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The map label for this gene is hemB [H]

Identifier: 86749740

GI number: 86749740

Start: 3005621

End: 3006673

Strand: Reverse

Name: hemB [H]

Synonym: RPB_2623

Alternate gene names: 86749740

Gene position: 3006673-3005621 (Counterclockwise)

Preceding gene: 86749750

Following gene: 86749739

Centisome position: 56.39

GC content: 68.0

Gene sequence:

>1053_bases
ATGGCGATCAAATTCGGCCGTCCCATCGAAATGCGCGACCCCGCCCCCCGGCCGTCCGCCCCGTGCCTCGACCTCACCGT
GCGCCCGCGTCGCAACCGCAAGTCCGAATGGGCTCGCCGGCTGGTGCGGGAGAATTGCGTCACCACGGACGACCTGATCT
GGCCGCTGTTCGTGGTCGAAGGCAGCAATCAGCGGGTCGAGATCGCCTCGATGCCGGGCGTCGAGCGGCTCAGCGTCGAT
CAGGTGGTTCGCGCCGCCGAGCGCGCCGCCAAACTCGACATCCCCTGCATTGCGCTGTTCCCCTACACGGACCCGTCGCT
GCGCGACGAGCACGGCACGGAAGCGACCAACGCCGAGAACCTGGTGTGCCGCACGGTGCGCGCCATCAAGAAGGAGTTCC
CCGAGATCGGCGTGCTGTGCGACGTGGCGCTCGATCCGTTCACCAGCCATGGCCATGATGGCCTGATCCAGGACGGCCGC
ATCCTCAACGACGAGACCGTCGCGGTGCTGGTCCAACAGGCGCTGGTGCAGGCCCAGGCCGGCTGCGACGTGATCGCGCC
GTCCGACATGATGGACGGCCGCATCGGCGCCATCCGCGCAGGCCTCGACGACGCCGGCTTCCAGGACGTCCAGATCATGG
CCTATGCGGCGAAATACGCCTCGGCGTTCTACGGCCCGTTCCGCGACGCGATCGGCTCGGCCAAGGCGCTGACCGGCGAC
AAGCGCACCTACCAGATGGACCCGGCCAACACCGACGAGGCGATCCGCGAGGTCGAACTCGACCTCGCCGAGGGCGCCGA
TATGGTCATGGTGAAGCCCGGCATGCCCTATCTCGACATCGTCCGCCGGGTGAAGGAGACCTTCGCGGTGCCGACCTTCG
CCTATCAGGTGTCGGGCGAATACGCGATGATCGCCGCGGCCGCGAACAACGGCTGGCTCGACGGCGAGCGGGCGATGATG
GAGAGCCTGCTCGGCTTCAAGCGCGCCGGCGCCGACGGCGTACTGACCTACTTCGCCCCGCAGGCCGCCGAGAAGCTGAA
ACAGCAAGGCTGA

Upstream 100 bases:

>100_bases
CCGACGCCGCCGATTGCGCCAAATCAACAGCGCAGTGACGCGGCAATGCCAAAATGCTAGAATTGCACCAAACCGGACGC
CCACAGGGAGAGTTGGCACC

Downstream 100 bases:

>100_bases
TATCAGGGCCGACCGCTCCCGTACCACGCGAAGGGTTGCCGCTCTGTAATGTCGCGGCGGCCTTGCAACGCATGCCGACC
GTCGCCATTTCCAGCGCGTC

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MAIKFGRPIEMRDPAPRPSAPCLDLTVRPRRNRKSEWARRLVRENCVTTDDLIWPLFVVEGSNQRVEIASMPGVERLSVD
QVVRAAERAAKLDIPCIALFPYTDPSLRDEHGTEATNAENLVCRTVRAIKKEFPEIGVLCDVALDPFTSHGHDGLIQDGR
ILNDETVAVLVQQALVQAQAGCDVIAPSDMMDGRIGAIRAGLDDAGFQDVQIMAYAAKYASAFYGPFRDAIGSAKALTGD
KRTYQMDPANTDEAIREVELDLAEGADMVMVKPGMPYLDIVRRVKETFAVPTFAYQVSGEYAMIAAAANNGWLDGERAMM
ESLLGFKRAGADGVLTYFAPQAAEKLKQQG

Sequences:

>Translated_350_residues
MAIKFGRPIEMRDPAPRPSAPCLDLTVRPRRNRKSEWARRLVRENCVTTDDLIWPLFVVEGSNQRVEIASMPGVERLSVD
QVVRAAERAAKLDIPCIALFPYTDPSLRDEHGTEATNAENLVCRTVRAIKKEFPEIGVLCDVALDPFTSHGHDGLIQDGR
ILNDETVAVLVQQALVQAQAGCDVIAPSDMMDGRIGAIRAGLDDAGFQDVQIMAYAAKYASAFYGPFRDAIGSAKALTGD
KRTYQMDPANTDEAIREVELDLAEGADMVMVKPGMPYLDIVRRVKETFAVPTFAYQVSGEYAMIAAAANNGWLDGERAMM
ESLLGFKRAGADGVLTYFAPQAAEKLKQQG
>Mature_349_residues
AIKFGRPIEMRDPAPRPSAPCLDLTVRPRRNRKSEWARRLVRENCVTTDDLIWPLFVVEGSNQRVEIASMPGVERLSVDQ
VVRAAERAAKLDIPCIALFPYTDPSLRDEHGTEATNAENLVCRTVRAIKKEFPEIGVLCDVALDPFTSHGHDGLIQDGRI
LNDETVAVLVQQALVQAQAGCDVIAPSDMMDGRIGAIRAGLDDAGFQDVQIMAYAAKYASAFYGPFRDAIGSAKALTGDK
RTYQMDPANTDEAIREVELDLAEGADMVMVKPGMPYLDIVRRVKETFAVPTFAYQVSGEYAMIAAAANNGWLDGERAMME
SLLGFKRAGADGVLTYFAPQAAEKLKQQG

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen. Required for nodule development [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=306, Percent_Identity=42.483660130719, Blast_Score=245, Evalue=6e-65,
Organism=Escherichia coli, GI87081728, Length=322, Percent_Identity=47.2049689440994, Blast_Score=266, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6321398, Length=316, Percent_Identity=39.2405063291139, Blast_Score=207, Evalue=2e-54,
Organism=Drosophila melanogaster, GI21358291, Length=304, Percent_Identity=40.1315789473684, Blast_Score=250, Evalue=9e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 38340; Mature: 38209

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIKFGRPIEMRDPAPRPSAPCLDLTVRPRRNRKSEWARRLVRENCVTTDDLIWPLFVVE
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEE
GSNQRVEIASMPGVERLSVDQVVRAAERAAKLDIPCIALFPYTDPSLRDEHGTEATNAEN
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCHHH
LVCRTVRAIKKEFPEIGVLCDVALDPFTSHGHDGLIQDGRILNDETVAVLVQQALVQAQA
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHC
GCDVIAPSDMMDGRIGAIRAGLDDAGFQDVQIMAYAAKYASAFYGPFRDAIGSAKALTGD
CCCEECCCHHCCCCHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCC
KRTYQMDPANTDEAIREVELDLAEGADMVMVKPGMPYLDIVRRVKETFAVPTFAYQVSGE
CCEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCC
YAMIAAAANNGWLDGERAMMESLLGFKRAGADGVLTYFAPQAAEKLKQQG
EEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEHHCHHHHHHHHHCC
>Mature Secondary Structure 
AIKFGRPIEMRDPAPRPSAPCLDLTVRPRRNRKSEWARRLVRENCVTTDDLIWPLFVVE
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEE
GSNQRVEIASMPGVERLSVDQVVRAAERAAKLDIPCIALFPYTDPSLRDEHGTEATNAEN
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCHHH
LVCRTVRAIKKEFPEIGVLCDVALDPFTSHGHDGLIQDGRILNDETVAVLVQQALVQAQA
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHC
GCDVIAPSDMMDGRIGAIRAGLDDAGFQDVQIMAYAAKYASAFYGPFRDAIGSAKALTGD
CCCEECCCHHCCCCHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCC
KRTYQMDPANTDEAIREVELDLAEGADMVMVKPGMPYLDIVRRVKETFAVPTFAYQVSGE
CCEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCC
YAMIAAAANNGWLDGERAMMESLLGFKRAGADGVLTYFAPQAAEKLKQQG
EEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEHHCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226669; 12597275 [H]