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The map label for this gene is glyA [H]

Identifier: 86749751

GI number: 86749751

Start: 3014562

End: 3015863

Strand: Direct

Name: glyA [H]

Synonym: RPB_2634

Alternate gene names: 86749751

Gene position: 3014562-3015863 (Clockwise)

Preceding gene: 86749749

Following gene: 86749752

Centisome position: 56.54

GC content: 65.75

Gene sequence:

>1302_bases
ATGAGCACATCAGCCAAGCACACGTCCGCGCCCGACAGCTTCTTCTCCGCCTCGCTCGAGCAGGCCGATCCCGAGATCGC
CGCTGCGATCAAGGGTGAGCTCGGCCGCCAGCGCCACGAGGTCGAGCTGATCGCCTCCGAGAACATCGTCAGCCGCGCCG
TGCTGGAAGCGCAGGGCTCGGTGATGACCAACAAATATGCCGAGGGCTATCCGGGCGCGCGCTATTACGGCGGCTGCGAA
TTCGTCGACGTCGCCGAGAATCTGGCGATCGAGCGCGCCAAGAAGCTGTTCGGCGCTGGTTTTGCCAACGTCCAGCCGAA
CTCGGGCAGCCAGATGAACCAGGCGGTGTTCCTGGCGCTGCTGCAGCCCGGCGACACCTTCATGGGGCTCGATCTCGCCG
CCGGCGGCCATCTGACCCACGGCGCGACCGTCAATATGAGCGGCAAGTGGTTCAAGCCGGTGCACTACACCGTGCGCCGC
GAGGACGGCATCATCGACATGGATGCGGTCGCCAAGCTGGCCGAGGAGACCCGGCCGAAGCTGATCATCGCCGGCGGCTC
GGCCTATTCGCGGGCCTGGGACTTCAAGCGCTTCCGTGAGATCGCCGACAGCGTCGGGGCCTATTTCATGGTCGACATGG
CGCATTTCGCCGGCCTGGTGGCGGGCGGAGCGCATGCCTCGCCGGTGCCGCACGCCCATGTCTGCACCACCACCACCCAC
AAATCGCTGCGCGGCCCGCGCGGCGGCCTGATCCTCACCAACGACGAGGCGCTGGCCAAGAAGTTCAACTCGGCGATCTT
TCCCGGTCTACAGGGCGGCCCGCTGATGCATGTGATCGCCGCCAAGGCGGTGGCGTTCAAGGAAGCGCTCCAGCCCGACT
TCAAGGTCTACGCGAAGAACGTCGTCGAAAATGCAAAGGCGCTTGCGGAAACGCTGCGTGCGGCAGGCTTCGATCTGGTC
TCCGGCGGCACCGACAACCATCTCATGCTGGTCGACCTGCGGCCGAAGGGGCTGAAGGGCAACGTCTCCGAGAAGGCGCT
GGTCCGCGCCGCCATCACCTGCAACAAGAACGGGATCCCGTTCGACCCGGAAAAGCCGTTCGTGACCTCCGGCCTGCGGC
TCGGCACTCCGGCGGCGACGACCCGCGGATTCGGCGTCGCCGAGTTCCAGCAGGTTGGGAACCTGATCGCCGAAGTGCTG
AACGCCATTGCGCAATCCCCCGACGGTGCCGCGCCGCTGGTGGAGGCCTCGGTGAAGCAGCGGGTCAAGGAATTGACCGA
CCGCTTTCCGATCTATCAGTAA

Upstream 100 bases:

>100_bases
GGCGGGTCCGGATATGCTATGGCGCAGCCGCCGCTTCCGAACCGCACCCGATGACCGTTCGCACCCGTGACACGCGGCTG
CGGCGGTGGAGACATTGCCC

Downstream 100 bases:

