHVTGGQSGSALMRDLDHALTLIEATIGAVRVPVTLKM
RLGWDDRSRNAPELARRAEAAGVQLVTVHGRTRSQFYKGEADWRAVRAVRDAVGIPLVVNGDITSYQMAVEALDQSGADA
VMIGRGAQGQPWLPGQIGRRLQTGIAEAMPSLTDQLAYLRALYDGVLGLYGERIGLRHARKHLGWSLDVAAAASGAPPAT
LKRWRTTILTDDSPTRVHHALTDAYDDFAWRAAA

Sequences:

>Translated_354_residues
MTRWRQNEQLVTRPTVTGSPPLRIGKIAVANRVLLAPMSGITDAPFRKQVAALGAGLVVSEMTASEDLVQGRAMSVRRCD
AIDDGPHVVQLAGCEPHWMAEAARIAEAGGADIIDINMGCPARHVTGGQSGSALMRDLDHALTLIEATIGAVRVPVTLKM
RLGWDDRSRNAPELARRAEAAGVQLVTVHGRTRSQFYKGEADWRAVRAVRDAVGIPLVVNGDITSYQMAVEALDQSGADA
VMIGRGAQGQPWLPGQIGRRLQTGIAEAMPSLTDQLAYLRALYDGVLGLYGERIGLRHARKHLGWSLDVAAAASGAPPAT
LKRWRTTILTDDSPTRVHHALTDAYDDFAWRAAA
>Mature_353_residues
TRWRQNEQLVTRPTVTGSPPLRIGKIAVANRVLLAPMSGITDAPFRKQVAALGAGLVVSEMTASEDLVQGRAMSVRRCDA
IDDGPHVVQLAGCEPHWMAEAARIAEAGGADIIDINMGCPARHVTGGQSGSALMRDLDHALTLIEATIGAVRVPVTLKMR
LGWDDRSRNAPELARRAEAAGVQLVTVHGRTRSQFYKGEADWRAVRAVRDAVGIPLVVNGDITSYQMAVEALDQSGADAV
MIGRGAQGQPWLPGQIGRRLQTGIAEAMPSLTDQLAYLRALYDGVLGLYGERIGLRHARKHLGWSLDVAAAASGAPPATL
KRWRTTILTDDSPTRVHHALTDAYDDFAWRAAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788462, Length=244, Percent_Identity=32.7868852459016, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI239788483, Length=242, Percent_Identity=33.0578512396694, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI31742496, Length=222, Percent_Identity=29.7297297297297, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI40807366, Length=235, Percent_Identity=31.9148936170213, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI8923374, Length=256, Percent_Identity=26.5625, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1789660, Length=335, Percent_Identity=43.2835820895522, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI1788462, Length=231, Percent_Identity=35.4978354978355, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI145693211, Length=287, Percent_Identity=25.7839721254355, Blast_Score=83, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17543114, Length=297, Percent_Identity=30.6397306397306, Blast_Score=111, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI25144369, Length=215, Percent_Identity=31.6279069767442, Blast_Score=101, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17507177, Length=242, Percent_Identity=31.404958677686, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17510279, Length=250, Percent_Identity=26.8, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=232, Percent_Identity=30.6034482758621, Blast_Score=96, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6323433, Length=259, Percent_Identity=25.4826254826255, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24585320, Length=241, Percent_Identity=33.195020746888, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24580595, Length=226, Percent_Identity=32.7433628318584, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI19920448, Length=226, Percent_Identity=32.7433628318584, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI19921524, Length=227, Percent_Identity=31.7180616740088, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI45549423, Length=247, Percent_Identity=29.9595141700405, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 38036; Mature: 37905

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRWRQNEQLVTRPTVTGSPPLRIGKIAVANRVLLAPMSGITDAPFRKQVAALGAGLVVS
CCCCCCCCCEEECCCCCCCCCCEECHHHHHCEEEEECCCCCCCCHHHHHHHHHCCCHHHH
EMTASEDLVQGRAMSVRRCDAIDDGPHVVQLAGCEPHWMAEAARIAEAGGADIIDINMGC
HHHHHHHHHHCHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEECCCC
PARHVTGGQSGSALMRDLDHALTLIEATIGAVRVPVTLKMRLGWDDRSRNAPELARRAEA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCEEEEEEECCCCCCCCCHHHHHHHHH
AGVQLVTVHGRTRSQFYKGEADWRAVRAVRDAVGIPLVVNGDITSYQMAVEALDQSGADA
CCEEEEEEECCHHHHHHCCCCHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHCCCCCE
VMIGRGAQGQPWLPGQIGRRLQTGIAEAMPSLTDQLAYLRALYDGVLGLYGERIGLRHAR
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KHLGWSLDVAAAASGAPPATLKRWRTTILTDDSPTRVHHALTDAYDDFAWRAAA
HHCCCCEEHHHHCCCCCHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TRWRQNEQLVTRPTVTGSPPLRIGKIAVANRVLLAPMSGITDAPFRKQVAALGAGLVVS
CCCCCCCCEEECCCCCCCCCCEECHHHHHCEEEEECCCCCCCCHHHHHHHHHCCCHHHH
EMTASEDLVQGRAMSVRRCDAIDDGPHVVQLAGCEPHWMAEAARIAEAGGADIIDINMGC
HHHHHHHHHHCHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEECCCC
PARHVTGGQSGSALMRDLDHALTLIEATIGAVRVPVTLKMRLGWDDRSRNAPELARRAEA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCEEEEEEECCCCCCCCCHHHHHHHHH
AGVQLVTVHGRTRSQFYKGEADWRAVRAVRDAVGIPLVVNGDITSYQMAVEALDQSGADA
CCEEEEEEECCHHHHHHCCCCHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHCCCCCE
VMIGRGAQGQPWLPGQIGRRLQTGIAEAMPSLTDQLAYLRALYDGVLGLYGERIGLRHAR
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KHLGWSLDVAAAASGAPPATLKRWRTTILTDDSPTRVHHALTDAYDDFAWRAAA
HHCCCCEEHHHHCCCCCHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8412703 [H]