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The map label for this gene is glnA [H]

Identifier: 86749676

GI number: 86749676

Start: 2933968

End: 2935377

Strand: Reverse

Name: glnA [H]

Synonym: RPB_2558

Alternate gene names: 86749676

Gene position: 2935377-2933968 (Counterclockwise)

Preceding gene: 86749677

Following gene: 86749675

Centisome position: 55.06

GC content: 61.7

Gene sequence:

>1410_bases
ATGACCACCGCCAAAGAAGTCCTGAAATCCATCAAGGACAACGACGTCAAGTACGTCGATCTTCGTTTCACCGACCCGCG
CGGCAAGTGGCAGCACGTCACTTTCGACGTCAGCATGATCGACGAGGAGATCTTCGCCGAGGGCACGATGTTCGACGGTT
CCTCGATCGCCGGCTGGAAGGCGATCAATGAATCCGACATGATGCTGATGCTCGACCCGACCACCGCGGTGATCGACCCG
TTCTTCGCCGAGACCACCATGGTCATCACCTGCGACATCCTCGAGCCGTCGACCGGTGAGCCCTACAACCGCGACCCGCG
CGGCATCGCCAAGAAGGCCGAGGCCCAGGTCAAGGCGATGGGCGTCGGCGACTCGGTGTTCGTCGGTCCGGAAGCCGAGT
TCTTCGTGTTCGACGACGTGCGCTATTCGGCGAACCCGTACAACACCGGCTTCAAGCTGGATTCGTCGGAACTGCCGACC
AACTCCGACACCGAATACGAGGGCGGCAATCTCGGCCACCGCATCCGCACCAAGGCCGGCTACTTCCCGGTCCCGCCGCA
GGATTCGGTGCAGGACATGCGCTCGGAAATGCTCGGCGCGATGGCGCGGATGGGCGTCAAGGTCGAGAAGCATCACCACG
AAGTCGCTTCCGCCCAGCACGAGCTCGGCATGAAGTTCGACACCCTGACCCACATGGCCGACCAGATGCAGATCTACAAA
TACTGCATCCATCAGGTCGCCCACATCTACGGCAAGACCGCGACCTTCATGCCGAAGCCGGTGTTCGGCGACAACGGCTC
GGGCATGCATGTGCACCAGTCGATCTGGAAGGACGGCAAGCCGGTGTTCGCCGGCAACAAATACGCCGACCTGTCCGAGA
CCTGCCTGCACTACATCGGCGGCATCATCAGCCACGCCAAGGCGATCAACGCCTTCACCAACCCGTCGACCAATTCCTAC
AAGCGCCTGGTCCCCGGCTATGAAGCCCCGGTGCTGCTGGCCTATTCGGCCCGCAACCGTTCGGCTTCCTGCCGCATCCC
CTACACCACCAACCCGAAGGCCAAGCGCGTCGAAGTCCGCTTCCCGGATCCGATGGCGAACCCCTATCTCGCCTTCGCCG
CGATGCTGATGGCCGGCCTCGACGGCATCAAGAACAAGATCGATCCGGGTCCGGCGATGGACAAGGACCTCTACGATCTG
CCGAAGGAAGAGCTGAAGTCGATCCCGACGGTCTGCGGTTCGCTGCGCGAGGCGCTCGAGAATCTCGACAAGGACCGCGC
CTTCCTCAAGGCCGGCGGCGTGTTCGACGACGACTTCATCGACGCCTATATCGAGCTGAAGATGACCGAAGTGATGCGCT
TCGAAATGACGCCGCACCCGGTCGAGTTCGAGATGTACTACTCGCAGTAA

Upstream 100 bases:

>100_bases
TATCACCTCCAGCCTGATCGCATCACCTTGCGGCCGCGTGCGGCCGCAAACGCACGACCACCTTCAGCATTTACGACGAC
CTTAAGCACAAGGGGTACTC

Downstream 100 bases:

>100_bases
GCCACTCCTCGGCCGGATCGGCCGACGGGCTCGACAACAACCAAGGCGCCCCTCGCGGGGCGCCTTTTTTCGTCGGGCGA
ACGGCCGCTCCATGCGGGCA

Product: glutamine synthetase, type I

Products: NA

Alternate protein names: Glutamate--ammonia ligase I; Glutamine synthetase I; GSI [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MTTAKEVLKSIKDNDVKYVDLRFTDPRGKWQHVTFDVSMIDEEIFAEGTMFDGSSIAGWKAINESDMMLMLDPTTAVIDP
FFAETTMVITCDILEPSTGEPYNRDPRGIAKKAEAQVKAMGVGDSVFVGPEAEFFVFDDVRYSANPYNTGFKLDSSELPT
NSDTEYEGGNLGHRIRTKAGYFPVPPQDSVQDMRSEMLGAMARMGVKVEKHHHEVASAQHELGMKFDTLTHMADQMQIYK
YCIHQVAHIYGKTATFMPKPVFGDNGSGMHVHQSIWKDGKPVFAGNKYADLSETCLHYIGGIISHAKAINAFTNPSTNSY
KRLVPGYEAPVLLAYSARNRSASCRIPYTTNPKAKRVEVRFPDPMANPYLAFAAMLMAGLDGIKNKIDPGPAMDKDLYDL
PKEELKSIPTVCGSLREALENLDKDRAFLKAGGVFDDDFIDAYIELKMTEVMRFEMTPHPVEFEMYYSQ

Sequences:

>Translated_469_residues
MTTAKEVLKSIKDNDVKYVDLRFTDPRGKWQHVTFDVSMIDEEIFAEGTMFDGSSIAGWKAINESDMMLMLDPTTAVIDP
FFAETTMVITCDILEPSTGEPYNRDPRGIAKKAEAQVKAMGVGDSVFVGPEAEFFVFDDVRYSANPYNTGFKLDSSELPT
NSDTEYEGGNLGHRIRTKAGYFPVPPQDSVQDMRSEMLGAMARMGVKVEKHHHEVASAQHELGMKFDTLTHMADQMQIYK
YCIHQVAHIYGKTATFMPKPVFGDNGSGMHVHQSIWKDGKPVFAGNKYADLSETCLHYIGGIISHAKAINAFTNPSTNSY
KRLVPGYEAPVLLAYSARNRSASCRIPYTTNPKAKRVEVRFPDPMANPYLAFAAMLMAGLDGIKNKIDPGPAMDKDLYDL
PKEELKSIPTVCGSLREALENLDKDRAFLKAGGVFDDDFIDAYIELKMTEVMRFEMTPHPVEFEMYYSQ
>Mature_468_residues
TTAKEVLKSIKDNDVKYVDLRFTDPRGKWQHVTFDVSMIDEEIFAEGTMFDGSSIAGWKAINESDMMLMLDPTTAVIDPF
FAETTMVITCDILEPSTGEPYNRDPRGIAKKAEAQVKAMGVGDSVFVGPEAEFFVFDDVRYSANPYNTGFKLDSSELPTN
SDTEYEGGNLGHRIRTKAGYFPVPPQDSVQDMRSEMLGAMARMGVKVEKHHHEVASAQHELGMKFDTLTHMADQMQIYKY
CIHQVAHIYGKTATFMPKPVFGDNGSGMHVHQSIWKDGKPVFAGNKYADLSETCLHYIGGIISHAKAINAFTNPSTNSYK
RLVPGYEAPVLLAYSARNRSASCRIPYTTNPKAKRVEVRFPDPMANPYLAFAAMLMAGLDGIKNKIDPGPAMDKDLYDLP
KEELKSIPTVCGSLREALENLDKDRAFLKAGGVFDDDFIDAYIELKMTEVMRFEMTPHPVEFEMYYSQ

