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The map label for this gene is bam [H]

Identifier: 86749019

GI number: 86749019

Start: 2170314

End: 2171711

Strand: Direct

Name: bam [H]

Synonym: RPB_1896

Alternate gene names: 86749019

Gene position: 2170314-2171711 (Clockwise)

Preceding gene: 86749018

Following gene: 86749020

Centisome position: 40.71

GC content: 70.03

Gene sequence:

>1398_bases
ATGCAGGAAATCTGGCGATTGTCCGCGGCCGACCTGGCCGCATTGATCCGTGCCCGCAAGGTGTCTGCGCGCGAAGCAGC
ACAGGCGGCGCTGGATCGGCTCGATTCCGTCAATCCGGCGATCAATGCGGTGATCGACCATCGCCCCGACGTGACGCTGG
CGCAAGCCGACGCGGTCGACGCCGCGCTTCAGCGCGGCGACGACGCTGGTCTGCTGGCCGGCGTGCCGGTCACGGTCAAG
GTCAATGTCGATCAGCAAGGCTTCGCCACGACCAACGGTCTGACGCAGCAACGCGACCTGATCGCTGCGTCCAACAATCC
GGTGGTCGACAATCTGCGCAAGGCCGGCGCCGTGATCGTCGGTCGCACCAATACGCCGGCGTTCTCCTATCGCTGGTTCA
CGTCGAACAAACTCCACGGCGAAACGCTGAACCCGCGCGATTCCTCGATCACGCCGGGCGGCTCGTCGGGCGGCGCCGGC
GCGGCGGTCGCGGCCGGCATCGGCCATATCGCGCACGGCACCGATATCGCGGGCTCGGTCCGCTATCCCGCCTATGCCTG
CGGCGTTCACGGCCTGCGGCCGACGCTCGGGCGGATCGCCGCCTACAATGCCTCGTCGCCGGAGCGTCCGATCTGTCCGC
AGATCAGCGCGGTGTCGGGCCCCTTGGCGCGGACGATCGGCGATCTGCGGATCGCGCTCGCGGTGATGTCGCAGCCGGAC
GCGCGCGATCCGTGGTGGGTGCCGGCGCCGCTGGACGGCCCGCCGATGCCGAAGCGCGCCGCGCTGTGCATCGCGCCCGA
TGGACTCGAGGTCGTCCCCGAGGTGCGCGACGCGGTGGTCGACGCCGCGCGCCGGCTGGAGCAGGCCGGCTGGAGCGTCG
AGGAATTGCCGAACACGCCGCCGCTGCGTGAGGCTGCCGATCTGCAGGCGCGGCTGTGGCTCGGCGACGGCTATCAGGCG
ATGCTCGCGGCCGCCGAGCGCGAGGGCGACGAGGGCGCGCTGGCCTGCCTCAACGGCAATCGCGACCGGGAAATTCCCGA
TGCTGCGGCATTCTCCAAACTCCTGGTGCGCAGGGCGACGCTGACGCGCGACTGGCAGCTTTTCTTCGAGCGCTACGCGG
TGCTGCTGATGCCGGTGTCGGGCGAATTGCCGTTTCCGCAGCGGCTGGATCTGAAGGACGAAGCCTCGTTCAAGCGGGTG
TGGGCGGCGCAGATGCCCCAGGTCGGCATCCCGTTCATGGGTCTGCCCGGACTGACGGTTGCGACCGGGCTGGTCGGCCG
GGTACCGGTCGGCGTGCAACTGGTCGCCGGCCGCTATCGCGAAGATCTGTGCCTGCTCGCCGGCGAGGCGATCGAGGCCG
GCGGCACGCCGGCGTCGCCGATCGATCCGGTGCGGTAG

Upstream 100 bases:

>100_bases
ATATGCCGCCCTTGCCGCGGAACAGGCATTGATGCGGGTGGATTCACGGTAGCGCCGCATCGGGGGCCGCGCTATCACGT
CATTGTCAACCGAGGGGAAC

Downstream 100 bases:

>100_bases
GGCGGCGACGATGGCGTCGATTTATGATTTTTCGGCCAGGACGCTTGCGGGCGAAGAGCTGCCGCTGAAGCGGTTCGAGG
GGCAGGTGCTGCTGATCGTC

Product: amidase

Products: NA

Alternate protein names: IAH; Indole-3-acetamide hydrolase [H]

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MQEIWRLSAADLAALIRARKVSAREAAQAALDRLDSVNPAINAVIDHRPDVTLAQADAVDAALQRGDDAGLLAGVPVTVK
VNVDQQGFATTNGLTQQRDLIAASNNPVVDNLRKAGAVIVGRTNTPAFSYRWFTSNKLHGETLNPRDSSITPGGSSGGAG
AAVAAGIGHIAHGTDIAGSVRYPAYACGVHGLRPTLGRIAAYNASSPERPICPQISAVSGPLARTIGDLRIALAVMSQPD
ARDPWWVPAPLDGPPMPKRAALCIAPDGLEVVPEVRDAVVDAARRLEQAGWSVEELPNTPPLREAADLQARLWLGDGYQA
MLAAAEREGDEGALACLNGNRDREIPDAAAFSKLLVRRATLTRDWQLFFERYAVLLMPVSGELPFPQRLDLKDEASFKRV
WAAQMPQVGIPFMGLPGLTVATGLVGRVPVGVQLVAGRYREDLCLLAGEAIEAGGTPASPIDPVR

Sequences:

>Translated_465_residues
MQEIWRLSAADLAALIRARKVSAREAAQAALDRLDSVNPAINAVIDHRPDVTLAQADAVDAALQRGDDAGLLAGVPVTVK
VNVDQQGFATTNGLTQQRDLIAASNNPVVDNLRKAGAVIVGRTNTPAFSYRWFTSNKLHGETLNPRDSSITPGGSSGGAG
AAVAAGIGHIAHGTDIAGSVRYPAYACGVHGLRPTLGRIAAYNASSPERPICPQISAVSGPLARTIGDLRIALAVMSQPD
ARDPWWVPAPLDGPPMPKRAALCIAPDGLEVVPEVRDAVVDAARRLEQAGWSVEELPNTPPLREAADLQARLWLGDGYQA
MLAAAEREGDEGALACLNGNRDREIPDAAAFSKLLVRRATLTRDWQLFFERYAVLLMPVSGELPFPQRLDLKDEASFKRV
WAAQMPQVGIPFMGLPGLTVATGLVGRVPVGVQLVAGRYREDLCLLAGEAIEAGGTPASPIDPVR
>Mature_465_residues
MQEIWRLSAADLAALIRARKVSAREAAQAALDRLDSVNPAINAVIDHRPDVTLAQADAVDAALQRGDDAGLLAGVPVTVK
VNVDQQGFATTNGLTQQRDLIAASNNPVVDNLRKAGAVIVGRTNTPAFSYRWFTSNKLHGETLNPRDSSITPGGSSGGAG
AAVAAGIGHIAHGTDIAGSVRYPAYACGVHGLRPTLGRIAAYNASSPERPICPQISAVSGPLARTIGDLRIALAVMSQPD
ARDPWWVPAPLDGPPMPKRAALCIAPDGLEVVPEVRDAVVDAARRLEQAGWSVEELPNTPPLREAADLQARLWLGDGYQA
MLAAAEREGDEGALACLNGNRDREIPDAAAFSKLLVRRATLTRDWQLFFERYAVLLMPVSGELPFPQRLDLKDEASFKRV
WAAQMPQVGIPFMGLPGLTVATGLVGRVPVGVQLVAGRYREDLCLLAGEAIEAGGTPASPIDPVR

Specific function: Hydrolyzes indole-3-acetamide (IAM) into indole-3-acetic acid (IAA) [H]

