Click here to switch to the map view.

The map label for this gene is ddh [H]

Identifier: 86749377

GI number: 86749377

Start: 2604466

End: 2605476

Strand: Direct

Name: ddh [H]

Synonym: RPB_2257

Alternate gene names: 86749377

Gene position: 2604466-2605476 (Clockwise)

Preceding gene: 86749372

Following gene: 86749378

Centisome position: 48.85

GC content: 60.24

Gene sequence:

>1011_bases
ATGAAAGTCGCGGTATTCAACACCAAACCCTACGATCGGCAGTTTCTGACGGCGGCCAACACCAAGGACGGGGACAGGCA
CGAATTGCGCTTTCTCGAGCCGCGGCTCGGTCTGGATACGGCGCAACTCAGTGGCAATGCCGATGCGATCTGCGCTTTCG
TCAACGACGGGCTCGACCGATCGATTCTGGAAAGGCTGAAAGACAACGGCGTCCGATTGGTGGCGCTGCGCTGCGCCGGC
TTCAACAATGTGGACCTGTTGGCCGCGCGCGATCTCGAGATCACGGTGGCGCGGGTGCCGGCCTATTCACCGTCCGCCGT
GGCGGAGCACACGGTCGCGCTGATCCTGTCCCTCAACCGCAGAATTCACCGCGCCCATGCGCGCGTGCGCGAAGGCAATT
TCGCCCTGGATGGATTGCTCGGATTCGATCTCAGGGGACGCAGCATCGGCATCGTCGGCACCGGCAACATTGGCCTGTGC
CTGGCCGGCATCATGACGGGATTCGGCTGTCGCGTGCTGGGCTTCGATCCCCGGCCCAATCCTGCCTTCGAGGCGCTCGG
CGGGCGTCTCGTCGGCCTGGAGGAGTTGCTGTCCGAGTCCGACATCGTCTCGCTGCATTGCCCGCTGACCCCGGACACCC
GTCACATGATCGATGAGACCTCGATCGCCAAGATGAAGCCGGGCGTGATGCTGATCAACACCGGACGCGGGGCGCTGATC
GATACGCGGGCGGTGATTCAAGGATTGAAGAACAAGAAGATCGGTTTTCTCGGACTCGACGTCTATGAGGAGGAGAGCGA
TCTGTTCTTCGAAAACCTGTCCGGGCAGATCATTCAGGACGATGATTTTGCGCGATTGCTGACATTTCCCAATGTGCTGA
TCACCGGTCACCAGGCCTTCTTCACGCAGGAGGCTTTGATGGAGATCGCCGATACGACGATCCGGAATATCTCTCAATTC
GAACTGACTGGACAGGCGGCGCATCCGATCTCGTTGGAATTGCGTGTCTGA

Upstream 100 bases:

>100_bases
ACGCAACGCCGCGAGCTGCATAAGACTCGTGGCGTCGGAAACAAGCAGGGATGGCCTGCTATATCTCGCAGCCGGCAGAT
TCGCCGATCGGAGCATGTTT

Downstream 100 bases:

>100_bases
ACGTGACCGAGCTGCTCGCGCTGCTTCCGATCCTGATCCTGCTGCTGTGCATGGTCGGATTTGGCTGGACCGCGGCCCGT
TCCGGCATGATCAGCGCCGC

Product: D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding

Products: pyruvate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MKVAVFNTKPYDRQFLTAANTKDGDRHELRFLEPRLGLDTAQLSGNADAICAFVNDGLDRSILERLKDNGVRLVALRCAG
FNNVDLLAARDLEITVARVPAYSPSAVAEHTVALILSLNRRIHRAHARVREGNFALDGLLGFDLRGRSIGIVGTGNIGLC
LAGIMTGFGCRVLGFDPRPNPAFEALGGRLVGLEELLSESDIVSLHCPLTPDTRHMIDETSIAKMKPGVMLINTGRGALI
DTRAVIQGLKNKKIGFLGLDVYEEESDLFFENLSGQIIQDDDFARLLTFPNVLITGHQAFFTQEALMEIADTTIRNISQF
ELTGQAAHPISLELRV

Sequences:

>Translated_336_residues
MKVAVFNTKPYDRQFLTAANTKDGDRHELRFLEPRLGLDTAQLSGNADAICAFVNDGLDRSILERLKDNGVRLVALRCAG
FNNVDLLAARDLEITVARVPAYSPSAVAEHTVALILSLNRRIHRAHARVREGNFALDGLLGFDLRGRSIGIVGTGNIGLC
LAGIMTGFGCRVLGFDPRPNPAFEALGGRLVGLEELLSESDIVSLHCPLTPDTRHMIDETSIAKMKPGVMLINTGRGALI
DTRAVIQGLKNKKIGFLGLDVYEEESDLFFENLSGQIIQDDDFARLLTFPNVLITGHQAFFTQEALMEIADTTIRNISQF
ELTGQAAHPISLELRV
>Mature_336_residues
MKVAVFNTKPYDRQFLTAANTKDGDRHELRFLEPRLGLDTAQLSGNADAICAFVNDGLDRSILERLKDNGVRLVALRCAG
FNNVDLLAARDLEITVARVPAYSPSAVAEHTVALILSLNRRIHRAHARVREGNFALDGLLGFDLRGRSIGIVGTGNIGLC
LAGIMTGFGCRVLGFDPRPNPAFEALGGRLVGLEELLSESDIVSLHCPLTPDTRHMIDETSIAKMKPGVMLINTGRGALI
DTRAVIQGLKNKKIGFLGLDVYEEESDLFFENLSGQIIQDDDFARLLTFPNVLITGHQAFFTQEALMEIADTTIRNISQF
ELTGQAAHPISLELRV

Specific function: Fermentative Lactate Dehydrogenase. [C]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4557497, Length=269, Percent_Identity=33.8289962825279, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI61743967, Length=269, Percent_Identity=33.8289962825279, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI145580578, Length=270, Percent_Identity=32.2222222222222, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI4557499, Length=270, Percent_Identity=32.2222222222222, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI145580575, Length=270, Percent_Identity=32.2222222222222, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI23308577, Length=187, Percent_Identity=33.6898395721925, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI6912396, Length=271, Percent_Identity=27.3062730627306, Blast_Score=89, Evalue=4e-18,
Organism=Escherichia coli, GI1787645, Length=321, Percent_Identity=53.2710280373832, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI87082289, Length=245, Percent_Identity=31.4285714285714, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1789279, Length=251, Percent_Identity=33.0677290836653, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1788660, Length=279, Percent_Identity=29.3906810035842, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17532191, Length=251, Percent_Identity=30.2788844621514, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI25147481, Length=262, Percent_Identity=29.7709923664122, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6324055, Length=253, Percent_Identity=33.201581027668, Blast_Score=127, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6320925, Length=249, Percent_Identity=32.5301204819277, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6322116, Length=249, Percent_Identity=32.1285140562249, Blast_Score=118, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6324964, Length=213, Percent_Identity=30.5164319248826, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324980, Length=140, Percent_Identity=30, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24646446, Length=272, Percent_Identity=35.6617647058824, Blast_Score=134, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24646448, Length=272, Percent_Identity=35.6617647058824, Blast_Score=134, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24646452, Length=272, Percent_Identity=35.6617647058824, Blast_Score=134, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24646450, Length=272, Percent_Identity=35.6617647058824, Blast_Score=134, Evalue=7e-32,
Organism=Drosophila melanogaster, GI62472511, Length=272, Percent_Identity=35.6617647058824, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI19921140, Length=241, Percent_Identity=31.5352697095436, Blast_Score=120, Evalue=9e-28,
Organism=Drosophila melanogaster, GI28571528, Length=276, Percent_Identity=32.2463768115942, Blast_Score=107, Evalue=8e-24,
Organism=Drosophila melanogaster, GI28574286, Length=266, Percent_Identity=28.5714285714286, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI45552429, Length=266, Percent_Identity=27.4436090225564, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24585514, Length=266, Percent_Identity=27.4436090225564, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI28574282, Length=266, Percent_Identity=27.4436090225564, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI28574284, Length=266, Percent_Identity=27.4436090225564, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI45551003, Length=266, Percent_Identity=27.4436090225564, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24585516, Length=274, Percent_Identity=23.7226277372263, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: 1.1.1.28

Molecular weight: Translated: 36749; Mature: 36749

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAVFNTKPYDRQFLTAANTKDGDRHELRFLEPRLGLDTAQLSGNADAICAFVNDGLDR
CEEEEECCCCCCCEEEEECCCCCCCCCCEEEECCCCCCCHHCCCCCCCEEEEEECCCCCH
SILERLKDNGVRLVALRCAGFNNVDLLAARDLEITVARVPAYSPSAVAEHTVALILSLNR
HHHHHHHCCCEEEEEEEECCCCCEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
RIHRAHARVREGNFALDGLLGFDLRGRSIGIVGTGNIGLCLAGIMTGFGCRVLGFDPRPN
HHHHHHHHHCCCCEEEECEEEECCCCCEEEEEECCCHHHHHHHHHHCCCEEEEECCCCCC
PAFEALGGRLVGLEELLSESDIVSLHCPLTPDTRHMIDETSIAKMKPGVMLINTGRGALI
HHHHHHCCEEECHHHHCCCCCEEEEECCCCCCHHHHHCCCHHHHCCCCEEEEECCCCCEE
DTRAVIQGLKNKKIGFLGLDVYEEESDLFFENLSGQIIQDDDFARLLTFPNVLITGHQAF
EHHHHHHCCCCCCEEEEEEEEEECCCHHHHHCCCCCEEECCCHHHHHHCCCEEEECCHHH
FTQEALMEIADTTIRNISQFELTGQAAHPISLELRV
HHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEEC
>Mature Secondary Structure
MKVAVFNTKPYDRQFLTAANTKDGDRHELRFLEPRLGLDTAQLSGNADAICAFVNDGLDR
CEEEEECCCCCCCEEEEECCCCCCCCCCEEEECCCCCCCHHCCCCCCCEEEEEECCCCCH
SILERLKDNGVRLVALRCAGFNNVDLLAARDLEITVARVPAYSPSAVAEHTVALILSLNR
HHHHHHHCCCEEEEEEEECCCCCEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
RIHRAHARVREGNFALDGLLGFDLRGRSIGIVGTGNIGLCLAGIMTGFGCRVLGFDPRPN
HHHHHHHHHCCCCEEEECEEEECCCCCEEEEEECCCHHHHHHHHHHCCCEEEEECCCCCC
PAFEALGGRLVGLEELLSESDIVSLHCPLTPDTRHMIDETSIAKMKPGVMLINTGRGALI
HHHHHHCCEEECHHHHCCCCCEEEEECCCCCCHHHHHCCCHHHHCCCCEEEEECCCCCEE
DTRAVIQGLKNKKIGFLGLDVYEEESDLFFENLSGQIIQDDDFARLLTFPNVLITGHQAF
EHHHHHHCCCCCCEEEEEEEEEECCCHHHHHCCCCCEEECCCHHHHHHCCCEEEECCHHH
FTQEALMEIADTTIRNISQFELTGQAAHPISLELRV
HHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-lactate; NAD+

Specific reaction: (R)-lactate + NAD+ = pyruvate + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: Pyruvate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]