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=416, Percent_Identity=23.7980769230769, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1790301, Length=467, Percent_Identity=64.6680942184154, Blast_Score=635, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=472, Percent_Identity=24.7881355932203, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52463; Mature: 52332

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAKEVLKSIKDNDVKYVDLRFTDPRGKWQHVTFDVSMIDEEIFAEGTMFDGSSIAGWK
CCCHHHHHHHHCCCCEEEEEEEEECCCCCEEEEEEEHHHHHHHHHHCCCCCCCCCCCCCE
AINESDMMLMLDPTTAVIDPFFAETTMVITCDILEPSTGEPYNRDPRGIAKKAEAQVKAM
EECCCCEEEEECCCHHHHCHHHHCEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHEEEE
GVGDSVFVGPEAEFFVFDDVRYSANPYNTGFKLDSSELPTNSDTEYEGGNLGHRIRTKAG
CCCCEEEECCCCCEEEEECCEECCCCCCCCCEECCCCCCCCCCCCCCCCCCCCEEECCCC
YFPVPPQDSVQDMRSEMLGAMARMGVKVEKHHHEVASAQHELGMKFDTLTHMADQMQIYK
CCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
YCIHQVAHIYGKTATFMPKPVFGDNGSGMHVHQSIWKDGKPVFAGNKYADLSETCLHYIG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEECCCCCCCHHHHHHHHHH
GIISHAKAINAFTNPSTNSYKRLVPGYEAPVLLAYSARNRSASCRIPYTTNPKAKRVEVR
HHHHHHHHHHHCCCCCCCCHHHCCCCCCCCEEEEEECCCCCCEEECCCCCCCCCCEEEEE
FPDPMANPYLAFAAMLMAGLDGIKNKIDPGPAMDKDLYDLPKEELKSIPTVCGSLREALE
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHH
NLDKDRAFLKAGGVFDDDFIDAYIELKMTEVMRFEMTPHPVEFEMYYSQ
HCCHHHHHHHCCCCCCHHHHHHHHHEEEHHHHEECCCCCCEEEEEEECC
>Mature Secondary Structure 
TTAKEVLKSIKDNDVKYVDLRFTDPRGKWQHVTFDVSMIDEEIFAEGTMFDGSSIAGWK
CCHHHHHHHHCCCCEEEEEEEEECCCCCEEEEEEEHHHHHHHHHHCCCCCCCCCCCCCE
AINESDMMLMLDPTTAVIDPFFAETTMVITCDILEPSTGEPYNRDPRGIAKKAEAQVKAM
EECCCCEEEEECCCHHHHCHHHHCEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHEEEE
GVGDSVFVGPEAEFFVFDDVRYSANPYNTGFKLDSSELPTNSDTEYEGGNLGHRIRTKAG
CCCCEEEECCCCCEEEEECCEECCCCCCCCCEECCCCCCCCCCCCCCCCCCCCEEECCCC
YFPVPPQDSVQDMRSEMLGAMARMGVKVEKHHHEVASAQHELGMKFDTLTHMADQMQIYK
CCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
YCIHQVAHIYGKTATFMPKPVFGDNGSGMHVHQSIWKDGKPVFAGNKYADLSETCLHYIG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEECCCCCCCHHHHHHHHHH
GIISHAKAINAFTNPSTNSYKRLVPGYEAPVLLAYSARNRSASCRIPYTTNPKAKRVEVR
HHHHHHHHHHHCCCCCCCCHHHCCCCCCCCEEEEEECCCCCCEEECCCCCCCCCCEEEEE
FPDPMANPYLAFAAMLMAGLDGIKNKIDPGPAMDKDLYDLPKEELKSIPTVCGSLREALE
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHH
NLDKDRAFLKAGGVFDDDFIDAYIELKMTEVMRFEMTPHPVEFEMYYSQ
HCCHHHHHHHCCCCCCHHHHHHHHHEEEHHHHEECCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275; 2859270; 2793830 [H]