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The map label for this gene is rfbB [C]

Identifier: 86748679

GI number: 86748679

Start: 1751867

End: 1752883

Strand: Direct

Name: rfbB [C]

Synonym: RPB_1554

Alternate gene names: 86748679

Gene position: 1751867-1752883 (Clockwise)

Preceding gene: 86748678

Following gene: 86748684

Centisome position: 32.86

GC content: 61.16

Gene sequence:

>1017_bases
ATGGGAACTCCGCCCGTTCTGGTGACCGGCGCTGCCGGCTTTATCGGCTTCCACGTCACGCAGCAATTGCTGGCCAAGGG
AAGCAAGGTCGTCGGCCTCGACGTTCTGAACGACTACTACGATCCGGCGCTGAAGCGGGCGCGGCTCGACATCCTGCAGC
GCAATCCGGATTTCACCTTCGTGAAGCTCGATCTCGCGGATCGCGGGGCGATCAAGGCGCTCTTCGCAGAGTACCGGTTC
GCCGTGGTGATTCACCTCGCAGCGCAGGCCGGGGTTCGCTACTCGATCGAAAATCCCTATGCCTATATCGATTCCAACCT
CGAGGGTTTCATCAACGTGCTCGAGGGCTGTCGGCACAACGGATGCCGGCATCTGCTGTATGCGTCGTCCTCGTCGGTCT
ATGGGGCCAATACGAAGCTGCCGTTTGCGGTCCAGGACAATGTCGATCACCCGATCAGTCTGTACGCGGCCACGAAGAAG
GCCAATGAGCTGATGGCGCATGCCTACAGCCATCTGTATCGAATCCCGACCACCGGGCTGCGGTTCTTCACCGTTTATGG
TCCGTGGGGGCGGCCCGATATGGCGATGTTCATCTTCGCCAAGGCCATTACGGAAGGCGCCCCGATCAAGCTGTTCAACC
GCGGCATGATGCGCCGCGACTTCACCTATGTGGACGACGTGAGCGAGGCCATCGTCAGGCTGGTGGACAAGCCGCCACAA
GGCAATGCGGCTTGGTCCGGCGATCATCCGGATCCGGCGAGCAGCACCGCGCCGTGGAAGATCTACAATATCGGCAACAA
CAACCCGGAAGAACTGCTCCACGTGGTTTCGCTGCTGGAGAAGGAATTTGGGCGTCCGGCCCAGAAGGAGATGCTGCCGA
TGCAGCCCGGGGACGTGCCTGCAACCTACGCCGACGTCGAGGATCTGATGCGGGACATCGGATTCCGCCCGTCGACGACG
ATCGAGCGTGGCGTCGCCGCATTCGCGGCGTGGTATCGAGCGTATCACAAGCTCTGA

Upstream 100 bases:

>100_bases
ACGGCGGCAAGGGCATCGTGTTCGACGTCAAGGCCAAATTGGATCGAAGCGCAAAGCCCGCCGATATCGAGCTGCTGCGG
CTCTAACGAAAGCGTCATTC

Downstream 100 bases:

>100_bases
TCTGTCGAGGCGAACCTGTCTCGACGGCCGCATCGGCCGGCGCGGCCGACCGATCGAACGCGCCGATGTCGCGTGCCGGA
TCTCTTTGCATCGAGCGGTC

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MGTPPVLVTGAAGFIGFHVTQQLLAKGSKVVGLDVLNDYYDPALKRARLDILQRNPDFTFVKLDLADRGAIKALFAEYRF
AVVIHLAAQAGVRYSIENPYAYIDSNLEGFINVLEGCRHNGCRHLLYASSSSVYGANTKLPFAVQDNVDHPISLYAATKK
ANELMAHAYSHLYRIPTTGLRFFTVYGPWGRPDMAMFIFAKAITEGAPIKLFNRGMMRRDFTYVDDVSEAIVRLVDKPPQ
GNAAWSGDHPDPASSTAPWKIYNIGNNNPEELLHVVSLLEKEFGRPAQKEMLPMQPGDVPATYADVEDLMRDIGFRPSTT
IERGVAAFAAWYRAYHKL

Sequences:

