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The map label for this gene is pdhC [H]

Identifier: 86749885

GI number: 86749885

Start: 3156118

End: 3157473

Strand: Reverse

Name: pdhC [H]

Synonym: RPB_2768

Alternate gene names: 86749885

Gene position: 3157473-3156118 (Counterclockwise)

Preceding gene: 86749886

Following gene: 86749884

Centisome position: 59.22

GC content: 66.3

Gene sequence:

>1356_bases
ATGCCGATCAACATTCTGATGCCCGCGCTGTCCCCGACCATGGAGAAGGGCAACCTTGCGAAGTGGCTGAAGAAGGAGGG
CGACAAGGTCAAGAGCGGCGATGTCATCGCCGAAATCGAGACCGACAAGGCGACGATGGAAGTCGAGGCCGCCGACGAGG
GCACGCTGGCGAAGATCCTGGTGCCGGAAGGCACCCAGGACGTTGCGGTCAACGCCGTGATCGCGGTGCTGGCCGGCGAC
GGCGAGGACGTTGCGACGGCCGGGGCAGGCGCTGGGAAGGCGGAAGCGCCGAAGGCAGAGGCCCCCAAAGCCGAGGCCCC
CAAAGCGAATCTCGCGGAAGCCAAACCCGAGAAGGCCACCACGCCAGCCGCCAAGGACGGGGCGCCGCGCGCTGCTTCGC
CCGAGGCCGCTCACACCAACGGCGCGCGCGTCTTCTCGTCGCCGCTGGCGCGACGGCTCGCCAAGGATTCCGGGATCGAT
CTGGCCCGCGTCGAAGGCAGCGGGCCGCACGGCCGCGTCATCGCCCGCGACATTGAAAAAGCCAAGGCCGGCGGCGGCCT
CAAGGCGCCGGCAGCGGCTCCGGCTTCGTCCGCAGCGCCCAGCGTCGCGCCGTCGATGTCCGACCAGCAGATCCGCGCGC
TATATCCGGAAGGCAGCTACGAGGTGGTGGCGCATGACGGCATGCGCCGGACGATTGCGCAGCGGCTGACGCAATCGACC
CAGACCATCCCGCATTTCTATCTGACGATCGACTGCAATCTCGACCGGCTGATGGCCGCGCGCGAGGACATCAACGCCGC
CGCCCCGAAAGACAAGGACGGCAAGCCGGCCTACAAGCTGTCGGTCAACGATTTCGTCATCAAGGCGATGGCGATCGCGC
TGCAGCGTATCCCGGACGCCAACGTGTCGTGGACGGAAGCCGGAATGCTCAAGCACAAGCATTCCGACATTGGCGTCGCC
GTCGCGATGCCGGGCGGACTGATCACCCCGATCATCCGCAGCGCCGAGACGGCGTCGCTGTCATATATCTCCGCGCAGAT
GAAGGATTTCGCCGCACGTGCTCGGGCGCGCAAGCTGAAGCCCGAAGAGTATCAGGGCGGCACCACCGCGGTGTCCAACC
TCGGCATGTACGGCATCAAGGACTTCACGGCAGTGATCAACCCGCCGCACGCCACCATCCTCGCCGTCGGCACCGGCGAG
CAGCGCCCGATCGTTTGTAACGGCCAGATCGAGATCGCCACGATGATGAGCGTGACGCTGAGCTGCGATCACCGCGCCGT
CGACGGCGCGCTCGGCGCCGAACTGATCGGCGCGTTCAAGACGCTGATCGAAAATCCCGTGATGATGATGGTGTGA

Upstream 100 bases:

>100_bases
CGAGGCGCTGGGCCGCATCGTCGAGATGTTCGAGGCCGAGATCGCGCGCCCGTCGGATCTGGGCAGCACCACCGAACGGC
CGCAGTAAGGTATTCACGCC

Downstream 100 bases:

>100_bases
CATGCCGCTAGTCCGGATTTCGCTGCGCGCCGGCAAAAGCGAGGCCTACCGGCAGGCGGTCGGCGAGGGCGTGTATCAGG
CGCTGCGCGACACCTTCAAC

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MPINILMPALSPTMEKGNLAKWLKKEGDKVKSGDVIAEIETDKATMEVEAADEGTLAKILVPEGTQDVAVNAVIAVLAGD
GEDVATAGAGAGKAEAPKAEAPKAEAPKANLAEAKPEKATTPAAKDGAPRAASPEAAHTNGARVFSSPLARRLAKDSGID
LARVEGSGPHGRVIARDIEKAKAGGGLKAPAAAPASSAAPSVAPSMSDQQIRALYPEGSYEVVAHDGMRRTIAQRLTQST
QTIPHFYLTIDCNLDRLMAAREDINAAAPKDKDGKPAYKLSVNDFVIKAMAIALQRIPDANVSWTEAGMLKHKHSDIGVA
VAMPGGLITPIIRSAETASLSYISAQMKDFAARARARKLKPEEYQGGTTAVSNLGMYGIKDFTAVINPPHATILAVGTGE
QRPIVCNGQIEIATMMSVTLSCDHRAVDGALGAELIGAFKTLIENPVMMMV

Sequences:

>Translated_451_residues
MPINILMPALSPTMEKGNLAKWLKKEGDKVKSGDVIAEIETDKATMEVEAADEGTLAKILVPEGTQDVAVNAVIAVLAGD
GEDVATAGAGAGKAEAPKAEAPKAEAPKANLAEAKPEKATTPAAKDGAPRAASPEAAHTNGARVFSSPLARRLAKDSGID
LARVEGSGPHGRVIARDIEKAKAGGGLKAPAAAPASSAAPSVAPSMSDQQIRALYPEGSYEVVAHDGMRRTIAQRLTQST
QTIPHFYLTIDCNLDRLMAAREDINAAAPKDKDGKPAYKLSVNDFVIKAMAIALQRIPDANVSWTEAGMLKHKHSDIGVA
VAMPGGLITPIIRSAETASLSYISAQMKDFAARARARKLKPEEYQGGTTAVSNLGMYGIKDFTAVINPPHATILAVGTGE
QRPIVCNGQIEIATMMSVTLSCDHRAVDGALGAELIGAFKTLIENPVMMMV
>Mature_450_residues
PINILMPALSPTMEKGNLAKWLKKEGDKVKSGDVIAEIETDKATMEVEAADEGTLAKILVPEGTQDVAVNAVIAVLAGDG
EDVATAGAGAGKAEAPKAEAPKAEAPKANLAEAKPEKATTPAAKDGAPRAASPEAAHTNGARVFSSPLARRLAKDSGIDL
ARVEGSGPHGRVIARDIEKAKAGGGLKAPAAAPASSAAPSVAPSMSDQQIRALYPEGSYEVVAHDGMRRTIAQRLTQSTQ
TIPHFYLTIDCNLDRLMAAREDINAAAPKDKDGKPAYKLSVNDFVIKAMAIALQRIPDANVSWTEAGMLKHKHSDIGVAV
AMPGGLITPIIRSAETASLSYISAQMKDFAARARARKLKPEEYQGGTTAVSNLGMYGIKDFTAVINPPHATILAVGTGEQ
RPIVCNGQIEIATMMSVTLSCDHRAVDGALGAELIGAFKTLIENPVMMMV

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=453, Percent_Identity=41.0596026490066, Blast_Score=332, Evalue=5e-91,
Organism=Homo sapiens, GI203098816, Length=477, Percent_Identity=37.1069182389937, Blast_Score=281, Evalue=1e-75,
Organism=Homo sapiens, GI203098753, Length=476, Percent_Identity=36.9747899159664, Blast_Score=280, Evalue=2e-75,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=43.4782608695652, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI110671329, Length=440, Percent_Identity=25.4545454545455, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI19923748, Length=263, Percent_Identity=30.4182509505703, Blast_Score=116, Evalue=4e-26,
Organism=Escherichia coli, GI1786946, Length=449, Percent_Identity=30.0668151447661, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1786305, Length=308, Percent_Identity=31.1688311688312, Blast_Score=140, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17560088, Length=459, Percent_Identity=43.1372549019608, Blast_Score=331, Evalue=4e-91,
Organism=Caenorhabditis elegans, GI17538894, Length=322, Percent_Identity=32.9192546583851, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI25146366, Length=451, Percent_Identity=29.0465631929047, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17537937, Length=444, Percent_Identity=30.1801801801802, Blast_Score=149, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6324258, Length=458, Percent_Identity=40.8296943231441, Blast_Score=315, Evalue=8e-87,
Organism=Saccharomyces cerevisiae, GI6320352, Length=229, Percent_Identity=32.3144104803493, Blast_Score=119, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6321632, Length=115, Percent_Identity=45.2173913043478, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI20129315, Length=456, Percent_Identity=40.1315789473684, Blast_Score=272, Evalue=4e-73,
Organism=Drosophila melanogaster, GI24582497, Length=445, Percent_Identity=39.5505617977528, Blast_Score=257, Evalue=1e-68,
Organism=Drosophila melanogaster, GI18859875, Length=442, Percent_Identity=28.2805429864253, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24645909, Length=239, Percent_Identity=30.9623430962343, Blast_Score=106, Evalue=4e-23,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 47090; Mature: 46959

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPINILMPALSPTMEKGNLAKWLKKEGDKVKSGDVIAEIETDKATMEVEAADEGTLAKIL
CCCEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCEEEEEEECCCCCEEEEE
VPEGTQDVAVNAVIAVLAGDGEDVATAGAGAGKAEAPKAEAPKAEAPKANLAEAKPEKAT
ECCCCCHHEEEEEEEEEECCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TPAAKDGAPRAASPEAAHTNGARVFSSPLARRLAKDSGIDLARVEGSGPHGRVIARDIEK
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHH
AKAGGGLKAPAAAPASSAAPSVAPSMSDQQIRALYPEGSYEVVAHDGMRRTIAQRLTQST
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCEEEEECCCHHHHHHHHHHHHH
QTIPHFYLTIDCNLDRLMAAREDINAAAPKDKDGKPAYKLSVNDFVIKAMAIALQRIPDA
CCCCEEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCC
NVSWTEAGMLKHKHSDIGVAVAMPGGLITPIIRSAETASLSYISAQMKDFAARARARKLK
CCCHHHHHHHHCCCCCCCEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
PEEYQGGTTAVSNLGMYGIKDFTAVINPPHATILAVGTGEQRPIVCNGQIEIATMMSVTL
CHHCCCCHHHHHHCCCCCCCHHHEEECCCCEEEEEEECCCCCCEEECCCEEEEEEEEEEE
SCDHRAVDGALGAELIGAFKTLIENPVMMMV
ECCCHHHHHHHHHHHHHHHHHHHCCCEEEEC
>Mature Secondary Structure 
PINILMPALSPTMEKGNLAKWLKKEGDKVKSGDVIAEIETDKATMEVEAADEGTLAKIL
CCEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCEEEEEEECCCCCEEEEE
VPEGTQDVAVNAVIAVLAGDGEDVATAGAGAGKAEAPKAEAPKAEAPKANLAEAKPEKAT
ECCCCCHHEEEEEEEEEECCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TPAAKDGAPRAASPEAAHTNGARVFSSPLARRLAKDSGIDLARVEGSGPHGRVIARDIEK
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHH
AKAGGGLKAPAAAPASSAAPSVAPSMSDQQIRALYPEGSYEVVAHDGMRRTIAQRLTQST
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCEEEEECCCHHHHHHHHHHHHH
QTIPHFYLTIDCNLDRLMAAREDINAAAPKDKDGKPAYKLSVNDFVIKAMAIALQRIPDA
CCCCEEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCC
NVSWTEAGMLKHKHSDIGVAVAMPGGLITPIIRSAETASLSYISAQMKDFAARARARKLK
CCCHHHHHHHHCCCCCCCEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
PEEYQGGTTAVSNLGMYGIKDFTAVINPPHATILAVGTGEQRPIVCNGQIEIATMMSVTL
CHHCCCCHHHHHHCCCCCCCHHHEEECCCCEEEEEEECCCCCCEEECCCEEEEEEEEEEE
SCDHRAVDGALGAELIGAFKTLIENPVMMMV
ECCCHHHHHHHHHHHHHHHHHHHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]