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=243, Percent_Identity=36.6255144032922, Blast_Score=145, Evalue=8e-35,
Organism=Homo sapiens, GI222831590, Length=503, Percent_Identity=27.2365805168986, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI166795287, Length=304, Percent_Identity=31.5789473684211, Blast_Score=82, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17538252, Length=262, Percent_Identity=33.206106870229, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17537465, Length=499, Percent_Identity=25.4509018036072, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17556264, Length=312, Percent_Identity=29.8076923076923, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17556276, Length=361, Percent_Identity=28.5318559556787, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17556278, Length=361, Percent_Identity=28.5318559556787, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71990152, Length=161, Percent_Identity=37.888198757764, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17538254, Length=304, Percent_Identity=25.3289473684211, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=253, Percent_Identity=31.6205533596838, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320448, Length=245, Percent_Identity=29.3877551020408, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45550774, Length=246, Percent_Identity=33.739837398374, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24648435, Length=246, Percent_Identity=33.739837398374, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24648437, Length=246, Percent_Identity=33.739837398374, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24652985, Length=486, Percent_Identity=26.7489711934156, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI19922090, Length=486, Percent_Identity=26.7489711934156, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24652981, Length=486, Percent_Identity=26.7489711934156, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24652983, Length=486, Percent_Identity=26.7489711934156, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24644968, Length=500, Percent_Identity=23.6, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI21356731, Length=504, Percent_Identity=26.3888888888889, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI161078093, Length=494, Percent_Identity=25.5060728744939, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24648441, Length=170, Percent_Identity=35.2941176470588, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24648439, Length=170, Percent_Identity=35.2941176470588, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24648113, Length=466, Percent_Identity=25.3218884120172, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 49232; Mature: 49232

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEIWRLSAADLAALIRARKVSAREAAQAALDRLDSVNPAINAVIDHRPDVTLAQADAVD
CCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEHHHHHH
AALQRGDDAGLLAGVPVTVKVNVDQQGFATTNGLTQQRDLIAASNNPVVDNLRKAGAVIV
HHHHCCCCCCEEECCCEEEEEECCCCCCCCCCCCCHHHHEEECCCCCHHHHHHHCCEEEE
GRTNTPAFSYRWFTSNKLHGETLNPRDSSITPGGSSGGAGAAVAAGIGHIAHGTDIAGSV
ECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCC
RYPAYACGVHGLRPTLGRIAAYNASSPERPICPQISAVSGPLARTIGDLRIALAVMSQPD
CCCHHHHCCCCCCHHHHHHEEECCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHCCCC
ARDPWWVPAPLDGPPMPKRAALCIAPDGLEVVPEVRDAVVDAARRLEQAGWSVEELPNTP
CCCCCEECCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCC
PLREAADLQARLWLGDGYQAMLAAAEREGDEGALACLNGNRDREIPDAAAFSKLLVRRAT
CCHHHHCCEEEEEECCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHH
LTRDWQLFFERYAVLLMPVSGELPFPQRLDLKDEASFKRVWAAQMPQVGIPFMGLPGLTV
HHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHH
ATGLVGRVPVGVQLVAGRYREDLCLLAGEAIEAGGTPASPIDPVR
HHHHHHCCCCCEEEECCHHHHHHHHHCCCHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MQEIWRLSAADLAALIRARKVSAREAAQAALDRLDSVNPAINAVIDHRPDVTLAQADAVD
CCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEHHHHHH
AALQRGDDAGLLAGVPVTVKVNVDQQGFATTNGLTQQRDLIAASNNPVVDNLRKAGAVIV
HHHHCCCCCCEEECCCEEEEEECCCCCCCCCCCCCHHHHEEECCCCCHHHHHHHCCEEEE
GRTNTPAFSYRWFTSNKLHGETLNPRDSSITPGGSSGGAGAAVAAGIGHIAHGTDIAGSV
ECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCC
RYPAYACGVHGLRPTLGRIAAYNASSPERPICPQISAVSGPLARTIGDLRIALAVMSQPD
CCCHHHHCCCCCCHHHHHHEEECCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHCCCC
ARDPWWVPAPLDGPPMPKRAALCIAPDGLEVVPEVRDAVVDAARRLEQAGWSVEELPNTP
CCCCCEECCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCC
PLREAADLQARLWLGDGYQAMLAAAEREGDEGALACLNGNRDREIPDAAAFSKLLVRRAT
CCHHHHCCEEEEEECCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHH
LTRDWQLFFERYAVLLMPVSGELPFPQRLDLKDEASFKRVWAAQMPQVGIPFMGLPGLTV
HHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHH
ATGLVGRVPVGVQLVAGRYREDLCLLAGEAIEAGGTPASPIDPVR
HHHHHHCCCCCEEEECCHHHHHHHHHCCCHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]