>100_bases
CTGACCACCGTCATGCCCGGGCTTGACCCGGGCATCCACGTCTTTACGGCGTGGCAGCGGGAGCAAGGCGTGGATGGCCG
GGACGAGCCCGGCCATGACG

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MSTSAKHTSAPDSFFSASLEQADPEIAAAIKGELGRQRHEVELIASENIVSRAVLEAQGSVMTNKYAEGYPGARYYGGCE
FVDVAENLAIERAKKLFGAGFANVQPNSGSQMNQAVFLALLQPGDTFMGLDLAAGGHLTHGATVNMSGKWFKPVHYTVRR
EDGIIDMDAVAKLAEETRPKLIIAGGSAYSRAWDFKRFREIADSVGAYFMVDMAHFAGLVAGGAHASPVPHAHVCTTTTH
KSLRGPRGGLILTNDEALAKKFNSAIFPGLQGGPLMHVIAAKAVAFKEALQPDFKVYAKNVVENAKALAETLRAAGFDLV
SGGTDNHLMLVDLRPKGLKGNVSEKALVRAAITCNKNGIPFDPEKPFVTSGLRLGTPAATTRGFGVAEFQQVGNLIAEVL
NAIAQSPDGAAPLVEASVKQRVKELTDRFPIYQ

Sequences:

>Translated_433_residues
MSTSAKHTSAPDSFFSASLEQADPEIAAAIKGELGRQRHEVELIASENIVSRAVLEAQGSVMTNKYAEGYPGARYYGGCE
FVDVAENLAIERAKKLFGAGFANVQPNSGSQMNQAVFLALLQPGDTFMGLDLAAGGHLTHGATVNMSGKWFKPVHYTVRR
EDGIIDMDAVAKLAEETRPKLIIAGGSAYSRAWDFKRFREIADSVGAYFMVDMAHFAGLVAGGAHASPVPHAHVCTTTTH
KSLRGPRGGLILTNDEALAKKFNSAIFPGLQGGPLMHVIAAKAVAFKEALQPDFKVYAKNVVENAKALAETLRAAGFDLV
SGGTDNHLMLVDLRPKGLKGNVSEKALVRAAITCNKNGIPFDPEKPFVTSGLRLGTPAATTRGFGVAEFQQVGNLIAEVL
NAIAQSPDGAAPLVEASVKQRVKELTDRFPIYQ
>Mature_432_residues
STSAKHTSAPDSFFSASLEQADPEIAAAIKGELGRQRHEVELIASENIVSRAVLEAQGSVMTNKYAEGYPGARYYGGCEF
VDVAENLAIERAKKLFGAGFANVQPNSGSQMNQAVFLALLQPGDTFMGLDLAAGGHLTHGATVNMSGKWFKPVHYTVRRE
DGIIDMDAVAKLAEETRPKLIIAGGSAYSRAWDFKRFREIADSVGAYFMVDMAHFAGLVAGGAHASPVPHAHVCTTTTHK
SLRGPRGGLILTNDEALAKKFNSAIFPGLQGGPLMHVIAAKAVAFKEALQPDFKVYAKNVVENAKALAETLRAAGFDLVS
GGTDNHLMLVDLRPKGLKGNVSEKALVRAAITCNKNGIPFDPEKPFVTSGLRLGTPAATTRGFGVAEFQQVGNLIAEVLN
AIAQSPDGAAPLVEASVKQRVKELTDRFPIYQ