>Translated_338_residues
MGTPPVLVTGAAGFIGFHVTQQLLAKGSKVVGLDVLNDYYDPALKRARLDILQRNPDFTFVKLDLADRGAIKALFAEYRF
AVVIHLAAQAGVRYSIENPYAYIDSNLEGFINVLEGCRHNGCRHLLYASSSSVYGANTKLPFAVQDNVDHPISLYAATKK
ANELMAHAYSHLYRIPTTGLRFFTVYGPWGRPDMAMFIFAKAITEGAPIKLFNRGMMRRDFTYVDDVSEAIVRLVDKPPQ
GNAAWSGDHPDPASSTAPWKIYNIGNNNPEELLHVVSLLEKEFGRPAQKEMLPMQPGDVPATYADVEDLMRDIGFRPSTT
IERGVAAFAAWYRAYHKL
>Mature_337_residues
GTPPVLVTGAAGFIGFHVTQQLLAKGSKVVGLDVLNDYYDPALKRARLDILQRNPDFTFVKLDLADRGAIKALFAEYRFA
VVIHLAAQAGVRYSIENPYAYIDSNLEGFINVLEGCRHNGCRHLLYASSSSVYGANTKLPFAVQDNVDHPISLYAATKKA
NELMAHAYSHLYRIPTTGLRFFTVYGPWGRPDMAMFIFAKAITEGAPIKLFNRGMMRRDFTYVDDVSEAIVRLVDKPPQG
NAAWSGDHPDPASSTAPWKIYNIGNNNPEELLHVVSLLEKEFGRPAQKEMLPMQPGDVPATYADVEDLMRDIGFRPSTTI
ERGVAAFAAWYRAYHKL

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=336, Percent_Identity=25.297619047619, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI56237023, Length=351, Percent_Identity=25.3561253561254, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI56118217, Length=351, Percent_Identity=25.3561253561254, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI189083684, Length=351, Percent_Identity=25.3561253561254, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI42516563, Length=335, Percent_Identity=23.2835820895522, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI1788353, Length=355, Percent_Identity=26.7605633802817, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1786974, Length=342, Percent_Identity=26.3157894736842, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI48994969, Length=351, Percent_Identity=25.6410256410256, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1788589, Length=283, Percent_Identity=27.5618374558304, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71982035, Length=350, Percent_Identity=26.2857142857143, Blast_Score=107, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI71982038, Length=352, Percent_Identity=26.4204545454545, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17568069, Length=349, Percent_Identity=26.3610315186246, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17539532, Length=237, Percent_Identity=26.5822784810127, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI115532424, Length=350, Percent_Identity=23.7142857142857, Blast_Score=75, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6319493, Length=137, Percent_Identity=35.7664233576642, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19923002, Length=354, Percent_Identity=26.271186440678, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21356223, Length=337, Percent_Identity=23.1454005934718, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 37603; Mature: 37472

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTPPVLVTGAAGFIGFHVTQQLLAKGSKVVGLDVLNDYYDPALKRARLDILQRNPDFTF
CCCCCEEEECCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VKLDLADRGAIKALFAEYRFAVVIHLAAQAGVRYSIENPYAYIDSNLEGFINVLEGCRHN
EEEECCCCHHHHHHHHHHHHHHHHEEHHHCCCEEEECCCEEEECCCHHHHHHHHHHHHCC
GCRHLLYASSSSVYGANTKLPFAVQDNVDHPISLYAATKKANELMAHAYSHLYRIPTTGL
CCEEEEEECCCCCCCCCCCCCEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCE
RFFTVYGPWGRPDMAMFIFAKAITEGAPIKLFNRGMMRRDFTYVDDVSEAIVRLVDKPPQ
EEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHCCHHHCCCHHHHHHHHHHHHHHCCCCC
GNAAWSGDHPDPASSTAPWKIYNIGNNNPEELLHVVSLLEKEFGRPAQKEMLPMQPGDVP
CCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCC
ATYADVEDLMRDIGFRPSTTIERGVAAFAAWYRAYHKL
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GTPPVLVTGAAGFIGFHVTQQLLAKGSKVVGLDVLNDYYDPALKRARLDILQRNPDFTF
CCCCEEEECCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VKLDLADRGAIKALFAEYRFAVVIHLAAQAGVRYSIENPYAYIDSNLEGFINVLEGCRHN
EEEECCCCHHHHHHHHHHHHHHHHEEHHHCCCEEEECCCEEEECCCHHHHHHHHHHHHCC
GCRHLLYASSSSVYGANTKLPFAVQDNVDHPISLYAATKKANELMAHAYSHLYRIPTTGL
CCEEEEEECCCCCCCCCCCCCEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCE
RFFTVYGPWGRPDMAMFIFAKAITEGAPIKLFNRGMMRRDFTYVDDVSEAIVRLVDKPPQ
EEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHCCHHHCCCHHHHHHHHHHHHHHCCCCC
GNAAWSGDHPDPASSTAPWKIYNIGNNNPEELLHVVSLLEKEFGRPAQKEMLPMQPGDVP
CCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCC
ATYADVEDLMRDIGFRPSTTIERGVAAFAAWYRAYHKL
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7682279 [H]