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The map label for this gene is waaA [H]

Identifier: 86748978

GI number: 86748978

Start: 2120158

End: 2121504

Strand: Direct

Name: waaA [H]

Synonym: RPB_1855

Alternate gene names: 86748978

Gene position: 2120158-2121504 (Clockwise)

Preceding gene: 86748977

Following gene: 86748979

Centisome position: 39.77

GC content: 70.08

Gene sequence:

>1347_bases
ATGACGAGCGCGTCACCTCCGCGGTGGCAGACGGGGCCTTTGCTGGCCGAACCGTTGCCGATGACGCTGCGCGTCTATCA
GCAGCTCACCGCTGGTATCTCCCCGCTCGCCCCGCTGCTGATCCAGCGGCGGCTCAAGCAGGGCAAGGAAGAGCCGGCGC
GGGTCGACGAGCGCCGCGGTGTCGCCGCGCATGTGCGGCCACACGGACCGCTGGTGTGGATCCACGGCGCCAGCGTCGGC
GAGGTGCTGGCCGCGGCCGGACTGATCGAGCGGCTGCGCGCGCTCAACCTGCGCATCCTGCTGACGTCCGGCACCGTGAC
CTCGGCAGCCGTGGTGGCGAAACGGTTTCCGCCCGACATCATCCATCAGTTCATCCCCTATGACGCGCCGCGCTTCGTCG
CGCGCTTCCTCGATCACTGGCAGCCGTCATTGGCGCTGTTCGTCGAATCCGACCTGTGGCCGAATCTGATCCTCGCCTCC
GCGGCGCGGCGGTTGCCGATGGTGCTGATCAACGGCCGGATGTCGCAGCGCTCGTTCCCGCGCTGGCGGCGCGCCGCAGC
GACGATCGGCACGCTGCTCGGCAAGTTCGACATCTGCCTCGCGCAATCGCGGATGGACGCCGAGCGGTTCGCGGCGCTGG
GCAGCCGCAACGTCATCACCACCGGCAATCTCAAGATGGACGTCGACCCCCCGCCGGGCGATCCGGCGCGGCTGGAACGG
CTGATGGCGGTGACGCGCGGCCGGCAGGTGATCGTCGCCGCCTCGACCCATCCGGGCGAAGAGGAGATCCTGCTCGACGT
CCACCGCAGGCTTGCCGGGGCGTTCCCGGCGCTGCTCACCGTGATCGTGCCGCGGCATCCGCATCGCGGCGAGCAGATCG
CTGGCCTGATTGAGGCCTCCGGCCTGCACGCGGCGTTGCGCTCGCGCGAGCAACTGCCGACCGCGGCGACGGCGATCTAC
GTCGCCGACACCATGGGCGAGCTCGGCCTATTCTACCGGCTGGCGCCGATCGTGTTCATGGGCGGCTCGCTGATCGAGCA
CGGCGGCCAGAATCCGATCGAGGCGGTCAAGCTCGGCGCCTCGATCGTGCACGGACCGCACGTCTCCAATTTCACCGACG
TCTATCGCGCGCTCGACGACGAGGGCGGTGCCTTCACGGCGGCCGACGCCGACGCGCTGGTGCGGCGGTTCGGCCAGTTG
CTGTCCGACAGCAATGCGCGCCAGACCTCGATCGATGCCGCCACCCGCGTGGTCGATCGGCTCGGCGGCGCGCTGGACCG
CACCGTCGCGGCGCTGGAACCCTATCTGCTGCAATTGCGCATCGAGCAGGGCGCCGCCGGTGCGTGA

Upstream 100 bases:

>100_bases
GTCGACAACGCCCATATGCGCTACTTCATCGTTCATCTCCATCGCCTGCTGGATCCCGGCCAGGACGCAAAGCTGGTAAG
TTGACCAGGCTTGCAGTGCG

Downstream 100 bases:

>100_bases
GCCGGGCTTCTGGCACAGGCCGCCTTCTTGGCTGTCGCGTCTGCTGCTGCCGCTCGGCGCCGTGTATGGCGAAATCACCT
CCTGGCGAATGCGCAAGACC

Product: three-deoxy-D-manno-octulosonic-acid transferase-like

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MTSASPPRWQTGPLLAEPLPMTLRVYQQLTAGISPLAPLLIQRRLKQGKEEPARVDERRGVAAHVRPHGPLVWIHGASVG
EVLAAAGLIERLRALNLRILLTSGTVTSAAVVAKRFPPDIIHQFIPYDAPRFVARFLDHWQPSLALFVESDLWPNLILAS
AARRLPMVLINGRMSQRSFPRWRRAAATIGTLLGKFDICLAQSRMDAERFAALGSRNVITTGNLKMDVDPPPGDPARLER
LMAVTRGRQVIVAASTHPGEEEILLDVHRRLAGAFPALLTVIVPRHPHRGEQIAGLIEASGLHAALRSREQLPTAATAIY
VADTMGELGLFYRLAPIVFMGGSLIEHGGQNPIEAVKLGASIVHGPHVSNFTDVYRALDDEGGAFTAADADALVRRFGQL
LSDSNARQTSIDAATRVVDRLGGALDRTVAALEPYLLQLRIEQGAAGA

Sequences:

>Translated_448_residues
MTSASPPRWQTGPLLAEPLPMTLRVYQQLTAGISPLAPLLIQRRLKQGKEEPARVDERRGVAAHVRPHGPLVWIHGASVG
EVLAAAGLIERLRALNLRILLTSGTVTSAAVVAKRFPPDIIHQFIPYDAPRFVARFLDHWQPSLALFVESDLWPNLILAS
AARRLPMVLINGRMSQRSFPRWRRAAATIGTLLGKFDICLAQSRMDAERFAALGSRNVITTGNLKMDVDPPPGDPARLER
LMAVTRGRQVIVAASTHPGEEEILLDVHRRLAGAFPALLTVIVPRHPHRGEQIAGLIEASGLHAALRSREQLPTAATAIY
VADTMGELGLFYRLAPIVFMGGSLIEHGGQNPIEAVKLGASIVHGPHVSNFTDVYRALDDEGGAFTAADADALVRRFGQL
LSDSNARQTSIDAATRVVDRLGGALDRTVAALEPYLLQLRIEQGAAGA
>Mature_447_residues
TSASPPRWQTGPLLAEPLPMTLRVYQQLTAGISPLAPLLIQRRLKQGKEEPARVDERRGVAAHVRPHGPLVWIHGASVGE
VLAAAGLIERLRALNLRILLTSGTVTSAAVVAKRFPPDIIHQFIPYDAPRFVARFLDHWQPSLALFVESDLWPNLILASA
ARRLPMVLINGRMSQRSFPRWRRAAATIGTLLGKFDICLAQSRMDAERFAALGSRNVITTGNLKMDVDPPPGDPARLERL
MAVTRGRQVIVAASTHPGEEEILLDVHRRLAGAFPALLTVIVPRHPHRGEQIAGLIEASGLHAALRSREQLPTAATAIYV
ADTMGELGLFYRLAPIVFMGGSLIEHGGQNPIEAVKLGASIVHGPHVSNFTDVYRALDDEGGAFTAADADALVRRFGQLL
SDSNARQTSIDAATRVVDRLGGALDRTVAALEPYLLQLRIEQGAAGA

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=421, Percent_Identity=38.2422802850356, Blast_Score=259, Evalue=2e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 48540; Mature: 48409

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSASPPRWQTGPLLAEPLPMTLRVYQQLTAGISPLAPLLIQRRLKQGKEEPARVDERRG
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHCC
VAAHVRPHGPLVWIHGASVGEVLAAAGLIERLRALNLRILLTSGTVTSAAVVAKRFPPDI
CEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCHHH
IHQFIPYDAPRFVARFLDHWQPSLALFVESDLWPNLILASAARRLPMVLINGRMSQRSFP
HHHHCCCCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCEEEECCCCCCCCCH
RWRRAAATIGTLLGKFDICLAQSRMDAERFAALGSRNVITTGNLKMDVDPPPGDPARLER
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEECCEEEECCCCCCCHHHHHH
LMAVTRGRQVIVAASTHPGEEEILLDVHRRLAGAFPALLTVIVPRHPHRGEQIAGLIEAS
HHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
GLHAALRSREQLPTAATAIYVADTMGELGLFYRLAPIVFMGGSLIEHGGQNPIEAVKLGA
HHHHHHHHHHHCCHHHHHEEEHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHH
SIVHGPHVSNFTDVYRALDDEGGAFTAADADALVRRFGQLLSDSNARQTSIDAATRVVDR
HHCCCCCCCHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LGGALDRTVAALEPYLLQLRIEQGAAGA
HCCHHHHHHHHHHHHHEEEEECCCCCCC
>Mature Secondary Structure 
TSASPPRWQTGPLLAEPLPMTLRVYQQLTAGISPLAPLLIQRRLKQGKEEPARVDERRG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHCC
VAAHVRPHGPLVWIHGASVGEVLAAAGLIERLRALNLRILLTSGTVTSAAVVAKRFPPDI
CEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCHHH
IHQFIPYDAPRFVARFLDHWQPSLALFVESDLWPNLILASAARRLPMVLINGRMSQRSFP
HHHHCCCCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCEEEECCCCCCCCCH
RWRRAAATIGTLLGKFDICLAQSRMDAERFAALGSRNVITTGNLKMDVDPPPGDPARLER
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEECCEEEECCCCCCCHHHHHH
LMAVTRGRQVIVAASTHPGEEEILLDVHRRLAGAFPALLTVIVPRHPHRGEQIAGLIEAS
HHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
GLHAALRSREQLPTAATAIYVADTMGELGLFYRLAPIVFMGGSLIEHGGQNPIEAVKLGA
HHHHHHHHHHHCCHHHHHEEEHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHH
SIVHGPHVSNFTDVYRALDDEGGAFTAADADALVRRFGQLLSDSNARQTSIDAATRVVDR
HHCCCCCCCHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LGGALDRTVAALEPYLLQLRIEQGAAGA
HCCHHHHHHHHHHHHHEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]