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The map label for this gene is hpcH [H]

Identifier: 86748834

GI number: 86748834

Start: 1946174

End: 1946950

Strand: Direct

Name: hpcH [H]

Synonym: RPB_1711

Alternate gene names: 86748834

Gene position: 1946174-1946950 (Clockwise)

Preceding gene: 86748833

Following gene: 86748835

Centisome position: 36.5

GC content: 63.71

Gene sequence:

>777_bases
ATGGCAAACAAGGTGAAACAGCTCTGGGCCTCCGGCAAGGCGGTGGTGAATGCGTGGCTGGCGATCCCGTCCGGCTTCTC
GGCCGAGGTGATGGCGCAATGCGGCTTCGACAGCGTGACCGTCGACATCCAGCACGGCGTGCAGGACTATCAATCGATGG
TGGCCTGTTTTCAGGCGATGAACGGCCATCCGGTGACGCCGATGGTGCGGGTGCCTTGGAACGAGCCGGGCATCATCGGC
AAGGTGCTGGACGGCGGCGCCTATGGGGTCATCTGCCCGATGGTGAATACCAAGGAGGAGGCCGAGGCATTCGTGCAATC
TTGCAAATATCCGCCGCGCGGCACCCGTAGTAACGGGCCGATCCGCGCCGGGATGTATGGCGCCGGTGGCAGCTATCAGG
ACACGGCCAACGTCGAGACGCTGTGCATCCCGATGATCGAGACCCGCACTGCGGTCGAGAACATGGAGGCGATCCTCGAC
GTCGAAGGCATCGCCGGCGTCTATGTCGGGCCGAGCGACCTCGGCTATTCCTACGGGCTGGTGCCGAAGCTCGACCGCGA
GGAGCCGGAGATCCTGAAGATCTACGACAAATTGCTGAAGGAATGCGACAAGCGCGGCATCTACCCGGGCATCCATTGTT
CGGGCCCGGCCGGCGCCGCCAAGAACATCGCGATGGGCTTCAAGCTGGTCACGCTGCTCAACGACAGCGGCATCATGGCG
ACCGCCGCGAAGCAATGGGTCGGCGAGACGCGGCAGAATTCGGGCGGCAAGGCGTAG

Upstream 100 bases:

>100_bases
AGTGCCCGGACGGCGCAAAGCGCCGATCCGGGTGCTTCTCGCAATGACGGAGAGAGGATCTCGATAACTCATAACTGACT
GCGGCAGGAAAGAACTGGCA

Downstream 100 bases:

>100_bases
CTGCTTCGCAACAGTGGTCATCCTCCGCGAAAGCGGAGGTTCCAGTATCCACAGCGCGACCGGATAGCCCGAACACCGGC
GCTGTGGATACTGGGTCGTC

Product: HpcH/HpaI aldolase

Products: NA

Alternate protein names: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase; HHED aldolase; 4-hydroxy-2-ketoheptane-1,7-dioate aldolase; HKHD aldolase [H]

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MANKVKQLWASGKAVVNAWLAIPSGFSAEVMAQCGFDSVTVDIQHGVQDYQSMVACFQAMNGHPVTPMVRVPWNEPGIIG
KVLDGGAYGVICPMVNTKEEAEAFVQSCKYPPRGTRSNGPIRAGMYGAGGSYQDTANVETLCIPMIETRTAVENMEAILD
VEGIAGVYVGPSDLGYSYGLVPKLDREEPEILKIYDKLLKECDKRGIYPGIHCSGPAGAAKNIAMGFKLVTLLNDSGIMA
TAAKQWVGETRQNSGGKA

Sequences:

>Translated_258_residues
MANKVKQLWASGKAVVNAWLAIPSGFSAEVMAQCGFDSVTVDIQHGVQDYQSMVACFQAMNGHPVTPMVRVPWNEPGIIG
KVLDGGAYGVICPMVNTKEEAEAFVQSCKYPPRGTRSNGPIRAGMYGAGGSYQDTANVETLCIPMIETRTAVENMEAILD
VEGIAGVYVGPSDLGYSYGLVPKLDREEPEILKIYDKLLKECDKRGIYPGIHCSGPAGAAKNIAMGFKLVTLLNDSGIMA
TAAKQWVGETRQNSGGKA
>Mature_257_residues
ANKVKQLWASGKAVVNAWLAIPSGFSAEVMAQCGFDSVTVDIQHGVQDYQSMVACFQAMNGHPVTPMVRVPWNEPGIIGK
VLDGGAYGVICPMVNTKEEAEAFVQSCKYPPRGTRSNGPIRAGMYGAGGSYQDTANVETLCIPMIETRTAVENMEAILDV
EGIAGVYVGPSDLGYSYGLVPKLDREEPEILKIYDKLLKECDKRGIYPGIHCSGPAGAAKNIAMGFKLVTLLNDSGIMAT
AAKQWVGETRQNSGGKA

Specific function: Catalyzes the reversible retro-aldol cleavage of 4- hydroxy-2-ketoheptane-1,7-dioate (HKHD) to pyruvate and succinic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=244, Percent_Identity=32.7868852459016, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1788578, Length=259, Percent_Identity=29.7297297297297, Blast_Score=121, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR012689
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.2.-

Molecular weight: Translated: 27554; Mature: 27423

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANKVKQLWASGKAVVNAWLAIPSGFSAEVMAQCGFDSVTVDIQHGVQDYQSMVACFQAM
CCHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHC
NGHPVTPMVRVPWNEPGIIGKVLDGGAYGVICPMVNTKEEAEAFVQSCKYPPRGTRSNGP
CCCCCCCEEECCCCCCCCEEEEECCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCC
IRAGMYGAGGSYQDTANVETLCIPMIETRTAVENMEAILDVEGIAGVYVGPSDLGYSYGL
EEEEEECCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCEEEECCHHCCCCCCC
VPKLDREEPEILKIYDKLLKECDKRGIYPGIHCSGPAGAAKNIAMGFKLVTLLNDSGIMA
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHEEEEEEECCCCCHH
TAAKQWVGETRQNSGGKA
HHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ANKVKQLWASGKAVVNAWLAIPSGFSAEVMAQCGFDSVTVDIQHGVQDYQSMVACFQAM
CHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHC
NGHPVTPMVRVPWNEPGIIGKVLDGGAYGVICPMVNTKEEAEAFVQSCKYPPRGTRSNGP
CCCCCCCEEECCCCCCCCEEEEECCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCC
IRAGMYGAGGSYQDTANVETLCIPMIETRTAVENMEAILDVEGIAGVYVGPSDLGYSYGL
EEEEEECCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCEEEECCHHCCCCCCC
VPKLDREEPEILKIYDKLLKECDKRGIYPGIHCSGPAGAAKNIAMGFKLVTLLNDSGIMA
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHEEEEEEECCCCCHH
TAAKQWVGETRQNSGGKA
HHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA