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The map label for this gene is asnB [H]

Identifier: 86748666

GI number: 86748666

Start: 1735865

End: 1737760

Strand: Direct

Name: asnB [H]

Synonym: RPB_1541

Alternate gene names: 86748666

Gene position: 1735865-1737760 (Clockwise)

Preceding gene: 86748665

Following gene: 86748668

Centisome position: 32.56

GC content: 64.82

Gene sequence:

>1896_bases
ATGTGTGGATTGGCGGGCGGCATCTGGCGCGACGACAGACTGCTGGCACCATCGATCGGCGCCGCTGCGCTCGCCGCCCT
GGCGCATCGCGGGCCCGACGCCGAGGGAGAACACCGCGAAGCCGGCGTCTTTCTGGGGCACCGGCGGCTCAGCATCATCG
ATCTCGATCCCCGATCGACGCAGCCGATGCATCTGGGGCCGCTGAGCATCGTCTTCAACGGGGAAATCTACAACTACCGC
GCGCTGCGTGCGGAGCTCGAGCGTGGCGGCTGTGTATTCCGCACGGAATCCGACACGGAAGTTCTGCTCCAGGCCTTCGC
GCGCGACGGACTCGATTGCCTGCAGACCCTGGAAGGGATGTTCTCGTTTGCGATCTGGGACCGCAGCGCGCGGCGCCTGA
CGCTGGCGCGCGATCGCTACGGCGAAAAGCCGATGGTTGTGTTCGAGGATGGCGAGAAGCTGCTGTTCGCGTCCGAGATT
CCGGCGATCGAACGGCTGGCGGGCGACGGCGATCTCGTGATCGATCGGGCCGCCTTGCCGCTGCTGTTCCGCTTTTCCTA
TTTGCCGGCGCCGTTCACGCCGTTCGAGCGGATGAAGCAGATCAAGCCCGGCTGCTGGCTGCAACTGACCGTCGACGACT
GGACGATCCGCGAGGGCTCCTACTACCAGCTCACCACCGGCGGCGCCCCGCTCCGGAAGGCCGAGCCGTCGTTCGACGCC
GCGAAGACGCAATTGCGGCGGCTGCTGACCGAATCCGTCGAACAGCGCCTGGCGGCCTCGGACGTCCCGATCGCGACCTT
TCTCAGCGGCGGGCTTGATTCCTCGATCATCGCCAGCATTGCGGCCGGGATCGCCGGCGACCGGATGACGGCCTATTCGA
TCGGATTTCCCAACGATCCCGCGTTCGATGAAAGTCCGTATGCGCGATTGCTGGCCGCCCGCTATCCCCGGATCGACCAT
CGGGTGATCGACGTGACCGAGGAACGGCTCGCCGATTTCACCGACCGGACCCTCGGGCTCCTGGGCGAACCCTATGCCGA
TGCATCGCTGATTCCGACGGCCTTTCTGTGCTCGCATGTCGGCGAGAAGGTCATTCTGGGAGGCGATGGCGCCGATGAGC
TGTTCGCCGGATACGGCGTGTATTCGGCCATGCAGGTGAGTGCGCGCATCCCGGGCTGGATCAAGCGGGTGCTCCGCCGC
CTGCCGACACACGGCAATCCCGCCAGCATCGGCAACTCGAAGTTGCGGGCGGCGGCCTTCTTCCGCATGCATATGCGGGA
TACCGCGGGCGAGGAATATTTATCCTGGCGCTCCTACGCGTCGCCGGAGCAGTTGCAGTCGCTCGGGTTTTCGAACGCCG
ATGCGTGCGCGCTGCCGGGCGATCTCGTCGACCTCGACCTCCACACGCTGCGCGACATGCTGGTGACGGACATCCGCTTC
AATCTGCCGGCCGATATGCTGAAGAAGGTCGATCTGGCGAGCATGCAGCACGGACTCGAAGTGCGCTTGCCCTTTCTGGA
CTCGCGGCTGGTGGCGTTTGCGCTGGGGCTTCCCGACAAGTTCCTGATCAACGGCGGGGTGCGCAAACACATACTGCGCG
AGAGTTTCCGCTCCGACCTGCCGCCCGAAATCCTGACCCGGGGCAAGAAGGGATTCCTGCTGCCGATCCGCAAATGGATG
AGGGATGGCCGTATCCGCGACGAGCTTCTGGACCTGGCCGCCCGCCAGACCACGCTGGATTCGCGGGCCATCGAGCGATT
TGCGACCGAGCACCGCGAATTGCGAGCCGATCATTCCCAGCTGCTGTGGGCGTGCTATGTCTTTCTGAAATGGACGGGCA
GGCGAAACGGCAACCGGTCCGATCGGGGATCGATCAGCTCGGAGATCGCTGGATAA

Upstream 100 bases:

>100_bases
TGGGTCGCCGGGCGCGGCAGCGCGTTGAGGAGCAGTATTCGATCGCGGCGAATGTCGAGCGATATCACGCGATGTATGCG
GAACTGTTGGCGGGCGCCTG

Downstream 100 bases:

>100_bases
AATTCCGCAGCAGAGTATCGCATGGAATTCCAGTCGAGATCACCGCGGCACGACGAGACGCCGCCCCCTGATCCGGTCTC
TCAGTCTCGACATCGGCCCC

Product: asparagine synthase, glutamine-hydrolyzing

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 631; Mature: 631

Protein sequence:

>631_residues
MCGLAGGIWRDDRLLAPSIGAAALAALAHRGPDAEGEHREAGVFLGHRRLSIIDLDPRSTQPMHLGPLSIVFNGEIYNYR
ALRAELERGGCVFRTESDTEVLLQAFARDGLDCLQTLEGMFSFAIWDRSARRLTLARDRYGEKPMVVFEDGEKLLFASEI
PAIERLAGDGDLVIDRAALPLLFRFSYLPAPFTPFERMKQIKPGCWLQLTVDDWTIREGSYYQLTTGGAPLRKAEPSFDA
AKTQLRRLLTESVEQRLAASDVPIATFLSGGLDSSIIASIAAGIAGDRMTAYSIGFPNDPAFDESPYARLLAARYPRIDH
RVIDVTEERLADFTDRTLGLLGEPYADASLIPTAFLCSHVGEKVILGGDGADELFAGYGVYSAMQVSARIPGWIKRVLRR
LPTHGNPASIGNSKLRAAAFFRMHMRDTAGEEYLSWRSYASPEQLQSLGFSNADACALPGDLVDLDLHTLRDMLVTDIRF
NLPADMLKKVDLASMQHGLEVRLPFLDSRLVAFALGLPDKFLINGGVRKHILRESFRSDLPPEILTRGKKGFLLPIRKWM
RDGRIRDELLDLAARQTTLDSRAIERFATEHRELRADHSQLLWACYVFLKWTGRRNGNRSDRGSISSEIAG

Sequences:

>Translated_631_residues
MCGLAGGIWRDDRLLAPSIGAAALAALAHRGPDAEGEHREAGVFLGHRRLSIIDLDPRSTQPMHLGPLSIVFNGEIYNYR
ALRAELERGGCVFRTESDTEVLLQAFARDGLDCLQTLEGMFSFAIWDRSARRLTLARDRYGEKPMVVFEDGEKLLFASEI
PAIERLAGDGDLVIDRAALPLLFRFSYLPAPFTPFERMKQIKPGCWLQLTVDDWTIREGSYYQLTTGGAPLRKAEPSFDA
AKTQLRRLLTESVEQRLAASDVPIATFLSGGLDSSIIASIAAGIAGDRMTAYSIGFPNDPAFDESPYARLLAARYPRIDH
RVIDVTEERLADFTDRTLGLLGEPYADASLIPTAFLCSHVGEKVILGGDGADELFAGYGVYSAMQVSARIPGWIKRVLRR
LPTHGNPASIGNSKLRAAAFFRMHMRDTAGEEYLSWRSYASPEQLQSLGFSNADACALPGDLVDLDLHTLRDMLVTDIRF
NLPADMLKKVDLASMQHGLEVRLPFLDSRLVAFALGLPDKFLINGGVRKHILRESFRSDLPPEILTRGKKGFLLPIRKWM
RDGRIRDELLDLAARQTTLDSRAIERFATEHRELRADHSQLLWACYVFLKWTGRRNGNRSDRGSISSEIAG
>Mature_631_residues
MCGLAGGIWRDDRLLAPSIGAAALAALAHRGPDAEGEHREAGVFLGHRRLSIIDLDPRSTQPMHLGPLSIVFNGEIYNYR
ALRAELERGGCVFRTESDTEVLLQAFARDGLDCLQTLEGMFSFAIWDRSARRLTLARDRYGEKPMVVFEDGEKLLFASEI
PAIERLAGDGDLVIDRAALPLLFRFSYLPAPFTPFERMKQIKPGCWLQLTVDDWTIREGSYYQLTTGGAPLRKAEPSFDA
AKTQLRRLLTESVEQRLAASDVPIATFLSGGLDSSIIASIAAGIAGDRMTAYSIGFPNDPAFDESPYARLLAARYPRIDH
RVIDVTEERLADFTDRTLGLLGEPYADASLIPTAFLCSHVGEKVILGGDGADELFAGYGVYSAMQVSARIPGWIKRVLRR
LPTHGNPASIGNSKLRAAAFFRMHMRDTAGEEYLSWRSYASPEQLQSLGFSNADACALPGDLVDLDLHTLRDMLVTDIRF
NLPADMLKKVDLASMQHGLEVRLPFLDSRLVAFALGLPDKFLINGGVRKHILRESFRSDLPPEILTRGKKGFLLPIRKWM
RDGRIRDELLDLAARQTTLDSRAIERFATEHRELRADHSQLLWACYVFLKWTGRRNGNRSDRGSISSEIAG

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=401, Percent_Identity=25.9351620947631, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI168229252, Length=401, Percent_Identity=25.9351620947631, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI168229250, Length=401, Percent_Identity=25.9351620947631, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI296010848, Length=375, Percent_Identity=25.8666666666667, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1786889, Length=375, Percent_Identity=30.1333333333333, Blast_Score=117, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17560178, Length=397, Percent_Identity=29.9748110831234, Blast_Score=135, Evalue=9e-32,
Organism=Saccharomyces cerevisiae, GI6321563, Length=431, Percent_Identity=27.3781902552204, Blast_Score=94, Evalue=6e-20,
Organism=Drosophila melanogaster, GI45553209, Length=410, Percent_Identity=31.4634146341463, Blast_Score=126, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 70178; Mature: 70178

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGLAGGIWRDDRLLAPSIGAAALAALAHRGPDAEGEHREAGVFLGHRRLSIIDLDPRST
CCCCCCCCCCCCCEECCCHHHHHHHHHHHCCCCCCCCCCCCCEEEECCEEEEEECCCCCC
QPMHLGPLSIVFNGEIYNYRALRAELERGGCVFRTESDTEVLLQAFARDGLDCLQTLEGM
CCEEECCEEEEECCCEEEHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCCHHHHHHHHHH
FSFAIWDRSARRLTLARDRYGEKPMVVFEDGEKLLFASEIPAIERLAGDGDLVIDRAALP
HHHHHHCCCCCEEEEHHHHCCCCCEEEEECCCEEEEECCCCHHHHHCCCCCEEEEHHHHH
LLFRFSYLPAPFTPFERMKQIKPGCWLQLTVDDWTIREGSYYQLTTGGAPLRKAEPSFDA
HHHHHHCCCCCCCHHHHHHHCCCCCEEEEEECCEEECCCCEEEEECCCCCCCCCCCCHHH
AKTQLRRLLTESVEQRLAASDVPIATFLSGGLDSSIIASIAAGIAGDRMTAYSIGFPNDP
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECCCCCCC
AFDESPYARLLAARYPRIDHRVIDVTEERLADFTDRTLGLLGEPYADASLIPTAFLCSHV
CCCCCHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHC
GEKVILGGDGADELFAGYGVYSAMQVSARIPGWIKRVLRRLPTHGNPASIGNSKLRAAAF
CCEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
FRMHMRDTAGEEYLSWRSYASPEQLQSLGFSNADACALPGDLVDLDLHTLRDMLVTDIRF
HHHHHHHCCCHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHC
NLPADMLKKVDLASMQHGLEVRLPFLDSRLVAFALGLPDKFLINGGVRKHILRESFRSDL
CCCHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHCCCCHHEECCCHHHHHHHHHHHCCC
PPEILTRGKKGFLLPIRKWMRDGRIRDELLDLAARQTTLDSRAIERFATEHRELRADHSQ
CHHHHHCCCCCEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLWACYVFLKWTGRRNGNRSDRGSISSEIAG
HHHHHHHHHHEECCCCCCCCCCCCCHHHCCC
>Mature Secondary Structure
MCGLAGGIWRDDRLLAPSIGAAALAALAHRGPDAEGEHREAGVFLGHRRLSIIDLDPRST
CCCCCCCCCCCCCEECCCHHHHHHHHHHHCCCCCCCCCCCCCEEEECCEEEEEECCCCCC
QPMHLGPLSIVFNGEIYNYRALRAELERGGCVFRTESDTEVLLQAFARDGLDCLQTLEGM
CCEEECCEEEEECCCEEEHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCCHHHHHHHHHH
FSFAIWDRSARRLTLARDRYGEKPMVVFEDGEKLLFASEIPAIERLAGDGDLVIDRAALP
HHHHHHCCCCCEEEEHHHHCCCCCEEEEECCCEEEEECCCCHHHHHCCCCCEEEEHHHHH
LLFRFSYLPAPFTPFERMKQIKPGCWLQLTVDDWTIREGSYYQLTTGGAPLRKAEPSFDA
HHHHHHCCCCCCCHHHHHHHCCCCCEEEEEECCEEECCCCEEEEECCCCCCCCCCCCHHH
AKTQLRRLLTESVEQRLAASDVPIATFLSGGLDSSIIASIAAGIAGDRMTAYSIGFPNDP
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECCCCCCC
AFDESPYARLLAARYPRIDHRVIDVTEERLADFTDRTLGLLGEPYADASLIPTAFLCSHV
CCCCCHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHC
GEKVILGGDGADELFAGYGVYSAMQVSARIPGWIKRVLRRLPTHGNPASIGNSKLRAAAF
CCEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
FRMHMRDTAGEEYLSWRSYASPEQLQSLGFSNADACALPGDLVDLDLHTLRDMLVTDIRF
HHHHHHHCCCHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHC
NLPADMLKKVDLASMQHGLEVRLPFLDSRLVAFALGLPDKFLINGGVRKHILRESFRSDL
CCCHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHCCCCHHEECCCHHHHHHHHHHHCCC
PPEILTRGKKGFLLPIRKWMRDGRIRDELLDLAARQTTLDSRAIERFATEHRELRADHSQ
CHHHHHCCCCCEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLWACYVFLKWTGRRNGNRSDRGSISSEIAG
HHHHHHHHHHEECCCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]