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI261862352, Length=452, Percent_Identity=45.353982300885, Blast_Score=352, Evalue=4e-97,
Organism=Homo sapiens, GI261862350, Length=452, Percent_Identity=45.353982300885, Blast_Score=352, Evalue=4e-97,
Organism=Homo sapiens, GI261862348, Length=452, Percent_Identity=45.353982300885, Blast_Score=352, Evalue=4e-97,
Organism=Homo sapiens, GI19923315, Length=452, Percent_Identity=45.353982300885, Blast_Score=352, Evalue=5e-97,
Organism=Homo sapiens, GI22547186, Length=447, Percent_Identity=46.0850111856823, Blast_Score=339, Evalue=3e-93,
Organism=Homo sapiens, GI261862346, Length=452, Percent_Identity=44.6902654867257, Blast_Score=338, Evalue=6e-93,
Organism=Homo sapiens, GI22547189, Length=430, Percent_Identity=43.7209302325581, Blast_Score=304, Evalue=1e-82,
Organism=Escherichia coli, GI1788902, Length=412, Percent_Identity=57.0388349514563, Blast_Score=442, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI25144732, Length=402, Percent_Identity=50, Blast_Score=363, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI25144729, Length=402, Percent_Identity=50, Blast_Score=362, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6319739, Length=441, Percent_Identity=43.9909297052154, Blast_Score=354, Evalue=2e-98,
Organism=Saccharomyces cerevisiae, GI6323087, Length=447, Percent_Identity=44.5190156599553, Blast_Score=338, Evalue=8e-94,
Organism=Drosophila melanogaster, GI24640005, Length=419, Percent_Identity=46.7780429594272, Blast_Score=357, Evalue=9e-99,
Organism=Drosophila melanogaster, GI221329721, Length=419, Percent_Identity=46.7780429594272, Blast_Score=356, Evalue=1e-98,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 46134; Mature: 46003

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSAKHTSAPDSFFSASLEQADPEIAAAIKGELGRQRHEVELIASENIVSRAVLEAQGS
CCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCHHHEEEEECCHHHHHHHHHHCCC
VMTNKYAEGYPGARYYGGCEFVDVAENLAIERAKKLFGAGFANVQPNSGSQMNQAVFLAL
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHEEEEEE
LQPGDTFMGLDLAAGGHLTHGATVNMSGKWFKPVHYTVRREDGIIDMDAVAKLAEETRPK
ECCCCCEEEEEEECCCCCCCCEEECCCCCEECCEEEEEECCCCCCCHHHHHHHHHHCCCE
LIIAGGSAYSRAWDFKRFREIADSVGAYFMVDMAHFAGLVAGGAHASPVPHAHVCTTTTH
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCH
KSLRGPRGGLILTNDEALAKKFNSAIFPGLQGGPLMHVIAAKAVAFKEALQPDFKVYAKN
HHHCCCCCCEEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
VVENAKALAETLRAAGFDLVSGGTDNHLMLVDLRPKGLKGNVSEKALVRAAITCNKNGIP
HHHHHHHHHHHHHHCCCEEECCCCCCEEEEEEECCCCCCCCCCHHHHHHHHEEECCCCCC
FDPEKPFVTSGLRLGTPAATTRGFGVAEFQQVGNLIAEVLNAIAQSPDGAAPLVEASVKQ
CCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
RVKELTDRFPIYQ
HHHHHHHHCCCCC
>Mature Secondary Structure 
STSAKHTSAPDSFFSASLEQADPEIAAAIKGELGRQRHEVELIASENIVSRAVLEAQGS
CCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCHHHEEEEECCHHHHHHHHHHCCC
VMTNKYAEGYPGARYYGGCEFVDVAENLAIERAKKLFGAGFANVQPNSGSQMNQAVFLAL
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHEEEEEE
LQPGDTFMGLDLAAGGHLTHGATVNMSGKWFKPVHYTVRREDGIIDMDAVAKLAEETRPK
ECCCCCEEEEEEECCCCCCCCEEECCCCCEECCEEEEEECCCCCCCHHHHHHHHHHCCCE
LIIAGGSAYSRAWDFKRFREIADSVGAYFMVDMAHFAGLVAGGAHASPVPHAHVCTTTTH
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCH
KSLRGPRGGLILTNDEALAKKFNSAIFPGLQGGPLMHVIAAKAVAFKEALQPDFKVYAKN
HHHCCCCCCEEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
VVENAKALAETLRAAGFDLVSGGTDNHLMLVDLRPKGLKGNVSEKALVRAAITCNKNGIP
HHHHHHHHHHHHHHCCCEEECCCCCCEEEEEEECCCCCCCCCCHHHHHHHHEEECCCCCC
FDPEKPFVTSGLRLGTPAATTRGFGVAEFQQVGNLIAEVLNAIAQSPDGAAPLVEASVKQ
CCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
RVKELTDRFPIYQ
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA